Tissue stationary liquid and application thereof

By adopting a tissue fixing solution containing a variety of chemical substances, the problems of tissue fixing solution in the prior art resulting in water loss, shrinkage and deformation of the specimen, achieving good fixation effects on various tissue types and having economical benefits.

CN119715078APending Publication Date: 2025-03-28THE FIRST AFFILIATED HOSPITAL OF BENGBU MEDICAL COLLEGE
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Patent Information

Application Number
CN202510153085.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-12
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

The existing tissue fixation fluid can easily cause the specimen to lose water, shrinkage and deformation during long-term storage, and is costly and has poor fixation effect, making it difficult to apply to various tissue types.

Method used

A tissue fixation solution including glyoxylic acid, formalin, anhydrous ethanol, diethoxymethane, ethyl acetate, Tween 80, acetic acid, pure water, stearic acid, polyvinyl alcohol and sodium chloride was prepared through specific ratio and mixing steps. It can be fixed at a temperature of 34~40°C for 5~10 days, significantly improving the fixation effect of various tissues.

Benefits of technology

The fixation solution can fix a variety of tissue types in humans and animals at the same time, including fat, eyeballs and bones, significantly improve the fixation effect of tissue, reduce wrinkles during paraffin sectioning, maintain the integrity of the tissue structure, have good economic effects, and can preserve histomorphological characteristics for a long time.

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Abstract

The invention belongs to the field of pathological reagents, and particularly relates to a tissue stationary liquid and application thereof. The technical problem to be solved by the invention is to provide the multipurpose tissue stationary liquid which is ready to use and has a good fixing effect. According to the technical scheme, the tissue stationary liquid is prepared from the following substances in parts by weight: 50 to 150 parts of glyoxylic acid, 50 to 150 parts of formalin, 200 to 400 parts of absolute ethyl alcohol, 200 to 400 parts of diethoxymethane, 50 to 150 parts of ethyl acetate, 50 to 150 parts of Tween 80, 20 to 150 parts of acetic acid, 200 to 400 parts of pure water, 1 to 4 parts of stearic acid, 1 to 3 parts of polyvinyl alcohol and 2 to 6 parts of sodium chloride. The main purpose of the invention is to provide a ready-to-use multi-purpose tissue stationary liquid with a good fixing effect, and the tissue stationary liquid can be used for pathological tissues and animal tissues of a human body at the same time.
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