Composition with spot-removing effect as well as preparation method and application thereof
Through gentle extraction and treatment methods, a composition of beaded wool-blue ear grass root and birch bark extract was prepared, which solved the problem of low efficiency in extracting beaded wool-blue ear grass root ecdysterone in the prior art, and achieved excellent freckle removal effect and safety.
Patent Information
- Application Number
- CN202411848584.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-16
- Publication Date
- 2025-03-18
AI Technical Summary
The prior art is difficult to effectively extract ecdysterone from beaded silk-blue ear grass roots, resulting in the effect of removing keratin and freckles.
Beaded styrofoam blue ear grass roots were mixed with ethanol, extracted and concentrated, combined with vacuum drying and ultrafine pulverization, and prepared beaded styrofoam blue ear grass roots. Meanwhile, birch bark extract is prepared using microwave extraction and drying techniques and the two are mixed in a specific proportion to form a composition with a grammatical effect.
The composition can work quickly, remove intraepithelial pigments, inhibit the division of keratinocytes and melanocytes, significantly improve the freckle removal effect, and is gentle and safe, without toxicity to cells.
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of cosmetic preparation, and in particular to a composition with the effect of removing spots, and a preparation method and application thereof. Background Art
[0002] The blue-ear grass grows on streams, valley wetlands and wet rocks below 2,700 meters above sea level, and has strong adaptability to the environment. The blue-ear grass is rich in a variety of volatile oils, and its roots contain plant ecdysone. The ecdysone has strong permeability and can be quickly absorbed by the skin, with good effects of exfoliation, freckle removal and whitening. However, there are technical barriers in the prior art for the extraction of the blue-ear grass roots, and the blue-ear grass root extract obtained by extraction cannot meet the effects of exfoliation and freckle removal.
[0003] The present invention is proposed based on this. Summary of the invention
[0004] The purpose of the present invention is to provide a composition with the effect of removing spots and a preparation method and application thereof.
[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:
[0006] The present invention provides a composition having the effect of removing spots, comprising the following components in a mass ratio:
[0007] The ratio of the extract of blue ear grass root to the extract of birch bark is 1.5-3:1-2.
[0008] The present invention also provides a method for preparing a composition having the effect of removing spots, comprising the following steps:
[0009] (1) mixing the root of the blue-eared hyacinth with ethanol, extracting for 1 to 3 times, and combining the extracts; concentrating the extracts to a paste with a relative density of 1.2 to 1.3 at 60° C. to obtain an extract; drying and crushing the extract to obtain a blue-eared hyacinth root extract;
[0010] (2) mixing birch bark with ethanol to obtain a mixture, extracting the mixture in a microwave extractor for 90 to 100 seconds to obtain an extract; filtering the extract, concentrating the filtrate obtained by filtration to a paste with a relative density of 1.1 to 1.2 at 60° C. to obtain an extract; drying the extract to a water content of 8 to 12%, and crushing to obtain a birch bark extract;
[0011] (3) The extract of the root of the ophiopogon japonicus and the extract of the bark of the birch tree are mixed in proportion to obtain a composition having the effect of removing spots.
[0012] Preferably, the concentration of ethanol in step (1) is 70-80vt%;
[0013] The mass volume ratio of the mixture of the blue-eared sedge root and ethanol is 1 g: 3-4 mL.
[0014] Preferably, the extraction in step (1) is reflux extraction;
[0015] The temperature of the reflux extraction is 65-75°C.
[0016] Preferably, the drying in step (1) is vacuum drying;
[0017] The vacuum degree of the vacuum drying is -0.08 to -0.09 MPa;
[0018] The vacuum drying temperature is 50-60°C;
[0019] The particle size of the pulverized material is 1 to 10 μm.
[0020] Preferably, the mass volume ratio of the birch bark and ethanol mixed in step (2) is 1 g: 10-20 mL.
[0021] Preferably, the initial concentration of ethanol in step (2) is 40 to 60 vt%.
[0022] Preferably, the extraction temperature in step (2) is 80-90° C.; and the particle size of the crushed product in step (2) is 1-10 μm.
[0023] The present invention also provides application of the composition in preparing whitening and spot-lightening cosmetics.
[0024] The present invention also provides the use of the composition with spot-removing effect prepared by the preparation method in the preparation of whitening and spot-removing cosmetics.
[0025] The present invention provides a composition with the effect of removing spots, and a preparation method and application thereof. The composition for removing spots of the present invention relates to a root extract of the blue ear grass and a birch bark extract. The combination of the two can make the extract components act quickly, remove the pigment content in the epidermis, inhibit the division of keratinocytes and melanocytes, and have an excellent effect of removing spots. The method for extracting the root extract of the blue ear grass and the birch bark extract of the present invention is mild, and the active ingredients in the composition can be retained to a large extent. DETAILED DESCRIPTION
[0026] The present invention provides a composition having the effect of removing spots, comprising the following components in a mass ratio:
[0027] The ratio of the root extract of blue ear herb: the bark extract of birch is 1.5-3:1-2, preferably 2:1.5.
[0028] The present invention also provides a method for preparing a composition having the effect of removing spots, comprising the following steps:
[0029] (1) mixing the root of the blue-eared hyacinth with ethanol, extracting for 1 to 3 times, and combining the extracts; concentrating the extracts to a paste with a relative density of 1.2 to 1.3 at 60° C. to obtain an extract; drying and crushing the extract to obtain a blue-eared hyacinth root extract;
[0030] (2) mixing birch bark with ethanol to obtain a mixture, extracting the mixture in a microwave extractor for 90 to 100 seconds to obtain an extract; filtering the extract, concentrating the filtrate obtained by filtration to a paste with a relative density of 1.1 to 1.2 at 60° C. to obtain an extract; drying the extract to a water content of 8 to 12%, and crushing to obtain a birch bark extract;
[0031] (3) The extract of the root of the ophiopogon japonicus and the extract of the bark of the birch tree are mixed in proportion to obtain a composition having the effect of removing spots.
[0032] In the present invention, the concentration of ethanol in step (1) is 70-80vt%, preferably 75vt%; the mass volume ratio of the mixed root of the pearl silk blue ear grass and ethanol is 1g:3-4mL, preferably 1g:3.5mL. The extraction in step (1) is reflux extraction; the temperature of the reflux extraction is 65-75°C, preferably 70°C. The drying in step (1) is vacuum drying; the vacuum degree of the vacuum drying is -0.08 to -0.09Mpa; the temperature of the vacuum drying is 50-60°C; preferably 55°C; the particle size of the crushed is 1-10μm, preferably 5μm.
[0033] In the present invention, the mass volume ratio of the birch bark and ethanol mixed in step (2) is 1g:10-20mL, preferably 1g:15mL; the initial concentration of the ethanol is 40-60vt%, preferably 50vt%; the extraction temperature is 80-90°C, preferably 85°C; the extraction time is preferably 95s; the particle size of the crushed product in step (2) is 1-10μm, preferably 8μm.
[0034] The present invention also provides application of the composition in preparing whitening and spot-lightening cosmetics.
[0035] The present invention also provides the use of the composition with spot-removing effect prepared by the preparation method in the preparation of whitening and spot-removing cosmetics.
[0036] The solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be understood as limiting the protection scope of the present invention.
[0037] Example 1
[0038] The root of the blue-eared sedge is taken and crushed to a particle size of 10 μm. 100 g of the crushed blue-eared sedge root is mixed with 300 mL of 75 vt% ethanol, and refluxed and extracted twice at 65° C., the extracts are combined, and the extracts are concentrated under reduced pressure to a paste with a relative density of 1.2 at 60° C. to obtain an extract. The extract is placed at 60° C. and a vacuum degree of -0.08 MPa and dried to a water content of 8% to obtain an intermediate. The intermediate is placed in an ultrafine grinder and crushed to a particle size of 8 μm to obtain a blue-eared sedge root extract.
[0039] The birch bark is crushed to a particle size of 10 μm, and 100 g of the crushed birch bark is mixed with 2000 mL of 45 vt% ethanol to obtain a mixture. The mixture is placed in a 90W microwave extractor, and the temperature is set to 85°C for extraction for 95 seconds to obtain an extract. The extract is filtered, and the filtrate is concentrated to a paste with a relative density of 1.2 at 60°C under a vacuum degree of -0.08 MPa to obtain an extract. The extract is dried in an oven at 50°C to a water content of 10%, and crushed to a particle size of 8 μm by an ultrafine grinder to obtain a birch bark extract.
[0040] 1.5 g of the extract of the root of Glechoma longituba and 2 g of the extract of the bark of birch were mixed to obtain a composition having the effect of removing spots.
[0041] Example 2
[0042] According to the method of Example 1, the root extract of Glechoma longituba and the bark extract of birch were prepared.
[0043] 3 g of the root extract of the ophiopogon serrata was mixed with 1 g of the bark extract of the birch tree to obtain a composition having the effect of removing spots.
[0044] Experimental Example 1
[0045] Dilute RAW264.7 cells to 6 × 10 4 / mL, inoculated into 96-well cell culture plates, 100 μL per well, 37°C, 5% CO 2Culture in a cell culture incubator for 12 hours. The culture medium of each culture well was aspirated, and the cultured cells were divided into 3 groups, Example 1 group, Example 2 group and blank control group. 200 μL of serum-free culture medium containing 200 μg of the composition with anti-plaque effect of Example 1 was added to the cells in each culture well of Example 1, 200 μL of serum-free culture medium containing 200 μg of the composition with anti-plaque effect of Example 2 was added to the cells in each culture well of Example 2, and 200 μL of serum-free culture medium was added to the blank control group, and cultured for 24 hours. After the culture was completed, 20 μL of MTT solution was added to each culture well and cultured for another 4 hours, the liquid was aspirated, 200 μL of dimethyl sulfoxide was added to each well, and the absorbance (A) value was measured at 490 nm after mixing evenly, and the cell survival rate was calculated. Cell survival rate = (average A value of the experimental group / average A value of the blank control group) × 100%. The results are shown in Table 1.
[0046] Table 1 Effects of different treatments on cell viability
[0047] Group Survival rate% Example 1 Group 108.4 Example 2 Group 106.7
[0048] As shown in Table 1, the emulsion of Example 1 and the composition with anti-spot effect of Example 2 of the present invention are safe, mild and have no cytotoxicity.
[0049] Experimental Example 2
[0050] According to the conventional method, the compositions with anti-spot effect of Examples 1 and 2 are made into emulsions for use.
[0051] 30 volunteers with freckles on their faces were selected, including 10 males and 20 females, with an average age of 32 years old. The freckles on their faces were statistically significant. The 30 volunteers were randomly divided into 3 groups, Example 1 group, Example 2 group and control group. The volunteers in Example 1 group applied a peanut-sized emulsion containing the composition with the effect of removing freckles in Example 1 after washing their faces every morning and evening. The volunteers in Example 2 group applied a peanut-sized emulsion containing the composition with the effect of removing freckles in Example 2 after washing their faces every morning and evening. The volunteers in the control group did not apply any product after washing their faces every morning and evening. During the experiment, no other products were applied except this product. After continuous use for 21 days, the moisture and pigment content of the volunteers' faces were tested using an MPA580 skin tester on the 0th, 7th, 14th and 21st days of use. The rate of change of facial moisture and pigment. The change rate of facial moisture content = (facial moisture content on the 21st day - facial moisture content on the 0th day) / facial moisture content on the 0th day × 100%; the change rate of facial pigment content = (facial pigment content on the 21st day - facial pigment content on the 0th day) / facial pigment content on the 0th day × 100%. The results are shown in Table 2.
[0052] Table 2 Effect of different treatments on the removal of freckles
[0053] Group Moisture change rate % Pigment change rate % Example 1 Group 34.8 -16.4 Example 2 Group 32.4 -16.8 Control group 1.2 0.2
[0054] Table 2 shows that the moisture content of the face of the volunteers who used the emulsion prepared by the composition with freckle removal effect increased and the pigment content decreased, indicating that the composition of the present invention has the effect of removing freckles.
[0055] Application Example 1
[0056] Ma XX, male, 28 years old, is a volunteer of Example 1 group in Experimental Example 3. On the 21st day of the experiment, the volunteer felt that the lotion containing the composition with anti-freckle effect had obvious effect on removing freckles on his face, so he used the lotion prepared by the composition with anti-freckle effect every morning and evening after the end of the experiment. After continuing to use it for 21 days, the freckles on the volunteer's face almost disappeared, and his face became delicate and smooth. He claimed that he would use the product for a long time.
[0057] Jiang XX, Tian XX, Qi XX, Liu XX and others in Experimental Example 2 continued to use the lotion containing the composition having the effect of removing spots after the experiment.
[0058] As can be seen from the above embodiments, the present invention provides a composition with the effect of removing spots, and its preparation method and application. According to the preparation method of the present invention, a root extract of the blue-eared hyacinth with good freckle removal effect can be obtained. The blue-eared hyacinth root extract and the birch bark extract are combined to prepare cosmetics, so that the extract components can act quickly, remove the pigment content in the epidermis, inhibit the division of keratinocytes and melanocytes, and have excellent freckle removal effect.
[0059] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.
Claims
1. A composition having the effect of removing spots, characterized in that: The composition comprises the following mass ratios: The ratio of the extract of blue ear grass root to the extract of birch bark is 1.5-3:1-2.
2. A method for preparing a composition having the effect of removing spots, characterized in that: The steps include: (1) mixing the root of the blue-eared hyacinth with ethanol, extracting for 1 to 3 times, and combining the extracts; concentrating the extracts to a paste with a relative density of 1.2 to 1.3 at 60° C. to obtain an extract; drying and crushing the extract to obtain a blue-eared hyacinth root extract; (2) mixing birch bark with ethanol to obtain a mixture, extracting the mixture in a microwave extractor for 90 to 100 seconds to obtain an extract; filtering the extract, concentrating the filtrate obtained by filtration to a paste with a relative density of 1.1 to 1.2 at 60° C. to obtain an extract; drying the extract to a water content of 8 to 12%, and crushing to obtain a birch bark extract; (3) The extract of the root of the ophiopogon japonicus and the extract of the bark of the birch tree are mixed in proportion to obtain a composition having the effect of removing spots.
3. The preparation method according to claim 2, characterized in that: The concentration of the ethanol in step (1) is 70-80vt%; The mass volume ratio of the mixture of the blue-eared sedge root and ethanol is 1 g: 3-4 mL.
4. The preparation method according to claim 3, characterized in that: The extraction in step (1) is reflux extraction; The temperature of the reflux extraction is 65-75°C.
5. The preparation method according to claim 4, characterized in that: The drying in step (1) is vacuum drying; The vacuum degree of the vacuum drying is -0.08 to -0.09 MPa; The vacuum drying temperature is 50-60°C; The particle size of the pulverized material is 1 to 10 μm.
6. The preparation method according to claim 5, characterized in that: The mass volume ratio of the birch bark and ethanol mixed in step (2) is 1g:10-20mL.
7. The preparation method according to claim 6, characterized in that: The initial concentration of ethanol in step (2) is 40-60vt%.
8. The preparation method according to claim 7, characterized in that: The extraction temperature in step (2) is 80-90° C.; the particle size of the crushing in step (2) is 1-10 μm.
9. Use of the composition according to claim 1 in preparing whitening and spot-lightening cosmetics.
10. Use of the composition with spot-removing effect prepared by the preparation method according to any one of claims 2 to 8 in the preparation of whitening and spot-removing cosmetics.