Specific primer and system for detecting Paleocystis globosa, application, kit and detection method
By designing a molecular marker pgcp1 and specific primer probe based on the chloroplast genome, combined with RPA and LFD detection methods, the problem of spherical cystosus in the field was solved, and rapid and sensitive quantitative detection was achieved, supporting early monitoring and early warning of red tides.
CN119710076APending Publication Date: 2025-03-28INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Patent Information
- Application Number
- CN202510228205.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-28
- Publication Date
- 2025-03-28
AI Technical Summary
Technical Problem
The prior art is difficult to detect spherical cysticus in the wild quickly and accurately, resulting in early warning and monitoring of red tides.
Method used
A molecular marker pgcp1 based on the chloroplast genome was designed, and specific primers and probes were developed, combined with recombinase polymerase amplification (RPA) reaction and lateral flow strip (LFD) detection method to achieve rapid quantitative detection of cystosus spherical cystosus.
Benefits of technology
The specific detection of spherical cystosus is achieved, with high sensitivity and detection limits below 4 cells/mL. It can quickly conduct quantitative analysis in the field, supporting early red tide monitoring and early warning.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention belongs to the field of marine environmental protection, and particularly relates to a specific primer, a system, application, a kit and a detection method for detecting Paleocystis globosa. The invention discloses a specific primer for detecting globocystis globosa, and the specific primer comprises an upstream primer pgcp1-10F 5 'TCAATTTTCTGTTACAATTTATARTGAG 3' and a downstream primer pgcp1-10R 5 'CTYTAATRCTAAGTMAATCTTTTACAAC 3'. The specific primer and a system provided by the invention can be used for qualitatively and quantitatively detecting and monitoring offshore globocystis globosa in China, and have the advantages of high specificity, high sensitivity, simplicity and convenience in operation, rapidness in detection, wide detection range and the like.
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