DNA library building method, joint element and kit

By using primers or linkers with 5' phosphorylation modification to library construction, the problem that the types and quality of tag linkers in the prior art do not meet the needs of high-throughput sequencing and library construction is solved, and high-quality library construction is achieved suitable for Illumina and MGI dual sequencing platforms, improving the uniformity of library output and data splitting.

CN119709950APending Publication Date: 2025-03-28NANODIGMBIO (NANJING) BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202510124654.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-26
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

The types and quality of tag connectors in the prior art do not meet the needs of high-throughput sequencing library building, especially when compatible with Illumina and MGI dual sequencing platforms.

Method used

The target sample was library constructed using primers or linkers with 5’ phosphorylation modification to obtain linear or circular libraries suitable for different sequencing platforms. Specific methods include further optimizing library construction by targeted capture using P5-truncated or full-length amplification primers, and P7-truncated or full-length linkers.

Benefits of technology

It realizes high-quality library construction suitable for Illumina and MGI dual sequencing platforms, improves the uniformity of library output and data splitting, and meets the higher requirements of high-throughput sequencers for the type and quality of sequencing connectors.

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Abstract

The invention provides a DNA library building method, a joint element and a kit. The method comprises the steps that a primer with 5'phosphorylation modification or a connector with 5 'phosphorylation modification is adopted to conduct library construction on a target sample, a linear amplification library with 5' phosphorylation modification is obtained, and the linear amplification library with 5 'phosphorylation modification is the linear library suitable for the Illumin sequencing platform; or further cyclizing the linear amplification library with the 5 '-phosphorylation modification to obtain a cyclized library suitable for an MGI sequencing platform. By adopting the method disclosed by the invention, balanced output control of multiple samples on a machine at the same time is facilitated, and large-scale production is more facilitated.
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