Bacterial lipid droplet film rhodamine fluorescent dye as well as synthesis and application thereof

By developing the Rhodanmin bacterial lipid drop membrane fluorescent dye, the problem of difficulty in monitoring and studying the dynamic process of lipid drop membrane in the prior art is solved, and efficient, specific labeling and super-resolution fluorescence imaging of bacterial lipid drop membranes are achieved.

CN119707906AActive Publication Date: 2025-03-28DALIAN INSTITUTE OF CHEMICAL PHYSICS CHINESE ACADEMY OF SCIENCES
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Patent Information

Application Number
CN202311265281.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2023-09-27
Publication Date
2025-03-28
Estimated Expiration
2043-09-27

AI Technical Summary

Technical Problem

The prior art is difficult to effectively monitor and study the dynamic process of monolayer phospholipid membranes of lipid droplets, and lacks suitable fluorescent dyes for super-resolution imaging.

Method used

A class of Rhodamine bacterial lipid droplet membrane fluorescent dyes were developed, which increased lipid solubility and TICT effects by introducing long carbon chains and benzene rings on the nitrogen atoms of the Rhodamine fluorescent group, and combined with positive charges to achieve targeting and super-resolution imaging of bacterial lipid droplet membranes.

Benefits of technology

This fluorescent dye can efficiently and specifically label bacteria's lipid droplet film, with the advantages of low staining concentration, rapid dyeing, good biocompatibility, high fluorescence brightness and strong light stability, realizing super-resolution fluorescence imaging of bacterial lipid droplet film.

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Abstract

The invention provides a rhodamine bacteria lipid droplet membrane fluorescent dye and synthesis and application thereof, the fluorescent dye is based on the affinity of rhodamine on long carbon chains connected to nitrogen atoms to lipid, the affinity of open-loop rhodamine with positive charges to lipid membranes with negative potentials, and reduction of background of non-lipid droplet membrane parts by benzene ring enhanced TICT. The structural formula of the designed and synthesized dye (C4, C6 and C8) capable of being positioned on a bacterial lipid droplet film is shown in the formula (1), and n is one or more than two of 2, 4 or 6. According to the present invention, with the application of the dye, the lipid droplet film imaging of the bacillus cereus, the Rhodococcus and the Rhodococcus jostii RHA1 can be achieved; and with the application of the dye, the lipid droplet film imaging of the bacillus cereus, the Rhodococcus jostii and the RHA1 can be achieved.
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