A feed additive for strengthening the intestine and protecting the liver of poultry and its application

Abstract

The present invention provides a feed additive for intestinal health and liver protection of poultry and its application, belonging to the technical field of livestock and poultry feed. The present invention provides a feed additive, which comprises, by weight: 10 to 20 parts of Saccharomyces boulardii, 16 to 32 parts of a composite antioxidant and 50 to 100 parts of a mycotoxin adsorbent; the composite antioxidant comprises glucose oxidase, yeast selenium and plant polyphenols. The feed additive can improve the intestinal tract and / or liver health of poultry, promote poultry growth, increase feed conversion rate, and reduce mortality rate; it can extend the peak egg-laying period of laying poultry, increase egg production rate, reduce feed-egg ratio, and improve egg quality; it can increase the healthy chick rate of breeding poultry, etc. Further, the feed additive can also improve the feeding effect of moldy diets and alleviate the intestinal and liver damage caused by moldy diets.

Description

Technical Field

[0001] The invention belongs to the technical field of livestock and poultry feed, and particularly relates to a feed additive for strengthening the intestine and protecting the liver of poultry and an application thereof. Background Art

[0002] The intestine is the main site of digestion and absorption in poultry, and is also the largest immune organ in the body. The intestine plays an important barrier function, and its health is a key factor in determining poultry production performance. A healthy intestine can prevent harmful substances in the intestinal cavity (such as bacteria and various toxins) from passing through the intestinal mucosa and entering other tissues and organs and the blood circulation system in the body; when the intestinal barrier is damaged, pathogenic microorganisms and toxins in the intestine will enter the blood circulation, causing necrotic enteritis, watery stools and feed stools.

[0003] The liver is the main site for fatty acid oxidation and decomposition, and synthesis of lipoprotein and yolk precursors in poultry. There is a two-way interaction between the intestine and its microorganisms and the liver. Unhealthy intestines, unbalanced diets, bacteria, toxins and improper feeding management can all cause liver damage, leading to hepatitis and fatty liver, and even liver rupture, thereby reducing the immunity and production performance of poultry and increasing the mortality rate of poultry.

[0004] In addition, there are various stress factors in poultry production, such as excessive ovulation in the late egg-laying period leading to ovarian oxidative stress; dietary factors such as the presence of toxic metals, mycotoxin contamination in feed, fat oxidation in feed, environmental factors such as too high or too low temperature in the poultry house, insufficient light and ventilation, as well as chick transportation, vaccine immunization, etc. can cause varying degrees of oxidative stress in poultry, reducing poultry production performance and product quality.

[0005] Different degrees of oxidative stress in poultry often affect the normal functions of the intestines and liver, which in turn has an adverse effect on the growth, development, and egg production of poultry. Summary of the invention

[0006] In view of the defects in the prior art, the object of the present invention is to provide a feed additive, which has the effects of strengthening the intestine and protecting the liver, can improve the state of poultry breeding from different aspects, promote poultry growth and development and egg production, etc.

[0007] The objective of the present invention is achieved through the following technical solutions:

[0008] The present invention provides a feed additive, which comprises, by weight:

[0009] 10-20 parts of Saccharomyces boulardii, 16-32 parts of composite antioxidants and 50-100 parts of mycotoxin adsorbents.

[0010] Preferably, the composite antioxidant comprises glucose oxidase, yeast selenium and plant polyphenols.

[0011] Preferably, the composite antioxidant comprises, by weight: 1-2 parts of glucose oxidase, 10-20 parts of yeast selenium and 5-10 parts of plant polyphenols.

[0012] Preferably, the enzymatic activity of the glucose oxidase is ≥50000 IU / g.

[0013] The present invention provides a method for preparing the feed additive described in the above technical solution, comprising the following steps:

[0014] The boulardii saccharomyces, the composite antioxidant and the mycotoxin adsorbent are mixed according to parts by mass to obtain a feed additive.

[0015] The present invention provides application of the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution in poultry breeding.

[0016] Preferably, the application includes any one or two or more of the following (1) to (8):

[0017] (1) Improve the intestinal and / or liver health of poultry;

[0018] (2) Extend the peak egg-laying period and increase egg-laying rate;

[0019] (3) Improve egg quality;

[0020] (4) Improve the rate of healthy chicks;

[0021] (5) Reduce the mortality rate;

[0022] (6) Reduce feed-to-egg ratio;

[0023] (7) Promote poultry growth;

[0024] (8) Improve feed conversion efficiency.

[0025] The present invention provides the use of the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution in improving the adverse effects of harmful components in the diet on poultry.

[0026] The present invention provides a poultry feed, comprising the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution; the poultry feed also includes a basic daily diet.

[0027] The present invention provides a method for feeding poultry, comprising:

[0028] Adding the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution into the diet to obtain a mixed feed;

[0029] The mixed feed is used to feed poultry.

[0030] Beneficial effects of the present invention:

[0031] The present invention provides a feed additive, which comprises, by weight, 10 to 20 parts of Saccharomyces boulardii, 16 to 32 parts of a composite antioxidant, and 50 to 100 parts of a mycotoxin adsorbent. In the feed additive provided by the present invention, Saccharomyces boulardii has a strong oxygen consumption capacity and the advantage of producing special proteases, can adhere to Escherichia coli and Salmonella, decompose Clostridium toxins and Escherichia coli endotoxins, promote the proliferation of beneficial bacteria, and balance intestinal flora; it can also effectively reduce the negative effects of mycotoxins; the Saccharomyces boulardii can also enhance the tight junctions between epithelial cells and enhance resistance to harmful substances by upregulating some genes; the Saccharomyces boulardii can also improve the body's immune and anti-inflammatory functions, thereby improving the body's disease resistance, etc. The composite antioxidant composed of glucose oxidase, yeast selenium and plant polyphenols can maintain the redox balance in poultry and enhance the anti-stress ability of poultry. The mycotoxin adsorbent, Saccharomyces boulardii and composite antioxidants work together to further reduce the negative effects of mycotoxins, so that the feed additives can exert a stronger combined effect and achieve the effect of strengthening the intestine and protecting the liver. The feed additives provided by the present invention can improve the health of the intestines and / or livers of poultry, prolong the peak egg-laying period of poultry, increase the egg production rate, improve the egg quality, increase the healthy chick rate, reduce the mortality rate and feed-egg ratio, promote poultry growth and improve feed conversion rate. The results of the embodiments show that the feed additives provided by the present invention can improve the hatching rate of broiler breeders, reduce the content of serum endotoxins and interleukin IL-6 in broiler breeders, reduce the activity of alanine aminotransferase and aspartate aminotransferase, and reduce the mortality rate of broiler breeders. The feed additives can improve the egg production rate and feed-egg ratio of laying hens, improve egg quality, reduce the number of Escherichia coli and Salmonella in the cecum of laying hens; increase the activity of the intestinal and liver antioxidant enzyme superoxide dismutase SOD, and reduce the content of malondialdehyde MDA, a product of lipid peroxidation in the intestine and liver. Furthermore, the feed additive can also alleviate the adverse effects of moldy diets on the growth performance, intestinal tract and liver health of broiler chickens. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0033] Figure 1 This is a statistical result chart of the mortality rate of broiler breeder chickens in each test group in Application Example 1;

[0034] Figure 2 This is a graph showing the detection results of the number of Escherichia coli and Salmonella in the cecum of laying hens in each test group in Application Example 2;

[0035] Figure 3 The results of superoxide dismutase (SOD) activity test of the intestinal tract and liver of laying hens in each test group in Application Example 2 are shown in FIG.

[0036] Figure 4 The figure is the test result of malondialdehyde (MDA) content in the intestine and liver of laying hens in each test group in Application Example 2;

[0037] Figure 5 This is a graph showing the intestinal and liver index test results of broiler chickens in each test group in Application Example 3. DETAILED DESCRIPTION

[0038] The present invention provides a feed additive, which comprises, by weight:

[0039] 10-20 parts of Saccharomyces boulardii, 16-32 parts of composite antioxidants and 50-100 parts of mycotoxin adsorbents.

[0040] Unless otherwise specified, the present invention has no particular limitation on the sources of the raw materials, and any conventional commercially available product in the art may be used.

[0041] The feed additive provided by the present invention includes 10 to 20 parts of Saccharomyces boulardii, preferably 18 to 20 parts, and more preferably 20 parts by weight. In the present invention, the Saccharomyces boulardii preferably includes Levucell, a commercial product produced by Raman Group of Canada. In the present invention, the bradychium regulates intestinal flora by means of rapid oxygen consumption, adhesion to pathogens and decomposition of toxins, and improves intestinal health by means of enhancing intestinal barrier function and immune function; the bradychium is the first and only probiotic authorized by the European Union in 2017 that can be used to reduce poultry Salmonella and Campylobacter infection, thereby ensuring poultry food safety. There are many factors that cause liver damage in the poultry production process. Among them, liver damage caused by pathogenic microorganisms or toxins is the most common. The bradychium can produce specific enzymes to decompose toxins produced by Clostridium, and alleviate the damage caused by vomitoxin to organs such as the intestines, liver and kidneys, comprehensively protecting the intestines and liver, and improving the health of poultry.

[0042] Based on the mass fraction of Saccharomyces boulardii, the feed additive provided by the present invention comprises 16 to 32 parts of a composite antioxidant. In the present invention, the composite antioxidant preferably comprises glucose oxidase, yeast selenium and plant polyphenols.

[0043] In the present invention, based on the mass fraction of Saccharomyces boulardii, the added amount of the glucose oxidase is preferably 1 to 2 parts, more preferably 1.5 to 1.8 parts. In the present invention, the enzymatic activity of the glucose oxidase is preferably ≥50000 IU / g, more preferably 50000 IU / g. In the present invention, the glucose oxidase is an aerobic dehydrogenase that can catalyze the dehydrogenation of β-D-glucose to generate gluconolactone and hydrogen peroxide, and gluconolactone will be converted into gluconic acid; gluconic acid can lower the intestinal pH, inhibit the growth of harmful bacteria, and promote the growth of beneficial bacteria; hydrogen peroxide has a broad-spectrum bactericidal effect, and can also stimulate beneficial bacteria to produce catalase and eliminate free radicals in the body.

[0044] In the present invention, based on the mass of Saccharomyces boulardii, the added amount of the yeast selenium is preferably 10 to 20 parts, more preferably 15 to 16 parts. In the present invention, the yeast selenium preferably includes methionine selenium and cysteine ​​selenium; the mass of methionine selenium in the yeast selenium preferably accounts for ≥63% of the mass of yeast selenium, more preferably 63%; the mass of cysteine ​​selenium in the yeast selenium preferably accounts for ≥17% of the mass of yeast selenium, more preferably 17%. Selenium is a component of glutathione peroxidase, and yeast selenium is organic selenium fermented by specially selected yeast. The main function of yeast selenium in the present invention is to further remove oxygen free radicals in the body, and the remaining yeast selenium is stored in the poultry in the form of selenoprotein as a selenium reserve or to produce selenium-rich products. The present invention does not specifically limit the source of the yeast selenium, and conventional commercial products in the field can be used.

[0045] In the present invention, based on the mass fraction of Saccharomyces boulardii, the added amount of the plant polyphenols is preferably 5 to 10 parts, more preferably 7.5 to 10 parts. In the present invention, the plant polyphenols preferably include any one or more of American chestnut leaf extract, tannic acid and lemon extract, more preferably include American chestnut leaf extract, tannic acid and lemon extract. In the present invention, if the plant polyphenols include American chestnut leaf extract, tannic acid and lemon extract; the mass ratio of the American chestnut leaf extract, tannic acid and lemon extract is preferably (70 to 80): (18 to 22): (4 to 6). In the present invention, the plant polyphenols are purchased from Silva Group, Italy. Plant polyphenols, as a kind of plant secondary metabolite, exist in many plants and fruits. Its basic structure is a phenolic ring structure with one or more hydroxyl groups that does not contain nitrogen. It has an antioxidant effect of scavenging free radicals due to its phenolic hydroxyl structure. The invention additionally adds a secondary antioxidant composed of a variety of plant polyphenols, which can completely remove various free radicals, thereby alleviating the damage caused by different free radicals to biological molecules and tissues and organs, improving the antioxidant capacity of poultry, and enhancing the resistance of poultry to various physiological and external stresses.

[0046] Based on the mass fraction of Saccharomyces boulardii, the feed additive provided by the present invention includes 50 to 100 parts of mycotoxin adsorbent, preferably 52 to 74 parts, more preferably 53 to 57 parts, and more preferably 53.5 to 56.5 parts. Feed mycotoxin contamination is one of the important risk factors that endanger animal husbandry production. Mycotoxins can directly damage target organs such as the liver and intestinal mucosa, cause immunosuppression, and seriously endanger the health of poultry. The mycotoxin adsorbent added by the present invention forms a stable complex with the toxin by means of hydrophobic binding, electrostatic attraction, hydrogen bond coordination, etc., thereby reducing the bioavailability of the toxin and reducing the content of toxins in the animal. The present invention uses the mycotoxin adsorbent as a raw material, and coordinates with Saccharomyces boulardii and a composite antioxidant to further exert a stronger combined effect, thereby achieving the effect of strengthening the intestine and protecting the liver.

[0047] The feed additive provided by the present invention is suitable as a complete feed formula for all stages of all poultry breeds. In the present invention, the poultry preferably includes any one or more of laying poultry, meat poultry and breeding poultry; the types of poultry preferably include any one or more of chicken, duck and goose. The feed additive provided by the present invention can improve the production performance and hatching performance of poultry; reduce the activity of poultry serum endotoxin, interleukin IL-6, alanine aminotransferase and aspartate aminotransferase; reduce the mortality rate of poultry; improve the egg production rate and feed-egg ratio of laying poultry, and improve egg quality; reduce the number of Escherichia coli and Salmonella in the cecum of poultry, increase the activity of superoxide dismutase SOD, an antioxidant enzyme in the intestine and liver, and reduce the content of malondialdehyde MDA, a product of lipid peroxidation in the intestine and liver. Furthermore, adding the feed additive to the moldy diet can also improve the feeding effect of the moldy diet and improve the development and health of the intestine and liver.

[0048] The present invention provides a method for preparing the feed additive described in the above technical solution, comprising the following steps:

[0049] The Saccharomyces boulardii, the composite antioxidant and the mycotoxin adsorbent are mixed according to parts by mass to obtain a feed additive. The present invention has no special limitation on the mixing method, and any conventional mixing method in the art can be used.

[0050] The present invention provides the use of the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution in poultry farming. In the present invention, the poultry preferably includes any one or more of breeding poultry, laying poultry and meat poultry; the type of poultry preferably includes any one or more of chicken, duck and goose. In the present invention, the application preferably includes any one or more of the following (1) to (8):

[0051] (1) Improve the intestinal and / or liver health of poultry;

[0052] (2) Extend the peak egg-laying period and increase egg-laying rate;

[0053] (3) Improve egg quality;

[0054] (4) Improve the rate of healthy chicks;

[0055] (5) Reduce the mortality rate;

[0056] (6) Reduce feed-to-egg ratio;

[0057] (7) Promote poultry growth;

[0058] (8) Improve feed conversion efficiency.

[0059] The feed additive provided by the present invention can improve the health of the intestine and / or liver of poultry. In the present invention, the feed additive helps to improve the antioxidant capacity of the intestine and liver of poultry and alleviate oxidative damage to the intestine and liver. The present invention shows through the results of embodiments that the feed additive fed to meat-breeding poultry can reduce the content of serum endotoxin and IL-6, as well as the activity of serum alanine aminotransferase and aspartate aminotransferase. The feed additive fed to laying poultry can increase the activity of the intestinal and liver antioxidant enzyme superoxide dismutase SOD, reduce the content of malondialdehyde MDA, a product of lipid peroxidation in the intestine and liver, and reduce the number of cecal Escherichia coli and Salmonella. In the present invention, the feed additive is added to the moldy diet for feeding meat poultry aged 1 to 42 days, which can reduce the intestinal index and liver index of meat poultry and improve the development and health of the intestine and liver.

[0060] The feed additive provided by the present invention can prolong the peak period of egg laying and improve the egg laying rate. The present invention shows through the results of the examples that the feed additive is added to the basic diet for feeding laying poultry, which can improve the egg laying rate of laying poultry.

[0061] The feed additive provided by the present invention can improve the quality of poultry eggs. The present invention shows through the results of the examples that the feed additive is added to the basic diet for feeding laying poultry, which can improve the quality of eggs, increase the eggshell strength and eggshell thickness, and increase the average egg weight.

[0062] The feed additive provided by the present invention can improve the healthy chick rate. The present invention shows through the results of the examples that the feed additive is added to the basic daily diet for feeding breeding poultry, which can improve the healthy chick rate of breeding poultry.

[0063] The feed additive provided by the present invention can reduce the mortality rate. The present invention shows through the results of the examples that the feed additive is added to the basic daily diet for feeding breeding poultry, which can reduce the mortality rate of breeding poultry.

[0064] The feed additive provided by the present invention can reduce the feed-to-egg ratio. The present invention shows through the results of the examples that the feed additive is added to the basic diet for feeding laying poultry, which can reduce the feed-to-egg ratio.

[0065] The feed additive provided by the present invention can promote the growth of poultry. The present invention shows through the results of examples that the feed additive is added to the basic daily diet for feeding meat poultry, which can increase the average daily weight gain of meat poultry.

[0066] The feed additive provided by the present invention can improve feed conversion efficiency. The present invention shows through the results of the examples that the feed additive can reduce the feed-to-meat ratio of meat poultry and improve feed conversion efficiency.

[0067] The present invention provides the use of the feed additive described in the above technical scheme or the feed additive prepared by the preparation method described in the above technical scheme in improving the adverse effects of harmful components in the diet on poultry. In the present invention, the harmful components preferably include mycotoxins. The present invention adds the feed additive to the moldy diet and feeds one-day-old broilers. The results show that, compared with feeding only the moldy diet, adding the feed additive to the moldy diet can increase the average daily weight gain and average daily feed intake of broilers, reduce the feed-to-meat ratio of broilers, and achieve growth performance similar to that of broilers fed with a non-moldy basic diet; adding the feed additive to the moldy diet can reduce the intestinal index and liver index of broilers, and improve intestinal and liver health.

[0068] The present invention provides a poultry feed, comprising the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution; the poultry feed also includes a basic daily diet.

[0069] The present invention has no special limitation on the source of the basic diet, and any conventional basic diet in the art can be used. In the present invention, the amount of feed additive added to the poultry feed is preferably 0.05wt.% to 0.2wt.%, more preferably 0.1wt.% of the basic diet.

[0070] The present invention also provides a method for feeding poultry, comprising:

[0071] Adding the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution to the diet to obtain a mixed feed;

[0072] The mixed feed is used to feed poultry.

[0073] In the present invention, the addition amount of the feed additive is preferably 0.05wt.% to 0.2wt.%, more preferably 0.1wt.%. After the mixed feed is obtained, the present invention has no special limitation on the feeding method of the mixed feed, and any conventional mixing method in the art can be used.

[0074] The present invention adds the feed additive to the basic diet to feed poultry, which can improve the adverse effects of harmful components in the diet on poultry, improve the intestinal tract and / or liver health of poultry, promote poultry growth, etc. In the embodiment of the present invention, the feed additive is added to the moldy diet for feeding 1-42 days old meat poultry, which can reduce the intestinal index and liver index of meat poultry, improve the development and health of the intestine and liver; adding the feed additive to the moldy diet can also improve the average daily weight gain and average daily feed intake of broilers, reduce the feed-to-meat ratio of broilers, and achieve growth performance similar to that of broilers fed with non-moldy basic diets.

[0075] In order to further illustrate the present invention, the technical solution provided by the present invention is described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0076] In the following examples, the source of Brady is Brady The activity of glucose oxidase is not less than 50000 IU / g; the yeast selenium contains not less than 63% of methionine selenium and 17% of cysteine ​​selenium by mass; the plant polyphenols are purchased from Silva Company of Italy.

[0077] Example 1

[0078] A feed additive, calculated by weight, consisting of:

[0079] Brady Levucell 20 parts, glucose oxidase 1.5 parts, yeast selenium 15 parts, plant polyphenols 10 parts and mycotoxin adsorbent 53.5 parts.

[0080] The preparation method of feed additive is as follows:

[0081] The above components can be uniformly mixed according to mass parts.

[0082] Example 2

[0083] A feed additive, calculated by weight, consisting of:

[0084] Brady Levucell 18 parts, glucose oxidase 1.8 parts, yeast selenium 16 parts, plant polyphenols 7.5 parts and mycotoxin adsorbent 56.7 parts.

[0085] The preparation method of the feed additive is the same as that in Example 1.

[0086] Example 3

[0087] A feed additive, calculated by weight, consisting of:

[0088] Brady Levucell 10 parts, 1 part of glucose oxidase, 10 parts of yeast selenium, 5 parts of plant polyphenols and 74 parts of mycotoxin adsorbent.

[0089] The preparation method of the feed additive is the same as that in Example 1.

[0090] Comparative Example 1

[0091] A feed additive, calculated by weight, consisting of:

[0092] 1.5 parts of glucose oxidase, 15 parts of yeast selenium, 10 parts of plant polyphenols and 73.5 parts of mycotoxin adsorbent.

[0093] The preparation method of the feed additive is the same as that in Example 1.

[0094] Comparative Example 2

[0095] A feed additive, calculated by weight, consisting of:

[0096] Brady Levucell 20 parts, 1.5 parts of glucose oxidase, 15 parts of yeast selenium and 63.5 parts of mycotoxin adsorbent.

[0097] The preparation method of the feed additive is the same as that in Example 1.

[0098] Comparative Example 3

[0099] A feed additive, calculated by weight, consisting of:

[0100] Brady Levucell 8 parts, glucose oxidase 3 parts, yeast selenium 25 parts, plant polyphenols 3 parts and mycotoxin adsorbent 61 parts.

[0101] Application Example 1

[0102] Verify the feeding effect of the feed additives of Examples 1 to 3 and Comparative Examples 1 to 3 on broiler breeder chickens. This test is conducted on the basis of the feed additives for intestinal health and liver protection of poultry in Examples 1 to 3 and Comparative Examples 1 to 3, and the test animals are broiler breeder chickens:

[0103] In June 2022, a field test of the above-mentioned feed additive was conducted at a large breeding chicken farm in Hebei. The test selected 1,296 41-week-old Ross 308 broiler breeders and randomly divided them into 9 groups, namely, test group 1, test group 2, test group 3, control group 1, control group 2, control group 3, control group 4, control group 5 and blank control. Among them, test group 1, test group 2, test group 3, control group 1, control group 2 and control group 3 added 0.1wt% of the feed additive of Example 1, 0.1wt% of the feed additive of Example 2, 0.1wt% of the feed additive of Example 3, 0.1wt% of the feed additive of Comparative Example 1, 0.1wt% of the feed additive of Comparative Example 2 and 0.1wt% of the feed additive of Comparative Example 3 in the basic diet formula, and 0.02wt% of Brady Levucell was added to the basic diet in the control group 4. The control group 5 was fed with 0.01wt% plant polyphenols in the basic diet, and the blank control group was fed with the basic diet. Each group had 6 replicates, and each replicate had 24 chickens. The pre-test period was 1 week, and the main test period was 12 weeks. During the trial, the broiler breeders had free access to food and water, and were given 16 hours of light per day. The immunization and disinfection procedures were carried out in accordance with the farm management regulations.

[0104] During the experiment, the number of eggs laid and the number of deaths and culls of broiler breeder hens were recorded every day in replicates, and the egg laying rate and mortality rate were calculated; on the 18th day after incubation, the eggs were candled, the number of fertilized eggs was recorded, and the fertilization rate of the eggs was calculated (the number of fertilized eggs / the number of eggs incubated × 100%); on the 21st day after incubation, the chicks were hatched, the number of hatched chicks and the number of healthy chicks were recorded, and the hatching rate of the incubated eggs (the number of hatched eggs / the number of eggs incubated × 100%) and the rate of healthy chicks (the number of healthy chicks / the number of hatched chicks × 100%) were calculated; at the end of the experiment, 2 chickens were selected from each replicate to collect blood, separate serum, and determine the levels of serum endotoxin and interleukin IL-6, as well as the activities of alanine aminotransferase and aspartate aminotransferase.

[0105] 1. The production performance and hatching performance results of broiler breeders in each experimental group are shown in Table 1.

[0106] Table 1 Production performance and hatching performance results of broiler breeders in each experimental group

[0107]

[0108]

[0109] As shown in Table 1, the basal diet was supplemented with the poultry intestinal health and liver protection feed additives described in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2 and Comparative Example 3, and the basal diet was supplemented with Levucell and plant polyphenols can improve the production performance and hatching performance of broiler breeders; among them, compared with the blank control group, the basal diet supplemented with 0.1wt% of the feed additive of Example 1 has the best effect on improving the production performance and hatching performance of broiler breeders, which can increase the egg production rate by 1.83%, the fertilization rate by 2.19%, the hatching rate by 1.56%, and the healthy chick rate by 1.35%. Combined with the improvement of egg production rate, fertilization rate, hatching rate and healthy chick rate, within the 12-week test period, broiler breeders fed with the basal diet supplemented with 0.1wt% of the feed additive of Example 1 can produce 3.6 more chicks per chicken on average, and earn 13.68 yuan more.

[0110] 2. The results of serum biochemical indexes of broiler breeder chickens in each experimental group are shown in Table 2.

[0111] Table 2 Results of serum biochemical indexes of broiler breeders in each experimental group

[0112]

[0113] As shown in Table 2, the basal diet was supplemented with the feed additives described in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2 and Comparative Example 3, and the basal diet was supplemented with Levucell and plant polyphenols can improve the biochemical indexes of broiler breeder serum; among them, compared with the blank control group, the basal diet supplemented with 0.1wt% of Example 1 had the lowest serum endotoxin, interleukin IL-6 content, and the activities of alanine aminotransferase and aspartate aminotransferase, indicating that the intestines and livers of broiler breeders were the healthiest and the inflammation level was the lowest. Because endotoxin is an indicator of intestinal integrity, the lower the content, the better the intestinal integrity; the activities of alanine aminotransferase and aspartate aminotransferase are indicators of liver function, the lower the content, the better the liver function.

[0114] The statistical results of the mortality rate of broiler breeders in each experimental group are as follows: Figure 1 shown. Figure 1 The vertical axis is the mortality rate (%).

[0115] Depend on Figure 1 It can be seen that the basal diet is supplemented with the feed additives described in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2 and Comparative Example 3, and the addition of Brady Levucell alone Both the plant polyphenols and the basal diet can reduce the mortality rate of broiler breeders; among them, compared with the blank control group, the basal diet supplemented with 0.1wt% of Example 1 had the lowest mortality rate of broiler breeders and the best effect of reducing the mortality rate of broiler breeders, reducing the mortality rate by 1.8%.

[0116] Application Example 2

[0117] Feeding effect of the feed additive prepared in Example 1 on laying hens

[0118] In March 2023, a field test of the above feed additive was conducted in a large laying hen farm in Hubei. The test selected 800 healthy Hailan brown laying hens aged 60 weeks and randomly divided them into 4 groups, namely the control group, test group 1, test group 2 and test group 3, wherein the control group was fed with a basic diet, and test groups 1, 2 and 3 were respectively added with 0.05wt%, 0.1wt% and 0.2wt% of the feed additive prepared in Example 1 in the basic diet. There were 10 replicates in each group, 20 chickens in each replicate, a pre-feeding period of 1 week, and a formal test period of 8 weeks.

[0119] During the experiment, the number of eggs laid, egg breakage rate, egg weight and feed intake were recorded every day, and the egg production rate, feed-egg ratio and average daily egg weight were calculated. At the end of the experiment, two chickens were selected from each replicate to collect jejunal, cecal chyme and liver, and the antioxidant indexes of jejunal and liver, the number of Escherichia coli and Salmonella in cecal chyme, and egg quality related indicators were determined. During the experiment, laying hens were free to eat and drink, and were given 16 hours of light every day. Immunization and disinfection procedures were carried out in accordance with farm management regulations.

[0120] 1. The production performance test results of laying hens in each test group are shown in Table 3.

[0121] Table 3 Production performance test results of laying hens in each test group

[0122] Control group Test Group 1 Test Group 2 Experiment 3 groups Egg production rate (%) 81.92 82.47 83.18 82.90 Egg breaking rate (%) 1.12 0.88 0.47 0.63 Average daily feed intake (g / d) 118.24 118.31 118.67 118.35 Average egg weight / (g / egg) 62.11 62.50 63.05 62.68 Feed to Egg Ratio 2.32 2.30 2.26 2.28

[0123] As can be seen from Table 3, adding 0.05wt%, 0.1wt% and 0.2wt% of the feed additive described in Example 1 to the basal diet can improve the egg production rate and feed-egg ratio of laying hens, reduce the egg breakage rate, and improve the quality of eggs; among them, adding 0.1wt% of the feed additive described in Example 1 to the basal diet has the best effect on improving the production performance of laying hens, which can increase the egg production rate by 1.26%, reduce the egg breakage rate by 0.65%, and reduce the feed-egg ratio by 0.06. Combined with the improvements in egg production rate, egg breakage rate and feed-egg ratio, during the 8-week test period, laying hens fed with 0.1wt% of the feed additive described in Example 1 added to the basal diet can produce one more egg per chicken, eat 0.176kg less feed, and earn 1.12 yuan more.

[0124] 2. The egg quality test results of laying hens in each test group are shown in Table 4.

[0125] Table 4 Egg quality test results of laying hens in each test group

[0126] Control group Test Group 1 Test Group 2 Experiment 3 groups Eggshell thickness (mm) 0.32 0.34 0.38 0.37 Eggshell strength (N) 38.80 40.28 41.65 40.48 Egg shape index 1.33 1.34 1.35 1.34 Yolk color 5.84 5.89 5.96 5.92 Haugh unit 81.92 82.65 83.84 83.35

[0127] As shown in Table 4, adding 0.05wt%, 0.1wt% and 0.2wt% of the feed additive described in Example 1 to the basic diet can improve egg quality; among them, adding 0.1wt% of the feed additive described in Example 1 to the basic diet has the best effect on improving egg quality, which is mainly reflected in improving the quality of eggshell and egg white, such as improving eggshell strength and eggshell strength, as well as Haugh unit.

[0128] 3. The test results of Escherichia coli and Salmonella in the cecum of laying hens in each test group are as follows: Figure 2 And as shown in Table 5. Figure 2 The vertical axis is the number of cecal microorganisms (Log 10 (Copy number / g chyme)

[0129] Table 5 Detection results of Escherichia coli and Salmonella in the cecum of laying hens in each experimental group

[0130] Control group Test Group 1 Test Group 2 Experiment 3 groups <![CDATA[Escherichia coli (Log 10 (copy number / g of digesta))]]> 7.53 7.28 6.75 7.06 <![CDATA[Salmonella (Log 10 (copy number / g of chyme))]]> 6.37 6.16 5.82 5.98

[0131] From Table 5 and Figure 2 It can be seen that adding 0.05wt%, 0.1wt% and 0.2wt% of the feed additive described in Example 1 to the basic diet can reduce the number of cecal Escherichia coli and Salmonella, among which adding 0.1wt% of the feed additive of Example 1 to the basic diet has the best effect on reducing intestinal pathogens.

[0132] 4. The results of antioxidant indexes of the intestinal tract and liver of laying hens in each test group are shown in Tables 6-7 and Figures 3-4 As shown. Among them, Figure 3 The results of superoxide dismutase (SOD) activity test in the intestine and liver of laying hens in each test group are shown in the figure;

[0133] Figure 4 The following is the test result of malondialdehyde (MDA) content in the intestine and liver of laying hens in each test group. Figure 3 The vertical axis is the activity of SOD in the intestine and liver of laying hens (U / mg); Figure 4 The vertical axis represents the content of malondialdehyde (MDA) in the intestine and liver of laying hens (mmol / mg).

[0134] Table 6 Results of superoxide dismutase (SOD) activity test in the intestine and liver of laying hens in each test group

[0135]

[0136]

[0137] Table 7 Detection results of malondialdehyde (MDA) content in the intestine and liver of laying hens in each test group

[0138] jejunum liver Control group 2.14 2.36 Test Group 1 2.06 2.24 Test Group 2 1.83 2.05 Experiment 3 groups 1.92 2.12

[0139] From Tables 6 to 7 and Figures 3-4 It can be seen that adding 0.05wt%, 0.1wt% and 0.2wt% of the feed additives described in Example 1 to the basic diet can improve the activity of the antioxidant enzyme superoxide dismutase SOD in the intestine and liver, and reduce the content of malondialdehyde MDA, a product of lipid peroxidation in the intestine and liver. Among them, adding 0.1wt% of the feed additives of Example 1 to the basic diet has the best effect of improving the antioxidant capacity of the intestine and liver of laying hens and alleviating oxidative damage to the intestine and liver. It shows that the feed additives provided by the present invention are helpful to improve the antioxidant capacity of the intestine and liver of laying hens, alleviate oxidative damage to the intestine and liver, reduce the incidence of liver and intestinal diseases in laying hens, and thus reduce the use of antibiotics on the breeding site and improve production efficiency.

[0140] Application Example 3

[0141] Experimental study on the effect of the feed additive prepared in Example 1 on improving the feeding of moldy diets

[0142] The experiment was conducted in a large broiler farm in Shandong in August 2023. The experiment selected 384 one-day-old AA broilers and randomly divided them into 8 groups, namely the control group, negative control group, test group 1, test group 2, test group 3, test group 4, test group 5 and test group 6. Among them, the control group was fed with a basic diet that did not contain moldy corn; the negative control group was fed with a moldy corn diet (where the moldy corn diet was 50% of the corn in the basic diet replaced with naturally moldy corn, the same below); the test group 1 added 0.02wt% Brady Levucell to the moldy corn diet In the second test group, 0.01wt% plant polyphenols were added to the moldy corn diet; in the third test group, 0.015wt% yeast selenium was added to the moldy corn diet; in the fourth test group, 0.0015wt% glucose oxidase was added to the moldy corn diet; in the fifth test group, 0.05wt% mycotoxin adsorbent was added to the moldy corn diet; in the sixth test group, 0.1wt% feed additive of Example 1 was added to the moldy corn diet. Each group had 6 replicates, each replicate had 8 chickens, and the test period was 42 days. During the test, the broilers had free access to food and water.

[0143] During the experiment, the feed intake was recorded every day. At the end of the experiment, the chickens were weighed to calculate the average daily feed intake, average daily weight gain and feed-to-meat ratio (average daily feed intake / average daily weight gain). At the end of the experiment, 2 chickens were selected from each replicate to take the intestines and livers, and the intestinal index and liver index (intestinal or liver weight / live weight) were calculated.

[0144] 1. The results of broiler growth performance of each test group are shown in Table 8.

[0145] Table 8 Growth performance of broiler chickens in each experimental group from 1 to 42 days of age

[0146] Average daily feed intake (g / d) Average daily weight gain (g / d) Feed to meat ratio Control group 87.95 54.13 1.62 Negative control group 82.26 48.82 1.68 Test Group 1 85.02 51.54 1.65 Test Group 2 84.86 51.23 1.66 Experiment 3 groups 84.75 51.02 1.66 Test 4 groups 83.72 50.28 1.67 Test 5 groups 84.08 50.51 1.66 Experiment 6 groups 86.73 53.07 1.63

[0147] As shown in Table 8, the moldy diet reduced the average daily weight gain and average daily feed intake of broilers, increased the feed-to-meat ratio of broilers, and reduced the growth performance of broilers; while adding Brady Levucell alone to the moldy diet Plant polyphenols, yeast selenium, glucose oxidase and mycotoxin adsorbent, as well as the feed additive of Example 1 can all increase the average daily weight gain and average daily feed intake of broilers, reduce the feed-to-meat ratio of broilers, and improve the growth performance of broilers; among them, Example 1 has the best effect on improving the growth performance of broilers, and can reduce the feed-to-meat ratio of broilers fed with moldy diets by 0.05. A reduction in the feed-to-meat ratio of 0.05 can increase the income of each chicken by 0.35 yuan on average, that is, the broilers fed with the feed additive of Example 1 can earn an extra 0.35 yuan per chicken.

[0148] 2. The results of intestinal and liver index tests of broiler chickens in each test group are shown in Table 9 and Figure 5 shown.

[0149] Table 9 Results of intestinal and liver index tests for broiler chickens in each experimental group

[0150] Intestinal index (%) Liver index (%) Control group 5.32 1.93 Negative control group 5.98 2.37 Test Group 1 5.46 2.09 Test Group 2 5.51 2.12 Experiment 3 groups 5.58 2.16 Test 4 groups 5.68 2.23 Test 5 groups 5.62 2.19 Experiment 6 groups 5.37 1.96

[0151] From Table 9 and Figure 5 It can be seen that the moldy diet increased the intestinal index and liver index of broilers; while adding Brady Levucell to the moldy diet alone Plant polyphenols, yeast selenium, glucose oxidase and mycotoxin adsorbent, as well as the feed additive of Example 1 can all reduce the intestinal index and liver index of broiler chickens; among them, the feed additive of Example 1 has the best effect on reducing the intestinal index and liver index of broiler chickens. The improvement of the liver and intestine by the feed additive of Example 1 can significantly reduce intestinal diseases and liver diseases of broiler chickens at the breeding site, such as broiler diarrhea, liver rupture, hepatic ascites, etc., thereby reducing the use of antibiotics and other drugs on site and further improving production efficiency.

[0152] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A feed additive, characterized in that: Calculated by mass, including: 10-20 parts of Saccharomyces boulardii, 16-32 parts of composite antioxidants and 50-100 parts of mycotoxin adsorbents.

2. The feed additive according to claim 1, characterized in that: The composite antioxidant comprises glucose oxidase, yeast selenium and plant polyphenols.

3. The feed additive according to claim 2, characterized in that: The composite antioxidant comprises, by weight, 1 to 2 parts of glucose oxidase, 10 to 20 parts of yeast selenium and 5 to 10 parts of plant polyphenols.

4. The feed additive according to claim 2 or 3, characterized in that: The enzymatic activity of the glucose oxidase is ≥50000 IU / g.

5. The method for preparing the feed additive according to any one of claims 1 to 4, characterized in that: The following steps are involved: The boulardii saccharomyces, the composite antioxidant and the mycotoxin adsorbent are mixed according to parts by mass to obtain a feed additive.

6. Use of the feed additive according to any one of claims 1 to 4 or the feed additive prepared by the preparation method according to claim 5 in poultry breeding.

7. The use according to claim 6, characterized in that: The application includes any one or more of the following (1) to (8): (1) Improve the intestinal and / or liver health of poultry; (2) Extend the peak egg-laying period and increase egg-laying rate; (3) Improve egg quality; (4) Improve the rate of healthy chicks; (5) Reduce the mortality rate; (6) Reduce feed-to-egg ratio; (7) Promote poultry growth; (8) Improve feed conversion efficiency.

8. Use of the feed additive according to any one of claims 1 to 4 or the feed additive prepared by the preparation method according to claim 5 in improving the adverse effects of harmful ingredients in the diet on poultry.

9. A poultry feed, characterized in that: The poultry feed comprises the feed additive according to any one of claims 1 to 4 or the feed additive prepared by the preparation method according to claim 5; the poultry feed also comprises a basic daily diet.

10. A method for feeding poultry, characterized in that: include: Adding the feed additive according to any one of claims 1 to 4 or the feed additive prepared by the preparation method according to claim 5 to a diet to obtain a mixed feed; The mixed feed is used to feed poultry.

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