Construction method and application of humanized COL3A1 gene mouse model

By knocking in the human COL3A1 gene into the mouse model, a mouse model of humanized COL3A1 gene was constructed, which solved the problem of poor accuracy in type III collagen research in animal models, provided a biological model closer to humans, and improved the accuracy and effectiveness of the research.

CN119351470BActive Publication Date: 2025-03-28成都药康生物科技有限公司
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Patent Information

Application Number
CN202411965387.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-12-30
Publication Date
2025-03-28
Estimated Expiration
2044-12-30

AI Technical Summary

Technical Problem

Due to the differences in the COL3A1 amino acid sequence in animals and the corresponding proteins in humans, it is difficult for the prior art to accurately study the role of type III collagen in human diseases in animal models.

Method used

Through gene editing technology, the human COL3A1 gene was knocked into the H11 site of chromosome 11 of the fertilized egg of mouse to construct a mouse model of humanized COL3A1 gene. This model is able to stably express the human COL3A1 gene, emulating the function of human type III collagen.

Benefits of technology

This humanized COL3A1 gene mouse model provides a more human-friendly biological model for studying the structural and biological role of type III collagen, tissue fibrosis, skin, heart disease and related drug and drug device screening and safety evaluation, and improves the accuracy and effectiveness of the research.

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Abstract

The present application belongs to the field of animal models, and specifically relates to a method for constructing a humanized COL3A1 gene mouse model and its application. Based on gene editing, the present application inserts the humanized COL3A1 gene at a specific site, and the fertilized egg with the mutation can develop into a humanized COL3A1 gene mouse model, which provides a favorable model for studying the structure and biological effects of type III collagen, skin, heart, tissue fibrosis and other related diseases, as well as the evaluation of drugs, cosmetics and medical devices related to the above diseases.
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