Fish feed containing corn polysaccharide

Abstract

The invention discloses a fish feed containing corn polysaccharide, and belongs to the technical field of fish culture. The fish feed comprises fish meal, krill meal, casein, soybean meal, cottonseed protein, plant starch, oil, cellulose, a multi-vitamin and multi-mineral premix, a calcium element additive, choline chloride, amino acid and corn polysaccharide fermentation liquor. Wherein the corn polysaccharide fermentation liquor is obtained by fermenting a corn polysaccharide solution, fish soluble pulp and tea saponin through clostridium tyrobutyricum. The corn polysaccharide fermentation liquor in the fish feed contains various beneficial metabolites and active ingredients, so that the nutritional value of the feed can be improved, the intestinal health of fishes can be improved, the immune function of the fishes can be enhanced, the digestibility and the utilization rate of the fishes to the feed can be improved, the growth performance of the fishes can be further improved, and the culture benefits can be improved.

Description

Technical Field

[0001] The invention relates to the technical field of fish breeding, and in particular to fish feed containing corn polysaccharide. Background Art

[0002] Corn is the third largest food crop in the world, with global annual corn production usually exceeding 1 billion tons, and my country's corn production ranks second in the world. Corn is rich in nutrients. In addition to providing a stable food source for people, it is also used in the production and processing of animal feed.

[0003] Corn starch is generally made by soaking corn in 0.3% sulfurous acid, and then crushing, screening, precipitation, drying, grinding and other processes. It has important application value in various fields such as food industry, medicine, feed industry, etc. After preparing corn starch, a large amount of by-products will remain, from which corn polysaccharides can be extracted. Corn polysaccharides have multiple biological activities and functions such as immunomodulation and anti-oxidation. However, directly adding corn polysaccharides to fish feed may produce anti-nutritional effects and affect the digestion and absorption of other nutrients such as protein. Summary of the invention

[0004] In view of this, the object of the present invention is to provide a fish feed containing corn polysaccharides, so as to overcome the problem that corn polysaccharides are difficult to use in fish feeds in the prior art.

[0005] In a first aspect, the present invention provides a fish feed containing corn polysaccharides, comprising fish meal, krill meal, casein, soybean meal, cottonseed protein, plant starch, oils, cellulose, multivitamin and multimineral premix, calcium additive, choline chloride, amino acids and corn polysaccharide fermentation broth, wherein the corn polysaccharide fermentation broth is obtained by fermenting corn polysaccharide solution, fish slurry and tea saponin by Clostridium tyrobutyricum.

[0006] Compared with the prior art, the fish meal, krill meal, casein, soybean meal and cottonseed protein in the fish feed of the present invention can provide sufficient protein, plant starch and oil can provide sufficient sugars and fats, cellulose, multi-dimensional multi-mineral premix, calcium element additive, choline chloride, amino acids and corn polysaccharide fermentation liquid can supplement other nutrients, and each component works synergistically to provide sufficient nutrients for the growth and development of fish. In particular, the corn polysaccharide fermentation liquid in the fish feed of the present invention is obtained by fermentation of Clostridium tyrobutyricum, and the corn polysaccharide fermentation liquid contains a variety of beneficial metabolites and active ingredients, which can not only improve the nutritional value of the feed, but also improve the intestinal health of fish, enhance its immune function, and improve the digestibility and utilization rate of fish to the feed, thereby further improving the growth performance of fish and improving the breeding efficiency.

[0007] Furthermore, calculated by weight of the feed, it includes 310-330 parts of fish meal, 110-130 parts of krill meal, 75-85 parts of casein, 75-85 parts of soybean meal, 110-130 parts of cottonseed protein, 100-120 parts of plant starch, 45-55 parts of oils, 70-80 parts of cellulose, 18-22 parts of multivitamin and multimineral premix, 14-16 parts of calcium additive, 3.5-4.5 parts of choline chloride and 6-7 parts of amino acids; calculated by weight of the feed, the amount of corn polysaccharide fermentation broth added is 50-150 ml / kg.

[0008] The above technical solution further limits the content ratio of each component in the feed. Within the above content ratio range, the various components in the feed can better cooperate with each other, making the nutritional ratio in the feed more reasonable and further promoting the growth of fish.

[0009] Furthermore, the cottonseed protein is dephenolized cottonseed protein; and / or the plant starch is corn starch; and / or the oil is fish oil.

[0010] The dephenolized cottonseed protein in the above technical solution removes harmful gossypol from the cottonseed protein, thereby improving safety. The cottonseed protein contains rich protein and amino acids, as well as a certain amount of fiber, fat, vitamins and minerals, which helps to improve the palatability and nutritional value of the feed.

[0011] Corn starch is easy to digest and absorb, and can provide carbohydrates, which together with other nutrients such as protein and fat constitute a balanced feed formula. In addition, corn starch has good viscosity and can be used as a binder for feed particles to help maintain the structural integrity of the feed and prevent rapid dispersion in water.

[0012] Fish oil is a high-energy-density fat source that can provide fish with a large amount of energy to support their growth and activity. Fish oil is rich in omega-3 polyunsaturated fatty acids, which are essential for the growth and health of fish.

[0013] Furthermore, the amino acids include lysine and methionine, and the mass ratio of lysine to methionine is (4.2-4.7): (1.8-2.3); and / or the calcium element additive is calcium dihydrogen phosphate.

[0014] Lysine and methionine are additionally added in the above technical solution, so that the amino acid composition in the feed is more balanced and the nutritional needs of fish are met more accurately. In addition, the above technical solution further defines the mass ratio of lysine and methionine, and within this mass ratio range, the utilization rate of feed by fish can be further improved. Monocalcium phosphate can provide calcium and phosphorus to fish at the same time, promote the normal development of fish bones, reduce the occurrence of bone diseases, and improve the growth performance and health of fish.

[0015] Furthermore, the method for preparing corn polysaccharide fermentation broth comprises the following steps: Adding fish slurry and tea saponin into the corn polysaccharide solution, stirring evenly to obtain a mixed solution; The mixed solution is inoculated with Clostridium tyrobutyricum to carry out fermentation. After the fermentation is completed, the solution is centrifuged, the supernatant is collected, and thaumatin solution is added dropwise under stirring to obtain corn polysaccharide fermentation liquid.

[0016] The above technical solution further defines a method for preparing corn polysaccharide fermentation broth, using Clostridium tyrobutyricum to ferment a mixed solution containing corn polysaccharide, fish slurry and tea saponin, which can decompose the macromolecular substances therein into small molecular monosaccharides, oligosaccharides, amino acids, short-chain fatty acids and other components. At the same time, Clostridium tyrobutyricum will also produce metabolites such as butyric acid and acetic acid during the fermentation process. The above components can not only further improve the nutritional value of the feed, but also promote the digestion and absorption of the feed by fish and improve the intestinal health of fish. In addition, the addition of thaumatin solution can improve the taste of the feed and promote the eating of fish.

[0017] Furthermore, feed additives are added to the corn polysaccharide solution. The amount of the feed additives added is 800-900 g / kg based on the mass of the corn polysaccharide solution.

[0018] The above technical solution also adds a specific amount of feed additives to the corn polysaccharide solution. The feed additives contain a variety of nutrients such as organic acids and amino acids, which can effectively increase the types and contents of beneficial active substances in the fermentation liquid, thereby further improving the growth-promoting effect of the fermentation liquid on fish.

[0019] Furthermore, the concentration of the corn polysaccharide solution is 80-120 g / kg; based on the mass of the corn polysaccharide solution, the amount of fish slurry added is 25-75 g / kg, and the amount of tea saponin added is 5-15 g / kg.

[0020] Furthermore, fish slurry and tea saponin are added to the corn polysaccharide solution and stirred evenly to obtain a mixed solution at a temperature of 25-35° C., a stirring rate of 100-200 rpm, and a stirring time of 20-30 min.

[0021] Furthermore, the inoculation amount of Clostridium tyrobutyricum is 5% to 10% of the volume of the mixed solution, the fermentation is sealed fermentation, the fermentation temperature is 30 to 37° C., and the fermentation time is 24 to 48 hours.

[0022] Furthermore, the mass concentration of the thaumatin solution is 97-99%, and the amount of the thaumatin solution added is 100-200 µg / kg based on the mass of the supernatant; when the thaumatin solution is added dropwise under stirring, the stirring speed is 50-100 rpm, and the dropping rate is 5-10 µg / min. DETAILED DESCRIPTION

[0023] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0024] In a first aspect, an embodiment of the present invention provides a fish feed containing corn polysaccharides, comprising fish meal, krill meal, casein, soybean meal, cottonseed protein, plant starch, oil, cellulose, multivitamin and multimineral premix, calcium additive, choline chloride, amino acids and corn polysaccharide fermentation broth, wherein the corn polysaccharide fermentation broth is obtained by fermenting corn polysaccharide solution, fish slurry and tea saponin by Clostridium tyrobutyricum.

[0025] The fish feed in the above technical solution includes a variety of nutrients such as fish meal, krill meal, casein, etc. In addition to providing the three basic nutrients of protein, carbohydrates, and fat, it also provides a variety of vitamins, minerals, amino acids and other nutrients. In particular, the corn polysaccharide fermentation liquid, after fermentation by Clostridium tyrobutyricum, solves the anti-nutritional effects of corn polysaccharides and generates a variety of metabolites, further improving the nutritional value of the feed, improving the intestinal health of fish, and promoting fish growth. In addition, the corn polysaccharide fermentation liquid also contains a variety of enzymes, which can promote the decomposition of nutrients in the feed and improve the digestibility and utilization rate of the feed.

[0026] Further, on the basis of the above embodiment, the embodiment of the present invention defines the feed in terms of mass fractions, including 310-330 parts of fish meal, 110-130 parts of krill meal, 75-85 parts of casein, 75-85 parts of soybean meal, 110-130 parts of cottonseed protein, 100-120 parts of plant starch, 45-55 parts of oils, 70-80 parts of cellulose, 18-22 parts of multivitamin and multi-mineral premix, 14-16 parts of calcium additives, 3.5-4.5 parts of choline chloride and 6-7 parts of amino acids; based on the mass of the feed, the amount of corn polysaccharide fermentation broth added is 50-150 ml / kg.

[0027] The above technical solution optimizes the ratio of various components such as krill meal, casein, choline chloride, etc. in the feed, which can enable fish to absorb and fully utilize various nutritional elements in the feed, improve the utilization rate of the feed, and promote the growth and development of fish.

[0028] Among them, the fish meal in the feed can be 310 parts, 315 parts, 320 parts, 325 parts or 330 parts, preferably 315 to 325 parts. The krill meal in the feed can be 110 parts, 115 parts, 120 parts, 125 parts or 130 parts, preferably 115 to 125 parts. The casein in the feed can be 75 parts, 80 parts or 85 parts. The soybean meal in the feed can be 75 parts, 80 parts or 85 parts. The cellulose in the feed can be 70 parts, 75 parts or 80 parts. The choline chloride in the feed can be 3.5 parts, 4 parts or 4.5 parts. The multi-dimensional multi-mineral premix in the feed can be 18 parts, 19 parts, 20 parts, 21 parts or 22 parts. The multi-dimensional multi-mineral premix can be selected from the California bass premix feed 3211 produced by Lianrui Group.

[0029] For example, the amount of corn polysaccharide fermentation liquid added per kilogram of feed can be 50 ml, 100 ml or 150 ml.

[0030] Furthermore, in some embodiments, the cottonseed protein may be dephenolized cottonseed protein, and for example, its mass fraction in the feed may be 110 parts, 115 parts, 120 parts, 125 parts or 130 parts, preferably 115-125 parts.

[0031] The dephenolized cottonseed protein in the above technical solution provides rich protein and amino acids while removing harmful components therein, thereby improving safety.

[0032] In some embodiments, the plant starch can be selected from corn starch. For example, the mass fraction thereof in the feed can be 100 parts, 105 parts, 110 parts, 115 parts or 120 parts, preferably 105-115 parts.

[0033] The corn starch in the above technical solution can provide necessary sugars for the feed and, as a binder, improve the stability of the feed in water.

[0034] In some embodiments, the oil may be selected from fish oil, and for example, the weight fraction thereof in the feed may be 45 parts, 50 parts, or 55 parts.

[0035] The fish oil in the above technical solution provides sufficient fat in the feed and also provides essential fatty acids, which can promote the development of the brain and retina of the fry and improve the survival rate and growth rate of the fry.

[0036] In some embodiments, the calcium additive may be calcium dihydrogen phosphate, and for example, its mass fraction in the feed may be 14 parts, 15 parts, or 16 parts.

[0037] The calcium dihydrogen phosphate in the above technical solution provides calcium and phosphorus elements at the same time, promotes the normal development of fish bones and reduces the occurrence of bone diseases.

[0038] In some embodiments, the mass fraction of amino acids in the feed may be 6, 6.5 or 7. The amino acids include lysine and methionine, and the mass ratio of lysine to methionine is (4.2-4.7): (1.8-2.3). For example, the mass ratio of lysine to methionine may be 4.2:1.8, 4.4:2.1 or 4.7:2.3.

[0039] The lysine and methionine in the above technical solution can balance the ratio of amino acids in the feed, thereby improving the utilization rate of protein in the feed by fish and promoting the growth and development of fish.

[0040] Further, based on the above embodiment, the present embodiment defines that the method for preparing corn polysaccharide fermentation broth comprises the following steps: S1. Add fish slurry and tea saponin to the corn polysaccharide solution, and stir evenly to obtain a mixed solution.

[0041] In the above steps, the concentration of the corn polysaccharide solution is 80-120 g / kg; based on the mass of the corn polysaccharide solution, the amount of fish slurry added is 25-75 g / kg, and the amount of tea saponin added is 5-15 g / kg. That is, 25-75 g of fish slurry and 5-15 g of tea saponin are added to each kilogram of corn polysaccharide solution.

[0042] Among them, commercially available corn polysaccharide can be mixed with water to obtain a corn polysaccharide solution, and the required mass of corn polysaccharide is mixed with the required mass of water to prepare a corn polysaccharide solution of the required concentration. For example, 80-120g of corn polysaccharide can be dissolved in 880-920g of water to obtain a corn polysaccharide solution with a concentration of 80-120g / kg. In addition, corn polysaccharide can also be prepared by a preparation method known in the prior art.

[0043] Further, when fish slurry and tea saponin are added to the corn polysaccharide solution and stirred to obtain a mixed solution, the temperature is 25-35°C, the stirring rate is 100-200 rpm, and the stirring time is 20-30 min. For example, the temperature can be 25°C, 30°C or 35°C; the stirring rate can be 100 rpm, 150 rpm or 200 rpm; the stirring time can be 20 min, 25 min or 30 min; the mixed solution is stirred evenly.

[0044] The mixed solution prepared in the above steps contains corn polysaccharide, fish slurry and tea saponin at the same time. The three act synergistically as fermentation raw materials, so that the fermentation liquid obtained after fermentation contains a variety of beneficial active ingredients.

[0045] Furthermore, feed additives are also added to the corn polysaccharide solution, and the amount of feed additives added is 800-900 g / kg based on the mass of the corn polysaccharide solution. Specifically, the amount of feed additives added per kilogram of corn polysaccharide solution can be 800 g, 850 g, or 900 g. Further, in terms of nutrients, the organic matter content of the feed additive is 210 g / L, the crude protein content is 142 g / L, and the free amino acid content is 105 g / L. For example, the feed additive can be specifically selected as yam acid.

[0046] S2. Inoculating Clostridium tyrobutyricum into the mixed solution for fermentation, centrifuging after the fermentation is complete, collecting the supernatant, and dropping thaumatin solution under stirring to obtain corn polysaccharide fermentation liquid.

[0047] In the above steps, the inoculation amount of Clostridium tyrobutyricum is 5% to 10% of the volume of the mixed solution, the fermentation is sealed fermentation, the fermentation temperature is 30 to 37°C, and the fermentation time is 24 to 48 hours. For example, the inoculation amount of Clostridium tyrobutyricum can be 5%, 7% or 10% of the volume of the mixed solution; the fermentation temperature can be 30°C, 32°C, 35°C or 37°C, preferably 35 to 37°C; the fermentation time can be 24h, 36h or 48h.

[0048] In the above steps, Clostridium tyrobutyricum is used to ferment the mixed solution. Clostridium tyrobutyricum will metabolize the nutrients in the mixed solution to produce butyric acid and other metabolites. At the same time, during the fermentation process, the growth and metabolic activity of Clostridium tyrobutyricum will change the physical and chemical properties of the solution, which is beneficial to the health and growth of fish after being added to fish feed.

[0049] After the fermentation is completed, centrifuge at 3000-5000 rpm for 10-20 minutes to obtain a precipitate and a supernatant, respectively. The precipitate mainly contains cell residues and other insoluble solid impurities, and the precipitate is discarded. The supernatant is rich in metabolites produced by fermentation, and the supernatant is collected for subsequent operations.

[0050] Furthermore, the mass concentration of the thaumatin solution is 97-99%, based on the mass of the supernatant, the addition amount of the thaumatin solution is 100-200 μg / kg, the stirring speed is 50-100 rpm, and the dropping rate is 5-10 μg / min.

[0051] For example, the amount of thaumatin solution added per kilogram of supernatant can be 100µg, 150µg or 200µg, the stirring speed can be 50rpm, 75rpm or 100rpm; the drop rate can be 5µg / min, 7µg / min, or 10µg / min, to ensure that the thaumatin solution can be evenly dispersed in the supernatant.

[0052] In the above step, the thaumatin solution is added dropwise under stirring to ensure that the thaumatin is evenly distributed in the fermentation liquid, so that the thaumatin is fully mixed with the fermentation product, thereby achieving the effect of adjusting the taste of the fermentation liquid.

[0053] It should be understood that the raw materials used in the following examples are all commercially available raw materials unless otherwise specified. Example 1

[0054] A fish feed containing corn polysaccharides comprises 320g of fish meal, 120g of krill meal, 80g of casein, 80g of soybean meal, 120g of dephenolized cottonseed protein, 110g of corn starch, 50g of fish oil, 74.5g of cellulose, 20g of multivitamin and multimineral premix, 15g of monocalcium phosphate, 4g of choline chloride, 4.4g of lysine, 2.1g of methionine and 100ml of corn polysaccharide fermentation liquid.

[0055] Wherein, the preparation method of corn polysaccharide fermentation broth is as follows: Add fish slurry and tea saponin to the corn polysaccharide solution, stir at 30°C and 150 rpm for 25 minutes, and stir evenly to obtain a mixed solution; the concentration of the corn polysaccharide solution is 100 g / kg, and 75 g of fish slurry and 10 g of tea saponin are added to each kilogram of corn polysaccharide solution.

[0056] The mixed solution was inoculated with Clostridium tyrobutyricum for fermentation. The inoculation amount of Clostridium tyrobutyricum was 5% of the volume of the mixed solution. After inoculation, the fermentation container was sealed and fermented at 37° C. for 36 hours.

[0057] After the fermentation is completed, centrifuge at 4000rpm for 15 minutes, discard the precipitate, collect the supernatant, and add 97% thaumatin solution dropwise under stirring. Add 200µg thaumatin solution per kilogram of supernatant at a dropping rate of 5µg / min. Control the stirring rate at 75rpm during stirring for 15 minutes to obtain corn polysaccharide fermentation liquid. Example 2

[0058] A fish feed containing corn polysaccharides comprises 330 g of fish meal, 110 g of krill meal, 85 g of casein, 75 g of soybean meal, 110 g of dephenolized cottonseed protein, 120 g of corn starch, 45 g of fish oil, 76.5 g of cellulose, 22 g of multivitamin and multimineral premix, 16 g of monocalcium phosphate, 4.5 g of choline chloride, 4.2 g of lysine, 1.8 g of methionine and 50 ml of corn polysaccharide fermentation liquid.

[0059] Wherein, the preparation method of corn polysaccharide fermentation broth is as follows: Add fish slurry and tea saponin to the corn polysaccharide solution, stir at 25°C at a stirring rate of 100 rpm for 30 minutes, and stir evenly to obtain a mixed solution; the concentration of the corn polysaccharide solution is 120 g / kg, and 25 g of fish slurry and 5 g of tea saponin are added to each kilogram of corn polysaccharide solution.

[0060] The mixed solution was inoculated with Clostridium tyrobutyricum for fermentation. The inoculation amount of Clostridium tyrobutyricum was 7% of the volume of the mixed solution. After inoculation, the fermentation container was sealed and fermented at 35° C. for 24 hours.

[0061] After the fermentation is completed, centrifuge at 4000 rpm for 15 minutes, discard the precipitate, collect the supernatant, and add 98% thaumatin solution dropwise under stirring. 150 µg thaumatin solution is added to each kilogram of supernatant at a dropping rate of 7 µg / min. The stirring rate is controlled at 50 rpm for 10 minutes to obtain corn polysaccharide fermentation liquid. Example 3

[0062] A fish feed containing corn polysaccharides comprises 310 g of fish meal, 130 g of krill meal, 75 g of casein, 85 g of soybean meal, 130 g of dephenolized cottonseed protein, 100 g of corn starch, 55 g of fish oil, 72.5 g of cellulose, 18 g of multivitamin and multimineral premix, 14 g of monocalcium phosphate, 3.5 g of choline chloride, 4.7 g of lysine, 2.3 g of methionine and 150 ml of corn polysaccharide fermentation liquid.

[0063] Wherein, the preparation method of corn polysaccharide fermentation broth is as follows: Add fish soluble slurry, tea saponin and ethyl hexadecene to the corn polysaccharide solution, stir at 35°C at a stirring rate of 200 rpm for 20 minutes, and stir evenly to obtain a mixed solution; the concentration of the corn polysaccharide solution is 80 g / kg, and 50 g of fish soluble slurry, 15 g of tea saponin and 800 g of ethyl hexadecene are added to each kilogram of corn polysaccharide solution.

[0064] The mixed solution was inoculated with Clostridium tyrobutyricum for fermentation. The inoculation amount of Clostridium tyrobutyricum was 10% of the volume of the mixed solution. After inoculation, the fermentation container was sealed and fermented at 30° C. for 48 hours.

[0065] After the fermentation is completed, centrifuge at a speed of 4000rpm for 15 minutes, discard the precipitate, collect the supernatant, and add a thaumatin solution with a mass concentration of 99% dropwise under stirring. 100μg of thaumatin solution is added to each kilogram of supernatant at a dropping rate of 10μg / min. The stirring rate is controlled at 100rpm during stirring, and stirring is performed for 15 minutes to obtain corn polysaccharide fermentation liquid. Example 4

[0066] A fish feed containing corn polysaccharides comprises 320g of fish meal, 120g of krill meal, 80g of casein, 80g of soybean meal, 120g of dephenolized cottonseed protein, 110g of corn starch, 50g of fish oil, 74.5g of cellulose, 20g of multivitamin and multimineral premix, 15g of monocalcium phosphate, 4g of choline chloride, 4.4g of lysine, 2.1g of methionine and 100ml of corn polysaccharide fermentation liquid.

[0067] Wherein, the preparation method of corn polysaccharide fermentation broth is as follows: Add fish soluble slurry, tea saponin and ethyl hexadecene to the corn polysaccharide solution, stir at 30°C at a stirring rate of 150 rpm for 25 minutes, and stir evenly to obtain a mixed solution; the concentration of the corn polysaccharide solution is 100 g / kg, and 75 g of fish soluble slurry, 10 g of tea saponin and 900 g of ethyl hexadecene are added to each kilogram of corn polysaccharide solution.

[0068] The mixed solution was inoculated with Clostridium tyrobutyricum for fermentation. The inoculation amount of Clostridium tyrobutyricum was 5% of the volume of the mixed solution. After inoculation, the fermentation container was sealed and fermented at 37° C. for 36 hours.

[0069] After the fermentation is completed, centrifuge at 4000rpm for 15 minutes, discard the precipitate, collect the supernatant, and add 97% thaumatin solution dropwise under stirring. Add 200µg thaumatin solution per kilogram of supernatant at a dropping rate of 5µg / min. Control the stirring rate at 75rpm during stirring for 15 minutes to obtain corn polysaccharide fermentation liquid. Comparative Example 1

[0070] Compared with the feed in Example 1, the only difference is that the corn polysaccharide fermentation liquid is replaced by the corn polysaccharide solution in equal amount. Comparative Example 2

[0071] Compared with the feed in Example 1, the only difference is that when preparing the corn polysaccharide fermentation liquid, the fish slurry in the mixed solution is replaced with tea saponin in an equal amount. Comparative Example 3

[0072] Compared with the feed in Example 1, the only difference is that when preparing the corn polysaccharide fermentation liquid, an equal amount of tea saponin in the mixed solution is replaced by fish slurry. Comparative Example 4

[0073] Compared with the feed in Example 1, the only difference is that when preparing the corn polysaccharide fermentation broth, Clostridium tyrobutyricum is replaced by Clostridium butyricum in equal amounts. Test Case

[0074] The feeds in Examples 1 to 3 and Comparative Examples 1 to 4 were used to feed largemouth bass at the juvenile stage, and the initial weight of the juvenile fish was (13.45±0.06) g.

[0075] The feeding conditions are shown in Table 1: Table 1

[0076] After 60 days of feeding, the specific growth rate, fatness and feed coefficient of largemouth bass were tested. The results are shown in Table 2.

[0077] Table 2

[0078] From the above results, it can be seen that compared with Examples 1-2, after adding 1,1-dihydrochloric acid to the corn polysaccharide solution in Examples 3-4, the specific growth rate and fatness of largemouth bass were improved, and the feed coefficient decreased, indicating that the addition of 1,1-dihydrochloric acid can further improve feed utilization, and increase the growth rate and fatness of largemouth bass. After the corn polysaccharide fermentation liquid in Comparative Example 1 was replaced with an unfermented corn polysaccharide solution in an equal amount, the specific growth rate and fatness of largemouth bass decreased, and the feed coefficient increased, indicating that the present invention can improve the utilization of feed, and increase the growth rate and fatness of largemouth bass after fermenting the corn polysaccharide solution. In comparative example 2, after the fish soluble slurry in the mixed solution was replaced with tea saponin in equal amounts, and in comparative example 3, after the tea saponin in the mixed solution was replaced with fish soluble slurry in equal amounts, the specific growth rate and fatness of largemouth bass were both reduced, and the feed coefficient increased, indicating that the corn polysaccharide solution, fish soluble slurry, and tea saponin have a synergistic effect, and the fermentation liquid obtained by fermenting Clostridium tyrobutyricum using the three as raw materials can improve the utilization rate of feed, and improve the growth rate and fatness of largemouth bass. In comparative example 4, after the Clostridium tyrobutyricum was replaced with Clostridium butyricum in equal amounts, the specific growth rate and fatness of largemouth bass were both reduced, and the feed coefficient increased, indicating that the fermentation liquid obtained by fermenting Clostridium tyrobutyricum in the present invention is more conducive to the growth of fish, can improve the utilization rate of feed, and improve the growth rate and fatness of largemouth bass.

[0079] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention is described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A fish feed containing corn polysaccharide, characterized in that: The feed comprises 310-330 parts by weight of fish meal, 110-130 parts by weight of krill meal, 75-85 parts by weight of casein, 75-85 parts by weight of soybean meal, 110-130 parts by weight of cottonseed protein, 100-120 parts by weight of plant starch, 45-55 parts by weight of oil, 70-80 parts by weight of cellulose, 18-22 parts by weight of multivitamin and multimineral premix, 14-16 parts by weight of calcium element additive, 3.5-4.5 parts by weight of choline chloride and 6-7 parts by weight of amino acid; the addition amount of corn polysaccharide fermentation broth is 50-150 ml / kg by weight of the feed, wherein the corn polysaccharide fermentation broth is obtained by fermenting corn polysaccharide solution, fish slurry and tea saponin by Clostridium tyrobutyricum.

2. The fish feed according to claim 1, characterized in that: The cottonseed protein is dephenolized cottonseed protein; and / or, the plant starch is corn starch; and / or, the oil is fish oil.

3. The fish feed according to claim 1, characterized in that: The amino acids include lysine and methionine, and the mass ratio of lysine to methionine is (4.2-4.7): (1.8-2.3); and / or the calcium element additive is calcium dihydrogen phosphate.

4. The fish feed according to claim 1, characterized in that: The method for preparing the corn polysaccharide fermentation broth comprises the following steps: Adding fish slurry and tea saponin into the corn polysaccharide solution, stirring evenly to obtain a mixed solution; The mixed solution is inoculated with Clostridium tyrobutyricum to carry out fermentation. After the fermentation is completed, the solution is centrifuged to collect the supernatant, and a thaumatin solution is added dropwise under stirring to obtain a corn polysaccharide fermentation solution.

5. The fish feed according to claim 4, characterized in that: A feed additive is also added to the corn polysaccharide solution. The amount of the feed additive added is 800-900 g / kg based on the mass of the corn polysaccharide solution.

6. The fish feed according to claim 4, characterized in that: The concentration of the corn polysaccharide solution is 80-120 g / kg; based on the mass of the corn polysaccharide solution, the added amount of the fish slurry is 25-75 g / kg, and the added amount of the tea saponin is 5-15 g / kg.

7. The fish feed according to claim 4, characterized in that: When the fish slurry and tea saponin are added to the corn polysaccharide solution and stirred evenly to obtain a mixed solution, the temperature is 25-35° C., the stirring rate is 100-200 rpm, and the stirring time is 20-30 min.

8. The fish feed according to claim 4, characterized in that: The inoculation amount of Clostridium tyrobutyricum is 5% to 10% of the volume of the mixed solution, the fermentation is sealed fermentation, the fermentation temperature is 30 to 37° C., and the fermentation time is 24 to 48 hours.

9. The fish feed according to claim 4, characterized in that: The mass concentration of the thaumatin solution is 97-99%, and the amount of the thaumatin solution added is 100-200 μg / kg based on the mass of the supernatant; when the thaumatin solution is added dropwise under stirring, the stirring speed is 50-100 rpm, and the dropping rate is 5-10 μg / min.

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