Application of cotton GbbHLH35 gene in plant verticillium wilt resistance

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By cloning and identifying cotton GbbHLH35 genes and using genetic technology to create transgenic plants, the problem of preventing and treating cotton verticillium wort is solved, and the resistance of plants to verticillium wort is significantly improved.

CN119685386APending Publication Date: 2025-03-25ANYANG INST OF TECH +1

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Patent Information

Application Number: CN202411931508.3
Authority / Receiving Office: CN · China
Patent Type: Applications(China)
Current Assignee / Owner
Filing Date: 2024-12-26
Publication Date: 2025-03-25

Application Information

Patent Timeline

26 Dec 2024

Application

CN202411931508.3

25 Mar 2025

Publication

CN119685386A

IPC: C12N15/82; C12N15/29; C07K14/415; A01H5/00; A01H6/20; A01H6/60

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Application Domain

Plant peptides Fermentation

Technology Topics

Biotechnology Nucleotide

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AI Technical Summary

Technical Problem

Cotton Verticillium wort is caused by Dali's Berry Bacteria, and the existing prevention and control measures are difficult to be thorough and long-term effective, resulting in damage to cotton production.

Method used

By cloning and identifying cotton GbbHLH35 genes, transgenic plants are created using gene silencing and overexpression techniques to improve plants' resistance to verinary wort.

Benefits of technology

Through the overexpression of the GbbHLH35 gene, Arabidopsis's resistance to verticillium wilt is significantly enhanced, the colonization of the diseased fingers and pathogens is reduced, and cell damage is delayed.

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Abstract

The invention discloses application of a cotton GbbHLH35 gene in improving the disease resistance of plant verticillium wilt, the nucleotide sequence of the gene is shown as SEQ ID NO: 1, and the amino acid sequence is shown as SEQ ID NO: 2. The gene is remarkably and highly expressed in cotton roots and is remarkably up-regulated under the induction of verticillium wilt, and the sequence of the gene contains a conserved motif related to disease resistance. The sensitivity of the plant to verticillium wilt is enhanced by silencing the expression of the GbbHLH35 gene in the sea island cotton. The overexpressed GbbHLH35 gene arabidopsis thaliana homozygous line is obtained through a transgenic technology, and after the overexpressed GbbHLH35 gene arabidopsis thaliana homozygous line is infected with verticillium The invention has important significance in improvement of cotton verticillium wilt resistance breeding and research, and can be applied to breeding of resistant cotton varieties.

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