Fish scale marking method

Abstract

The invention relates to the technical field of aquatic animal group marking, and discloses a fish scale marking method which comprises the following steps: (1) ablating scales of a to-be-marked fish body by using laser to form a marked pattern; (2) after the ablation operation is executed, dipping a medicament to wipe the marked pattern; the medicament comprises the following components in percentage by mass: 1-3% of a coloring agent, 5-10% of chitosan, 1-3% of an antibacterial agent and the balance of a phosphate buffer solution; and (3) standing to finish marking. According to the method, laser ablation and special medicament treatment are combined, a lasting mark can be formed on the surface of the fish body, the method has the remarkable advantages of being high in mark retention rate, low in operation cost and small in influence on the fish body, and a new technical choice is provided for fishery management and fish resource protection.

Description

Technical Field

[0001] The invention relates to the technical field of aquatic animal group marking, in particular to a fish scale marking method. Background Art

[0002] In fishery management and ecological protection, tagging technology plays a vital role in the process of fish enhancement and release. By tagging fish, their migration paths, behavior patterns and survival rates can be effectively tracked, providing key data for evaluating the effectiveness of release. Tagging will also help understand the dynamics and ecological relationships of fish populations, and provide a reference for ensuring the sustainable management of fishery resources. In addition, tagging technology also plays an important role in controlling invasive species and protecting local ecosystems. In summary, tagging technology is an indispensable tool for evaluating the effects of value-added release and optimizing fishery management.

[0003] At present, the methods of marking fish include fin clipping, fluorescence, tagging, otolith staining, visual implant elastomer (VIE), passive integrated transponder (PIT) and other marking methods. However, although these methods are widely used in practical applications, they still face some challenges, such as the shedding of tags, potential damage to the fish body, and difficulty in identifying or detecting tags. These problems may lead to deviations in the evaluation of the release effect, thus affecting subsequent management and protection work.

[0004] Compared with traditional methods, fish scale marking technology provides an innovative and effective solution. Fish scale marking technology uses high-energy laser beams to process the surface of the material at the micron level, and forms permanent marks on the surface of the scales through local high temperature or photochemical reactions. The main components of fish scales include collagen, apatite, calcium carbonate and stratum corneum. Laser marking changes the physical and chemical structure of the surface layer of the scales to form visible marks in the scales, such as text, patterns or numbers. Since fish scales do not fall off by themselves during normal growth and life, laser marking can produce contrasting and lasting marks without destroying the scale structure. These marks will not fall off or disappear due to the fish's swimming, foraging or living in different water qualities.

[0005] However, increasing the degree of laser ablation to make its application on fish scales more durable will cause potential damage to the fish body, increase the risk of infection or foreign body rejection, and more seriously affect the fish's swimming ability, foraging behavior or reproductive ability. Summary of the invention

[0006] In order to solve the above-mentioned technical problems, the present invention provides a fish scale marking method, which can form a permanent mark on the surface of the fish body by combining laser ablation and special chemical treatment, thereby improving the mark retention rate while causing less damage to the fish body. Therefore, it is more efficient, accurate, and has lower operating costs.

[0007] The purpose of the present invention is achieved through the following technical solutions: The present invention provides a fish scale marking method, comprising the following steps: (1) The scales of the fish to be marked are ablated using a laser to form a marking pattern; (2) After the ablation operation is performed, the marking pattern is wiped with a reagent; the reagent includes 1 to 3% dye, 5 to 10% chitosan and 1 to 3% antibacterial agent by mass, and the rest is phosphate buffer; (3) After that, let it stand and complete the marking.

[0008] Before marking, the fish scales are first accurately measured. The width and length of the fish scales of randomly selected fish bodies are measured using an optical microscope or stereoscope. In specific operations, the scales on the dorsal and ventral sides of the fish body trunk are used as measurement objects to ensure the scientific nature of the marking position and the accuracy of the data. Before laser ablation, the fish body is anesthetized with anesthetics, and the head and caudal peduncle are elastically fixed using mechanical equipment to ensure that the fish body remains stable during the marking process. Subsequently, a soft cloth or sponge with good water absorption is used to gently wipe the marked area to remove surface mucus to avoid affecting the marking effect.

[0009] Professional laser emission equipment is used, and the ablation operation is performed by computer program control. By writing a computer program, the output power, wavelength, time, frequency and moving distance of the laser are precisely controlled to ensure the uniformity and accuracy of the marking. After the ablation operation is completed, the marked area needs to be processed. Use a soft cloth or sponge dipped in a special agent to gently wipe the ablation area. After wiping, let it stand to ensure that the agent fully penetrates the ablation point, thereby enhancing the visibility and durability of the mark.

[0010] Preferably, the fish body to be marked is pre-treated with anesthesia before laser ablation.

[0011] Preferably, the anesthesia pretreatment is: anesthetizing the fish to be marked and wiping the marked area to remove mucus on the body surface.

[0012] Preferably, the ablation depth is 5 to 15 μm.

[0013] As a preference, the average length L of the scales is a and the average measured width W a Calculate the area of ​​a single scale S = L a *W a , then the ablation area on a single scale does not exceed 0.3% of S and is not less than 0.01% of S. More preferably, the ablation area on a single scale is 0.03-0.25% of S.

[0014] Preferably, the ablation area on a single scale is 500 to 8000 μm 2 More preferably, the ablation area on a single scale is 1500 to 8000 μm 2 .

[0015] Preferably, a circular mark with a diameter of 25 to 100 μm is formed on a single scale.

[0016] Preferably, the marking graphics are distributed near the dorsal side and / or near the ventral side of the fish body trunk.

[0017] Preferably, the dye comprises Alizarin Red S (molecule formula C 14 H 7 NaO 7 S), Orange G (Orange G, molecular formula C 6 H 9 NS) or aniline blue (Aniline Blue, molecular formula C 32 H 25 N 3 O 9 S 3 Na 2 ) one or more of the following.

[0018] The dyes used react with the collagen and calcification in the scales to create a durable, contrasting mark.

[0019] Preferably, chitosan is a substance that promotes the healing of epidermal cells. Its chemical name is β-(1-4)-2-amino-2-deoxy-D-glucose, and its molecular formula is (C 6 H 11 NO 4 )n.

[0020] Chitosan can promote cell regeneration and repair of scales and reduce the damage to scales caused by laser marking.

[0021] Preferably, the antibacterial agent is povidone-iodine (PVP-I), whose chemical formula is (C 6 H 9 NO)n·xI.

[0022] Antimicrobial agents are effective in preventing the risk of infection after marking and keeping the scales healthy.

[0023] Preferably, the phosphate buffer is a PBS buffer, comprising sodium dihydrogen phosphate (NaH 2 PO 4 ), disodium hydrogen phosphate (Na 2 HPO4 ), sodium chloride (NaCl) and water, pH value is 7.0 ~ 7.4.

[0024] Phosphate buffer can ensure the stability of the drug in the water environment and is non-irritating to fish.

[0025] Preferably, the ablation operation is performed after an interval of 5 to 10 seconds before the agent is wiped.

[0026] Preferably, the marking pattern is wiped with the reagent and then left to stand for 5 to 10 seconds to complete the marking.

[0027] Compared with the prior art, the present invention has the following beneficial effects: (1) Through the steps of fish scale size measurement, laser ablation and special agent treatment, a permanent mark can be formed on the surface of the fish body; (2) It has the significant advantages of high tag retention rate, low operating cost and little impact on fish bodies, providing a new technical option for fishery management and fish resource protection; (3) This method does not require complex DNA or elemental analysis or additional labeling to distinguish released populations, effectively overcoming the limitations of traditional labeling methods. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is an optical microscope photograph of a single large yellow croaker scale.

[0029] Figure 2 This is an optical microscope photograph of the marked large yellow croaker scales (the black dots are marked graphics).

[0030] Figure 3 This is an optical microscope photograph of the scales of the striped sea bream after marking (the black dots are marked graphics). DETAILED DESCRIPTION

[0031] The technical solution of the present invention is described below with specific embodiments, but the protection scope of the present invention is not limited thereto.

[0032] The fish scale marking method comprises the following steps: (1) The fish to be marked is anesthetized, and the area to be marked is wiped to remove mucus on the body surface; the ablation area on a single scale is determined based on the measured scale size data of the fish to be marked, the fish to be marked are fixed one by one under the laser transmitter, and the fish are presented in the working area. Computer programming is performed based on data such as ablation depth and ablation area, and the laser transmitter ablates the scales of the fish to be marked according to the programmed parameters. The scales of the fish to be marked are ablated using laser to form a marking pattern.

[0033] (2) After the ablation operation is performed, the marking pattern is wiped with a reagent at an interval of 5 to 10 seconds; the reagent includes 1 to 3% dye, 5 to 10% chitosan and 1 to 3% antibacterial agent by mass, and the rest is phosphate buffer.

[0034] (3) After wiping, let it stand for 5 to 10 seconds to complete the marking.

[0035] In a specific embodiment of the present invention, according to the average measured length L of the scales a and the average measured width W a Calculate the area of ​​a single scale S = L a *W a , the ablation area on a single scale does not exceed 0.3% of S.

[0036] In a specific embodiment of the present invention, the depth of ablation is 5-15 μm.

[0037] In a specific embodiment of the present invention, a circular mark with a diameter of 25 to 100 μm is formed on a single scale, and the ablation area on a single scale is 500 to 8000 μm. 2 .

[0038] In a specific embodiment of the present invention, the marking patterns are distributed near the dorsal side and / or near the ventral side of the fish body trunk.

[0039] In a specific embodiment of the present invention, when dipping the agent to wipe the marking pattern, the step is repeated several times until the color of the dye completely covers the marking pattern.

[0040] In a specific embodiment of the present invention, the dye comprises Alizarin Red S (molecule formula C 14 H 7 NaO 7 S), Orange G (Orange G, molecular formula C 6 H 9 NS) or aniline blue (Aniline Blue, molecular formula C 32 H 25 N 3 O 9 S 3 Na 2 ) one or more of the following.

[0041] In a specific embodiment of the present invention, the antibacterial agent is povidone-iodine (PVP-I), whose chemical formula is (C 6 H 9 NO)n·xI.

[0042] In a specific embodiment of the present invention, the phosphate buffer is a PBS buffer, comprising sodium dihydrogen phosphate (NaH 2 PO 4 ), disodium hydrogen phosphate (Na 2 HPO 4 ), sodium chloride (NaCl) and water, pH value is 7.0 ~ 7.4.

[0043] Example 1 (1) Randomly select 20 large yellow croakers to be marked, number them, anesthetize them with anesthetics, and then mechanically elastically fix the head and tail peduncle. Select the scales on the side of the large yellow croaker's trunk near the dorsal side, such as Figure 1 As shown, the length L of the fish scales measured using an optical microscope is 1.51±0.20 mm, and the width W is 2.10±0.29 mm. The average length L is calculated. a The average width W is 1.51 mm. a It is 2.10mm.

[0044] (2) According to the measured size data, the ablation mark pattern is determined to be circular, the ablation depth is 10 μm, the ablation diameter is 50 μm, and the ablation area of ​​each fish scale is calculated to be 1962.5 μm 2 .

[0045] (3) Fix the large yellow croaker to be marked one by one under the laser transmitter, and present the side of the fish body to the working area. Use a soft sponge with good water absorption to gently wipe the area on the side of the fish body to be marked, so as to effectively remove the mucus on the fish body surface without damaging the scales. After removing the mucus on the scales, the computer is programmed according to the above data, and the laser transmitter ablates the scales according to the programmed parameters, such as Figure 2 As shown, a circular ablation point is distributed on each of the three fish scales to form a triangle.

[0046] (4) After the ablation operation is completed, use a sponge soaked in the agent to gently wipe the ablated area at intervals of 8 seconds, and let it stand for 8 seconds to ensure that the agent is fully infiltrated. The agent formula is: 3% Alizarin Red S, 7% chitosan, 1% povidone iodine and 89% phosphate buffer solution (PBS buffer, pH 7.4).

[0047] After the marking was completed, 20 large yellow croakers were returned to the culture cages. As shown in Table 1, 24 hours after the marking, the survival rate of large yellow croakers was 100%, and the mark retention rate was 100%. Another inspection three months later showed that the mark retention rate was still 100%.

[0048] Table 1 Survival and mark retention of individuals after scale marking of large yellow croaker serial number 24h survival 24h mark retention 3 Month Mark Retention 1 yes yes yes 2 yes yes yes 3 yes yes yes 4 yes yes yes 5 yes yes yes 6 yes yes yes 7 yes yes yes 8 yes yes yes 9 yes yes yes 10 yes yes yes 11 yes yes yes 12 yes yes yes 13 yes yes yes 14 yes yes yes 15 yes yes yes 16 yes yes yes 17 yes yes yes 18 yes yes yes 19 yes yes yes 20 yes yes yes Example 2 The difference from Example 1 is that different types of fish are used.

[0049] (1) Twenty striped breams to be marked were randomly selected, numbered, anesthetized with anesthetic agents, and then the head and tail peduncle were mechanically elastically fixed. The scales on the side of the lateral side of the bream's trunk near the dorsal side were selected and measured using an optical microscope. The length L of the scales was 1.81±0.21 mm and the width W was 1.09±0.15 mm. The average length L was calculated. a The average width W is 1.81 mm. a It is 1.09mm.

[0050] (2) According to the measured size data, the ablation mark pattern is determined to be circular, the ablation depth is 10 μm, the ablation diameter is 50 μm, and the ablation area of ​​each fish scale is calculated to be 1962.5 μm 2 .

[0051] (3) Fix the large yellow croaker to be marked one by one under the laser transmitter, and present the side of the fish body to the working area. Use a soft sponge with good water absorption to gently wipe the area on the side of the fish body to be marked, so as to effectively remove the mucus on the fish body surface without damaging the scales. After removing the mucus on the scales, the computer is programmed according to the above data, and the laser transmitter ablates the scales according to the programmed parameters, such as Figure 3 As shown, a circular ablation point is distributed on each of the three fish scales to form a triangle.

[0052] (4) After the ablation operation is completed, use a sponge soaked in the agent to gently wipe the ablated area at intervals of 8 seconds, and let it stand for 8 seconds to ensure that the agent is fully infiltrated. The agent formula is: 3% Alizarin Red S, 7% chitosan, 1% povidone iodine and 89% phosphate buffer solution (PBS buffer, pH 7.4).

[0053] After the tagging was completed, 20 striped rock bream were returned to the culture cages. As shown in Table 2, 24 hours after tagging, the survival rate of striped rock bream was 100%, and the tag retention rate was 100%. Another inspection 3 months later showed that the tag retention rate was still 100%.

[0054] Table 2 Survival and mark retention of individual rock snapper after scale marking serial number 24h survival 24h mark retention 3 Month Mark Retention 1 yes yes yes 2 yes yes yes 3 yes yes yes 4 yes yes yes 5 yes yes yes 6 yes yes yes 7 yes yes yes 8 yes yes yes 9 yes yes yes 10 yes yes yes 11 yes yes yes 12 yes yes yes 13 yes yes yes 14 yes yes yes 15 yes yes yes 16 yes yes yes 17 yes yes yes 18 yes yes yes 19 yes yes yes 20 yes yes yes Example 3 The difference from Example 1 is that the ablation degree is different and a different formulation of medicine is used.

[0055] (1) Randomly select 20 large yellow croakers to be marked, number them, anesthetize them with anesthetics, and then mechanically elastically fix their heads and tail peduncles. Select the fish scales on the side of the large yellow croaker's trunk near the dorsal side, and the average length L of the scalesa The average width W is 1.51 mm. a It is 2.10mm.

[0056] (2) According to the measured size data, the ablation mark pattern is determined to be circular, the ablation depth is 15 μm, the ablation diameter is 70 μm, and the ablation area of ​​each fish scale is calculated to be 3846.5 μm 2 .

[0057] (3) Fix the large yellow croaker to be marked one by one under the laser transmitter, and present the side of the fish body to the working area. Use a soft sponge with good water absorption to gently wipe the area on the side of the fish body to be marked, so as to effectively remove the mucus on the fish body surface without damaging the scales. After removing the mucus on the scales, the computer is programmed according to the above data, and the laser transmitter ablates the scales according to the programmed parameters, and a circular ablation point is distributed on each of the three fish scales to form a triangle.

[0058] (4) After the ablation operation is completed, use a sponge soaked in the agent to gently wipe the ablated area at intervals of 8 seconds, and let it stand for 8 seconds to ensure that the agent is fully infiltrated. The agent formula is: 2% Alizarin Red S, 8% chitosan, 2% povidone iodine and 88% phosphate buffer solution (PBS buffer, pH 7.4).

[0059] Example 4 The difference from Example 1 is that the ablation degree is different and a different formulation of medicine is used.

[0060] (1) Randomly select 20 large yellow croakers to be marked, number them, anesthetize them with anesthetics, and then mechanically elastically fix their heads and tail peduncles. Select the fish scales on the side of the large yellow croaker's trunk near the dorsal side, and the average length L of the scales a The average width W is 1.51 mm. a It is 2.10mm.

[0061] (2) According to the measured size data, the ablation mark pattern is determined to be circular, the ablation depth is 10 μm, the ablation diameter is 100 μm, and the ablation area of ​​each fish scale is calculated to be 7850 μm 2 .

[0062] (3) Fix the large yellow croaker to be marked one by one under the laser transmitter, and present the side of the fish body to the working area. Use a soft sponge with good water absorption to gently wipe the area on the side of the fish body to be marked, so as to effectively remove the mucus on the fish body surface without damaging the scales. After removing the mucus on the scales, the computer is programmed according to the above data, and the laser transmitter ablates the scales according to the programmed parameters, and a circular ablation point is distributed on each of the three fish scales to form a triangle.

[0063] (4) After the ablation operation is completed, use a sponge soaked in the agent to gently wipe the ablated area at intervals of 8 seconds, and let it stand for 8 seconds after wiping to ensure that the agent is fully infiltrated. The agent formula is: 1% Alizarin Red S, 10% chitosan, 3% povidone iodine and 86% phosphate buffer solution (PBS buffer, pH 7.4).

[0064] Comparative Example 1 The difference from Example 1 is that the ablation degree is too low.

[0065] (1) Randomly select 20 large yellow croakers to be marked, number them, anesthetize them with anesthetics, and then mechanically elastically fix their heads and tail peduncles. Select the fish scales on the side of the large yellow croaker's trunk near the dorsal side, and the average length L of the scales a The average width W is 1.51 mm. a It is 2.10mm.

[0066] (2) According to the measured size data, the ablation mark pattern is determined to be circular, the ablation depth is 4 μm, the ablation diameter is 25 μm, and the ablation area of ​​each fish scale is calculated to be 500 μm 2 .

[0067] (3) Fix the large yellow croaker to be marked one by one under the laser transmitter, and present the side of the fish body to the working area. Use a soft sponge with good water absorption to gently wipe the area on the side of the fish body to be marked, so as to effectively remove the mucus on the fish body surface without damaging the scales. After removing the mucus on the scales, the computer is programmed according to the above data, and the laser transmitter ablates the scales according to the programmed parameters, and a circular ablation point is distributed on each of the three fish scales to form a triangle.

[0068] (4) After the ablation operation is completed, use a sponge soaked in the agent to gently wipe the ablated area at intervals of 8 seconds, and let it stand for 8 seconds to ensure that the agent is fully infiltrated. The agent formula is: 3% Alizarin Red S, 7% chitosan, 1% povidone iodine and 89% phosphate buffer solution (PBS buffer, pH 7.4).

[0069] Comparative Example 2 The difference from Example 1 is that no drug wiping was performed.

[0070] (1) Randomly select 20 large yellow croakers to be marked, number them, anesthetize them with anesthetics, and then mechanically elastically fix their heads and tail peduncles. Select the fish scales on the side of the large yellow croaker's trunk near the dorsal side, and the average length L of the scales a The average width W is 1.51 mm. a It is 2.10mm.

[0071] (2) According to the measured size data, the ablation mark pattern is determined to be circular, the ablation depth is 10 μm, the ablation diameter is 50 μm, and the ablation area of ​​each fish scale is calculated to be 1962.5 μm 2 .

[0072] (3) Fix the large yellow croaker to be marked one by one under the laser transmitter, and present the side of the fish body to the working area. Use a soft sponge with good water absorption to gently wipe the area on the side of the fish body to be marked, so as to effectively remove the mucus on the fish body surface without damaging the scales. After removing the mucus on the scales, the computer is programmed according to the above data, and the laser transmitter ablates the scales according to the programmed parameters, and a circular ablation point is distributed on each of the three fish scales to form a triangle.

[0073] Comparative Example 3 The difference from Example 1 is that the degree of drug staining is too low.

[0074] (1) Randomly select 20 large yellow croakers to be marked, number them, anesthetize them with anesthetics, and then mechanically elastically fix their heads and tail peduncles. Select the fish scales on the side of the large yellow croaker's trunk near the dorsal side, and the average length L of the scales a The average width W is 1.51 mm. a It is 2.10mm.

[0075] (2) According to the measured size data, the ablation mark pattern is determined to be circular, the ablation depth is 10 μm, the ablation diameter is 50 μm, and the ablation area of ​​each fish scale is calculated to be 1962.5 μm 2 .

[0076] (3) Fix the large yellow croaker to be marked one by one under the laser transmitter, and present the side of the fish body to the working area. Use a soft sponge with good water absorption to gently wipe the area on the side of the fish body to be marked, so as to effectively remove the mucus on the fish body surface without damaging the scales. After removing the mucus on the scales, the computer is programmed according to the above data, and the laser transmitter ablates the scales according to the programmed parameters, and a circular ablation point is distributed on each of the three fish scales to form a triangle.

[0077] (4) After the ablation operation is completed, use a sponge soaked in the agent to gently wipe the ablated area at intervals of 8 seconds, and let it stand for 8 seconds to ensure that the agent is fully infiltrated. The agent formula is: 0.5% Alizarin Red S, 7% chitosan, 1% povidone iodine and 89% phosphate buffer solution (PBS buffer, pH 7.4).

[0078] Comparative Example 4 The difference from Example 1 is that the concentration of chitosan in the medicament is lower.

[0079] (1) Randomly select 20 large yellow croakers to be marked, number them, anesthetize them with anesthetics, and then mechanically elastically fix their heads and tail peduncles. Select the fish scales on the side of the large yellow croaker's trunk near the dorsal side, and the average length L of the scales a The average width W is 1.51 mm. a It is 2.10mm.

[0080] (2) According to the measured size data, the ablation mark pattern is determined to be circular, the ablation depth is 10 μm, the ablation diameter is 50 μm, and the ablation area of ​​each fish scale is calculated to be 1962.5 μm 2 .

[0081] (3) Fix the large yellow croaker to be marked one by one under the laser transmitter, and present the side of the fish body to the working area. Use a soft sponge with good water absorption to gently wipe the area on the side of the fish body to be marked, so as to effectively remove the mucus on the fish body surface without damaging the scales. After removing the mucus on the scales, the computer is programmed according to the above data, and the laser transmitter ablates the scales according to the programmed parameters, and a circular ablation point is distributed on each of the three fish scales to form a triangle.

[0082] (4) After the ablation operation is completed, use a sponge soaked in the agent to gently wipe the ablated area at intervals of 8 seconds, and let it stand for 8 seconds to ensure that the agent is fully infiltrated. The agent formula is: 3% Alizarin Red S, 3% chitosan, 1% povidone iodine and 96% phosphate buffer solution (PBS buffer, pH 7.4).

[0083] Table 3 24h survival rate 24h Marking Retention Rate 3-month mark retention rate Example 1 100％ 100％ 100％ Example 3 100％ 100％ 100％ Example 4 100％ 100％ 100％ Comparative Example 1 100％ 85% (3 tail unretained markers) 60% (8 tail unreserved markers) Comparative Example 2 90% (2 did not survive) 90% (2 tails not retained) 75% (5 tails not retained) Comparative Example 3 100％ 90% (2 tails not retained) 85% (3 tail unretained markers) Comparative Example 4 95% (1 did not survive) 95% (1 tail unretained marker) 90% (2 tails not retained) According to Table 1, the individual survival and mark retention of 20 large yellow croakers after scale marking in Examples 3-4 and Comparative Examples 1-4 were recorded, and the results were statistically analyzed. As shown in Table 3, similar to Example 1, the 20 large yellow croakers in Examples 3-4 were able to obtain a 100% survival rate and mark retention rate after scale marking for 24 hours, indicating that within the scope of the present invention, by combining laser ablation and special agent treatment, a lasting mark can be formed on the surface of the fish body, while improving the mark retention rate, causing less damage to the fish body, with the significant advantages of high mark retention rate, low operating cost, and little impact on the fish body, providing a new technical option for fishery management and fish resource protection. In addition, this method does not require complex DNA or element analysis, nor does it require additional markings to distinguish the released population, effectively overcoming the limitations of traditional marking methods.

[0084] The mark retention rate obtained in Comparative Example 1 is lower, indicating that too low ablation degree will not be conducive to good mark durability, and even after special agent treatment, a high mark retention rate cannot be maintained, especially after 3 months, the mark retention rate is significantly reduced. Comparative Examples 2-4 show that agent treatment will also affect the mark retention rate, and the components in the agent will also reduce the damage of laser to the fish body and improve the survival rate of the fish body.

[0085] The above are only preferred embodiments of the present invention, and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the specification of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A fish scale marking method, characterized in that: The steps include: (1) The scales of the fish to be marked are ablated using laser to form a marking pattern; (2) After the ablation operation is performed, the marking pattern is wiped with a reagent; by mass fraction, the reagent includes 1-3% dye, 5-10% chitosan and 1-3% antibacterial agent, and the rest is phosphate buffer; (3) Let it sit for a while and complete the marking.

2. The fish scale marking method according to claim 1, characterized in that: The fish to be marked were anesthetized before laser ablation.

3. The fish scale marking method according to claim 1, characterized in that: The depth of ablation is 5~15μm.

4. The fish scale marking method according to claim 1, characterized in that: According to the average value of the measured length of the scale L a and the average measured width W a Calculate the area of ​​a single scale S=L a *W a , the ablation area on a single scale does not exceed 0.3% of S.

5. The fish scale marking method according to any one of claims 1 to 4, characterized in that: The marking patterns are distributed near the dorsal and / or ventral sides of the fish body trunk.

6. The fish scale marking method according to claim 1, characterized in that: The dye comprises one or more of Alizarin Red S, Orange G and Aniline Blue.

7. The fish scale marking method according to claim 1 or 6, characterized in that: The antibacterial agent is povidone iodine.

8. The fish scale marking method according to claim 1 or 6, characterized in that: The phosphate buffer comprises sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride and water, and has a pH value of 7.0-7.

4.

9. The fish scale marking method according to claim 1, characterized in that: After the ablation operation is performed, wait for 5 to 10 seconds before wiping the agent.

10. The fish scale marking method according to claim 1 or 9, characterized in that: Dip the reagent in the marking pattern and wipe it, then let it sit for 5 to 10 seconds to complete the marking.

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