Anoectochilus roxburghii polysaccharide and its use in preparing cartilage damage repair medicine
Through optimization of the process and multiple structural characterization methods, four polysaccharides were purified and identified from the zebrafish produced in Zhejiang, and their significant cartilage damage repair effect was verified in the transgenic zebrafish model, solving the shortcomings of the research on the zebrafish polysaccharides produced in the existing technology.
CN119350524BActive Publication Date: 2025-04-04ZHEJIANG UNIV OF TECH
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Patent Information
- Application Number
- CN202411910902.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-24
- Publication Date
- 2025-04-04
- Estimated Expiration
- 2044-12-24
AI Technical Summary
Technical Problem
The prior art is difficult to effectively isolate and structure the identification of the polysaccharides of zhema, and its biological activity in cartilage injury repair is rarely studied.
Method used
By optimizing the extraction and separation process conditions and parameters, four homogeneous polysaccharides were purified from the zheji genus, and structural characterization was performed using various means such as NMR, IR, GC-MS, and their cartilage injury repair effect was evaluated on the transgenic zebrafish model.
Benefits of technology
The structure-uniform zhemata polysaccharide was successfully obtained, and it showed significant excellent results in cartilage injury repair, which was better than the existing drug alendronate.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses a roxburghii roxburghii polysaccharide produced in Zhejiang and application thereof in preparing a cartilage damage repairing drug. The invention extracts crude polysaccharide AZJP from the roxburghii roxburghii polysaccharide produced in Zhejiang by hot water extraction, separates AZJP by using anion exchange column DEAE-cellulose-52 and gel permeation chromatography column Sephadex G-200, obtains uniform polysaccharides AZJP-1a, AZJP-2a, AZJP-2b and AZJP-2c produced in Zhejiang, and performs UV, IR, molecular weight, monosaccharide composition, methylated sugar residues, NMR signal, triple helix structure, SEM and AFM surface microstructure and cartilage repairing activity determination. The four uniform polysaccharides have significant cartilage repairing activity on transgenic zebrafish, are superior to positive drug alendronate sodium, and have application prospects in the development of drugs for treating osteoarticular cartilage damage.
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