Composite antibiological inoculant, and preparation method and application thereof

A composite biocontrol agent combining Pseudomonas sp. A1 and Bacillus subtilis K1 effectively addresses the instability of single microbial treatments for tomato wilt disease, achieving a 36% reduction in disease incidence by using a mixture of these bacteria in a biocontrol suspension for root inoculation.

CN119709542AInactive Publication Date: 2025-03-28HUZHOU COLLEGE
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Patent Information

Application Number
CN202510074102.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-17
Publication Date
2025-03-28
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

Current methods for controlling tomato wilt disease caused by Xanthomonas campestris are ineffective due to the bacterium's strong transmission and invasion mechanisms, and chemical pesticides provide only temporary relief, while single microbial biocontrol measures lack stability in disease control.

Method used

A composite biocontrol agent comprising at least 1×10^8 CFU/mL of Pseudomonas sp. A1 and Bacillus subtilis K1 is used, where Pseudomonas sp. A1 is preserved as CGMCC No.14027 and Bacillus subtilis K1 as CGMCC No.15341, mixed in a 1:1 ratio to create a suspension for root inoculation, enhancing biocontrol efficacy.

Benefits of technology

The composite biocontrol agent effectively delays the onset of tomato wilt disease by 36% compared to single microbial treatments, providing a stable and environmentally friendly disease control strategy.

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Abstract

The invention provides a composite biocontrol microbial inoculant and a preparation method and application thereof. The composite biocontrol microbial agent disclosed by the invention is composed of pseudomonas putida (Pseudomonas sp.) A1 and bacillus subtilis (Bacillus subtilis) K1; the pseudomonas putida A1 is preserved in the China General Microbiological Culture Collection Center on April 14, 2017, and the preservation number of the pseudomonas putida A1 is CGMCC No.14027; the bacillus subtilis K1 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on February 2, 2018, and the preservation number is CGMCC No.15341. The bacillus subtilis K1 is preserved in the China General Microbiological Culture Collection Center (CGMCC). The compound biocontrol microbial inoculum can effectively delay the occurrence of tomato bacterial wilt by adopting a bacterial liquid root irrigation method, and compared with application of a single biocontrol strain, the disease index is reduced by 36%.
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