Serum-free polypeptide culture medium for promoting proliferation of mesenchymal stem cells and preparation method of serum-free polypeptide culture medium

By mixing traditional Chinese medicine extracts and polypeptides in a specific proportion, a serum-free polypeptide culture medium was prepared, which solved the problem of the risk of viral contamination in the serum type medium in the prior art, and achieved a safe and effective adipose mesenchymal stem cell proliferation effect.

CN119709611AInactive Publication Date: 2025-03-28山东丽山生物科技有限公司
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Patent Information

Application Number
CN202510228533.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-28
Publication Date
2025-03-28
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The prior art uses serotype medium to culture fatty mesenchymal stem cells at risk of viral contamination, making it difficult to provide safe and effective serum-free polypeptide culture medium.

Method used

A serum-free polypeptide culture medium was prepared by mixing Dendrobium officinale, Cuscuta, Panax notoginseng, Astragalus, Polygonum multiflorum, ginseng, artificial musk, oyster polypeptide and Sichuan Achyranthes polypeptide polypeptide polypeptide by a specific proportion, and combined with Chinese herbal extracts and peptides to promote the proliferation of fat mesenchymal stem cells.

Benefits of technology

The serum-free polypeptide culture medium significantly promotes the proliferation of adipose mesenchymal stem cells, has good specific effects, and through the combination of specific processes and proportions, the safety and effectiveness of the culture medium are ensured.

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Abstract

The invention relates to the technical field of cell culture, and particularly provides a serum-free polypeptide culture medium for promoting proliferation of mesenchymal stem cells and a preparation method thereof, and the serum-free polypeptide culture medium for promoting proliferation of mesenchymal stem cells is prepared from dendrobium officinale, semen cuscutae, pseudo-ginseng, astragalus membranaceus, polygonum multiflorum, ginseng, artificial musk, oyster polypeptide, radix cyathulae polypeptide and the like. The serum-free polypeptide culture medium for promoting proliferation of the mesenchymal stem cells and the preparation method of the serum-free polypeptide culture medium are researched by further aiming at the culture environment of the adipose-derived mesenchymal stem cells and combining polypeptide and traditional Chinese medicine extracts.
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