Traditional Chinese medicine composite preservative film with CMC-Na as carrier and application of traditional Chinese medicine composite preservative film
By using CMC-Na carrier combined with chitosan and Yunzhi polysaccharide, the shortcomings of edible fungi preservation technology were solved, and efficient and environmentally friendly edible fungi preservation effect was achieved.
Patent Information
- Application Number
- CN202411793562.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-09
- Publication Date
- 2025-03-14
AI Technical Summary
There is a lack of effective preservation and preservation techniques for edible fungi in the prior art, especially in the use of natural, safe and environmentally friendly materials.
CMC-Na is used as a carrier, combining chitosan and Yunzhi polysaccharide to prepare a Chinese medicine composite plastic wrap. The membrane slowly releases two polysaccharide components under the action of CMC-Na carrier, has anticorrosion and antibacterial effects, and can be reused multiple times.
It realizes effective anti-corrosion and freshness of edible fungi, extends the storage time to more than 7 days. At the same time, due to the natural and environmentally friendly materials, the use of chemical reagents is avoided, and the plastic wrap is breathable and uniform, cost-effective.
Abstract
Description
Technical Field
[0001] The invention relates to the research field of edible fungus preservation application, and particularly to a traditional Chinese medicine composite fresh-keeping film taking CMC-Na as a carrier and application thereof. Background Art
[0002] Yunzhi polysaccharide is extracted from the Chinese medicinal material Yunzhi, which has certain antibacterial activity and a wide antibacterial spectrum. It is safe, natural and environmentally friendly. It is non-toxic or low-toxic to humans for preservation and fresh-keeping, has no or very low drug residues, is easily degraded by nature, has high economic and social benefits, and is worthy of vigorous promotion. Chitosan is a natural cationic polysaccharide widely present in shrimp and crab shells. It can resist the infection of microorganisms such as fungi and bacteria, is easy to biodegrade, and has no toxic side effects. In recent years, research on edible coating preservation has become a hot topic, but it is relatively rare as a preservative film material. The carrier CMC-Na (sodium carboxymethyl starch) is a film-forming material that is tasteless, safe and has no side effects. There is no technical solution in the prior art for preparing preservative film using Yunzhi polysaccharide, chitosan and CMC-Na for food preservation and fresh-keeping. Summary of the invention
[0003] The purpose of the present invention is to prepare a traditional Chinese medicine composite fresh-keeping film for preserving and keeping edible fungi with CMC-Na as a carrier. The composite fresh-keeping film contains the functional components of traditional Chinese medicine for preserving and antibacterial function, and the natural antibacterial effect of chitosan is added, so that the composite fresh-keeping film plays the role of preserving and preventing bacteria while preserving. At the same time, the two polysaccharide components in the fresh-keeping film are slowly released under the action of the CMC-Na carrier, and can be repeatedly used, which is economical, efficient and environmentally friendly.
[0004] The composite fresh-keeping film obtained by the invention is uniform, beautiful and breathable, with a thickness of only 0.1-0.2 mm. The preservation time of Pleurotus pulmonarius at room temperature can be extended to more than 7 days by using the composite fresh-keeping film. The invention is simple and convenient, has no direct contact between chemical reagents or other liquid medicines and the surface of edible fungi, and can be reused.
[0005] The process for preparing the Chinese medicine preservative film comprises the following steps:
[0006] A composite cling film containing CMC-Na, wherein the preparation method of the composite cling film comprises the following steps:
[0007] (1) mixing CMC-Na with water, stirring and mixing to obtain a CMC-Na solution; the mass volume ratio of the CMC-Na powder to water is 1 g:100 mL;
[0008] (2) adding glacial acetic acid solution and chitosan powder to the CMC-Na solution and mixing them to obtain a mixed solution A;
[0009] (3) Preparation of Coriolus versicolor polysaccharide concentrate: Mix Coriolus versicolor medicinal materials and water, extract under reflux, and concentrate to obtain Coriolus versicolor polysaccharide concentrate, then add the Coriolus versicolor polysaccharide concentrate to the mixed solution A in step (2), and mix to obtain mixed solution B;
[0010] (4) adding zinc sulfate to the mixed solution B, mixing and allowing to stand to obtain a mixed solution C;
[0011] (5) The mixed solution B is poured onto a flat plate, wherein the volume area ratio of the mixed solution B to the flat plate is 55-65:122.5, and the mixture is dried at 30-40° C. for 10-12 h to obtain a composite fresh-keeping film containing CMC-Na.
[0012] Preferably, the specific method of stirring in step (1) is: stirring for 30 minutes in a 60° C. constant temperature water bath with a stirrer.
[0013] Preferably, the glacial acetic acid solution and chitosan powder are added in step (2), the concentration of the glacial acetic acid solution is 6 mol / L, and the mass fractions of the glacial acetic acid solution and chitosan powder are 1% and 0.1% of the CMC-Na solution, respectively.
[0014] Preferably, in step (3), the reflux extraction is performed twice.
[0015] Preferably, in step (3), the liquid ratio of the Coriolus versicolor medicinal material to the solvent is 1 g: 24 mL.
[0016] Preferably, in step (4), the mass fraction of zinc sulfate is 0.08-0.12% of the mixed solution; and the standing time is 10-14 hours.
[0017] Preferably, in step (3), the volume ratio of the Coriolus versicolor polysaccharide concentrate to the mixed solution A is 2-3:50-60.
[0018] Preferably, in step (5), the volume area ratio of the mixed solution B to the flat plate is 22-24:49.
[0019] The application of the CMC-Na composite preservative film disclosed in the present invention in the preservation and / or preservation of food. Preferably, the food is edible fungi.
[0020] Beneficial effects of the present invention:
[0021] The invention uses CMC-Na as a carrier and then adds chitosan and versicolor polysaccharide to prepare a traditional Chinese medicine composite fresh-keeping film for preserving and keeping edible fungi. The composite fresh-keeping film contains the functional components of traditional Chinese medicine for preserving and antibacterial function, and the natural antibacterial effect of chitosan is added, so that the composite fresh-keeping film has the functions of preserving and preventing corrosion while keeping fresh. At the same time, the two polysaccharide components in the fresh-keeping film are slowly released under the action of the CMC-Na carrier, and can be repeatedly used, which is economical, efficient and environmentally friendly. DETAILED DESCRIPTION
[0022] In order to make the purpose, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0023] The purpose of the present invention is to prepare a traditional Chinese medicine composite fresh-keeping film for preserving and keeping edible fungi with CMC-Na as a carrier. The composite fresh-keeping film contains the functional components of traditional Chinese medicine for preserving and antibacterial function, and the natural antibacterial effect of chitosan is added, so that the composite fresh-keeping film plays the role of preserving and preventing bacteria while preserving. At the same time, the two polysaccharide components in the fresh-keeping film are slowly released under the action of the CMC-Na carrier, and can be repeatedly used, which is economical, efficient and environmentally friendly.
[0024] The composite fresh-keeping film obtained by the invention is uniform, beautiful and breathable, with a thickness of only 0.1-0.2 mm. The preservation time of Pleurotus pulmonarius at room temperature can be extended to more than 7 days by using the composite fresh-keeping film. The invention is simple and convenient, has no direct contact between chemical reagents or other liquid medicines and the surface of edible fungi, and can be reused.
[0025] Example 1
[0026] The process for preparing the Chinese medicine preservative film comprises the following steps:
[0027] (1) Weigh 2 g of CMC-Na powder into a beaker, add an appropriate amount of distilled water (the mass volume ratio of CMC-Na powder to water is 1 g:100 mL), and stir in a 55°C constant temperature water bath for 30 min with a stirrer until the sodium carboxymethyl cellulose is completely dissolved.
[0028] (2) Add 0.8% glacial acetic acid solution (6 mol / L) and 0.08% chitosan powder to the above liquid, stir in a constant temperature water bath at 55°C until the chitosan is completely dissolved, and set aside.
[0029] (3) Medicinal Materials Weigh 4 g of Versicolor versicolor medicinal materials, grind them into powder and place them in a conical flask. Add water (the mass volume ratio of Versicolor versicolor powder to water is 1 g: 24 mL) and heat under reflux for 1 hour. Repeat the operation once. After extracting twice, collect the extract and concentrate it in a water bath to finally obtain 8 mL of concentrated solution. Add the concentrated solution to the above solution and stir evenly in a constant temperature water bath at 55°C.
[0030] (4) Finally, add 0.08% zinc sulfate powder, mix well, and let the mixture stand for 10 minutes to degas.
[0031] (5) Use a pipette to draw 55 mL of the above solution, pour it onto a clean glass plate (35 cm*35 cm), spread it evenly, let it air dry horizontally until it is half dry, and dry it in an oven at 30°C for about 10 h. Carefully peel off the composite film and seal it in a plastic bag.
[0032] Example 2
[0033] The process for preparing the Chinese medicine preservative film comprises the following steps:
[0034] (1) Weigh 3 g of CMC-Na powder into a beaker, add an appropriate amount of distilled water (the mass volume ratio of CMC-Na powder to water is 1 g:100 mL), and stir in a 65°C constant temperature water bath for 40 min with a stirrer until the sodium carboxymethyl cellulose is completely dissolved.
[0035] (2) Add 1.2% glacial acetic acid solution (6 mol / L) and 0.12% chitosan powder to the above liquid, stir in a 65°C constant temperature water bath until the chitosan is completely dissolved, and set aside.
[0036] (3) Medicinal Materials Weigh 6 g of Versicolor versicolor medicinal materials, grind them into powder and place them in a conical flask. Add water (the mass volume ratio of Versicolor versicolor powder to water is 1 g: 24 mL) and heat under reflux for 1 hour. Repeat the operation once. After extracting twice, collect the extract and concentrate it in a water bath to finally obtain 12 mL of concentrated solution. Add the concentrated solution to the above solution and stir evenly in a constant temperature water bath at 65°C.
[0037] (4) Finally, add 0.12% zinc sulfate powder, mix well, and let the mixture stand for 14 hours to degas.
[0038] (5) Use a pipette to absorb 65 mL of the above solution and pour it onto a clean glass plate (35 cm*35 cm). Spread it evenly and let it air dry horizontally until it is half dry. Dry it in an oven at 40°C for about 12 h. Carefully peel off the composite film and seal it in a plastic bag.
[0039] Example 3
[0040] The process for preparing the Chinese medicine preservative film comprises the following steps:
[0041] (1) Weigh 1.2 g of CMC-Na powder into a beaker, add an appropriate amount of distilled water (the mass volume ratio of CMC-Na powder to water is 1 g:100 mL), and stir in a 56°C constant temperature water bath for 35 min with a stirrer until the sodium carboxymethyl cellulose is completely dissolved.
[0042] (2) Add 1.0% glacial acetic acid solution (6 mol / L) and 0.1% chitosan powder to the above liquid, stir in a 60°C constant temperature water bath until the chitosan is completely dissolved, and set aside.
[0043] (3) Medicinal Materials Weigh 5 g of Versicolor versicolor medicinal materials, grind them into powder and place them in a conical flask. Add water (the mass volume ratio of Versicolor versicolor powder to water is 1 g: 24 mL) and heat under reflux for 1 hour. Repeat the operation once. After extracting twice, collect the extract and concentrate it in a water bath to finally obtain 10 mL of concentrated solution. Add the concentrated solution to the above solution and stir evenly in a constant temperature water bath at 62°C.
[0044] (4) Finally, add 0.1% zinc sulfate powder, mix well, and let the mixture stand for 12 hours to degas.
[0045] (5) Use a pipette to draw 60 mL of the above solution, pour it onto a clean glass plate (35 cm*35 cm), spread it evenly, let it air dry horizontally until it is half dry, and dry it in an oven at 35°C for about 11 h. Carefully peel off the composite film and seal it in a plastic bag.
[0046] Application Examples
[0047] 60 fresh, uniform-sized Pleurotus pulmonarius fruiting bodies were divided into 3 groups: untreated group (Group A), PE cling film control group (Group B), and composite cling film group (Group C prepared in Example 1), and stored at room temperature. After 5 days, the physiological indexes of the fruiting bodies of each group were measured, and 3 replicates were performed each time.
[0048] 1. Mass loss rate: measured by weighing method;
[0049] 2. Fruiting body hardness: measured using two hardness testers;
[0050] 3. Soluble solid content: measured by handheld refractometer;
[0051] 4. Respiratory intensity: measured by static method;
[0052] 5. Malondialdehyde (MDA) content: determined by thiobarbital (TBA) method;
[0053] 6. Polyphenol oxidase (PPO) activity: determined by the catechol method.
[0054] 7. Decay index: The degree of fruit body decay is divided into 5 levels. Level 0: no decay; Level 1: 1 to 3 small rotten spots; Level 2: the decay area is between 1 / 4 and 1 / 2; Level 3: the decay area is between 1 / 2 and 3 / 4; Level 4: the decay area is >3 / 4. The decay index is calculated according to the following formula: Decay index = [∑ (level × corresponding number of decayed fruit bodies)] / total number of fruit bodies in the group.
[0055] The results showed that compared with group A, the fruiting body mass loss rate of group C was significantly reduced to about -0.82% (P<0.05), and there was no significant difference between groups B and C (P>0.05), indicating that both treatments B and C can effectively maintain the water content of the fruit. After treatment, the fruiting body hardness of group C was the best, reaching 2.2kg / cm 2 , objectively delaying the fruit decay process, and the hardness after treatment A and B was 1.1kg / cm 2 and 1.4kg / cm 2 . From the perspective of soluble solid content and respiration intensity, treatment C can effectively inhibit respiration, slow down the decrease of soluble solid mass fraction, and effectively inhibit the respiration of Pleurotus pulmonarius fruiting bodies. The MDA content in the fruiting bodies of group C was relatively the lowest (1.056 μmol / L), while that of groups A and B was 2.309 μmol / L and 1.872 μmol / L, respectively; and the PPO activity of group C was also relatively the lowest, with the specific activity order being: A (107.621 U / g) > B (94.638 U / g) > C (63.269 U / g), indicating that treatment C can effectively inhibit the activity of polyphenol oxidase, thereby delaying browning. From the perspective of decay index, compared with 0.844 in group A and 0.543 in group B, the decay index of group C fruiting bodies was also the lowest (0.362), indicating that treatment C can effectively control the decay of edible fungi fruiting bodies.
Claims
1. A composite fresh-keeping film containing CMC-Na, characterized in that: The preparation method of the composite fresh-keeping film comprises the following steps: (1) mixing CMC-Na with water, stirring and mixing to obtain a CMC-Na solution; the mass volume ratio of the CMC-Na powder to water is 1 g:100 mL; (2) adding 0.8-1.2% by mass of glacial acetic acid solution and 0.08-0.12% by mass of chitosan powder to the CMC-Na solution, and mixing them to obtain a mixed solution A; (3) Preparation of Coriolus versicolor polysaccharide concentrate: mixing Coriolus versicolor powder with water, extracting and concentrating the mixture after reflux to obtain Coriolus versicolor polysaccharide concentrate, and then adding the Coriolus versicolor polysaccharide concentrate to the mixed solution A in step (2), and mixing to obtain a mixed solution B; (4) adding zinc sulfate to the mixed solution B, mixing and allowing to stand to obtain a mixed solution C; (5) The mixed solution C is poured onto a glass plate, with the volume area ratio of the mixed solution B to the plate being 55-65:122.5, and dried at 30-40° C. for 10-12 h to obtain a composite preservative film containing CMC-Na.
2. The composite fresh-keeping film containing CMC-Na according to claim 1, characterized in that: The specific method of stirring in step (1) is: stirring for 30 minutes in a 60° C. constant temperature water bath with a stirrer.
3. The composite fresh-keeping film containing CMC-Na according to claim 1, characterized in that: In step (2), the glacial acetic acid solution and chitosan powder are added, the concentration of the glacial acetic acid solution is 6 mol / L, and the mass fractions of the glacial acetic acid solution and chitosan powder are 1% and 0.1% of the CMC-Na solution, respectively.
4. The composite fresh-keeping film containing CMC-Na according to claim 1, characterized in that: In step (3), the reflux extraction is performed twice.
5. The composite fresh-keeping film containing CMC-Na according to claim 1, characterized in that: In step (3), the ratio of the versicolor powder to the solvent is 1 g: 24 mL.
6. The CMC-Na composite fresh-keeping film according to claim 1, characterized in that: In step (3), the volume ratio of the Coriolus versicolor polysaccharide concentrate to the mixed solution A is 2-3:50-60.
7. The CMC-Na composite fresh-keeping film according to claim 1, characterized in that: In step (4), the mass fraction of zinc sulfate is 0.08-0.12% of the mixed solution; and the standing time is 10-14 hours.
8. The composite fresh-keeping film containing CMC-Na according to claim 1, characterized in that: In step (5), the volume area ratio of the mixed solution B to the flat plate is 22-24:
49.
9. Use of the CMC-Na composite preservative film according to claim 1 in food preservation and / or preservation.
10. The use according to claim 9, characterized in that: The food is edible fungi.