Temperature-sensitive specific polypeptide and method for non-destructive separation of exosomes based on temperature difference specificity
Through the binding of the thermosensitive specific polypeptide with nanomagnetic microspheres, the temperature difference is used to regulate its affinity, and efficient, specific and non-destructive separation of exosomes is achieved, which solves the problems of exosome separation purity and integrity in the prior art, and achieves high-purity and non-damage exosome separation.
Patent Information
- Application Number
- CN202411597037.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-11
- Publication Date
- 2025-03-18
- Estimated Expiration
- 2044-11-11
AI Technical Summary
The lack of efficient and specific non-destructive separation methods for exosomes in the prior art has led to the hindering of the development of exosome-related research and application.
The temperature-sensitive specific polypeptide is modified with nanomagnetic microspheres, and its affinity is regulated through temperature difference to achieve specific binding and separation of exosomes. The specific steps include binding the nanomagnetic microspheres modified by the peptide to the exosome at low temperature, capturing and cleaning with a magnetic field, and then dissociating the peptide and the microspheres at high temperature to isolate the high-purity exosomes.
This method can significantly improve the purity of exosomes, avoid mechanical damage and structural damage during separation, and is easy to operate, low cost, and does not affect the morphological integrity of exosomes.