Antioxidant agricultural synergist as well as production process and application thereof

Abstract

The invention relates to an anti-oxidation agricultural synergist as well as a production process and application thereof, and aims to solve the technical problems that the content of reactive oxygen species (ROS) in crops is sharply increased due to the fact that current abiotic stress can disturb normal metabolism of the crops, the ROS is highly toxic to the crops, membranes and organelles of cells are greatly damaged, and the crops are stagnated in growth and even die. The invention relates to a silicon-containing mineral additive which comprises the following components in percentage by weight: 30-70% of silicon-containing mineral; 20-50% of calcium magnesium minerals; 1-20% of a cationic substitution active mineral; the antioxidant agricultural synergist is capable of improving an antioxidant enzyme system by being applied to soil, is economical in price, is prepared by compounding a plurality of natural minerals and organic matters, and enables the antioxidant enzyme system of crops to be always in an activated state by conditioning soil and continuously supplementing medium trace elements required by the crops, so that the yield of the crops is increased, and the yield of the crops is increased. And the yield loss can be reduced in case of abnormal climate conditions occurring at any time.

Description

Technical Field

[0001] The invention relates to the field of agricultural synergists, in particular to an antioxidant agricultural synergist and a production process and application thereof. Background Art

[0002] Climate change is a serious problem on a global scale that affects the entire organism, including plants. As predicted by different climate models, climate change determines the growth and distribution rate of organisms. With the pressure of global warming, the growth and distribution rate of organisms are also being affected. Currently, the average temperature increases by 0.1-0.4℃ every 10 years. The rising temperature causes uneven precipitation, which in turn causes extreme weather (drought, floods, typhoons, etc.) in recent years, or plants are exposed to extreme temperature stress (high temperature, freezing, etc.) and increased ultraviolet (UV) radiation. These aspects have caused major global attention for sustainable agricultural practices.

[0003] Ensuring optimal crop yields while protecting natural resources in the context of climate change is considered one of the biggest challenges of this century. Abiotic stress caused by climate change refers to the adverse effects of any abiotic factors on crops under specific conditions. Such as drought, low temperature, high temperature, flooding, salinity, soil pollution, nutrient imbalance, and unfavorable soil pH. Studies have shown that abiotic stress can cause more than 60% of crop yield potential loss and is the main factor limiting yield increase. Resisting its damage is a research hotspot in advanced European and American countries.

[0004] Abiotic stress can disrupt the normal metabolism of crops and cause a sharp increase in the content of reactive oxygen free radicals (ROS) in crops. ROS are highly toxic to crops and greatly damage cell membranes and organelles, causing crop growth stagnation or even death. This is physiologically called "oxidative stress". Therefore, enhancing the ability of crops to clear ROS and reduce oxidative stress in crops means enhancing the resistance of crops to abiotic stress.

[0005] In recent years, many technical solutions for improving the antioxidant system of crops have been studied and reported. Chinese patents (application numbers: CN105961104B and CN105993436B) disclose a method for growing rice with high SOD content and fertilizer for planting. It uses amino acids, chelated trace elements and silicon fertilizers, and applies comprehensive treatment from the leaves and soil to improve the content of a series of antioxidant enzymes such as SOD in rice and wheat. Chinese patents (application number: CN107628888B) disclose drought-resistant seaweed liquid fertilizers and their preparation methods and applications, which use alginic acid to improve the drought resistance of crops. In addition, Chinese patents (application number: CN104719113A) use light to improve the accumulation of antioxidant substances in lettuce. Chinese patents (application number: CN108002924B) use cold-resistant microorganisms to improve the cold resistance of crops.

[0006] In existing research, applied research has been conducted in the direction of biostimulants (alginate), trace elements, beneficial microorganisms, etc. to enhance the antioxidant enzyme system of crops. In theory, all of the above solutions have the effect of enhancing the antioxidant enzyme system of crops. However, in actual production, the application cost and implementation process of these substances are too expensive and cumbersome, and they are not suitable for actual application scenarios when climate anomalies occur.

[0007] In fact, in the way of improving the antioxidant system of crops to enhance resistance and increase production, soil conditioners or fertilizers made of natural minerals are an effective means that has been overlooked by agricultural researchers. Led by countries such as Brazil, this research has gradually emerged since 2020, and the research enthusiasm and results have gradually increased rapidly. Starting from the fact that long-term cultivation of the soil leads to excessive weathering of soil minerals and soil depletion, it specifically supplements the rock mineral powder needed by the soil, maintains the weathering rate of the mineral powder, and repairs the soil fertility, so as to achieve the purpose of improving the antioxidant system of crops, promoting nutrient absorption, assimilation and metabolism, and increasing production and quality.

[0008] Therefore, we provide an antioxidant agricultural enhancer that can enhance the antioxidant enzyme system by applying it to the soil and is economically priced. It is compounded with a variety of natural minerals and organic matter. By conditioning the soil and continuously supplementing the trace elements required by crops, the antioxidant enzyme system of crops is always in an activated state, which can cope with abnormal climate conditions that may occur at any time and reduce yield losses. Summary of the invention

[0009] The purpose of the present invention is to overcome the shortcomings of the prior art, meet the actual needs, and provide an antioxidant agricultural synergist and its production process and application to solve the current technical problem that abiotic stress can disrupt the normal metabolism of crops and cause a sharp increase in the content of reactive oxygen free radicals (ROS) in crops. ROS is highly toxic to crops and greatly damages the membranes and organelles of cells, leading to stagnation of crop growth or even death.

[0010] In order to achieve the purpose of the present invention, the technical solution adopted by the present invention is: designing an antioxidant agricultural synergist, which comprises the following components by weight:

[0011] Silica-containing minerals: 30-70%;

[0012] Calcium and magnesium minerals: 20-50%;

[0013] Cation-exchange active minerals: 1-20%;

[0014] Rare earth minerals: 0.1-10%.

[0015] Preferably, the silicon-containing mineral is preferably 40-60%, and the silicon-containing mineral is one or more of quartz, fine river sand, diatomaceous earth, metamorphic rock, amphibious rock, mica, potassium feldspar, olivine, serpentine, hornblende and pyroxene.

[0016] Preferably, the calcium-magnesium mineral is preferably 30-40%, and the calcium-magnesium mineral is one or more of gypsum, calcite, dolomite, magnesite, limestone, talc, kieserite, and carnallite.

[0017] Preferably, the cation-exchange active mineral is preferably 5-10%, and the cation-exchange active mineral is one or more of natural graphite, zeolite, sepiolite, bentonite, montmorillonite, kaolin, and attapulgite.

[0018] Preferably, the rare earth mineral is preferably 1-2%, and the rare earth mineral contains at least one of the 15 kinds of rare earths prepared from at least one of lanthanum, cerium, praseodymium, neodymium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, and yttrium, rare earth chloride, biological rare earth, oxalate rare earth, citrate rare earth, rare earth rare earth, and southeast rare earth.

[0019] Preferably, the production process of the antioxidant agricultural synergist comprises the following steps:

[0020] S1. Grind the above ingredients into powders with a mesh size of 300 or above and mix them thoroughly according to the proportions;

[0021] S2, add 100-200 parts of distilled water into the reaction kettle, add an appropriate amount of alkaline activator under constant stirring, raise the temperature to 60-70°C, and continue stirring until the activator is completely dissolved to prepare a 10-20% w / w standby solution;

[0022] S3, adding 600-800 parts by weight of mixed mineral powder to the standby solution under stirring, controlling the temperature at 50-90° C., continuing stirring, stabilizing the pH at 8-12, reacting for 2-6 hours, and then cooling to room temperature;

[0023] S4, add 200-300 parts of acidifier to the material, continue stirring, balance the pH to 7-9, react for 2-6 hours to form an acidic material;

[0024] S6. Dehydrate and dry the reacted materials to a moisture content of ≤6%;

[0025] S7. The preparation is completed to prepare a synergist containing 20-50% silicon (calculated as SiO2), 10-20% calcium and magnesium (calculated as the sum of calcium and magnesium contents), and a cation exchange amount ≥20 cmol.

[0026] Preferably, the alkaline activator is one or more of potassium hydroxide, sodium hydroxide, potassium carbonate, sodium carbonate, calcium hydroxide, and magnesium hydroxide.

[0027] Preferably, the acidic material is a complex of an inorganic acid and an organic acid, and the organic acid comprises a complex of acetic acid, malic acid, tartaric acid, citric acid, oxalic acid, succinic acid, humic acid and polyglutamic acid;

[0028] The acetic acid, malic acid, tartaric acid, citric acid, oxalic acid, succinic acid, humic acid and polyglutamic acid can also be pure salts thereof, or organic acid fermentation broth, or dried organic acid fermentation broth. If the acidity of the organic acid is not adjusted to meet the requirements, an inorganic acid is added, preferably sulfuric acid, nitric acid or phosphoric acid.

[0029] Preferably, 300-500 parts of the antioxidant agricultural synergist obtained by the production process and 500-700 parts of organic substances are physically mixed, the organic matter content of the organic substances is not less than 30%, the water content is not more than 8%, and the crushing is not less than 200 meshes, and then granulation is carried out using a disc granulator and an extruder. Furthermore, agricultural biological bacteria can also be added, and after granulation, they are coated on the surface of the particles in the presence of a binder;

[0030] The organic substance may be one or more of humic acid, fulvic acid, mature straw, mature rice husk, mature manure, oil residue, carbonized soybean, biochar, peat, and lignite, preferably humic acid, biochar, and peat;

[0031] The agricultural biological bacteria is one or more of Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Bacillus velez, and yeast;

[0032] The adhesive is one or more of maltose, straight-chain starch, sulfonate lignin and molasses, and the dosage is 1-10 parts of the bacterial agent and 5-10 parts of the adhesive.

[0033] Preferably, a use of the antioxidant agricultural synergist of the present invention in the agricultural field is provided.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] 1. The present invention uses natural composite minerals as raw materials, and forms effective fertilizer enhancers and soil conditioners according to the core mineral composition ratio required for soil remediation through low-cost and environmentally friendly processes.

[0036] The theoretical basis is that the reason for the deterioration of soil quality may be that after years of high-intensity farming, the high-quality core minerals in the soil have been weathered and lost, resulting in a lack of effective minerals in the structure that can flocculate and bond with organic matter and humic acid, making it impossible to form effective aggregates, causing the soil to become compacted, water storage and ventilation to decrease, and microorganisms to lose attachment space; and the number of frameworks that form soil cation exchange is reduced, resulting in the loss of the soil's ability to retain inorganic nutrients and a decrease in soil fertility.

[0037] In view of this problem, the present invention provides a reasonable soil conditioning formula, the ingredients of which include supplementation of trace elements and beneficial elements in the soil, plant stress resistance stimulants (rare earth minerals), improvement of the soil's nutrient retention capacity (cation exchange minerals), and improvement of soil structure (microporous structure minerals). Through reasonable activation and proportioning, the present invention can comprehensively and effectively solve the current problems of crop yield reduction, sustainable production difficulties caused by soil fertility decline and frequent natural disasters.

[0038] The synergist has stable chemical and physical properties and can be compounded with organic biostimulants (humic acid, decomposed organic matter, etc.) and beneficial bacteria to further enhance its function. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 This is a schematic diagram of cucumber yield in Example 1 of the present invention;

[0040] Figure 2 This is a schematic diagram of the effect of the antioxidant agricultural synergist in Example 2 of the present invention on the SOD activity of cucumber seedlings under low temperature stress;

[0041] Figure 3 This is a schematic diagram of the effect of the antioxidant agricultural synergist in Example 2 of the present invention on the CAT activity of cucumber seedlings under low temperature stress;

[0042] Figure 4 This is a schematic diagram of the effect of the Meifeng novel antioxidant agricultural synergist in Example 2 of the present invention on the POD activity of cucumber seedlings under low temperature stress;

[0043] Figure 5 This is a schematic diagram showing the effect of the antioxidant agricultural synergist in Example 2 of the present invention on the MDA content of cucumber seedlings under low temperature stress;

[0044] Figure 6 This is a schematic diagram of conventional fertilization in Embodiment 3 of the present invention;

[0045] Figure 7 This is a schematic diagram of using an antioxidant agricultural synergist in Example 3 of the present invention. DETAILED DESCRIPTION

[0046] The present invention is further described below in conjunction with the accompanying drawings and embodiments:

[0047] An antioxidant agricultural synergist and its production process and application, see Figures 1 to 7 , calculated by weight, including the following components:

[0048] Silica-containing minerals: 30-70%;

[0049] Calcium and magnesium minerals: 20-50%;

[0050] Cation-exchange active minerals: 1-20%;

[0051] Rare earth minerals: 0.1-10%.

[0052] Specifically, the silicon-containing mineral is preferably 40-60%, and the silicon-containing mineral is one or more of quartz, fine river sand, diatomaceous earth, metamorphic rock, amphibious rock, mica, potassium feldspar, olivine, serpentine, amphibole and pyroxene.

[0053] More specifically, the calcium-magnesium mineral is preferably 30-40%, and the calcium-magnesium mineral is one or more of gypsum, calcite, dolomite, magnesite, limestone, talc, kieserite, and carnallite.

[0054] Furthermore, the cation-exchange active mineral is preferably 5-10%, and the cation-exchange active mineral is one or more of natural graphite, zeolite, sepiolite, bentonite, montmorillonite, kaolin, and attapulgite.

[0055] Furthermore, the rare earth minerals are preferably 1-2%, and the rare earth minerals contain at least one of the 15 kinds of rare earths prepared from at least one of lanthanum, cerium, praseodymium, neodymium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, and yttrium, rare earth chloride, biological rare earth, oxalate rare earth, citrate rare earth, rare earth rare earth, and southeast rare earth.

[0056] It is worth noting that the production process of the antioxidant agricultural synergist includes the following steps:

[0057] S1. Grind the above ingredients into powders with a mesh size of 300 or above and mix them thoroughly according to the proportions;

[0058] S2, add 100-200 parts of distilled water into the reaction kettle, add an appropriate amount of alkaline activator under constant stirring, raise the temperature to 60-70°C, and continue stirring until the activator is completely dissolved to prepare a 10-20% w / w standby solution;

[0059] S3, adding 600-800 parts by weight of mixed mineral powder to the standby solution under stirring, controlling the temperature at 50-90° C., continuing stirring, stabilizing the pH at 8-12, reacting for 2-6 hours, and then cooling to room temperature;

[0060] S4, add 200-300 parts of acidifier to the material, continue stirring, balance the pH to 7-9, react for 2-6 hours to form an acidic material;

[0061] S6. Dehydrate and dry the reacted materials to a moisture content of ≤6%;

[0062] S7. The preparation is completed to prepare a synergist containing 20-50% silicon (calculated as SiO2), 10-20% calcium and magnesium (calculated as the sum of calcium and magnesium contents), and a cation exchange amount ≥20 cmol.

[0063] It is worth noting that the alkaline activator is one or more of potassium hydroxide, sodium hydroxide, potassium carbonate, sodium carbonate, calcium hydroxide, and magnesium hydroxide.

[0064] It is worth mentioning that the acidic material is a complex of inorganic acid and organic acid, and the organic acid includes a complex of acetic acid, malic acid, tartaric acid, citric acid, oxalic acid, succinic acid, humic acid and polyglutamic acid;

[0065] Acetic acid, malic acid, tartaric acid, citric acid, oxalic acid, succinic acid, humic acid and polyglutamic acid can also be pure salts thereof, or organic acid fermentation broth, or dried organic acid fermentation broth. If the acidity adjustment of the above organic acids does not meet the requirements, an inorganic acid is added, preferably sulfuric acid, nitric acid or phosphoric acid.

[0066] It is noteworthy that 300-500 parts of the antioxidant agricultural synergist prepared by the production process and 500-700 parts of the organic material are physically and fully mixed, the organic matter content of the organic material is not less than 30%, the water content is not more than 8%, and the crushing is not less than 200 meshes, and then a disc granulator and an extruder are used for granulation. Furthermore, agricultural biological bacteria can be added, and after granulation, they are coated on the surface of the particles in the presence of a binder;

[0067] The organic substance may be one or more of humic acid, fulvic acid, mature straw, mature rice husk, mature manure, oil residue, carbonized soybean, biochar, peat, and lignite, preferably humic acid, biochar, and peat;

[0068] The agricultural biological bacteria is one or more of Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Bacillus velez, and yeast;

[0069] The adhesive is one or more of maltose, straight-chain starch, sulfonate lignin and molasses, and the dosage is 1-10 parts of the bacterial agent and 5-10 parts of the adhesive.

[0070] It is worth emphasizing that an application of the antioxidant agricultural synergist of the present invention in the agricultural field is provided.

[0071] Embodiment 1

[0072] Antioxidant agricultural synergists have an effect on increasing crop yield and quality, such as Figure 1 As shown:

[0073] 1. Test materials

[0074] Antioxidant booster

[0075] 2. Experimental treatment

[0076] (I) Effects of antioxidant enhancers on leafy vegetables (fast vegetables)

[0077]

[0078] (II) Effects of antioxidant enhancers on fruits and vegetables (greenhouse cucumbers)

[0079]

[0080] 3. Experimental field setting

[0081] The experiment was carried out at the Nankou pilot base of the Institute of Vegetables and Flowers of the Chinese Academy of Agricultural Sciences from May to December 2022. The soil is brown loam, and its nutrient content is as follows:

[0082]

[0083] (1) Quick vegetable cultivation

[0084] The width of the bed is 100 cm, the height of the bed is 20 cm, the width of the furrow is 40 cm, the spacing between rows and plants is 15 cm, and it can be harvested in 40 days. From August 15 to August 21, land preparation, drip irrigation installation, soil and water quality sampling were carried out. Soil sampling: 0-20 cm, five points of mixed samples were taken as one sample, a total of 5 samples. All treatments were fertilized with 15-15-15 sulfur-based compound fertilizer 40 kg / mu and organic fertilizer 80 kg / mu.

[0085] Variety: Qingxiu, direct sowing on August 23.

[0086] Fertilization method of antioxidant agricultural synergist: furrow application, the test area for each treatment is set at 45.5 square meters, repeated 3 times.

[0087] (2) Cucumber planting

[0088] Cucumber variety: Nongke 16, 21-hole tray seedlings, seedlings were raised on August 14; planted on August 23, land preparation, basic fertilizer application, drip irrigation installation, soil and water sampling on August 21. Soil sampling: 0-20cm, five points of mixed sampling as one sample, a total of 5 samples.

[0089] Fertilization method of antioxidant agricultural synergist: Apply before planting on August 22, apply in furrows, dig a furrow in the middle of each bed, and cover with soil after applying fertilizer.

[0090] Basic fertilizer: All treatments use 40 kg / mu of 15-15-15 sulfur-based compound fertilizer and 80 kg / mu of organic fertilizer as base fertilizer; Topdressing: 30 days after planting, alternately use 20-20-20 and 16-4-38 water-soluble fertilizers, and use drip irrigation once a week at a dosage of 5 kg / mu.

[0091] The experimental field was set up with 3 replicates for each treatment, and each treatment was 18m 2 :

[0092] 4. Test indicators

[0093] (1) Cucumber

[0094] On September 14, before the fruiting period, plant samples were collected and tested for the following indicators: Growth indicators: plant height, stem diameter, fresh weight of aboveground parts, leaf area and chlorophyll content (measured on the sixth leaf of cucumber). After entering the peak fruiting period on September 23, the cucumbers were harvested in batches until November 6, and the yield of each treatment was accumulated.

[0095] (2) Fast food

[0096] The harvest was conducted on September 27, and plant samples were collected. 2 Plants were harvested at 3 points in the area, and the yield and average plant weight were calculated.

[0097] Test results analysis

[0098] 1. Fast food test

[0099]

[0100] Different letters in the table indicate significant differences among treatments (P < 0.05)

[0101] Table 3-1 Growth indicators and yields of quick vegetable experiments

[0102] Table 3-1 shows that compared with the control, the antioxidant agricultural synergist can significantly improve the growth indicators of quick vegetables and promote growth. The plant height, spread, and single leaf area of ​​the antioxidant synergist product are significantly better than the control, with the increase rates of 9.40%, 34.39%, and 24.79% respectively. Although the chlorophyll content is also higher, it does not show significant results.

[0103] In terms of fresh weight and yield per plant, the antioxidant agricultural synergist treatment also showed a significant increase compared with the control, with an increase of 12.5%. In summary, the effect of antioxidant agricultural synergist was verified in the quick vegetable experiment and has good application value.

[0104] 2. Cucumber test

[0105] 2.1 Growth indicators

[0106] Table 3-2 Growth indicators of cucumbers before fruiting

[0107]

[0108] Different letters in the table indicate significant differences among treatments (P < 0.05)

[0109] It can be seen from the above table that the application of antioxidant agricultural synergists can improve all growth indicators of cucumber and reach significant levels in plant height, leaf area and fresh weight.

[0110] 2.2 Cucumber yield

[0111] In this experiment, the cumulative yield of cucumbers was collected for 20 days after they entered the peak fruit-bearing period. Statistics showed that the treatment with antioxidant agricultural synergist showed a significant increase in yield, with the increase reaching more than 20%.

[0112] 2.3 Summary

[0113] The experiment found that antioxidant agricultural enhancers can significantly promote the growth of cucumbers, increase leaf area and plant biomass.

[0114] Embodiment 2

[0115] Test report on low temperature damage of antioxidant agricultural synergist, such as Figure 2 , Figure 3 , Figure 4 and Figure 5 As shown:

[0116] 1. Materials and methods

[0117] 1.1 Test materials

[0118] The experiment was conducted in the artificial climate chamber of the Institute of Vegetable and Flower Research, Chinese Academy of Agricultural Sciences from April to July 2023.

[0119] The experimental material cucumber (Cucumis sativus L.) variety is Zhongnong No. 16.

[0120] The antioxidant enhancer is produced by Sichuan Meifeng Plant Nutrition Technology Co., Ltd., Hogland nutrient solution.

[0121] 1.2 Plant cultivation

[0122] First, soak the cucumber seeds at 55℃ for 4-5h, sow them in a culture dish and place them in an incubator at 27℃ for germination for 24h, then select seeds with uniform germination and size and sow them in a seedling tray mixed with peat, vermiculite and perlite. When the cotyledons are fully expanded, select seedlings with uniform growth and plant them in a culture cup containing 960g of fertilizer and substrate. The culture cup size is 10cm×10cm, and there is one plant in each pot. The nutrient solution is watered once a week.

[0123] 1.3 Experimental design

[0124] Antioxidant agricultural synergist treatment was carried out at the time of planting, and the antioxidant agricultural synergist and soil were mixed evenly. The treatment simulated the actual farmland usage of 10kg / mu, 3.6g / plant, 3 replicates per treatment, 6 pots per replicate. The control was the same as the treatment except that the antioxidant agricultural synergist was not applied. The set temperature was 25-28℃ / 15-18℃ (day / night), the relative humidity was 75% / 85%, and the light intensity was 400μmol m 2 s -1 , photoperiod 12h / 12h. When the seedlings grew to three leaves and one heart, 9 pots in each treatment were treated with low temperature (day / night temperature 10℃ / 5℃, light and humidity conditions as above), and the 9 pots were placed under the original growth conditions. Samples were taken and measured 24h after treatment.

[0125] 1.4 Measurement items and methods

[0126] Malondialdehyde (MDA) content determination: using thiobarbituric acid (TBA) colorimetric method.

[0127] Determination of antioxidant enzyme activity: Superoxide dismutase (SOD) activity was determined using the nitroblue tetrazolium (NBT) reduction method.

[0128] Peroxidase (POD) activity was determined using the guaiacol method (Li Hesheng, 2004).

[0129] Catalase (CAT) activity was determined by the hydrogen peroxide decomposition method (Cakmak & Marschner, 1992).

[0130] Statistics

[0131] The experimental data were processed and plotted using Microsoft Excel software, and statistically analyzed using SPSS13.0. Duncan's new multiple range method was used for significant difference test (P<0.05).

[0132] 2. Results and Analysis

[0133] 2.1 Effects of different dosages on SOD

[0134] according to Figure 2 As shown in the figure, under normal temperature conditions, the SOD activity of the antioxidant agricultural synergist is basically the same as that of the control, with no significant difference. However, under low temperature conditions, compared with CK, the treatment can maintain the high activity of SOD under low temperature conditions, with the SOD activity increasing by 12.32%.

[0135] 2.2 Effects of different dosages on CAT

[0136] Figure 3 As shown in the figure, the CAT activity of the treatment with exogenous addition of Meifeng's new antioxidant agricultural synergist was significantly higher than that of the CK treatment under normal temperature and low temperature stress. Under normal temperature treatment, the CAT activity of the treatment increased by 43.89% compared with the control, which was significant. Under low temperature stress, the CAT activity of the treatment increased by 68.47% compared with the CK, which was significant. It can be seen that the new antioxidant agricultural synergist has a good antioxidant function.

[0137] 2.3 Effect of different dosage on POD

[0138] according to Figure 4 As shown, the POD activity of the treated and control groups was consistent under normal temperature conditions. After low temperature treatment, the POD activity of the treated group increased by 14.03%, with a significant difference.

[0139] 2.4 Effect of different dosages on MDA

[0140] MDA is a product of crop cell membrane damage and is generally used as an important indicator to evaluate the degree of damage to crops after stress. Figure 5 It can be seen that compared with cultivation under normal temperature, the MDA content in cucumber leaves after low temperature treatment showed an increasing trend, indicating that low temperature stress caused cell membrane damage. The MDA content of the control increased by 10.9% from normal temperature treatment to low temperature stress; the treatment only increased by 2.08%, indicating that the treatment effectively protected the integrity of the crop cell membrane and alleviated the peroxidation damage caused by low temperature.

[0141] 3. Summary

[0142] In summary, the antioxidant agricultural synergist can effectively alleviate the low temperature and chilling injury of cucumber seedlings at the application rate of 3.6g / plant (equivalent to 10kg / mu).

[0143] Embodiment 3

[0144] Field trial reports of antioxidant enhancers, such as Figure 6 , Figure 7 As shown:

[0145] Test date: May 20, 2023

[0146] Test location: Dongjiayingzi Village, Luocheng Street, Shouguang City, Shandong Province

[0147] Test crop: cucumber

[0148] Usage and dosage: (Antioxidant synergist, applied as base fertilizer, each treatment area is 200 square meters)

[0149] Treatment: Antioxidant enhancer 10 kg / mu

[0150] Control: Conventional fertilization

[0151] Basic conditions of the test site:

[0152] The experimental field has been planted for 20 years, and the soil has become salinized. Inorganic fertilizers can no longer be used due to severe seedling burn. Cucumbers are planted for three consecutive years each year, and only one bag of diammonium phosphate is applied at the beginning of the year. Organic fertilizers and organic water-soluble fertilizers are used at other times. As the temperature and humidity rise, viral diseases frequently occur in the field, and the infected plants cannot be treated and can only be pulled out.

[0153] Test results:

[0154] The growth and uniformity are better than those achieved with conventional fertilization, and the incidence of viral diseases is significantly reduced.

[0155] 1. Overall performance, see Figure 6 , Figure 7 .

[0156] 2. Disease susceptibility and leaf area

[0157]

[0158] Different letters in the table indicate significant differences among treatments (P < 0.05).

[0159] Experimental summary:

[0160] 1. The incidence of cucumber virus disease is reduced, the infection rate of cucumbers with applied materials is reduced by half, and the disease resistance is enhanced.

[0161] 2. There is no seedling burning phenomenon after application, the leaves are bigger and greener, and the growth is better and more uniform.

[0162] 3. Output increased significantly.

[0163] In addition, the components designed in the present invention are all universal standard parts or components known to technical personnel in this field. Their structures and principles can be known to technical personnel through technical manuals or through conventional experimental methods. They can be fully implemented by technical personnel in this field. Needless to say, the content protected by the present invention does not involve improvements to internal structures and methods.

[0164] The embodiments of the present invention disclose preferred embodiments, but are not limited thereto. A person skilled in the art can easily understand the spirit of the present invention based on the above embodiments and make different extensions and changes. However, as long as they do not deviate from the spirit of the present invention, they are all within the protection scope of the present invention.

Claims

1. An antioxidant agricultural synergist, characterized in that: Calculated by weight, it includes the following components: Silica-containing minerals: 30-70%; Calcium and magnesium minerals: 20-50%; Cation-exchange active minerals: 1-20%; Rare earth minerals: 0.1-10%.

2. The antioxidant agricultural synergist according to claim 1, characterized in that: The silicon-containing mineral is preferably 40-60%, and the silicon-containing mineral is one or more of quartz, fine river sand, diatomaceous earth, metamorphic rock, pyroxene, mica, potassium feldspar, olivine, serpentine, hornblende and pyroxene.

3. The antioxidant agricultural synergist according to claim 1, characterized in that: The calcium-magnesium mineral is preferably 30-40%, and the calcium-magnesium mineral is one or more of gypsum, calcite, dolomite, magnesite, limestone, talc, kieserite, and carnallite.

4. The antioxidant agricultural synergist according to claim 1, characterized in that: The cation-exchange active mineral is preferably 5-10%, and the cation-exchange active mineral is one or more of natural graphite, zeolite, sepiolite, bentonite, montmorillonite, kaolin, and attapulgite.

5. The antioxidant agricultural synergist according to claim 1, characterized in that: The rare earth mineral is preferably 1-2%, and the rare earth mineral contains at least one of the 15 kinds of rare earths prepared from at least one of lanthanum, cerium, praseodymium, neodymium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, and yttrium, rare earth chloride, biological rare earth, oxalate rare earth, citrate rare earth, rare earth rare earth, and southeast rare earth.

6. The production process of the antioxidant agricultural synergist according to any one of claims 1 to 5, characterized in that: The production process includes the following steps: S1. Grind the above ingredients into powders with a mesh size of 300 or above and mix them thoroughly according to the proportions; S2, add 100-200 parts of distilled water into the reaction kettle, add an appropriate amount of alkaline activator under constant stirring, raise the temperature to 60-70°C, and continue stirring until the activator is completely dissolved to prepare a 10-20% w / w standby solution; S3, adding 600-800 parts by weight of mixed mineral powder to the standby solution under stirring, controlling the temperature at 50-90° C., continuing stirring, stabilizing the pH at 8-12, reacting for 2-6 hours, and then cooling to room temperature; S4, add 200-300 parts of acidifier to the material, continue stirring, balance the pH to 7-9, react for 2-6 hours to form an acidic material; S6. Dehydrate and dry the reacted materials to a moisture content of ≤6%; S7. After the preparation, a synergist containing 20-50% silicon, 10-20% calcium and magnesium, and a cation exchange amount ≥20 cmol is prepared.

7. The production process according to claim 6, characterized in that: The alkaline activator is one or more of potassium hydroxide, sodium hydroxide, potassium carbonate, sodium carbonate, calcium hydroxide and magnesium hydroxide.

8. The production process according to claim 6, characterized in that: The acidic material is a complex of an inorganic acid and an organic acid, wherein the organic acid comprises a complex of acetic acid, malic acid, tartaric acid, citric acid, oxalic acid, succinic acid, humic acid and polyglutamic acid; The acetic acid, malic acid, tartaric acid, citric acid, oxalic acid, succinic acid, humic acid and polyglutamic acid can also be pure salts thereof, or organic acid fermentation broth, or dried organic acid fermentation broth. If the acidity of the organic acid is not adjusted to meet the requirements, an inorganic acid is added, preferably sulfuric acid, nitric acid or phosphoric acid.

9. The production process according to claim 6, characterized in that: Physically and fully mix 300-500 parts of the antioxidant agricultural synergist obtained by the production process of claim 6 and 500-700 parts of an organic substance, wherein the organic substance has an organic matter content of not less than 30%, a water content of not more than 8%, and is crushed to not less than 200 meshes, and then granulates using a disc granulator and an extruder. Furthermore, agricultural biological bacteria can also be added, and after granulation, they are coated on the surface of the particles in the presence of a binder; The organic substance may be one or more of humic acid, fulvic acid, mature straw, mature rice husk, mature manure, oil residue, carbonized soybean, biochar, peat, and lignite, preferably humic acid, biochar, and peat; The agricultural biological bacteria is one or more of Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Bacillus velez, and yeast; The adhesive is one or more of maltose, straight-chain starch, sulfonate lignin and molasses, and the dosage is 1-10 parts of the bacterial agent and 5-10 parts of the adhesive.

10. Use of the antioxidant agricultural synergist according to any one of claims 1 to 5 in the agricultural field.

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