Lasioderma serricorne trapping agent and preparation method and application thereof

Abstract

The invention belongs to the technical field of mosquito attractants, and particularly relates to a lasioderma serricorne trapping agent and a preparation method and application thereof. The lasioderma serricorne trapping agent is prepared from, by weight, 7%-12% of compound amino acid, 12%-20% of carboxylic acid, 5%-10% of yeast powder, 1%-3% of vitamin B, 20%-30% of mint extract, 8%-10% of honeysuckle extract, 1%-3% of propylene glycol and 20%-35% of baking soda. The components used in the trapping agent are food-grade and are nontoxic and harmless to human bodies and the environment, so that the purpose of green deinsectization and environment-friendly trapping of the lasioderma serricorne is achieved, and the trapping agent does not affect the aroma of tobacco in the storage process.

Description

Technical Field

[0001] The present invention belongs to the technical field of mosquito attractants, and particularly relates to a tobacco beetle trapping agent and a preparation method and application thereof. Background Art

[0002] The tobacco beetle is a species of the family Psoracidae, class Hexapoda. It is a worldwide stored product pest with a complex diet. It harms grains, beans, oilseeds and other food in grain warehouses; and tobacco leaves, cigarettes, cigars and other tobacco products in tobacco warehouses.

[0003] Tobacco leaf storage is the junction of industry and agriculture in the tobacco industry. It is an indispensable aging process in the cigarette making process and is generally stored for 1-3 years. For tobacco leaves in storage, tobacco beetles are the most serious pest. Tobacco beetles not only feed on tobacco leaves, causing weight loss, but also form holes, reducing the rate of tobacco leaves; the insects and insect corpses left behind will produce odors, and after burning, they will form harmful substances, reducing the availability of tobacco leaves. Tobacco beetles are also the main pests that cause the most serious damage in the production and processing of cigarettes. They can directly harm finished cigarettes, causing holes and yellow spots on cigarettes, affecting product quality. As competition in my country's cigarette industry intensifies, the monitoring and prevention methods of tobacco storage pests have directly affected the survival of cigarette brands and even the success or failure of business operations.

[0004] Therefore, it is very necessary to provide a trapping agent which not only has a significant attractant effect on tobacco beetles but also does not affect the aroma of tobacco leaves. Summary of the invention

[0005] The present invention aims to solve at least one of the technical problems existing in the above-mentioned prior art. To this end, the present invention provides a tobacco beetle trapping agent and a preparation method and application thereof, wherein the indoor trapping rate of the trapping agent for tobacco beetles is not less than 90%.

[0006] A tobacco beetle trap, calculated by weight of the trap, consists of 7-12% complex amino acids, 12-20% carboxylic acids, 5-10% yeast powder, 1-3% B vitamins, 20-30% peppermint extract, 8-10% honeysuckle extract, 1-3% propylene glycol, and 20-35% baking soda; preferably, by weight, the complex amino acids are 3 parts of leucine, 20 parts of alanine, 12 parts of lysine, and 8 parts of phenylalanine.

[0007] Preferably, the carboxylic acids consist of lactic acid, acetic acid, butyric acid and isovaleric acid.

[0008] Preferably, the weight ratio of lactic acid, acetic acid, butyric acid and isovaleric acid is 1-2:4-5:2-3:2.

[0009] Preferably, the mint extract is extracted with supercritical carbon dioxide, and the specific method is: weigh 100.0g of the prepared mint leaves, set the separation pressure of separation kettle I and separation kettle II and the pressure in the supercritical carbon dioxide cylinder to 5MPa, the temperature of separation kettle I is 40°C, and the temperature of separation kettle II is 45°C; the temperature of the extraction kettle is 40°C, and the pressure is 35MPa; CO2, flow rate is 25kg / h, extraction time is 2.5h, collect in a dark glass container, seal with parafilm, and store in a 4°C refrigerator for use.

[0010] Preferably, the honeysuckle extract is extracted with supercritical carbon dioxide, and the specific method is: weigh 100.0 g of the prepared honeysuckle, set the separation pressure of separation kettle I and separation kettle II and the pressure in the supercritical carbon dioxide cylinder to 5 MPa, the temperature of separation kettle I is 40°C, and the temperature of separation kettle II is 45°C; the temperature of the extraction kettle is 40°C, and the pressure is 35 MPa; CO2, flow rate is 25 kg / h, extraction time is 2.5h, collect in a dark glass container, seal with parafilm, and store in a 4°C refrigerator for use.

[0011] Furthermore, a method for preparing the above-mentioned tobacco beetle trapping agent adopts the following steps: The trapping agent is prepared by combining composite amino acids, carboxylic acids, yeast powder, B vitamins, peppermint extract, honeysuckle extract, propylene glycol and baking soda.

[0012] Preferably, the complex amino acid, carboxylic acid and propylene glycol are first mixed, and then the remaining components are added and mixed to prepare the trap.

[0013] An application of the above-mentioned tobacco beetle trap, wherein the tobacco beetle trap is applied in the field of tobacco leaf storage.

[0014] Beneficial effects: (1) The present invention uses a specific type of amino acid compound to form a composite amino acid, and then cooperates with the use of other carboxylic acids (such as lactic acid), mint extract and honeysuckle extract, so that the trap prepared by the present invention has a unique luring effect on tobacco beetles, thereby significantly improving the trapping effect of the trap on tobacco beetles. The trapping rate of the trap for tobacco beetles is not less than 90%. (2) The components used in the present invention are food grade and are non-toxic and harmless to the human body and the environment, thereby achieving the purpose of green pest control and environmentally friendly trapping of tobacco beetles, and the trap described in the present invention will not affect the aroma of tobacco during storage. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Diagram of the trap where the bait is stored; Figure 2 Tobacco stacking diagram. DETAILED DESCRIPTION

[0016] In order to make the technical scheme of the present invention more clearly understood by those skilled in the art, the following examples are listed for illustration. It should be noted that the following examples do not limit the scope of protection claimed in the present invention. The raw materials, reagents or devices used in the following examples can be obtained from conventional commercial channels or can be obtained by existing known methods unless otherwise specified.

[0017] The mint extract and honeysuckle extract in the following examples were prepared by the following method: 100.0 g of crushed mint leaves were weighed, the separation pressure of separation kettle I and separation kettle II and the pressure in the supercritical carbon dioxide cylinder were set to 5 MPa, the temperature of separation kettle I was 40°C, and the temperature of separation kettle II was 45°C; the temperature of the extraction kettle was 40°C, the pressure was 35 MPa; CO2, flow rate was 25 kg / h, extraction time was 2.5 h, collected in a dark glass container, sealed with parafilm, and stored in a 4°C refrigerator for use.

[0018] Example 1 A tobacco beetle trap, which, calculated by weight of the trap, consists of 7% complex amino acids, 20% carboxylic acids, 5% yeast powder, 3% B vitamins, 20% peppermint extract, 10% honeysuckle extract, 1% propylene glycol, and 34% baking soda; The complex amino acids are 3 parts of leucine, 20 parts of alanine, 12 parts of lysine and 8 parts of phenylalanine.

[0019] The carboxylic acids consist of lactic acid, acetic acid, butyric acid and isovaleric acid in a weight ratio of 1:5:2:2.

[0020] The preparation method of the above-mentioned tobacco beetle trapping agent adopts the following steps: The composite amino acid (leucine, alanine, lysine and phenylalanine are mixed to obtain the composite amino acid), carboxylic acid and propylene glycol are mixed for 30 minutes, and then the remaining components are added and stirred for 25 minutes, and then baking soda is added and stirred for 15 minutes to obtain the trap.

[0021] Example 2 A tobacco beetle trap, which is composed of 12% complex amino acids, 12% carboxylic acids, 10% yeast powder, 1% B vitamins, 30% peppermint extract, 8% honeysuckle extract, 3% propylene glycol, and 24% baking soda, calculated by weight of the trap; the complex amino acids are 3 parts of leucine, 20 parts of alanine, 12 parts of lysine, and 8 parts of phenylalanine.

[0022] The carboxylic acids consist of lactic acid, acetic acid, butyric acid and isovaleric acid in a weight ratio of 2:4:3:2.

[0023] The preparation method of the above-mentioned tobacco beetle trapping agent adopts the following steps: The composite amino acid (leucine, alanine, lysine and phenylalanine are mixed to obtain the composite amino acid), carboxylic acid and propylene glycol are mixed, and then the remaining components are added, stirred for 30 minutes, and then baking soda is added and stirred for 15 minutes to obtain the trap.

[0024] Example 3 A tobacco beetle trap, which, calculated by weight of the trap, consists of 10% complex amino acids, 16% carboxylic acids, 8% yeast powder, 2% B vitamins, 23% mint extract, 9% honeysuckle extract, 2% propylene glycol, and 30% baking soda; In terms of weight, the compounded amino acids are 3 parts of leucine, 20 parts of alanine, 12 parts of lysine and 8 parts of phenylalanine.

[0025] The carboxylic acids consist of lactic acid, acetic acid, butyric acid and isovaleric acid in a weight ratio of 1:4:3:2.

[0026] The preparation method of the above-mentioned tobacco beetle trapping agent adopts the following steps: The composite amino acid (leucine, alanine, lysine and phenylalanine are mixed to obtain the composite amino acid), carboxylic acid and propylene glycol are mixed, and then the remaining components are added and stirred for 30 minutes, and then baking soda is added and stirred for 15 minutes to obtain the trap.

[0027] Comparative Example 1 A tobacco beetle trap, which, calculated by weight of the trap, consists of 10% complex amino acids, 16% carboxylic acids, 8% yeast powder, 2% B vitamins, 32% honeysuckle extract, 2% propylene glycol, and 30% baking soda; In terms of weight, the compounded amino acids are 3 parts of leucine, 20 parts of alanine, 12 parts of lysine and 8 parts of phenylalanine.

[0028] The carboxylic acids consist of lactic acid, acetic acid, butyric acid and isovaleric acid in a weight ratio of 1:4:3:2.

[0029] The preparation method of the above-mentioned tobacco beetle trapping agent adopts the following steps: The composite amino acid (leucine, alanine, lysine and phenylalanine are mixed to obtain the composite amino acid), carboxylic acid and propylene glycol are mixed, and then the remaining components are added and stirred for 30 minutes, and then baking soda is added and stirred for 15 minutes to obtain the trap.

[0030] Comparative Example 2 A tobacco beetle trap, which, calculated by weight of the trap, consists of 10% complex amino acids, 16% carboxylic acids, 8% yeast powder, 2% B vitamins, 32% mint extract, 2% propylene glycol, and 30% baking soda; In terms of weight, the compounded amino acids are 3 parts of leucine, 20 parts of alanine, 12 parts of lysine and 8 parts of phenylalanine.

[0031] The carboxylic acids consist of lactic acid, acetic acid, butyric acid and isovaleric acid in a weight ratio of 1:4:3:2.

[0032] The preparation method of the above-mentioned tobacco beetle trapping agent adopts the following steps: The composite amino acid (leucine, alanine, lysine and phenylalanine are mixed to obtain the composite amino acid), carboxylic acid and propylene glycol are mixed, and then the remaining components are added and stirred for 30 minutes, and then baking soda is added and stirred for 15 minutes to obtain the trap.

[0033] 1-2:4-5:2-3:2 Comparative Example 3 A tobacco beetle trap, which, calculated by weight of the trap, consists of 10% complex amino acids, 16% carboxylic acids, 8% yeast powder, 2% B vitamins, 32% mint extract, 2% propylene glycol, and 30% baking soda; In terms of weight, the compounded amino acids are 3 parts of leucine, 20 parts of alanine, 12 parts of lysine and 8 parts of phenylalanine.

[0034] The carboxylic acid consists of lactic acid, acetic acid and butyric acid in a weight ratio of 3:4:5.

[0035] The preparation method of the above-mentioned tobacco beetle trapping agent adopts the following steps: The composite amino acid (leucine, alanine, lysine and phenylalanine are mixed to obtain the composite amino acid), carboxylic acid and propylene glycol are mixed, and then the remaining components are added and stirred for 30 minutes, and then baking soda is added and stirred for 15 minutes to obtain the trap.

[0036] Product effect testing Take 10 tobacco beetle breeding cages, put a piece of white self-adhesive tape in each, and evenly sprinkle the attractants (powdered) prepared in Examples 1-3 and Comparative Examples 1-3 on the surface of the white self-adhesive tape (one breeding cage is a blank control group, without attractant), then put 100 tobacco beetles into each tobacco breeding cage, place the insect breeding cages at 30°C and a relative humidity of 80%, and count the number of tobacco beetles adhered to the surface of the white self-adhesive tape in each breeding cage after 3 days (when the tobacco beetles are attracted by the attractant to inhale the attractant, the tobacco beetles stay on the surface of the white self-adhesive tape and are easily adhered to the surface of the white self-adhesive tape). The results are shown in Table 1 (trapping rate = number of adhered tobacco beetles / 50*100%).

[0037] Table 1

[0038] As can be seen from Table 1, the trapping rates of tobacco beetles prepared by the trapping agents of Examples 1-3 of the present invention are significantly higher than those of Comparative Examples 1-3 and the blank control group. As can be seen from the results of Examples 1-3 and Comparative Examples 1-3, the types and proportions of the mint extract, honeysuckle extract and carboxylic acid in the trapping agents of the present invention have a significant effect on the trapping rate of the trapping agents.

Claims

1. A tobacco beetle trapping agent, characterized in that: The trap is calculated by weight and is composed of 7-12% complex amino acids, 12-20% carboxylic acids, 5-10% yeast powder, 1-3% B vitamins, 20-30% peppermint extract, 8-10% honeysuckle extract, 1-3% propylene glycol, and 20-35% baking soda.

2. The tobacco beetle trapping agent according to claim 1, characterized in that: In terms of weight, the composite amino acid comprises 3 parts of leucine, 20 parts of alanine, 12 parts of lysine and 8 parts of phenylalanine.

3. The tobacco beetle trapping agent according to claim 1, characterized in that: The carboxylic acids consist of lactic acid, acetic acid, butyric acid, and isovaleric acid.

4. The tobacco beetle trapping agent according to claim 3, characterized in that: The weight ratio of lactic acid, acetic acid, butyric acid and isovaleric acid is 1-2:4-5:2-3:

2.

5. The tobacco beetle trapping agent according to claim 1, characterized in that: The mint extract is extracted by supercritical carbon dioxide, and the specific method is: weigh 100.0g of crushed mint leaves, set the separation pressure of separation kettle I and separation kettle II and the pressure in the supercritical carbon dioxide cylinder to 5MPa, the temperature of separation kettle I is 40°C, and the temperature of separation kettle II is 45°C; the temperature of the extraction kettle is 40°C, and the pressure is 35MPa; CO2, flow rate is 25 kg / h, extraction time is 2.5h, collect in a dark glass container, seal with parafilm, and store in a 4°C refrigerator for use.

6. The tobacco beetle trapping agent according to claim 1, characterized in that: The honeysuckle extract is extracted by supercritical carbon dioxide, and the specific method is: weigh 100.0g of crushed honeysuckle, set the separation pressure of separation kettle I and separation kettle II and the pressure in the supercritical carbon dioxide cylinder to 5MPa, the temperature of separation kettle I is 40°C, and the temperature of separation kettle II is 45°C; the temperature of the extraction kettle is 40°C, and the pressure is 35MPa; CO2, flow rate is 25 kg / h, extraction time is 2.5h, collect in a dark glass container, seal with parafilm, and store in a 4°C refrigerator for use.

7. A method for preparing the tobacco beetle trapping agent according to claim 1 or 2, characterized in that: Use the following steps: The trapping agent is prepared by combining composite amino acids, carboxylic acids, yeast powder, B vitamins, peppermint extract, honeysuckle extract, propylene glycol and baking soda.

8. A use of the tobacco beetle trapping agent according to any one of claims 1 to 6, characterized in that: The tobacco beetle trapping agent is applied in the field of tobacco leaf storage.

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