Spodoptera frugiperda attractant and application thereof

Abstract

The invention belongs to the field of insect attractants, and particularly relates to a spodoptera frugiperda attractant and application thereof. The attractant is prepared from the following components: 5-methyl-1, 3-dihydrobenzimidazole-2-ketone, isopropyl alcohol, phenylacetaldehyde and phenethyl alcohol. The sex attractant realizes simultaneous trapping and killing of male and female adults, so the population density can be greatly reduced, the mating rate is reduced, the damage of pests to crops is reduced, and the problems of monitoring and early warning hysteresis quality and high miscellaneous moth rate of a sex attractant core for the male adults of the spodoptera frugiperda in actual production are solved.

Description

Technical Field

[0001] The invention belongs to the field of insect attractants, and particularly relates to a fall armyworm attractant and application thereof. Background Art

[0002] Spodoptera frugiperda (JESmith), also known as the fall armyworm, is a notorious polyphagous invasive pest native to tropical and subtropical regions of the Americas. The global population of S. frugiperda can be divided into two phenotypically similar but genetically distinct subpopulations or strains: the rice strain (RS) and the maize strain (CS). The insect feeds on 353 plant species from 76 families. In January 2019, the fall armyworm invaded China and has since mainly fed on maize throughout the country. It quickly spread to most parts of China in a short period of time, feeding not only on maize but also on other crops such as rice, sugarcane, sorghum and millet. This astonishing spread can be attributed to the pest's rapid migration ability, high reproductive potential, polyphagous nature and difficulty in control. S. frugiperda adults need to supplement nutrition to complete the mating and egg-laying process in order to achieve the purpose of reproduction. Although S. frugiperda feeds on grass heads and corn secretions in the field and is rarely found on flowers, research results have shown that these moths can be attracted by the smell of other food sources associated with flowers. Insect behavior regulation technology is a green control technology developed using the behavioral responses of insects to visual, olfactory and other stimuli. Olfactory behavior regulation technology uses various odor compounds from insects, plants, etc. to specifically regulate the behavior of target pests, and slowly releases artificially synthesized information compounds into the field with a releaser to attract pests to feed, lay eggs, and mate, directly trap and kill adult insects or interfere with pest mating, and reduce the population of offspring of target pests, thereby achieving the purpose of green pest control. At present, chemical pesticides are mainly used in production to control fall armyworms. However, the long-term and large-scale use of chemical pesticides has caused fall armyworms to develop resistance, resulting in low pesticide utilization efficiency, waste of pesticides, and inability to completely control them. Insect sex pheromones are trace volatile chemicals secreted by insects when they reach sexual maturity and can attract individuals of the opposite sex of the same species for mating. They are species-specific and are therefore widely used in the monitoring and control of pests. In recent years, my country has made a series of progress in the industrial synthesis, purification, and stabilization of insect sex pheromones, the improvement and perfection of the slow-release production process, the development and research of various dry traps, the research and development of intelligent sex pheromone detection and reporting technologies, and the demonstration of practical sex pheromone technology for field sex pheromones for various pests, which has greatly promoted the monitoring, forecasting, and green control of major pests.

[0003] The monitoring and prevention and control area of ​​fall armyworm has a large span, long time and large temperature difference. It is necessary to adopt corresponding attractants for different environments. Different strategies should be adopted in different regions according to its migratory nature, tenderness, sex pheromone release and courtship characteristics. Henan Province is located at 31°23'-36°22' north latitude and 110°21' to 116°39' east longitude. It is the main production area of ​​summer corn in the Huanghuaihai area, the key prevention area for the world's migratory pest fall armyworm, and the migration transition area. In field production, the attractants of the widely used male adult attractants have a lag in monitoring and early warning. Summary of the invention

[0004] In order to solve the above problems, the present invention provides a fall armyworm attractant, comprising 5-methyl-1,3-dihydrobenzimidazol-2-one, isopropanol, phenylacetaldehyde, phenylethanol, a solvent and a carrier.

[0005] Furthermore, the solvent is edible oil.

[0006] Furthermore, the carrier is a capillary.

[0007] The present invention also provides a method for preparing the above-mentioned fall armyworm attractant, which is characterized by comprising the following steps:

[0008] (1) Dilution: Using edible oil as solvent, dilute 5-methyl-1,3-dihydrobenzimidazol-2-one to 5 ng / μL, dilute isopropanol to 10 ng / μL, dilute phenylacetaldehyde to 2 ng / μL, and dilute phenylethanol to 3 ng / μL;

[0009] (2) mixing, mixing the diluted 5-methyl-1,3-dihydrobenzimidazol-2-one, isopropyl alcohol, phenylacetaldehyde and phenylethanol in a volume ratio of 5:10:2:3 to prepare a filler;

[0010] (3) Loading: Use the capillary as a carrier to load the filler.

[0011] The present invention also provides application of the fall armyworm attractant in preventing and controlling insect pests.

[0012] Furthermore, the insect pests include insect pests caused by the fall armyworm.

[0013] The present invention has the following beneficial effects:

[0014] The present invention achieves the simultaneous trapping and killing of male and female adults, reduces the mating rate, and solves the problems of delayed monitoring and early warning of sex attractant cores of male adults of fall armyworm and a high rate of mixed moths in actual production. DETAILED DESCRIPTION

[0015] Now, various exemplary embodiments of the present invention are described in detail. The methods in the examples are conventional methods unless otherwise specified, and the reagents used are conventional commercially available reagents or reagents prepared by conventional methods unless otherwise specified. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, features and embodiments of the present invention.

[0016] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0017] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0018] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0019] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0020] Example 1 Preparation of attractant

[0021] The present invention uses volatile 5-methyl-1,3-dihydrobenzimidazol-2-one (CAS: 5400-75-9), isopropanol (CAS: 67-63-0), phenylacetaldehyde (CAS: 122-78-1) and phenylethanol (CAS: 60-12-8) to attract male and female adults of fall armyworm in the field. The attractant formula is shown in Table 1.

[0022] Table 1 Attractant formula

[0023]

[0024]

[0025] The preparation method of the attractant comprises the following steps:

[0026] 1. Dilution: Use cooking oil to dilute 5-methyl-1,3-dihydrobenzimidazol-2-one, isopropanol, phenylacetaldehyde and phenylethanol to 5 ng / μL, 10 ng / μL, 2 ng / μL and 3 ng / μL respectively.

[0027] 2. Mix the diluted 5-methyl-1,3-dihydrobenzimidazol-2-one, isopropanol, phenylacetaldehyde and phenylethanol in a volume ratio of 5:10:2:3 (1.25 ng / μL: 5 ng / μL: 0.2 ng / μL and 0.45 ng / μL).

[0028] 3. Carrier: The capillary tube is used as a carrier for the filler (5-methyl-1,3-dihydrobenzimidazol-2-one + isopropanol + phenylacetaldehyde + phenylethanol).

[0029] Example 2 Verification of the performance of the above attractant

[0030] A bait validation experiment was conducted in a maize field (from the tassel stage to the tassel stage) in Pingyuan New District, Xinxiang City, Henan Province, China (35°18′N, 113°52′E, altitude: 78-83m) from July 25 to September 25, 2024. A single bait was loaded into the top baffle of a bucket device (Beijing Zhongjie Sifang Biotechnology Co., Ltd., Beijing, China). The bucket device was hung on an iron rod 20 cm above the maize seedlings, with an interval of 20 meters, and a randomized complete block design was used with 6 replicates. The number of adults captured by each trap was recorded, and the trap was emptied every 2 days. The bucket device without bait was used as a control.

[0031] A total of 168 male and female adults were trapped in this study, and the difference compared with the control (0 in the empty bucket-type equipment without bait core) reached an extremely significant level (p<0.01). The increase in the number of male and female adults lured will lead to a decrease in the mating rate, thereby reducing the reproduction rate and ultimately reducing the degree of damage in the field.

[0032] Example 3 Comparative Test

[0033] A core bait validation experiment was conducted in a maize field (from the tassel stage to the tassel stage) in Pingyuan New District, Xinxiang City, Henan Province, China (35°18′N, 113°52′E, altitude: 78-83m) from July 28 to September 18, 2024. The core baits (5-methyl-1,3-dihydrobenzimidazol-2-one only, 5-methyl-1,3-dihydrobenzimidazol-2-one + isopropanol mixture, 5-methyl-1,3-dihydrobenzimidazol-2-one + isopropanol + phenylacetaldehyde mixture) and the control core bait (Zhongjie Sifang) were loaded into the top partition of a bucket device (Beijing Zhongjie Sifang Biotechnology Co., Ltd., Beijing, China). The bucket device was hung on an iron pole 20 cm above the maize seedlings, with an interval of 20 meters, and a randomized complete block design was used with 4 replicates. The number of adults captured by each trap was recorded, and the trap was emptied every 2 days. The Zhongjie Sifang bait core was used as a control.

[0034] A total of 168 male and female adults were trapped in this study. The differences were extremely significant (p<0.01) compared with the controls of 5-methyl-1,3-dihydrobenzimidazol-2-one only (1), 5-methyl-1,3-dihydrobenzimidazol-2-one + isopropanol mixed bait (2) and 5-methyl-1,3-dihydrobenzimidazol-2-one + isopropanol + phenylacetaldehyde mixture (3). The increase in the number of male and female adults lured will lead to a decrease in the mating rate, thereby reducing the reproduction rate and ultimately reducing the degree of damage in the field.

[0035] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A fall armyworm attractant, characterized in that: The invention comprises 5-methyl-1,3-dihydrobenzimidazol-2-one, isopropyl alcohol, phenylacetaldehyde, phenylethanol, a solvent and a carrier.

2. The fall armyworm attractant according to claim 1, characterized in that The solvent is edible oil.

3. The fall armyworm attractant according to claim 1, characterized in that The carrier is a capillary tube.

4. A method for preparing the fall armyworm attractant as claimed in claim 1, characterized in that: The following steps are involved: (1) Dilution: Using edible oil as solvent, dilute 5-methyl-1,3-dihydrobenzimidazol-2-one to 5 ng / μL, dilute isopropanol to 10 ng / μL, dilute phenylacetaldehyde to 2 ng / μL, and dilute phenylethanol to 3 ng / μL; (2) mixing, mixing the diluted 5-methyl-1,3-dihydrobenzimidazol-2-one, isopropyl alcohol, phenylacetaldehyde and phenylethanol in a volume ratio of 5:10:2:3 to prepare a filler; (3) Loading: Use the capillary as a carrier to load the filler.

5. Use of the fall armyworm attractant as described in any one of claims 1 to 3 in controlling insect pests.

6. The use according to claim 5, characterized in that: The insect pests include insect pests caused by the fall armyworm.

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