Medicinal and edible homologous fermented product for improving constipation and preparation method of medicinal and edible homologous fermented product
By combining Chinese medicinal materials such as Cistanche, Angelica, Astragalus, and Red Date with probiotic fermentation technology, a functional food was developed, which solved the problem of constipation foods based on Chinese medicinal materials and probiotic fermentation on the market, and achieved safe and effective improvements Effects of constipation symptoms.
Patent Information
- Application Number
- CN202411730996.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-29
- Publication Date
- 2025-02-14
AI Technical Summary
There are many types of functional foods for constipation on the market, but most of them are dietary fiber as the main ingredient. There is a lack of products based on Chinese medicinal materials and probiotic fermentation, resulting in limited efficacy and side effects.
By combining Chinese herbal medicines such as Cistanche, Angelica, Astragalus, and Red Date with probiotic fermentation technology, a new functional food has been developed. Through enzymatic lysis, sterilization, fermentation, filtration and alcohol extraction, the steps of Prepare medicinal and food homologous fermented products with the effect of improving constipation.
This product has safe and effective laxative effects by regulating intestinal health and improving constipation symptoms, and has important innovative value and application prospects.
Smart Images
Figure CN119423301A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the fields of food technology and microbial fermentation technology, and in particular to a medicine-food fermented product for improving constipation and a preparation method thereof. Background Art
[0002] Constipation is a common digestive system problem, often manifested by symptoms such as reduced bowel movements, dry stools and difficulty in defecation. With the changes in modern lifestyle and dietary structure, the incidence of constipation has gradually increased, seriously affecting people's quality of life, especially chronic constipation. The patient's intestinal dysfunction and long-term constipation problems may cause more serious intestinal diseases. Currently, the methods for treating constipation mainly include drug therapy, dietary fiber supplementation and lifestyle intervention, but these methods often have limited efficacy and have certain side effects.
[0003] In recent years, functional foods based on Chinese medicinal materials have received widespread attention in improving constipation. Chinese medicinal materials not only have medicinal value, but also have therapeutic effects. In particular, Chinese medicinal materials that are both medicinal and edible can play a mild and sustained laxative effect by improving intestinal function and regulating gastrointestinal motility. Chinese medicinal materials such as Cistanche, Angelica, Astragalus, and red dates are widely used in traditional Chinese medicine to treat gastrointestinal discomfort and constipation. Among them, Cistanche has the effects of tonifying the kidney and assisting yang, moistening the intestine and promoting bowel movements; Angelica has the effects of nourishing blood and moistening the intestine, nourishing blood and activating blood circulation; Astragalus can replenish qi and raise yang, and enhance intestinal motility; red dates have the effects of nourishing the spleen and stomach and regulating qi and blood. These Chinese medicinal materials work synergistically through multiple pathways, and are expected to form a safe and effective laxative combination.
[0004] Probiotic fermentation technology has been a research hotspot in the food field in recent years. Probiotic fermentation can enhance the biological activity of food ingredients, improve their digestion and absorption rate, and increase the regulatory effect on intestinal microecology. Probiotic fermentation of Chinese medicinal materials can not only retain their original pharmacological activity, but also give them new functional properties, such as regulating the balance of intestinal flora and promoting intestinal peristalsis. Therefore, preparing functional foods based on Chinese medicinal materials that are both medicinal and edible and combined with probiotic fermentation technology for the prevention and improvement of constipation problems is an innovative approach with good application prospects.
[0005] At present, there are many kinds of functional foods for constipation on the market, but most of them use dietary fiber as the main ingredient, and there is a lack of products based on Chinese medicinal materials and probiotic fermentation. The present invention combines Chinese medicinal materials with both medicinal and edible properties, such as Cistanche, Angelica, Astragalus, and Red Dates, with probiotic fermentation technology to develop a new functional food, which aims to regulate intestinal health and improve constipation symptoms in a safe and effective way, and has important innovative value and application prospects. Summary of the invention
[0006] In order to overcome the problem that there are many kinds of functional foods for constipation on the market, but most of them use dietary fiber as the main ingredient, and there is a lack of products based on Chinese medicinal materials and probiotic fermentation.
[0007] The technical solution of the present invention is: a medicinal and edible fermented product for improving constipation, which is obtained by the following preparation method: 100g of Cistanche deserticola, 80g of Angelica sinensis, 80g of Astragalus membranaceus, and 40g of red dates, all of which are crushed into a coarse powder state using a grinder, the prepared powder is put into a fermentation tank, and 1500 ml of pure water is added to the fermentation tank at a material-liquid ratio of 1:5 to ensure that the medicinal materials and the water are fully mixed.
[0008] Preferably, the temperature of the fermenter is controlled at 45°C.
[0009] Preferably, the following food-grade enzymes are added to the fermenter at a ratio of 0.3% of the total volume of the medicinal solution: 0.3% amylase, 0.3% cellulase, 0.3% xylanase, 0.3% pectinase, and 0.3% neutral protease. The enzymatic reaction is maintained at a temperature of 45° C. for 1 hour to ensure that the various enzymes fully decompose the components in the medicinal materials.
[0010] Preferably, after the enzymatic hydrolysis reaction is completed, the liquid in the fermentation tank is sterilized with high-pressure steam for 30 minutes to ensure that the bacteria and other contaminants in the raw materials are completely killed.
[0011] Preferably, after sterilization, the drug solution is cooled to a suitable temperature and food-grade sodium carbonate is used to adjust the pH of the drug solution to 7 to maintain a neutral state suitable for probiotic fermentation.
[0012] Preferably, the probiotic powder is inoculated at a ratio of 100 g powder to 1 ton of substrate, the fermentation temperature is adjusted to 35° C., and the fermentation is started for 24 hours. During the fermentation process, the alkalinity of the fermentation tank is closely monitored to ensure that the fermentation tank automatically replenishes alkali during the fermentation process.
[0013] Preferably, after the fermentation is completed, the fermentation liquid is filtered using a double-layer gauze to remove solid residues to obtain a clarified filtrate.
[0014] Preferably, the filtered medicinal residue is placed in an extraction tank, 90% ethanol is added for alcohol extraction, and the extraction is performed at a solid-liquid ratio of 1:20 for 2 hours. After the extraction, the extract is passed through a rotary evaporator to remove the ethanol, leaving the water extract, and the water extract obtained by rotary evaporation is mixed with the previous fermentation filtrate to ensure that all active ingredients are fused.
[0015] Preferably, the combined liquid is concentrated by rotary evaporation at 60°C until it does not stick to the wall, and then transferred to a 60°C oven for drying until it becomes powder to obtain the desired fermented product.
[0016] Preferably, a method for preparing a food-medicinal fermented product for improving constipation comprises the following steps:
[0017] S11: Use a grinder to grind Chinese medicinal materials such as Cistanche deserticola, Chinese angelica, Astragalus, and red dates into a coarse powder state, and put them into a fermentation tank according to a certain proportion, and add purified water to fully mix;
[0018] S12: adding food-grade enzymes in a certain proportion at a specific temperature, and maintaining the enzymatic reaction for a period of time to ensure that the various enzymes fully decompose the components in the medicinal materials;
[0019] S13: After the enzymatic hydrolysis reaction is completed, high-pressure steam is used for sterilization to ensure that the bacteria and other contaminants in the raw materials are completely killed, and then the pH of the liquid is adjusted to a neutral state, which is suitable for probiotic fermentation;
[0020] S14: inoculating probiotic powder, adjusting the fermentation temperature, starting the fermentation process, and closely monitoring the alkalinity of the fermentation tank to ensure a smooth fermentation process;
[0021] S15: After the fermentation is completed, the fermentation liquid is filtered using a double-layer gauze to remove solid residues and obtain a clarified filtrate;
[0022] S16: extracting the drug residue obtained by filtration with alcohol, removing ethanol by rotary evaporator after the extraction, leaving water extract, and mixing the water extract obtained by rotary evaporation with the previous fermentation filtrate;
[0023] S17: The combined liquid is subjected to rotary evaporation concentration and drying treatment to obtain the desired medicine-food fermented product.
[0024] Beneficial effects of the present invention:
[0025] 1. Compared with traditional fermented food and medicine products for improving constipation, although there are many kinds of functional foods for constipation on the market, most of them use dietary fiber as the main ingredient, and there is a lack of products based on fermentation of Chinese medicinal materials and probiotics. This fermented food and medicine product for improving constipation combines Chinese medicinal materials such as Cistanche, Angelica, Astragalus, and Red Dates with probiotic fermentation technology to develop a new functional food, which aims to regulate intestinal health and improve constipation symptoms in a safe and effective way. It has important innovative value and application prospects. BRIEF DESCRIPTION OF THE DRAWINGS
[0026] Figure 1 Shown is a schematic diagram of the oligosaccharide standard curve of the present invention.
[0027] Figure 2 The schematic diagram of the effect of fermentation time on oligosaccharide yield of the present invention is shown.
[0028] Figure 3 The schematic diagram of the effect of the fermentation feed-liquid ratio on the oligosaccharide yield of the present invention is shown.
[0029] Figure 4 The schematic diagram of the effect of fermentation temperature on oligosaccharide yield of the present invention is shown.
[0030] Figure 5 The figure shows the effect of the decoction time on the yield of echinacoside of the present invention.
[0031] Figure 6 The figure shows the effect of the alcohol extract liquid ratio on the yield of echinacoside of the present invention.
[0032] Figure 7 The figure shows the effect of the present invention on the time of first black stool in constipated mice.
[0033] Figure 8 The figure shows the effect of the present invention on the number of stool particles in constipated mice.
[0034] Fig. 9 The figure shows the effect of the present invention on the small intestinal propulsion rate of constipated mice.
[0035] Fig.10 Shown is a schematic diagram of a medicinal and edible fermented product for improving constipation and a preparation method thereof according to the present invention. DETAILED DESCRIPTION
[0036] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.
[0037] See also Fig.10 The present invention provides an embodiment: a fermented food and medicine product for improving constipation, which is obtained by the following preparation method: 100g of Cistanche deserticola, 80g of Angelica sinensis, 80g of Astragalus membranaceus, and 40g of red dates. All the medicinal materials are crushed into a coarse powder using a grinder, and the prepared powder is put into a fermentation tank. According to the material-liquid ratio of 1:5, 1500 ml of pure water is added to the fermentation tank to ensure that the medicinal materials and water are fully mixed.
[0038] Preferably, the temperature of the fermenter is controlled at 45°C.
[0039] Preferably, the following food-grade enzymes are added to the fermenter at a ratio of 0.3% of the total volume of the medicinal solution: 0.3% amylase, 0.3% cellulase, 0.3% xylanase, 0.3% pectinase, and 0.3% neutral protease. The enzymatic reaction is maintained at a temperature of 45° C. for 1 hour to ensure that the various enzymes fully decompose the components in the medicinal materials.
[0040] Preferably, after the enzymatic hydrolysis reaction is completed, the liquid in the fermentation tank is sterilized with high-pressure steam for 30 minutes to ensure that the bacteria and other contaminants in the raw materials are completely killed.
[0041] Preferably, after sterilization, the drug solution is cooled to a suitable temperature and food-grade sodium carbonate is used to adjust the pH of the drug solution to 7 to maintain a neutral state suitable for probiotic fermentation.
[0042] Preferably, the probiotic powder is inoculated at a ratio of 100 g powder to 1 ton of substrate, the fermentation temperature is adjusted to 35° C., and the fermentation is started for 24 hours. During the fermentation process, the alkalinity of the fermentation tank is closely monitored to ensure that the fermentation tank automatically replenishes alkali during the fermentation process.
[0043] Preferably, after the fermentation is completed, the fermentation liquid is filtered using a double-layer gauze to remove solid residues to obtain a clarified filtrate.
[0044] Preferably, the filtered medicinal residue is placed in an extraction tank, 90% ethanol is added for alcohol extraction, and the extraction is performed at a solid-liquid ratio of 1:20 for 2 hours. After the extraction, the extract is passed through a rotary evaporator to remove the ethanol, leaving the water extract, and the water extract obtained by rotary evaporation is mixed with the previous fermentation filtrate to ensure that all active ingredients are fused.
[0045] Preferably, the combined liquid is concentrated by rotary evaporation at 60°C until it does not stick to the wall, and then transferred to a 60°C oven for drying until it becomes powder to obtain the desired fermented product.
[0046] Preferably, a method for preparing a food-medicinal fermented product for improving constipation comprises the following steps:
[0047] S11: Use a grinder to grind Chinese medicinal materials such as Cistanche deserticola, Chinese angelica, Astragalus, and red dates into a coarse powder state, and put them into a fermentation tank according to a certain proportion, and add purified water to fully mix;
[0048] S12: adding food-grade enzymes in a certain proportion at a specific temperature, and maintaining the enzymatic reaction for a period of time to ensure that the various enzymes fully decompose the components in the medicinal materials;
[0049] S13: After the enzymatic hydrolysis reaction is completed, high-pressure steam is used for sterilization to ensure that the bacteria and other contaminants in the raw materials are completely killed, and then the pH of the liquid is adjusted to a neutral state, which is suitable for probiotic fermentation;
[0050] S14: inoculating probiotic powder, adjusting the fermentation temperature, starting the fermentation process, and closely monitoring the alkalinity of the fermentation tank to ensure a smooth fermentation process;
[0051] S15: After the fermentation is completed, the fermentation liquid is filtered using a double-layer gauze to remove solid residues and obtain a clarified filtrate;
[0052] S16: extracting the drug residue obtained by filtration with alcohol, removing ethanol by rotary evaporator after the extraction, leaving water extract, and mixing the water extract obtained by rotary evaporation with the previous fermentation filtrate;
[0053] S17: The combined liquid is subjected to rotary evaporation concentration and drying treatment to obtain the desired medicine-food fermented product.
[0054] Example 1 Fermentation process optimization
[0055] Prepare the fermentation liquid, mix the Chinese medicine raw materials and water in a certain proportion, and stir evenly. Control the initial pH of the liquid to 7, and use sodium carbonate for pH adjustment. Next, the mixed liquid is enzymatically hydrolyzed. The enzymatic hydrolysis process is carried out at a temperature of 45 degrees Celsius and pH 4.8, and the reaction time is one hour. After the enzymatic hydrolysis is completed, continue to load the liquid into the fermentation tank, pass nitrogen gas to reduce the dissolved oxygen content. Set the fermentation temperature and time, and start the automatic control system. At the end of the fermentation, take a sample and filter the sample with two layers of filter cloth. The filtered sample is diluted 100 times. Filter again using a 3kD ultrafiltration membrane. Repeat the experiment three times and take the average value. Take the filtered sample, measure the sugar content by the phenol-sulfuric acid method, and measure the absorbance at 490nm.
[0056] (1) Drawing of standard curve
[0057] Oligosaccharides were used as reference substances and the standard curve was drawn using the phenol-sulfuric acid method. The absorbance of the sample solutions with different gradient concentrations at a wavelength of 490 nm was measured by ultraviolet spectrophotometry, and the linear regression equation of the total oligosaccharides in the sample was obtained as follows: A = 8.5667C + 0.062, R 2 =0.999, such as Figure 1 shown.
[0058] (2) Fermentation time optimization
[0059] The fermentation time was set to 12h, 24h, 36h, and 48h respectively. After the fermentation was completed, the sample was filtered, diluted, ultrafiltered, and the absorbance at 490nm was measured. Figure 2 As shown in the experimental results, it can be seen that the oligosaccharide content in the sample is the highest when the fermentation time is 24h.
[0060] (3) Optimization of fermentation feed-liquid ratio
[0061] The material-liquid ratio was set to 1:5, 1:8, 1:10, and 1:15 respectively. After the fermentation was completed, the sample was filtered, diluted, ultrafiltered, and the absorbance at 490nm was measured. As shown in the figure, the experimental results show that when the fermentation material-liquid ratio is 1:5, the oligosaccharide content is the highest.
[0062] (4) Fermentation temperature optimization
[0063] The fermentation temperature was set to 30°C, 35°C, 40°C, and 45°C respectively. After the fermentation was completed, the sample was filtered, diluted, and ultrafiltered, and the absorbance at 490nm was measured. Figure 4As shown in the experimental results, it can be seen that the oligosaccharide content is the highest when the fermentation temperature is 35°C.
[0064] (5) Orthogonal test
[0065] Table 1 Fermentation orthogonal test table
[0066]
[0067]
[0068] From the orthogonal test results, we can see that the maximum range is 3.532 with the yield as the index. It can be seen that the main influencing factor of the extraction process is the fermentation time, and the order of influence of each factor is A>B>C. From the mean analysis, we can see that the best extraction process is A2B1C2. That is, the fermentation time is 24h, the fermentation material-liquid ratio is 1:5, and the fermentation temperature is 35℃.
[0069] Example 2 Alcohol extraction process optimization
[0070] Optimize the alcohol extraction process of the drug residue, find the best decoction time and material-liquid ratio by adjusting the decoction time and material-liquid ratio, and increase the content of the active ingredients in the compound. In this experiment, Cistanche deserticola was used as the main drug, and its index component echinacoside was used to calculate the content of its active ingredients.
[0071] The fermented medicinal materials were filtered through a filter cloth to obtain the medicinal residues. Three groups of decoction times were prepared: 0.5 hours, 1 hour, 1.5 hours, and 2 hours; three groups of material-liquid ratios were prepared: 1:10, 1:15, and 1:20. The content of echinacoside was determined using a high performance liquid chromatograph HPLC.
[0072] (1) Optimization of alcohol extraction and decoction time
[0073] like Figure 5 As shown in the figure, the experimental results show that when the decoction time is 2h, the yield of echinacoside is the highest, but starting from 1.5h, the growth trend is slow.
[0074] (2) Optimization of alcohol extraction and decoction material-liquid ratio
[0075] like Figure 6 As shown in the experimental results, it can be seen that when the solid-liquid ratio is 1:20, the yield of echinacoside is the highest.
[0076] (3) Orthogonal test
[0077] Table 2 Alcohol extraction orthogonal test table
[0078]
[0079] From the orthogonal test results, we can see that the maximum range is 0.25 with the yield as the index. It can be seen that the main influencing factor of the extraction process is the solid-liquid ratio, and the order of influence of each factor is B>A. The mean analysis shows that the best extraction process is A3B3, that is, the decoction time is 2h and the solid-liquid ratio is 1:20.
[0080] Example 3 Animal Pharmacology Experiment
[0081] (1) Experimental animals and groups
[0082] ① Experimental animals: SPF Kunming mice, male, 7-8 weeks old, weighing 40-50g, were housed in separate cages in the clean animal experimental center of China Pharmaceutical University, with a temperature of 24±1℃ and a humidity of 50±10%. They were given free access to water and food, and the indoor light / dark cycle was 12h. Rats were given normal growth-grade feed. Animal ethics and all experimental procedures were approved by the Animal Ethics Committee of China Pharmaceutical University.
[0083] ② Grouping: After one week of adaptive feeding, the rats were randomly divided into a blank group (K), a model group (M), a positive drug group (Y), and a fermented self-prepared formula group (G) according to their body weight, with 10 rats in each group.
[0084] ③ The blank control group and the model control group were given distilled water in the same way. The test samples were given for 30 days.
[0085] (2) Reagent preparation
[0086] Preparation of prucalopride tablet solution: Use 0.9% sterile saline to prepare prucalopride tablet into a 0.0259 mg / mL solution, which is the positive drug in this experiment.
[0087] Preparation of fermented self-prepared solution: Weigh an appropriate amount of dry powder and dissolve it in 0.9% sterile saline to prepare a solution to ensure that the dosage for mice is 4.68 g / kg / d.
[0088] Preparation of ink: Accurately weigh 100g of gum arabic, add 800mL of water, boil until the solution becomes transparent, weigh 50g of activated carbon (powdered) and add it to the above solution and boil it three times. After the solution cools, add water to make up to 1000mL, store in a refrigerator at 4℃, and shake well before use.
[0089] (3) Establishment of mouse constipation model
[0090] The mice in the model control group, positive drug group and three dose groups were given loperamide 4 mg / kg BW by oral gavage for 30 consecutive days to establish a mouse constipation model. The blank control group was given an equal amount of normal saline. After two hours of loperamide administration every day, the subsequent drug administration treatment of each group was carried out.
[0091] Group G: 4.68 g / kg / d of the test sample was administered by gavage.
[0092] Blank group and model group were intragastrically administered with an equal amount of normal saline.
[0093] After the model was established, on the 30th day of the experiment, mice in each group were fasted for 16 hours (but allowed to drink water). After the fasting, loperamide was administered, and ink was administered by gavage 0.5 hours after the drug administration, and the experimental record began.
[0094] (4) Sample collection and processing
[0095] ①The time of the first black stool and the defecation situation record:
[0096] 0.5 hours after the administration of compound diphenoxylate or loperamide, the mice in the negative control group and the model control group were gavaged with ink, while the mice in each dose group were given ink containing the test samples.
[0097] The animals were housed individually and had normal drinking water and food intake. The time of the first black stool of each mouse from the time of ink injection was recorded, and the number and weight of black stools were recorded within 5 hours.
[0098] ② Determination of small intestinal propulsion rate:
[0099] After the defecation data were recorded, the animals were fasted for 16 hours but not water. After the fasting period, loperamide was given again, and ink was administered intragastrically 0.5 hours after the drug administration.
[0100] After 25 minutes of ink infusion, the mice were killed by cervical dislocation and the small intestine was separated after dissection. After separation of the mesentery, the small intestine segment from the pylorus to the ileocecal part was cut, the small intestine was gently straightened, and the total length of the small intestine and the ink push length were measured.
[0101] The calculation formula of ink advancement rate is:
[0102]
[0103] (5) Experimental data results
[0104] Fermented products have a certain effect in improving constipation.
[0105] Effects on the time to first black stool in constipated mice, such as Figure 7 shown.
[0106] Effects on the number of stool particles in constipated mice, such as Figure 8 shown.
[0107] Effects on small intestinal propulsion rate in constipated mice, such as Fig. 9 shown.
[0108] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge scope of those skilled in the art without departing from the spirit of the present invention.
Claims
1. A food-medicinal fermented product for improving constipation; characterized by: The preparation method is as follows: 100g of Cistanche deserticola, 80g of Angelica sinensis, 80g of Astragalus membranaceus, and 40g of red dates. All the medicinal materials are crushed into coarse powder using a grinder. The prepared powder is put into a fermentation tank. According to the material-liquid ratio of 1:5, 1500 ml of pure water is added to the fermentation tank to ensure that the medicinal materials and water are fully mixed.
2. The food-medicine fermented product for improving constipation according to claim 1, characterized in that: The temperature of the fermenter was controlled at 45°C.
3. The food-medicinal fermented product for improving constipation according to claim 2, characterized in that: Add the following food-grade enzymes to the fermentation tank at a ratio of 0.3% of the total volume of the medicinal solution: 0.3% amylase, 0.3% cellulase, 0.3% xylanase, 0.3% pectinase, and 0.3% neutral protease. Maintain the enzymatic reaction at 45°C for 1 hour to ensure that the various enzymes fully decompose the components in the medicinal materials.
4. The food-medicinal fermented product for improving constipation according to claim 3, characterized in that: After the enzymatic hydrolysis reaction is completed, the liquid in the fermentation tank is sterilized with high-pressure steam for 30 minutes to ensure that the bacteria and other contaminants in the raw materials are completely killed.
5. The food-medicine fermented product for improving constipation according to claim 4, characterized in that: After sterilization, wait for the liquid medicine to cool to a suitable temperature, and use food-grade sodium carbonate to adjust the pH of the liquid medicine to 7 to maintain a neutral state, which is suitable for probiotic fermentation.
6. The food-medicine fermented product for improving constipation according to claim 5, characterized in that: Probiotic powder was inoculated according to the ratio of 100g powder to 1 ton of substrate, the fermentation temperature was adjusted to 35°C, and fermentation was started for 24 hours. During the fermentation process, the alkalinity of the fermentation tank was closely monitored to ensure that the fermentation tank automatically replenished alkali during the fermentation process.
7. The food-medicine fermented product for improving constipation according to claim 6, characterized in that: After fermentation, the fermentation liquid was filtered using double-layer gauze to remove solid residues and obtain a clear filtrate.
8. The food-medicine fermented product for improving constipation according to claim 7, characterized in that: The filtered medicinal residue is placed in an extraction tank, and 90% ethanol is added for alcohol extraction. The extraction is carried out at a solid-liquid ratio of 1:20, and the extraction time is 2 hours. After the extraction is completed, the extract is passed through a rotary evaporator to remove the ethanol, leaving the water extract. The water extract obtained by rotary evaporation is mixed with the previous fermentation filtrate to ensure that all active ingredients are integrated.
9. The food-medicine fermented product for improving constipation according to claim 8, characterized in that: The combined liquid was concentrated by rotary evaporation at 60°C until it was no longer sticky to the wall, and then dried in an oven at 60°C until it became powder to obtain the desired fermented product.
10. A food-and-drug fermented product for improving constipation according to claims 1-9, and a method for preparing a food-and-drug fermented product for improving constipation, characterized in that: The following steps are involved: S11: Use a grinder to grind Chinese medicinal materials such as Cistanche deserticola, Chinese angelica, Astragalus, and red dates into a coarse powder state, and put them into a fermentation tank according to a certain proportion, and add purified water to fully mix; S12: adding food-grade enzymes in a certain proportion at a specific temperature, and maintaining the enzymatic reaction for a period of time to ensure that the various enzymes fully decompose the components in the medicinal materials; S13: After the enzymatic hydrolysis reaction is completed, high-pressure steam is used for sterilization to ensure that the bacteria and other contaminants in the raw materials are completely killed, and then the pH of the liquid is adjusted to a neutral state, which is suitable for probiotic fermentation; S14: inoculating probiotic powder, adjusting the fermentation temperature, starting the fermentation process, and closely monitoring the alkalinity of the fermentation tank to ensure a smooth fermentation process; S15: After the fermentation is completed, the fermentation liquid is filtered using a double-layer gauze to remove solid residues and obtain a clarified filtrate; S16: extracting the drug residue obtained by filtration with alcohol, removing ethanol by rotary evaporator after the extraction, leaving water extract, and mixing the water extract obtained by rotary evaporation with the previous fermentation filtrate; S17: The combined liquid is subjected to rotary evaporation concentration and drying treatment to obtain the desired medicine-food fermented product.