Method for detecting inhibition effect of reagent on xanthine oxidase and application of method
By setting up specific inhibition experimental processes and calculation evaluation methods in primary hepatocytes, the problem of difficult extrapolation of xanthine oxidase inhibitor screening results in the prior art is solved, and more accurate assessment of inhibitory effects and effective extrapolation of human results is achieved.
CN119709939APending Publication Date: 2025-03-28REED LIVER DISEASE RES (SHANGHAI) CO LTD
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Patent Information
- Application Number
- CN202411927234.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-25
- Publication Date
- 2025-03-28
AI Technical Summary
Technical Problem
The prior art is difficult to effectively extrapolate the results in human body when screening xanthine oxidase inhibitors, resulting in differences in the screening results from the actual situation in the body.
Method used
Develop a detection reagent method based on primary hepatocytes, and efficiently and accurately evaluate the inhibitory effect of the reagent to be detected on xanthine oxidase by setting up specific inhibition experimental procedures and calculation evaluation methods.
Benefits of technology
This method can more accurately evaluate the inhibitory effect of the reagent to be tested, and because primary hepatocytes have a high physiological correlation and complete metabolic function, the results are more suitable for extrapolation in humans.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention relates to a method for detecting the inhibiting effect of a reagent on xanthine oxidase and application of the method. A brand-new method for detecting the inhibition effect of the reagent on the xanthine oxidase is designed, and a specific inhibition experiment process and a calculation evaluation method are set based on the reaction that the xanthine oxidase catalyzes xanthine to synthesize uric acid in primary hepatocytes. The inhibition effect of the to-be-detected reagent on the xanthine oxidase in the primary hepatocytes can be efficiently and accurately evaluated, and the primary hepatocytes have high physiological correlation and complete metabolic functions, so that the results in human bodies can be effectively extrapolated.
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