Method for improving transpiration resistance of grapes, anti-transpiration agent for grapes as well as preparation method and application of anti-transpiration agent

Abstract

The invention relates to the technical field of plant planting, and particularly discloses a method for improving the transpiration resistance of grapes, a grape transpiration-resistant agent and a preparation method and application of the grape transpiration-resistant agent. The method comprises the following steps that the grape transpiration-resistant agent is prepared, specifically, an active component A and a film-forming component B are prepared respectively; spraying the active component A on the front and back surfaces of grape leaves when the grapes enter a color turning early stage (EL-35), and then spraying the same amount of the film-forming component B on the front and back surfaces of the grape leaves; spraying the active component A on the front and back surfaces of grape leaves when the grapes enter a later color turning stage (EL-37), and then spraying the same amount of the film-forming component B on the front and back surfaces of the grape leaves. The grape anti-transpiration agent provided by the invention is used for reducing transpiration of grape leaves, improving the photosynthetic rate of grapes, increasing the size of grapes under drought stress and reducing the influence of the drought stress on the chromaticity of grape peels.

Description

Technical Field

[0001] The invention relates to the technical field of plant planting, and in particular to a method for improving the anti-transpiration ability of grapes, a grape anti-transpiration agent, and a preparation method and application thereof. Background Art

[0002] Drought affects the growth and development of grapes. Excessive drought can cause the leaves of grapes to wilt, turn yellow and fall off, or even cause the tree to die, leading to a decline in the quality of grape fruits and a reduction in yield, which will ultimately affect the quality of the wine and hinder the sustainable development of the wine industry.

[0003] The main way for plants to cope with drought stress is to reduce the degree of stomatal opening and reduce transpiration, thereby ensuring that the water content of the plant is within the normal range and maintaining plant growth. However, the reduction of stomatal conductance will affect CO2 fixation and photosynthesis. Therefore, it is urgent to provide a method to improve the transpiration resistance of grapes. Summary of the invention

[0004] In order to develop an anti-transpiration agent that can maintain the normal growth and development of grape trees, the present invention provides a method for improving the anti-transpiration ability of grapes, a grape anti-transpiration agent, and a preparation method and application thereof. The grape anti-transpiration agent provided by the present invention is used to reduce the transpiration of grape leaves, improve the photosynthetic rate of grapes, increase the fruit size of grapes under drought stress, and reduce the effect of drought stress on the chromaticity of grape skin.

[0005] The present invention provides a method for improving the transpiration resistance of grapes, comprising the following steps:

[0006] Preparation of grape anti-transpiration agent: prepare active ingredient A and film-forming component B separately;

[0007] The formula of active ingredient A is: fulvic acid 0.7g / L~0.9g / L, putrescine 4mg / L~10mg / L, 24-epibrassinolide 0.09mg / L~0.11mg / L, and the solvent is water; the formula of film-forming component B is: calcium chloride 1.5g / L~2.5g / L, glucose 5g / L~7g / L, and the solvent is water;

[0008] When the grapes enter the early stage of color change (EL-35), the active ingredient A is sprayed on the front and back sides of the grape leaves, and the spraying amount is 150mL to 300mL. Then, an equal amount of film-forming component B is sprayed on the front and back sides of the grape leaves to form a grape anti-transpiration agent in which the active ingredient A is wrapped by the film-forming component B.

[0009] When the grapes enter the late color change stage (EL-37), spray the active ingredient A on the front and back sides of the grape leaves at a spraying amount of 150mL to 300mL, and then spray an equal amount of film-forming component B on the front and back sides of the grape leaves.

[0010] The present invention adopts the method to first spray the active ingredient A on the surface of plant leaves, and then spray an equal volume of film-forming component B on the surface of plant leaves to form a grape anti-transpiration agent in which the active ingredient A is wrapped by the film-forming component B, thereby reducing the transpiration of grape leaves, improving the photosynthetic rate of grapes, increasing the fruit size of grapes under drought stress, and reducing the influence of drought stress on the chromaticity of grape skin.

[0011] Furthermore, the active ingredient A has a formula of: 0.8 g / L fulvic acid, 4.41 mg / L putrescine, 0.1 mg / L 24-epibrassinolide, and the solvent is water.

[0012] Furthermore, the film-forming component B has a formula of: 2 g / L calcium chloride, 6 g / L glucose, and the solvent is water.

[0013] The invention also provides a grape anti-transpiration agent, which consists of the active ingredient A and a film-forming component B.

[0014] Furthermore, the active ingredient A has a formula of: 0.8 g / L fulvic acid, 4.41 mg / L putrescine, 0.1 mg / L 24-epibrassinolide, and the solvent is water.

[0015] Furthermore, the film-forming component B has a formula of: 2 g / L calcium chloride, 6 g / L glucose, and the solvent is water.

[0016] The present invention also provides an application of the grape anti-transpiration agent in grape planting. The grape anti-transpiration agent is sprayed once when the grapes enter the early color change stage (EL-35) and the late color change stage (EL-37), and the spraying amount each time is 150mL to 300mL.

[0017] Furthermore, the spraying process is: spraying the grape anti-transpiration agent on the front and back sides of the grape leaves, first spraying the active ingredient A on the surface of the plant leaves, and then spraying an equal volume of the film-forming component B on the surface of the plant leaves, that is, forming a grape anti-transpiration agent in which the active ingredient A is wrapped by the film-forming component B.

[0018] Furthermore, the grape anti-transpiration agent is used to reduce the transpiration of grape leaves, improve the photosynthetic rate of grapes, increase the fruit size of grapes under drought stress, and reduce the effect of drought stress on the chromaticity of grape skin.

[0019] Furthermore, increasing the grape berry size under drought stress means that the horizontal diameter, vertical diameter and 100-grain weight of the grape berries all increase.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The method for improving the anti-transpiration ability of grapes provided by the present invention comprises the following steps: when the grapes enter the early stage of color change (EL-35), the active ingredient A is sprayed on the front and back sides of the grape leaves, the spraying amount is 150mL-300mL, and then an equal amount of film-forming component B is sprayed on the front and back sides of the grape leaves to form a grape anti-transpiration agent in which the active ingredient A is wrapped by the film-forming component B, and when the grapes enter the late stage of color change (EL-37), the active ingredient A is sprayed on the front and back sides of the grape leaves, the spraying amount is 150mL-300mL, and then an equal amount of film-forming component B is sprayed on the front and back sides of the grape leaves. The transpiration of grape leaves is significantly reduced, the photosynthetic rate of grapes is improved, the fruit size of grapes under drought stress is increased, and the influence of drought stress on the chromaticity of grape skin is reduced.

[0022] The present invention also provides an anti-transpiration agent, which can alleviate the influence of drought stress on grape growth and improve grape leaf transpiration and fruit quality, and has the advantages of being green, safe, pollution-free, and having good effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0024] Figure 1 Relative soil water content;

[0025] In the figure, A is the relative soil water content before spraying the grape anti-transpiration agent at the EL35 stage in Example 1;

[0026] B is the relative soil moisture content before spraying the grape anti-transpiration agent at the EL-37 stage in Example 1.

[0027] Figure 2 The effects of different treatments on the transpiration rate and photosynthesis rate of grapes;

[0028] In the figure, A shows the effect of different treatments on the transpiration rate of grapes during the EL-35 period;

[0029] B is the effect of different treatments on the transpiration rate of grapes during the EL-37 period.

[0030] Figure 3 The effects of different treatments on the photosynthetic rate of grapes;

[0031] In the figure, A shows the effects of different treatments on the photosynthetic rate of grapes during the EL-35 period;

[0032] B is the effect of different treatments on the photosynthetic rate of grapes during the EL-37 period.

[0033] Figure 4 The effect of different treatments on relative water use efficiency of leaves;

[0034] In the figure, A shows the effect of different treatments on relative water use efficiency of leaves during the EL-35 period;

[0035] B is the effect of different treatments on the relative water use efficiency of leaves during the EL-37 period.

[0036] Figure 5 The sugar and acid content of grapes in different treatments during harvesting period;

[0037] In the figure, A shows the effect of different treatments at harvest time on the reducing sugar content of grape fruit;

[0038] B shows the effect of different treatments at harvest time on the titratable acidity of grape berries. DETAILED DESCRIPTION

[0039] The specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention. The experimental methods described in the embodiments of the present invention are conventional methods unless otherwise specified, and the materials, reagents, etc. used in the following embodiments can be obtained from commercial channels unless otherwise specified.

[0040] Embodiment 1: A grape anti-transpiration agent and a method for improving the anti-transpiration ability of grapes.

[0041] 1. Grape anti-transpiration agent

[0042] The grape anti-transpiration agent comprises an active ingredient A and a film-forming component B;

[0043] The formula of active ingredient A is: fulvic acid 0.8g / L, putrescine 4.41mg / L, 24-epicrassinolide 0.1mg / L, and the solvent is water;

[0044] The formula of film-forming component B is: calcium chloride 2g / L, glucose 6g / L, and the solvent is water.

[0045] 2. Preparation method of grape anti-transpiration agent

[0046] (1) Preparation of active ingredient A: Taking 1L as an example, measure 800mL of deionized water and pour it into a beaker, accurately weigh 0.8g of fulvic acid and 4.41mg of putrescine, dissolve the above two substances with a little deionized water, and then pour the solution into the beaker, and stir the mixed liquid thoroughly to make all the ingredients evenly distributed. It is recommended to store at low temperature. Prepare 100mL of 50mg / L brassinolide mother solution: put 5mg of brassinolide powder into a beaker or centrifuge tube, add a small amount of solvent (ethanol or DMSO, a few microliters to tens of microliters), stir or shake to completely dissolve brassinolide, and transfer the dissolved brassinolide solution to a 100mL measuring cylinder to obtain the mother solution. Add 2mL of the mother solution to the above 1L beaker, mix thoroughly and make up to 1L with deionized water.

[0047] (2) Preparation of film-forming component B: Taking 1L as an example, measure 800mL of deionized water and pour it into a beaker. Use an electronic balance to weigh 2g of calcium chloride, add it to the water, and stir with a glass rod or a stirrer until the calcium chloride is completely dissolved. Use an electronic balance to weigh 6g of glucose and add it to the calcium chloride solution. Stir with a glass rod or a stirrer until the glucose is completely dissolved. Pour the solution into a volumetric flask and make up to 1L with deionized water. Stir the mixed liquid thoroughly so that all components are evenly distributed. Store at room temperature.

[0048] Embodiment 2: A grape anti-transpiration agent and a method for improving the anti-transpiration ability of grapes.

[0049] 1. Grape anti-transpiration agent

[0050] The grape anti-transpiration agent comprises an active ingredient A and a film-forming component B;

[0051] The formula of active ingredient A is: fulvic acid 0.8g / L, putrescine 4.41mg / L, 24-epicrassinolide 0.15mg / L, and the solvent is water;

[0052] The formula of film-forming component B is: calcium chloride 2g / L, glucose 6g / L, and the solvent is water.

[0053] 2. Preparation method of grape anti-transpiration agent

[0054] (1) Preparation of active ingredient A: Taking 1L as an example, measure 800mL of deionized water and pour it into a beaker, accurately weigh 0.8g of fulvic acid and 4.41mg of putrescine, dissolve the above two substances with a little deionized water, and then pour the solution into the beaker to prepare 100mL of 50mg / L brassinolide mother solution: put 5mg of brassinolide powder into a beaker or centrifuge tube, add a small amount of solvent (ethanol or DMSO, a few microliters to tens of microliters), stir or shake to completely dissolve brassinolide, and transfer the dissolved brassinolide solution to a 100mL measuring cylinder to obtain the mother solution. Add 3mL of the mother solution to the above 1L beaker, mix thoroughly and dilute to 1L with deionized water.

[0055] (2) The preparation process of film-forming component B is consistent with that in Example 1.

[0056] Embodiment 3: A grape anti-transpiration agent and a method for improving the anti-transpiration ability of grapes.

[0057] 1. Grape anti-transpiration agent

[0058] The grape anti-transpiration agent comprises an active ingredient A and a film-forming component B;

[0059] The formula of active ingredient A is: fulvic acid 0.8g / L, putrescine 8.82mg / L, 24-epibrassinolide 0.1mg / L, and the solvent is water;

[0060] The formula of film-forming component B is: calcium chloride 2g / L, glucose 6g / L, and the solvent is water.

[0061] 2. Preparation method of grape anti-transpiration agent

[0062] (1) Preparation of active ingredient A:

[0063] Taking 1L as an example, measure 800mL of deionized water and pour it into a beaker, accurately weigh 0.8g of fulvic acid and 8.82mg of putrescine, dissolve the above two substances with a little deionized water, then pour the solution into the beaker, stir the mixed liquid thoroughly to make all the ingredients evenly distributed. It is recommended to store at low temperature. Prepare 100mL of 50mg / L brassinolide mother solution: put 5mg of brassinolide powder into a beaker or centrifuge tube, add a small amount of solvent (ethanol or DMSO, a few microliters to tens of microliters), stir or shake to completely dissolve brassinolide, and transfer the dissolved brassinolide solution to a 100mL measuring cylinder to obtain the mother solution. Add 2mL of the mother solution to the above 1L beaker, mix thoroughly and dilute to 1L with deionized water.

[0064] (2) The preparation process of film-forming component B is consistent with that in Example 1.

[0065] Embodiment 4: A grape anti-transpiration agent and a method for improving the anti-transpiration ability of grapes.

[0066] 1. Grape anti-transpiration agent

[0067] The grape anti-transpiration agent comprises an active ingredient A and a film-forming component B;

[0068] The formula of active ingredient A is: fulvic acid 0.8g / L, putrescine 8.82mg / L, 24-epicrassinolide 0.15mg / L, and the solvent is water;

[0069] The formula of film-forming component B is: calcium chloride 2g / L, glucose 6g / L, and the solvent is water.

[0070] 2. Preparation method of grape anti-transpiration agent

[0071] (1) Preparation of active ingredient A:

[0072] Taking 1L as an example, measure 800mL of deionized water and pour it into a beaker, accurately weigh 0.8g of fulvic acid and 8.82mg of putrescine, dissolve the above two substances with a little deionized water, then pour the solution into the beaker, stir the mixed liquid thoroughly to make all the ingredients evenly distributed. It is recommended to store at low temperature. Prepare 100mL of 50mg / L brassinolide mother solution: put 5mg of brassinolide powder into a beaker or centrifuge tube, add a small amount of solvent (ethanol or DMSO, a few microliters to tens of microliters), stir or shake to completely dissolve brassinolide, and transfer the dissolved brassinolide solution to a 100mL measuring cylinder to obtain the mother solution. Add 3mL of the mother solution to the above 1L beaker, mix thoroughly and dilute to 1L with deionized water.

[0073] (2) The preparation process of film-forming component B is consistent with that in Example 1.

[0074] The specific process of applying the obtained grape anti-transpiration agent to grapes in the above-mentioned Examples 1 to 4 is as follows: when the grapes enter the early stage of color change (EL-35) and the late stage of color change (EL-37), the prepared grape anti-transpiration agent is sprayed on the front and back sides of the grape leaves with a sprayer. The spraying time is selected after dusk every day to prevent violent evaporation, so that the leaf surface is covered with water droplets but not dripping (the dosage range is controlled to be 150mL to 300mL according to the size of the leaves). The active ingredient A is first sprayed on the surface of the plant leaves, and then an equal volume of the film-forming component B is sprayed on the surface of the plant leaves, that is, the grape anti-transpiration agent in which the active ingredient A is wrapped by the film-forming component B is formed. The grape anti-transpiration agent prepared in Examples 1 to 4 is sprayed once in the early stage of color change (EL-35) and the late stage of color change (EL-37) during use.

[0075] Example 5: A grape anti-transpiration agent and a method for improving the anti-transpiration ability of grapes.

[0076] The formula and preparation method of the grape anti-transpiration agent are the same as those in Example 1; the difference is that during the spraying process, it is sprayed only once in the early stage of grape color change (EL-35), and the spraying amount is 100 mL, based on the leaf surface being covered with water droplets but not dripping.

[0077] Example 6: A grape anti-transpiration agent and a method for improving the anti-transpiration ability of grapes.

[0078] The formula and preparation method of the grape anti-transpiration agent are the same as those in Example 2; the difference is that during the spraying process, it is sprayed only once in the early stage of grape color change (EL-35), and the spraying amount is 100 mL, based on the leaf surface being covered with water droplets but not dripping.

[0079] Example 7: A grape anti-transpiration agent and a method for improving the anti-transpiration ability of grapes.

[0080] The formula and preparation method of the grape anti-transpiration agent are the same as those in Example 3; the difference is that during the spraying process, it is sprayed only once in the early stage of grape color change (EL-35), and the spraying amount is 100 mL, based on the leaf surface being covered with water droplets but not dripping.

[0081] Example 8: A grape anti-transpiration agent and a method for improving the anti-transpiration ability of grapes.

[0082] The formula and preparation method of the grape anti-transpiration agent are the same as those in Example 4; the difference is that during the spraying process, it is sprayed only once in the early stage of grape color change (EL-35), and the spraying amount is 100 mL, based on the leaf surface being covered with water droplets but not dripping.

[0083] Comparative Example 1: clean water was used instead of the anti-transpiration agent prepared in Example 1, and normal irrigation was carried out according to the vineyard management method, and the implementation standard was based on the cultivation management standard of the Ningxia Helan Mountain East Foothill Wine Producing Area.

[0084] Comparative Example 2: Drought treatment was carried out, that is, no artificial irrigation was carried out during the entire test period. At the same time, the soil moisture content was measured and monitored to ensure that the grapes grew in drought conditions.

[0085] Comparative Example 3: A commercial anti-transpiration agent was used instead of the anti-transpiration agent in Example 1 (Guoguang Anti-transpiration Agent, fertilizer grade number: Agricultural Fertilizer (2013) Standard No. 3202, implementation standard number, NY 1106), and the usage method was referred to its instruction manual.

[0086] The grape anti-transpiration agent and method prepared in the above-mentioned Examples 1 to 8 and Comparative Examples 1 to 3 were used to treat grapes to study the effect of the grape anti-transpiration agent on the anti-transpiration effect and growth and development of grapes. The details are as follows:

[0087] 1. Experimental Methods

[0088] 1. Test grape variety: Eurasian wine grape "Cabernet Sauvignon" (Vitis vinifera L. Cabernet Sauvignon). The selected grape trees are 4 years old and are cultivated using the "F"-shaped trellis method. The middle and long shoots are pruned, the row spacing is 0.8m×3m, the cultivation row direction is north-south, and drip irrigation is used for irrigation.

[0089] 2. Experimental processing steps

[0090] The vineyard was treated according to the methods of Examples 1 to 8 and Comparative Examples 1 to 3, and three rows of grape plants with uniform growth and no pests and diseases were selected, and were marked as row A, row B, and row C, respectively. There was one row between the three rows, row A was irrigated normally, and rows B and C were drought treated. Among them, Comparative Example 1 was in row A, Comparative Example 2 and Examples 1-4 were in row B, and Comparative Example 3 and Examples 5-8 were in row C.

[0091] 3. Index determination

[0092] (1) Determination of soil moisture content: The zigzag sampling method was used in the three-row test plot selected for the experiment. According to the method of 3 sampling points / row, one day before each spraying, soil samples at 0-20 cm and 20-40 cm were obtained using a soil sampler. The fresh weight was weighed, and the soil was naturally dried to a constant weight. The dry soil was weighed and the mass moisture content was calculated. At the same time, the ring knife method was used to determine the field water holding capacity. Finally, the relative moisture content was obtained according to the definition.

[0093] (2) Grape leaf photosynthesis measurement: After each spraying, the net photosynthetic rate (A), transpiration rate (E), stomatal conductance (Gs), and intercellular carbon dioxide concentration (Ci) of the leaves were measured using a GFS-3000 photosynthetic meter (WALZ, Germany) from 8:30 to 11:30 the next morning (in clear weather). The measurement method was: four grape trees were randomly selected for each treatment, and three leaves were randomly selected from each tree to measure photosynthetic characteristics. The selection of leaves for measurement followed the principle of being free of pests and diseases, and being basically the same in size, growth height, and maturity.

[0094] (3) Sampling method: At fruit EL-38 (harvest period), fruit clusters with relatively consistent growth and free of pests and diseases from different treatments were collected and placed in a -40°C refrigerator for subsequent fruit quality determination.

[0095] (4) Data processing method: SPSS27 statistical software was used for statistical analysis of the data, and one-way analysis of variance (ANOVA) and Duncan's new multiple range method were used for multiple comparisons to detect the significant differences between different treatments (P<0.05).

[0096] 2. Experimental Results

[0097] 1. Determination of soil moisture content

[0098] The relative water content of the soil in three rows was measured before spraying grape anti-transpiration agent at the EL-35 and EL-37 stages.

[0099] Depend on Figure 1 It can be seen that after drought treatment, the relative soil moisture content in the EL-35 period decreased by about 25% compared with the normal irrigation treatment, and in the EL-37 period it decreased by about 32%, which is basically in moderate drought.

[0100] 2. Effect of the grape anti-transpiration agent prepared by the present invention on the anti-transpiration ability and photosynthetic rate of grapes

[0101] Depend on Figure 2 It can be seen that in the EL-35 period, compared with the CK treatment (Comparative Example 1), the drought treatment (Comparative Example 2) significantly reduced the transpiration of the leaves. After spraying the grape anti-transpiration agent, Examples 1 to 4 and 6 significantly reduced the transpiration. The grape anti-transpiration agent prepared in Example 3 had a higher effect after spraying than the commercial anti-transpiration agent in Comparative Example 5. In the EL-37 period, Examples 1, 3 and 8 significantly reduced the transpiration, but the decline was lower than that in the EL-35 period.

[0102] Depend on Figure 3 It can be seen that compared with the drought treatment during the EL-35 period, Examples 1 and 3 significantly increased the photosynthetic rate, and compared with the drought treatment during the EL-37 period, Examples 1 and 4 significantly increased the photosynthetic rate.

[0103] 3. The influence of the grape anti-transpiration agent prepared by the present invention on water utilization efficiency.

[0104] Depend on Figure 4 It can be seen that compared with the CK (Comparative Example 1) treatment, the reducing sugar content of the fruit under drought conditions (Comparative Example 2) increased significantly, and the reducing sugar content also increased significantly between different treatments, and the reducing sugar content in Example 3 increased most significantly. In terms of titratable acid, the titratable acidity also decreased accordingly due to the increase in reducing sugar content.

[0105] 4. Effect of the grape anti-transpiration agent prepared by the present invention on the reducing sugar content and titratable acidity.

[0106] like Figure 5 As shown, compared with the CK (Comparative Example 1) treatment, the reducing sugar content of the fruit under drought conditions (Comparative Example 2) increased significantly, and the reducing sugar content also increased significantly between different treatments, and the reducing sugar content in Example 3 increased most significantly. In terms of titratable acid, the titratable acidity also decreased accordingly due to the increase in reducing sugar content.

[0107] 5. Effect of the grape anti-transpiration agent prepared by the present invention on fruit size.

[0108] Table 1 Effects of antitranspiration agents on Cabernet Sauvignon fruit size

[0109] deal with Horizontal diameter(mm) Longitudinal diameter(mm) 100-grain weight (g) Comparative Example 1 (CK) 12.88±0.42ba 12.84±0.34a 127.51±5.79c Comparative Example 2 (Dry) 11.41±1.04c 11.45±0.96c 91.22±3.38g Example 1 12.06±0.15cb 12.01±0.15cb 104.64±1.13f Example 2 13.21±0.23a 13.26±0.32a 144.60±0.92a Example 3 12.69±0.11ba 12.70±0.12ba 114.43±1.01e Example 4 13.09±0.51a 13.12±0.42a 124.83±2.84cd Comparative Example 3 13.07±0.59a 13.04±0.65a 134.83±1.19b Example 5 13.02±0.27a 12.98±0.13a 121.84±2.04d Example 6 13.01±0.25a 12.97±0.17a 125.67±3.29cd Example 7 12.06±0.15cb 12.01±0.14cb 102.07±1.46f Example 8 12.57±0.48ba 12.55±0.52ba 138.85±0.42b

[0110] As can be seen from Table 1, compared with CK (Comparative Example 1), drought treatment (Comparative Example 2) reduced the fruit size. In terms of fruit size, compared with drought treatment, different embodiments and Comparative Example 3 increased the fruit size to a certain extent, but there was no significant difference compared with CK. To a certain extent, it shows that spraying anti-transpiration agents under drought stress can improve fruit size.

[0111] Table 2 Effect of anti-transpiration agents on grape skin chromaticity

[0112]

[0113] It can be seen from Table 2 that in terms of color brightness, compared with the drought treatment, the spraying treatments of Examples 2 to 9 can reduce the peel brightness L. In terms of red-green hue a*, the drought treatment causes the peel to be more inclined to red, and Examples 1 to 3 and Examples 6 to 8 cause the peel color tone to be inclined to Comparative Example 1 (CK).

[0114] Based on the evaluation of various indicators, the preferred embodiment of the present invention is Embodiment 3, and the specific formula is the active ingredient A formula: 0.8 g / L fulvic acid, 8.82 mg / L putrescine, 0.1 mg / L 24-epibrassinolide, and the solvent is water; the film-forming component B formula: 2 g / L calcium chloride, 6 g / L glucose, and the solvent is water.

[0115] Although preferred embodiments of the present invention have been described, additional changes and modifications may occur to these embodiments once those skilled in the art are aware of the basic inventive concepts.

[0116] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A method for improving the transpiration resistance of grapes, characterized in that: The steps include: Preparation of grape anti-transpiration agent: prepare active ingredient A and film-forming component B separately; The formula of active ingredient A is: fulvic acid 0.7g / L~0.9g / L, putrescine 4mg / L~10mg / L, 24-epibrassinolide 0.09mg / L~0.11mg / L, and the solvent is water; the formula of film-forming component B is: calcium chloride 1.5g / L~2.5g / L, glucose 5g / L~7g / L, and the solvent is water; When the grapes enter the early stage of color change, the active ingredient A is sprayed on the front and back sides of the grape leaves at a spraying amount of 75mL to 150mL, and then an equal amount of film-forming component B is sprayed on the front and back sides of the grape leaves to form a grape anti-transpiration agent in which the active ingredient A is wrapped by the film-forming component B; When the grapes enter the late color change stage, spray the active ingredient A on the front and back sides of the grape leaves at a spraying amount of 150mL to 300mL, and then spray an equal amount of film-forming component B on the front and back sides of the grape leaves.

2. The method for improving the transpiration resistance of grapes according to claim 1, characterized in that: The active ingredient A has a formula of: 0.8 g / L fulvic acid, 4.41 mg / L putrescine, 0.1 mg / L 24-epibrassinolide, and the solvent is water.

3. The method for improving the transpiration resistance of grapes according to claim 1, characterized in that: The film-forming component B has a formula of: 2 g / L calcium chloride, 6 g / L glucose, and the solvent is water.

4. A grape anti-transpiration agent, characterized in that: The grape anti-transpiration agent consists of the active ingredient A described in claim 1 and a film-forming component B.

5. The grape anti-transpiration agent according to claim 4, characterized in that: The active ingredient A has a formula of: 0.8 g / L fulvic acid, 4.41 mg / L putrescine, 0.1 mg / L 24-epibrassinolide, and the solvent is water.

6. The grape anti-transpiration agent according to claim 4, characterized in that: The film-forming component B has a formula of: 2 g / L calcium chloride, 6 g / L glucose, and the solvent is water.

7. Use of the grape anti-transpiration agent according to any one of claims 4 to 6 in grape planting, characterized in that: Grape anti-transpiration agent should be sprayed once in the early and late stages of color change, with each spraying amount being 150mL to 300mL.

8. The use of the grape anti-transpiration agent in grape planting according to claim 7, characterized in that: The spraying process is: spray the grape anti-transpiration agent on the front and back sides of the grape leaves, first spray the active ingredient A on the surface of the plant leaves, and then spray an equal volume of film-forming component B on the surface of the plant leaves, that is, forming a grape anti-transpiration agent in which the active ingredient A is wrapped by the film-forming component B.

9. The use of the grape anti-transpiration agent in grape planting according to claim 7, characterized in that: The grape anti-transpiration agent is used to reduce the transpiration of grape leaves, improve the photosynthetic rate of grapes, increase the fruit size of grapes under drought stress, and reduce the influence of drought stress on the chromaticity of grape skin.

10. The use of the grape anti-transpiration agent in grape planting according to claim 9, characterized in that: Increasing the fruit size of grapes under drought stress means that the horizontal diameter, vertical diameter and 100-grain weight of the grapes all increase.

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