Primer group, detection method of national standard swine-derived components and application

By designing a primer set that is specifically complementary to the conserved regions of the ATP8 gene of the national standard pig and using a DBQ double quenching probe, the problems of background signal interference, insufficient sensitivity and low accuracy in the detection of animal-derived components of livestock and poultry were solved, and efficient and accurate detection results were achieved.

CN119710013APending Publication Date: 2025-03-28SANGON BIOTECH (SHANGHAI) CO LTD
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Patent Information

Application Number
CN202411601204.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-11
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

The existing quantitative PCR technology has problems of background signal interference, insufficient sensitivity and low accuracy in the detection of animal-derived components of livestock and poultry, which limits its application range and reliability.

Method used

A primer set was designed, including primer pairs and DBQ double quenching probes that were specifically complementary to the conserved regions of the national standard pig ATP8 gene, to improve the specificity and sensitivity of the detection.

Benefits of technology

By using specific primer pairs and DBQ double quenching probes, the specificity and sensitivity of quantitative PCR detection are significantly improved, the generation of false positive and false negative results are reduced, and the accuracy and reliability of the detection results are improved.

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Abstract

The invention provides a primer group, a detection method of national standard swine-derived components and application, and relates to the technical field of biological detection. The invention provides a primer group. The primer group comprises a primer pair and a DBQ dual-extraction probe, wherein the primer pair and a conserved region of a national standard pig ATP8 gene have specificity and complementarity. The primer group provided by the invention aims at a conserved region of the national standard pig ATP8 gene, high specificity and accuracy of qPCR detection are ensured, and the non-specific amplification risk is effectively reduced. The high specificity of the primer pair enables the primer pair to be capable of detecting national standard swine-derived components in a trace sample, and the DBQ double-quenching probe realizes real-time monitoring of amplification of a target DNA sequence, so that the accuracy of quantitative analysis is improved. The combination of the primer pair and the probe reduces false positive and false negative results, is crucial to food safety detection and supervision, and ensures the reliability of detection results.
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