Application of gastrodia elata polysaccharide in preparation of product for treating medium-wave ultraviolet irradiation acute photoaging skin injury

Abstract

The invention discloses application of gastrodia elata polysaccharide in preparation of a product for treating medium-wave ultraviolet (UVB) irradiation acute photoaging skin injury, and relates to the technical field of biological medicine. The gastrodia elata polysaccharide can be used for treating skin injury caused by medium-wave ultraviolet (UVB) acute photoaging, effectively improving skin pigmentation, scales, erythema, wrinkle increase, skin thickening, elasticity reduction, skin ulceration, edema and other symptoms, and has great application value.

Description

Technical Field

[0001] The invention relates to the technical field of biomedicine, and in particular to an application of gastrodia elata polysaccharide in preparing a product for treating acute photoaging skin damage caused by ultraviolet B (UVB) irradiation. Background Art

[0002] The skin is the largest organ in the human body and is the main barrier against heat loss, dehydration, mechanical trauma and microbial invasion. When the skin is exposed to external stimuli such as ionizing radiation, ultraviolet rays, burns, etc., the skin barrier is damaged and causes skin problems. Ultraviolet rays in sunlight are the most common light component exposed to the environment. According to the wavelength of ultraviolet rays (UV), UV is divided into long-wave ultraviolet rays (UVA, 320-400nm), medium-wave ultraviolet rays (UVB, 280-320nm) and short-wave ultraviolet rays (UVC, 200-280nm). Only UVA and UVB can penetrate the earth's atmosphere. UVA can penetrate the dermis due to its wavelength, while medium-wave UVB is mainly absorbed in the shallow layer and has higher energy. It has strong carcinogenicity and mutagenicity because it can be directly absorbed by DNA and form specific light damage, which has attracted widespread attention. Skin photoaging refers to the premature aging of the skin induced by ultraviolet rays (UV), which is mainly manifested as skin sagging, roughness, desquamation, pigmentation, and wrinkle formation. Photoaging is mainly caused by exposure to long-wave ultraviolet rays (UVA) and medium-wave ultraviolet rays (UVB).

[0003] According to tradition, Gastrodia elata is both a food and a Chinese medicinal material. It has multiple pharmacological effects and contains more than 200 bioactive ingredients and plant secondary metabolites, among which polysaccharides have the highest content. Studies have shown that Gastrodia elata polysaccharides have the activities of regulating immunity, anti-tumor, anti-oxidation, anti-aging, improving memory, improving cerebral ischemia, lowering blood pressure, lowering blood lipids and antibacterial.

[0004] Many previous studies have attempted to find natural products with antioxidant and anti-inflammatory properties to protect against or reduce UVB-related skin damage, but these natural products often have short durations of action and have defects such as the need for repeated application, large application areas, and uncertain effects. Summary of the invention

[0005] In order to solve the above technical problems, the purpose of the present invention is to provide a use of Gastrodia elata polysaccharide in the preparation of a product for treating acute photoaging skin damage caused by ultraviolet B (UVB) irradiation.

[0006] The technical solution of the present invention to solve the above technical problem is as follows: provide an application of Gastrodia elata polysaccharide in preparing a product for treating acute photoaging skin damage caused by medium-wave ultraviolet irradiation.

[0007] Furthermore, the Gastrodia elata polysaccharide is a Gastrodia elata extract prepared by water extraction and alcohol precipitation method.

[0008] Furthermore, the above-mentioned product is a medicine, a reagent or a cosmetic.

[0009] Furthermore, the above product has the effect of treating or alleviating skin pigmentation, scaling, erythema, increased wrinkles, skin thickening, reduced elasticity, skin ulceration or edema caused by acute photoaging due to medium-wave ultraviolet radiation.

[0010] Furthermore, the above product is used for external coating.

[0011] The present invention has the following beneficial effects:

[0012] The experimental results show that Gastrodia elata polysaccharide can treat skin damage caused by acute photoaging due to medium-wave ultraviolet radiation, and can effectively improve symptoms such as skin pigmentation, scaling, erythema, increased wrinkles, skin thickening, reduced elasticity, skin ulceration and edema, and has great application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is the result diagram of the effect of Gastrodia elata polysaccharide on the skin appearance of mice with acute photoaging induced by UVB;

[0014] Figure 2 This is the appearance scoring result of Gastrodia elata polysaccharide in treating UVB acute photoaging mouse skin;

[0015] Figure 3 This is a graph showing the effect of Gastrodia elata polysaccharide on the skin tissue of mice induced by acute UVB light damage. DETAILED DESCRIPTION

[0016] The principles and features of the present invention are described below, and the examples are only used to explain the present invention and are not used to limit the scope of the present invention. If no specific conditions are specified in the embodiments, they are carried out according to normal conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments is not specified, they are all conventional products that can be purchased commercially.

[0017] Example 1

[0018] The extraction steps of Gastrodia elata polysaccharide are as follows:

[0019] (1) Fresh tubers of Gastrodia elata were homogenized with water in a mass ratio of 1:8, extracted in a 70°C constant temperature water bath for 70 min, and then centrifuged at 12,000 rpm for 10 min. After filtration, the residue was mixed with half the mass of water, and the extraction and filtration were repeated;

[0020] (2) combining the two filtrates and concentrating them by rotary evaporation at 50°C to one third of the filtrate volume;

[0021] (3) The concentrated filtrate was mixed with Sevag reagent at a volume ratio of 5:1, placed in a shaker at 200 rpm for 1 h, and then centrifuged at 9000 rpm for 10 min. The upper sugar solution was collected and repeated 10 times until there was no white substance between the two phases.

[0022] (4) Add 4 times the volume of anhydrous ethanol to the sugar solution, precipitate at 4°C for 12 h, transfer the precipitated polysaccharide to a centrifuge tube, centrifuge at 5000 rpm for 5 min, discard the supernatant, and then add an appropriate volume of ethanol to wash the precipitate, repeating twice;

[0023] (5) Place the polysaccharide in a 37°C oven to evaporate the ethanol, add an appropriate amount of deionized water, and dissolve the polysaccharide in a 60°C water bath for 2 h. Then remove the insoluble matter by centrifugation, and repeat twice;

[0024] (6) Place the sugar solution into a dialysis bag and dialyze using distilled water. Change the distilled water every 6 hours for a total of 24 hours.

[0025] (7) The dialyzed sugar solution is vacuum freeze-dried to obtain Gastrodia elata polysaccharide.

[0026] Example 2

[0027] Effects of Gastrodia elata polysaccharide on skin appearance of mice with acute photoaging by UVB

[0028] (1) Experimental animals and modeling

[0029] The experiments used SPF-grade C57BL / 6J male mice, 5 weeks old, weighing 18-22 g, provided by Spef (Beijing) Biotechnology Co., Ltd. The animal experimental protocol was reviewed and approved by the Animal Care and Use Committee of Kunming University of Science and Technology.

[0030] The mice were anesthetized with Avertin (tribromoethanol) (20 μL / g); the hair on the back of the mice was shaved with a shaver (2 cm × 2 cm), and the excess fine and short hair was removed with a depilatory cream, the residual depilatory cream was scrubbed with sterile saline, and then disinfected with 75% alcohol; the skin condition of the mice was observed to ensure that the skin of the mice to be modeled had no wounds, damage or scratches, the eyes of the mice were covered with gauze, and the mice were fixed on the cardboard with a tie strip; UV aging box ZW-40 (Shaoxing Zhicheng Instrument Co., Ltd.) was used for modeling, and the UVB (15W, 2 lamps) were preheated for 10 minutes, and then irradiated continuously for 2 days at a distance of 15 cm from the back skin of the mice, 30 minutes a day, and the skin damage of the mice was observed; when the skin of the mice showed symptoms such as rashes, edema and ulcers, the modeling was successful.

[0031] (2) Experimental sample preparation and grouping

[0032] Fresh Gastrodia elata was mixed with water in a ratio of 1:4, homogenized and pasteurized for 30 minutes, and set aside; Gastrodia elata polysaccharide prepared in Example 1 was taken and set aside.

[0033] The experiment was divided into 5 groups: control group (CK), model group (M), Gastrodia elata group (G), Gastrodia elata polysaccharide group (GP), and positive drug group (P), with 10 mice in each group. 200μL of drugs were administered every day for a total of 18 days. The mice in the control group (CK) were not modeled, and 200uL of sterile water was applied every day; the model group (M) was not administered after modeling; after modeling in the positive drug group (P), 0.2g of Carbomer moist gel was evenly applied on the back of the mice; after modeling in the Gastrodia elata group (G), 0.2g of Gastrodia elata was applied; after modeling in the Gastrodia elata polysaccharide group (GP), 0.2g of Gastrodia elata polysaccharide was applied every day. The experiment lasted for 18 days, and the changes in the skin on the back of the mice were observed every day, and photographs were taken and recorded. The results are as follows: Figure 1 shown.

[0034] (3) Mouse skin scoring

[0035] After the irradiation, seven experimenters scored the degree of skin damage in the irradiated area on the back of the mice in accordance with the "Technical Specifications for Safety of Cosmetics". The scoring criteria are shown in Table 1.

[0036] Table 1 Mouse skin appearance scoring criteria

[0037]

[0038] The skin scores of mice in each group are shown in Figure 2 shown.

[0039] Depend on Figure 1 and Figure 2 It can be seen that on the 4th to 6th day, the skin condition of the mice was the most serious, and then each group gradually improved; starting from the 10th day of the experiment, except for the CK group, the GP group had the lowest score, followed by the P group and the G group, and the M group had the highest score; on the 18th day, the skin of the mice in the GP group was completely recovered; it can be seen that Gastrodia elata polysaccharide has a significant therapeutic effect on UVB acute photoaging mouse skin damage.

[0040] Example 3

[0041] Effects of Gastrodia elata polysaccharide on skin tissue of mice with acute photoaging caused by UVB

[0042] The experimental modeling and design were consistent with those in Example 2. On the 18th day, the mice were anesthetized and killed by cervical dislocation. The dorsal skin tissue was taken for cross-sectional H&E staining to observe the structure of each layer of the mouse skin tissue. The results were as follows: Figure 3 shown.

[0043] The results showed that on the 5th day, the epidermis of the skin in each group was incomplete, with disordered structure and unclear boundaries; hyperkeratosis, hyperplasia of epidermal spinous cells, and elongated skin processes; granulation tissue was seen in the deep layer of the skin, fibroblast hyperplasia, new blood vessels were seen in the granulation tissue, and a large number of lymphocytes and granulocytes were infiltrated. On the 18th day, the epidermis of the skin tissue in each group was intact and thin. In the superficial dermis of group M, a very small amount of fibrous connective tissue hyperplasia was seen, and the skin appendages hair follicles and sebaceous glands were scattered, and a small amount of granulocytes and lymphocytes were infiltrated in the dermis and subcutaneous tissue. In the injured part of the skin tissue in group G, the epidermis was intact, and moderate keratosis was seen; the thickness of the epidermis was uneven, and the spinous layer was thickened in the moderate range; there was little elongation of the skin processes; more new granulation tissue hyperplasia and repair, more fibrous connective tissue hyperplasia, accompanied by a very small amount of lymphocyte infiltration, a small amount of new blood vessels, and congestion; slight bleeding was seen; the skin appendages at the injured part were reduced, and there were few follicle keratin plugs. In the P group, very few lymphocytes were found in the dermis and subcutaneous tissue. In the GP group, collagen fibers in the dermis were neatly arranged, and the hair follicles and sebaceous glands of the skin appendages were scattered, with a large number of hair follicles. Very few lymphocytes were found in the dermis and subcutaneous tissue. It can be seen that the skin of mice treated with Gastrodia elata polysaccharide recovered best.

[0044] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent substitution or improvement made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. Application of Gastrodia elata polysaccharide in the preparation of products for the treatment of acute photoaging skin damage caused by ultraviolet B irradiation.

2. The use according to claim 1, characterized in that The gastrodia elata polysaccharide is a gastrodia elata extract prepared by water extraction and alcohol precipitation method.

3. The use according to claim 1, characterized in that The product is a medicine, a reagent or a cosmetic.

4. The use according to claim 1, characterized in that The product has the effect of treating or alleviating skin pigmentation, scaling, erythema, increased wrinkles, skin thickening, reduced elasticity, skin ulceration or edema caused by acute photoaging due to medium-wave ultraviolet radiation.

5. The use according to claim 1, characterized in that The product is used for external application.

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