CRM197 protein specificity neutralization detection method based on high expression
By adding iron ions to YC culture medium, the specific neutralization of CRM197 protein is achieved by using the immune complex precipitation line, which solves the problems of long detection window period and poor repetition in the existing detection methods, and achieves the effects of short detection cycle, high sensitivity and good repetition.
CN119716089AActive Publication Date: 2025-03-28浙江毓昌生物技术有限公司
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Patent Information
- Application Number
- CN202411953482.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-27
- Publication Date
- 2025-03-28
- Estimated Expiration
- 2044-12-27
AI Technical Summary
Technical Problem
The existing CRM197 protein-specific neutralization detection methods have problems such as long detection window period, poor repetition and insufficient specific neutralization phenomenon.
Method used
By adding iron ions to YC culture medium, Corynebacterium diphtheria accelerates the secretion of specific proteins, and uses highly expressed specific proteins to form an immune complex precipitation line with diphtheria antitoxin to achieve specific neutralization.
Benefits of technology
This method simplifies the detection process, shortens the detection cycle, improves the sensitivity and repetition of the detection, and the phenomenon is obvious and economical.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses a CRM197 protein specificity neutralization detection method based on high expression, and belongs to the technical field of biological detection. Comprising the following steps: S1, redissolving a strain: redissolving a corynebacterium diphtheriae strain to obtain a bacterial suspension; s2, bacterial liquid culture: inoculating the bacterial suspension into a YC liquid culture medium containing ferric trichloride for culture to obtain a bacterial liquid; and S3, specific neutralization: inoculating an Elek's culture medium with the bacterial liquid, and carrying out virulence determination. A novel CRM197 protein specificity neutralization detection method is established, the technical requirements for the detection effect and the time limit are greatly improved, and a new detection means is provided for biological science.
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