Method for fermenting cigar core tobacco leaves by adding exogenous enzyme preparation

Abstract

The invention discloses a method for fermenting cigar core tobacco leaves by adding an exogenous enzyme preparation. The method comprises the following steps: uniformly spraying distilled water to the tobacco leaves for moisture regaining; placing the dampened tobacco leaves in a constant-temperature and constant-humidity box, and balancing moisture for 24 hours under the conditions that the temperature is 25 DEG C and the humidity is 80% until the moisture content of the tobacco leaves is 22%; diluting the exogenous enzyme preparation to a required concentration by using distilled water to prepare an exogenous enzyme solution; uniformly spraying an exogenous enzyme preparation solution on the surfaces of the tobacco leaves subjected to moisture balancing, and balancing moisture for 24 hours in a constant-temperature and constant-humidity box under the conditions that the moisture regaining moisture content of the tobacco leaves is 26%, the temperature is 25 DEG C and the humidity is 80%; and putting the tobacco leaves subjected to moisture balance into a constant-temperature and constant-humidity box under the conditions that the temperature is 42 DEG C and the humidity is 80%, and fermenting for 7 days. According to the fermented tobacco leaves treated by the exogenous enzyme preparation, the quality characteristics of the tobacco leaves are improved, the aroma is richer, the aroma amount is increased, the aroma permeability is better, and offensive odor and irritation are reduced; the method plays an important role in improving the industrial availability of cigar core tobacco leaves.

Description

Technical Field

[0001] The invention relates to the technical field of cigar processing, and in particular to a method for adding exogenous enzyme preparations to cigar core tobacco leaves for fermentation. Background Art

[0002] The cigar core is the core part that determines the flavor and aroma style of the cigar when it is smoked, and has high requirements for the aroma style, flavor, strength and power of the tobacco leaves. However, most of the cigar core tobacco leaves produced in my country currently lack a strong aroma. During the tobacco leaf fermentation process, adding exogenous enzyme preparations can not only regulate the intrinsic chemical composition of the tobacco leaves, reduce the nicotine content, and improve the quality of the tobacco leaves caused by improper cultivation and curing measures, but also significantly increase the aroma in the tobacco leaves, reduce the impurities and irritation of the tobacco leaves, make the taste mellow and clean, and improve the overall quality of the tobacco leaves. It can also accelerate the fermentation process of the tobacco leaves and shorten the fermentation cycle of the tobacco leaves.

[0003] The main technical features of this technology are the types of exogenous enzyme preparations (lipoxygenase, polyphenol oxidase, peroxidase), the amount of addition, the pre-fermentation treatment process and the fermentation environment, especially the process of spraying 150U / g lipoxygenase on tobacco leaves and fermenting under the conditions of 42°C and 80%, which have distinguishing technical characteristics compared with the existing technology. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a method for fermenting cigar core tobacco leaves by adding exogenous enzyme preparations, which plays an important role in improving the aroma permeability of the core tobacco leaves, reducing impurities, and improving the industrial availability of domestic cigar core tobacco leaves.

[0005] The technical problem to be solved by the present invention is achieved through the following technical solutions:

[0006] A method for fermenting cigar filler tobacco leaves by adding exogenous enzyme preparations, comprising the following steps:

[0007] (1) Evenly spraying distilled water on the purchased tobacco leaves to moisten them;

[0008] (2) placing the moistened tobacco leaves in a constant temperature and humidity chamber at 25°C and 80% humidity for 24 hours to balance the moisture content of the tobacco leaves until the moisture content reaches 22%;

[0009] (3) diluting the exogenous enzyme preparation to a desired concentration with distilled water to prepare an exogenous enzyme solution;

[0010] (4) spraying the exogenous enzyme preparation solution evenly on the surface of the tobacco leaves after moisture balance, and re-humidifying the moisture content of the tobacco leaves to 26%, and then balancing the moisture content for 24 hours in a constant temperature and humidity chamber at a temperature of 25° C. and a humidity of 80%;

[0011] (5) The tobacco leaves after moisture balance are placed in a constant temperature and humidity chamber at a temperature of 42° C. and a humidity of 80% for fermentation. The entire fermentation time is 7 days.

[0012] Preferably, in the above technical solution, the exogenous enzyme preparation is one or more of lipoxygenase, polyphenol oxidase and peroxidase.

[0013] Preferably, in the above technical solution, the added amount of the exogenous enzyme preparation is 150 U / g.

[0014] Preferably, in the above technical solution, the tobacco leaves are the upper leaves of the cigar core produced in Wuyishan, Fujian.

[0015] A method for fermenting cigar filler tobacco leaves by adding exogenous enzyme preparations is disclosed and its use is in preparing cigars.

[0016] A tobacco leaf treated according to the above-mentioned method of adding exogenous enzyme preparation to the cigar filler tobacco leaf for fermentation.

[0017] A cigar, comprising the above-mentioned tobacco leaves.

[0018] Preferably, in the above technical solution, the cigar is 110 mm long and 14 mm in diameter.

[0019] The above technical solution of the present invention has the following beneficial effects:

[0020] The present application has found through experimental research that in the sensory quality evaluation of tobacco leaves fermented after being treated with exogenous enzyme preparations, the quality characteristics of tobacco leaves are improved, the aroma is richer, the aroma volume is increased, the aroma permeability is better, and the impurities and irritation are reduced. By comparison, it is found that the sensory quality evaluation result of tobacco leaves is the best after spraying 150U / g lipoxygenase on tobacco leaves and fermenting them under the conditions of 42°C and 80%. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate embodiments of the invention and, together with the description, serve to explain the principles of the invention.

[0022] Figure 1 This is a quality characteristic evaluation diagram of the products of each embodiment of the present application. DETAILED DESCRIPTION

[0023] Various exemplary embodiments of the present invention will now be described in detail with reference to the accompanying drawings. It should be noted that the relative arrangement of components and steps, numerical expressions and numerical values ​​set forth in these embodiments do not limit the scope of the present invention unless otherwise specifically stated.

[0024] Unless otherwise specified, the reagents used in this application are all commercially available or obtained through commercial channels, or can be prepared by referring to existing chemical methods.

[0025] Example 1

[0026] CK: The upper leaves of the cigar core from the Wuyishan production area in Fujian Province, Haiyan 201 (water content of about 18%), were evenly sprayed with distilled water to rehydrate the leaves. The rehydrated leaves were placed in a constant temperature and humidity chamber at 25°C and 80% humidity to balance the moisture for 24 hours until the moisture content of the leaves reached 22%.

[0027] After the moisture content of the tobacco leaves has been balanced, distilled water is evenly sprayed on the surface of the tobacco leaves until the moisture content of the tobacco leaves is regained to 26%. The moisture content is balanced for 24 hours in a constant temperature and humidity chamber at a temperature of 25°C and a humidity of 80%.

[0028] The tobacco leaves after moisture balance were fermented in a constant temperature and humidity chamber at 42°C and 80% humidity. The whole fermentation time was 7 days.

[0029] The tobacco leaves fermented for 7 days were rolled into cigars with a length of 110 mm and a diameter of 14 mm, and aged for 1 month in a Binder constant temperature and humidity chamber at a temperature of 18°C ​​and a relative humidity of 65% for evaluation.

[0030] The evaluation was conducted by experts from domestic cigar industry enterprises organized by the National Tobacco Cultivation Physiology and Biochemistry Research Base of Henan Agricultural University. The evaluation method adopted a 9-point system to evaluate the sensory quality of tobacco leaves, including aroma quality, aroma volume, aftertaste, miscellaneous smell, sweetness, irritation, burning, and gray. The full score is 100 points. The total score is calculated according to the following formula, and the result is rounded to one decimal place. The higher the total score, the better the sensory quality of the tobacco leaves.

[0031]

[0032] Where: T is the sensory quality evaluation score; Pi is the weight of a single index (aroma quality 18%, aroma volume 16%, aftertaste 16%, miscellaneous smell 10%, sweetness 10%, irritation 12%, flammability 10%, gray 8%; is the average score of a single indicator).

[0033] The evaluation criteria are shown in Table 1 below.

[0034] Table 1 Cigar tobacco leaf quality characteristics Sensory quality evaluation standards

[0035]

[0036] The tobacco leaf style characteristics were evaluated according to Tables 2 and 3:

[0037] Table 2 Sensory quality evaluation table of cigar tobacco style characteristics

[0038]

[0039] Table 3 Sensory quality evaluation criteria for cigar tobacco style characteristics

[0040]

[0041] Example 2

[0042] LOX: The upper leaves of the cigar core from the Wuyishan production area in Fujian Province, Haiyan 201 (water content of about 18%), were evenly sprayed with distilled water to rehydrate the leaves. The rehydrated leaves were placed in a constant temperature and humidity chamber at 25°C and 80% humidity to balance the moisture for 24 hours until the moisture content of the leaves reached 22%.

[0043] 150 U / g lipoxygenase solution was evenly sprayed on the surface of the tobacco leaves after moisture balance, and the moisture content of the tobacco leaves was regained to 26%. The moisture was balanced for 24 hours in a constant temperature and humidity chamber at a temperature of 25° C. and a humidity of 80%.

[0044] The tobacco leaves after moisture balance were fermented in a constant temperature and humidity chamber at 42°C and 80% humidity. The whole fermentation time was 7 days.

[0045] The sensory quality of the treated tobacco leaves was evaluated according to the evaluation method mentioned in Example 1.

[0046] Example 3

[0047] PPO: The upper leaves of the cigar core from Wuyishan, Fujian Province, Haiyan 201 (water content of about 18%) were sprayed evenly with distilled water to rehydrate them. The rehydrated tobacco leaves were placed in a constant temperature and humidity chamber at 25°C and 80% humidity to balance the moisture for 24 hours until the moisture content of the tobacco leaves reached 22%.

[0048] 150U / g polyphenol oxidase solution was evenly sprayed on the surface of the tobacco leaves after moisture balance, and the moisture content of the tobacco leaves was regained to 26%. The moisture was balanced for 24 hours in a constant temperature and humidity chamber at a temperature of 25°C and a humidity of 80%.

[0049] The tobacco leaves after moisture balance were fermented in a constant temperature and humidity chamber at 42°C and 80% humidity. The whole fermentation time was 7 days.

[0050] The sensory quality of the treated tobacco leaves was evaluated according to the evaluation method mentioned in Example 1.

[0051] Example 4

[0052] POD: The upper leaves of the cigar core from Wuyishan, Fujian Province, Haiyan 201 (water content of about 18%) were sprayed with distilled water evenly to rehydrate them. The rehydrated tobacco leaves were placed in a constant temperature and humidity chamber at 25°C and 80% humidity to balance the moisture for 24 hours until the moisture content of the tobacco leaves reached 22%.

[0053] After the moisture content of the tobacco leaves has been balanced, 150 U / g peroxide solution is evenly sprayed on the surface of the tobacco leaves, and the moisture content of the tobacco leaves is regained to 26%. The moisture content is balanced for 24 hours in a constant temperature and humidity chamber at a temperature of 25° C. and a humidity of 80%.

[0054] The tobacco leaves after moisture balance were fermented in a constant temperature and humidity chamber at 42°C and 80% humidity. The whole fermentation time was 7 days.

[0055] The sensory quality of the treated tobacco leaves was evaluated according to the evaluation method mentioned in Example 1.

[0056] The sensory quality evaluation results of Examples 1-4 are as follows Figure 1 As shown in Tables 4 and 5:

[0057] Table 4 Evaluation table of fragrance characteristics of various examples

[0058]

[0059] Table 5 Evaluation table of style features of various embodiments

[0060]

[0061] After treatment with 150U / g lipoxygenase, the aroma quality of the tobacco leaves is improved, the aroma volume is increased, the aftertaste is more comfortable, and the sweetness is enhanced; in terms of style characteristics, the aroma richness of the treated tobacco leaves is increased, the woody aroma is more significant, the pollen aroma is prominent, and the bean aroma, honey sweet aroma and resin aroma are all increased compared with the untreated tobacco leaves; the green impurities in the treated tobacco leaves are reduced, the smoke concentration is increased, the smoke intensity is also increased, the permeability is better, the mellowness is increased, and the cigar style is more prominent.

[0062] After being treated with 150U / g polyphenol oxidase, the aftertaste is more comfortable, the sweetness is enhanced, and the combustibility is slightly improved; in terms of style characteristics, the aroma of the treated tobacco leaves is richer, the woody and bean aromas are more permeable, and there is a slight pollen and milk aroma; the smoke concentration of the treated tobacco leaves is increased, the smoke intensity is also increased, and the mellowness is increased.

[0063] After treatment with 150U / g peroxide, the impurities decreased and the combustibility was slightly improved; in terms of style characteristics, the bean aroma was improved and there was a slight baking aroma; the smoke concentration and smoke intensity of the treated tobacco leaves increased, and the mellowness increased.

[0064] Although the present invention has been disclosed as above by the embodiments, it is not intended to limit the present invention. Any person skilled in the art may make various choices and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention is limited by the claims and their equivalents.

Claims

1. A method for fermenting cigar filler tobacco leaves by adding exogenous enzyme preparations, characterized in that: The following steps are involved: (1) Spray distilled water evenly on the tobacco leaves to moisten them; (2) placing the moistened tobacco leaves in a constant temperature and humidity chamber at 25°C and 80% humidity for 24 hours to balance the moisture content of the tobacco leaves until the moisture content reaches 22%; (3) diluting the exogenous enzyme preparation to a desired concentration with distilled water to prepare an exogenous enzyme solution; (4) spraying the exogenous enzyme preparation solution evenly on the surface of the tobacco leaves after moisture balance, and re-humidifying the tobacco leaves to a moisture content of 26%, and then balancing the moisture for 24 hours in a constant temperature and humidity chamber at a temperature of 25° C. and a humidity of 80%; (5) The tobacco leaves after moisture balance are placed in a constant temperature and humidity chamber for fermentation at a temperature of 42° C. and a humidity of 80%. The entire fermentation time is 7 days.

2. The method for fermenting cigar filler tobacco leaves by adding exogenous enzyme preparations according to claim 1, characterized in that: The exogenous enzyme preparation is one or more of lipoxygenase, polyphenol oxidase and peroxidase.

3. The method for fermenting cigar filler tobacco leaves by adding exogenous enzyme preparations according to claim 2, characterized in that: The added amount of the exogenous enzyme preparation is 150 U / g.

4. The method for fermenting cigar filler tobacco leaves by adding exogenous enzyme preparations according to claim 1, characterized in that: The tobacco leaves are the upper leaves of the cigar core produced in Wuyishan, Fujian.

5. Use of the fermentation method of cigar filler tobacco leaves with exogenous enzyme preparation added according to any one of claims 1 to 4 in preparing cigars.

6. A tobacco leaf treated by the method of fermenting cigar filler tobacco leaves by adding exogenous enzyme preparation according to any one of claims 1 to 4.

7. A cigar, characterized in that: The cigar contains the tobacco leaf according to claim 6.

8. The cigar according to claim 7, characterized in that: The cigar is 110 mm long and 14 mm in diameter.

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