Preparation method of stable nano platinum sol

Abstract

The invention discloses a preparation method of stable nano platinum sol, which comprises the following steps: mixing a potassium chloroplatinate solution and a stabilizer solution according to a certain proportion to obtain a mixed solution A; adding a reducing agent solution into the obtained mixed solution A to obtain a mixed solution B, and carrying out a reduction reaction; after the reaction is finished, nano platinum sol is obtained, the obtained nano platinum sol is preserved, and the particle size, the particle size range, the morphology, the dispersity and the stability of the nano platinum sol are regulated and controlled by selecting different types of reducing agents and controlling the reaction temperature and the reaction time; the nano platinum sol is prepared through a chemical method, the preparation method is simple, and large-scale industrial production is easy.

Description

Technical Field

[0001] The invention belongs to the technical field of platinum nano material preparation, and particularly relates to a method for preparing a stable nano platinum sol. Background Art

[0002] Nanometal materials have a series of excellent physical and chemical properties due to their small particle size and large specific surface area. These properties make nanoplatinum have a wide range of application potentials in the fields of biology and medicine, including catalysis, antibacterial, anticancer and biotherapy. However, these characteristics will also cause its behavior in the body to be different from that of platinum powder with larger particle size, thus affecting its biocompatibility; the size of platinum powder particles will affect its distribution and cellular uptake in the body. Smaller platinum powder particles are more easily taken up by cells because they can pass through the cell membrane more easily. This increased cellular uptake may lead to an increase in intracellular platinum concentration, thereby increasing cytotoxicity; in addition, smaller platinum powder particles have higher surface activity, which may lead to stronger oxidative stress and inflammatory responses, thereby affecting its biocompatibility. The particle size of platinum powder has a significant effect on its biocompatibility; when the particle size of platinum nanoparticles is 6-8nm, they show good biocompatibility and do not increase cytotoxicity;

[0003] At present, the preparation methods of nano-platinum sol can be roughly divided into chemical methods and physical methods. The existing chemical method has some challenges in preparing nano-platinum sol. Specifically, this method cannot control the particle size of the synthesized nano-platinum sol within the range of 6-8nm, and cannot prepare nano-platinum sol with high biocompatibility and low cytotoxicity. Summary of the invention

[0004] The main purpose of the present invention is to provide a method for preparing a stable nano-platinum sol, which solves the problems of uncontrollable particle size, low biocompatibility, high cytotoxicity, different morphology, poor stability, complex preparation process and difficulty in large-scale industrial production in the existing preparation methods.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] A method for preparing a stable nano-platinum sol comprises the following steps:

[0007] S1, mixing a potassium chloroplatinate solution and a stabilizer solution in a certain proportion to obtain a mixed solution A;

[0008] S2, adding a reducing agent solution to the mixed solution A obtained in step S1 to obtain a mixed solution B and performing a reduction reaction;

[0009] S3, after the reaction is completed, nano-platinum sol is obtained, and the obtained nano-platinum sol is stored.

[0010] Furthermore, the stabilizer is one or more of polyvinyl pyrrolidone, chitosan, sodium alginate, α-cyclodextrin, β-cyclodextrin, and hydroxypropyl β-cyclodextrin.

[0011] Furthermore, the concentration of the stabilizer solution is 10 g / L.

[0012] Furthermore, the concentration of the potassium chloroplatinate solution is 10 mM.

[0013] Furthermore, the reducing agent is one of sodium borohydride and ascorbic acid, and the concentration of the reducing agent is 10 g / L.

[0014] Furthermore, the molar ratio of potassium chloroplatinate to the reducing agent in the mixed solution B is 1:(1-10).

[0015] Furthermore, the molar ratio of potassium chloroplatinate to the stabilizer in the mixed solution B is 1:(1-100).

[0016] Furthermore, in step S2, if the reducing agent is sodium borohydride, the reaction temperature is room temperature and the reaction time is 30-90 min; if the reducing agent is ascorbic acid, the reaction temperature is 50-100° C. and the reaction time is 8-30 min.

[0017] Furthermore, the storage temperature in step S3 is 2-4°C.

[0018] In summary, this application has the following beneficial effects:

[0019] 1. The method of the present application regulates the particle size, particle size range, morphology, dispersibility and stability of the nano-platinum sol by selecting different types of reducing agents, controlling the reaction temperature and reaction time;

[0020] 2. The method of the present application prepares nano-platinum sol by chemical method, which is simple and easy to be mass-produced industrially. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a transmission electron microscope image of the nano-platinum particles obtained in Example 1. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0023] Example 1

[0024] Prepare potassium chloroplatinate solution: weigh 48.6 mg potassium chloroplatinate and dissolve it in 10 mL water; prepare reducing agent solution: weigh 1 g sodium borohydride and dissolve it in 10 mL water; prepare stabilizer solution: weigh 0.1 g polyvinyl pyrrolidone and dissolve it in 10 mL water; use a pipette to take 1 mL of the prepared potassium chloroplatinate solution, 2 mL of polyvinyl pyrrolidone solution and 14 mL of water and add them to a beaker, use a syringe to take 3 mL of sodium borohydride solution and add it to the beaker, the reaction temperature is room temperature, the reaction time is 30 minutes, and after the reaction is completed, store it at 2-4°C. The transmission electron microscope image of the obtained nano-platinum particles is as follows Figure 1 shown.

[0025] Example 2

[0026] Prepare potassium chloroplatinate solution: weigh 48.6 mg of potassium chloroplatinate and dissolve it in 10 mL of water; prepare reducing agent solution: weigh 0.1 g of sodium borohydride and dissolve it in 10 mL of water; prepare stabilizer solution: weigh 0.3 g of polyvinyl pyrrolidone and dissolve it in 30 mL of water; use a pipette to take 10 mL of the prepared potassium chloroplatinate solution, 20 mL of polyvinyl pyrrolidone solution and 8 mL of water and add them to a beaker, use a syringe to take 3 mL of sodium borohydride solution and add it to the beaker, the reaction temperature is room temperature, the reaction time is 50 minutes, and after the reaction is completed, store it at 2-4°C.

[0027] Example 3

[0028] Prepare potassium chloroplatinate solution: weigh 48.6 mg of potassium chloroplatinate and dissolve it in 10 mL of water; prepare reducing agent solution: weigh 0.1 g of sodium borohydride and dissolve it in 10 mL of water; prepare stabilizer solution: weigh 0.3 g of sodium alginate and dissolve it in 30 mL of water; use a pipette to take 6.5 mL of the prepared potassium chloroplatinate solution and 15 mL of sodium alginate solution and add them to a flask, use a syringe to take 5 mL of sodium borohydride solution and add it to a beaker, the reaction temperature is room temperature, the reaction time is 60 minutes, and after the reaction is completed, store it at 2-4°C.

[0029] Example 4

[0030] Prepare potassium chloroplatinate solution: weigh 48.6 mg of potassium chloroplatinate and dissolve it in 10 mL of water; prepare reducing agent solution: weigh 0.1 g of ascorbic acid and dissolve it in 10 mL of water; prepare stabilizer solution: weigh 0.5 g of chitosan and dissolve it in 50 mL of water; use a pipette to take 4 mL of the prepared potassium chloroplatinate solution and 44 mL of chitosan solution and add them into a three-necked flask, heat it to 100°C, use a syringe to take 2 mL of ascorbic acid and quickly inject it into the flask, the reaction time is 30 minutes, and after the reaction is completed, store it at 2-4°C.

[0031] Example 5

[0032] Prepare potassium chloroplatinate solution: weigh 48.6 mg of potassium chloroplatinate and dissolve it in 10 mL of water; prepare reducing agent solution: weigh 0.1 g of sodium borohydride and dissolve it in 10 mL of water; prepare stabilizer solution: weigh 0.1 g of α-cyclodextrin and dissolve it in 10 mL of water; use a pipette to take 1 mL of the prepared potassium chloroplatinate solution, 2 mL of α-cyclodextrin and 14 mL of water and add them into a beaker, use a syringe to take 3 mL of sodium borohydride solution and quickly inject it into the beaker, the reaction temperature is room temperature, the reaction time is 70 minutes, and after the reaction is completed, store it at 2-4°C.

[0033] Example 6

[0034] Prepare potassium chloroplatinate solution: weigh 48.6 mg of potassium chloroplatinate and dissolve it in 10 mL of water; prepare reducing agent solution: weigh 0.1 g of sodium borohydride and dissolve it in 10 mL of water; prepare stabilizer solution: weigh 0.1 g of β-cyclodextrin and dissolve it in 10 mL of water; use a pipette to take 1 mL of the prepared potassium chloroplatinate solution, 2 mL of β-cyclodextrin and 14 mL of water and add them into a beaker, use a syringe to take 3 mL of sodium borohydride solution and quickly inject it into the beaker, the reaction temperature is room temperature, the reaction time is 80 minutes, and after the reaction is completed, store it at 2-4°C.

[0035] Example 7

[0036] Prepare potassium chloroplatinate solution: weigh 48.6 mg of potassium chloroplatinate and dissolve it in 10 mL of water; prepare reducing agent solution: weigh 0.1 g of sodium borohydride and dissolve it in 10 mL of water; prepare stabilizer solution: weigh 0.1 g of hydroxypropyl β-cyclodextrin and dissolve it in 10 mL of water; use a pipette to take 1 mL of the prepared potassium chloroplatinate solution, 2 mL of hydroxypropyl β-cyclodextrin and 14 mL of water and add them into a beaker, use a syringe to take 3 mL of sodium borohydride solution and quickly inject it into the beaker, the reaction temperature is room temperature, the reaction time is 90 minutes, and after the reaction is completed, store it at 2-4°C.

[0037] Example 8

[0038] Prepare potassium chloroplatinate solution: weigh 48.6 mg of potassium chloroplatinate and dissolve it in 10 mL of water; prepare reducing agent solution: weigh 0.1 g of ascorbic acid and dissolve it in 10 mL of water; prepare stabilizer solution: weigh 0.5 g of polyvinyl pyrrolidone and dissolve it in 50 mL of water; use a pipette to take 4 mL of the prepared potassium chloroplatinate solution and 44 mL of polyvinyl pyrrolidone and add them into a three-necked flask, heat it to 70°C, use a syringe to take 3 mL of ascorbic acid and quickly inject it into the flask, the reaction time is 10 minutes, and after the reaction is completed, store it at 2-4°C.

[0039] The morphology, particle size distribution range, and ultraviolet absorption peak of the products of Examples 1-8 were tested, and the test results are shown in Table 1, wherein the morphology and particle size distribution range of the products were measured by transmission electron microscopy, and the ultraviolet absorption peak was measured by UV-Vis spectroscopy.

[0040] Table 1

[0041]

[0042] The nano platinum sol prepared by the present invention does not contain (PtCl6) 2- The exclusive characteristic peak indicates that potassium chloroplatinate is completely reacted during the preparation process. Transmission electron microscopy shows that the nano-platinum particles are spherical with an average particle size of 7nm. The particle size is mainly distributed in the range of 6-8nm. The particle size is controllable, the stability is good, and the dispersion is good.

[0043] It should be understood that the preferred specific embodiments of the present invention can be modified and varied by ordinary technicians in the field without creative work. Therefore, any technical solution that can be obtained by technicians in the field of technology through logical analysis, reasoning or limited experiments based on the concept of the present invention on the basis of the prior art should be within the scope of protection determined by the claims.

Claims

1. A method for preparing a stable nano-platinum sol, characterized in that: The steps include: S1, mixing a potassium chloroplatinate solution and a stabilizer solution in a certain proportion to obtain a mixed solution A; S2, adding a reducing agent solution to the mixed solution A obtained in step S1 to obtain a mixed solution B and performing a reduction reaction; S3, after the reaction is completed, nano-platinum sol is obtained, and the obtained nano-platinum sol is stored.

2. The method for preparing a stable nano-platinum sol according to claim 1, characterized in that: The stabilizer is one or more of polyvinyl pyrrolidone, chitosan, sodium alginate, α-cyclodextrin, β-cyclodextrin, and hydroxypropyl β-cyclodextrin.

3. The method for preparing a stable nano-platinum sol according to claim 2, characterized in that: The concentration of the stabilizer solution is 10 g / L.

4. The method for preparing a stable nano-platinum sol according to claim 1, characterized in that: The concentration of the potassium chloroplatinate solution is 10 mM.

5. The method for preparing a stable nano-platinum sol according to claim 1, characterized in that: The reducing agent is one of sodium borohydride and ascorbic acid, and the reducing agent concentration is 10 g / L.

6. The method for preparing a stable nano-platinum sol according to claim 1, characterized in that: The molar ratio of potassium chloroplatinate to the reducing agent in the mixed solution B is 1:(1-10).

7. The method for preparing a stable nano-platinum sol according to claim 1, characterized in that: The molar ratio of potassium chloroplatinate to the stabilizer in the mixed solution B is 1:(1-100).

8. The method for preparing a stable nano-platinum sol according to claim 1, characterized in that: In step S2, if the reducing agent is sodium borohydride, the reaction temperature is room temperature and the reaction time is 30-90 min; if the reducing agent is ascorbic acid, the reaction temperature is 50-100° C. and the reaction time is 8-30 min.

9. The method for preparing a stable nano-platinum sol according to claim 1, characterized in that: The storage temperature in step S3 is 2-4°C.

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