Method for preparing porcine rotavirus VP8 nanoparticles by using kluyveromyces marxianus and application of porcine rotavirus VP8 nanoparticles

By constructing a dual replicon expression vector suitable for Max Kluvieryces , the application limitations of this strain in the edible safety-grade yeast expression system were solved, and efficient expression of swine rotavirus VP8 nanoparticles was achieved, which significantly improved the development efficiency of swine rotavirus diarrhea subunit vaccine.

CN119685370AActive Publication Date: 2025-03-25HUAZHONG AGRI UNIV +3
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Patent Information

Application Number
CN202411831929.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-12
Publication Date
2025-03-25
Estimated Expiration
2044-12-12

AI Technical Summary

Technical Problem

The prior art has not yet provided stable expression vectors and genetic operating systems for Max Kluvieriaces, limiting the application of this strain in edible safety-grade yeast expression systems.

Method used

A double replicon expression vector suitable for Max Kluvia yeast was constructed, and porcine rotavirus VP8 nanoparticles were prepared through this vector, laying the foundation for the development of the porcine rotavirus diarrhea subunit vaccine.

Benefits of technology

The efficient expression of exogenous proteins on Max Kluvieria yeast was achieved, and stable VP8 nanoparticles with high conversion were prepared, which significantly improved the development efficiency of the subunit vaccine for porcine rotavirus diarrhea.

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Abstract

The invention belongs to the technical field of biological medicines, and discloses a method for preparing porcine rotavirus VP8 nanoparticles by using kluyveromyces marxianus and application of the porcine rotavirus VP8 nanoparticles. The dual-replicon expression vector applicable to the kluyveromyces marxianus is prepared by the applicant through screening and assembling, the vector realizes the effects of high conversion rate and high expression efficiency in the kluyveromyces marxianus, and the dual-replicon expression vector pGKD32 is shown as SEQ ID NO.2. The Kluyveromyces marxianus expression system provided by the invention is utilized by the applicant to successfully express the porcine rotavirus VP8 in a nanoparticle form, and a new thought is provided for research and development of a porcine rotavirus oral immunization strategy.
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