A kind of oral preparation of radix trepang polysaccharide and preparation method thereof

Abstract

The present invention relates to an oral preparation of radix darbe polysaccharides and a preparation method thereof, aiming to overcome the deficiencies of traditional Chinese medicine preparations in terms of taste, portability and bioavailability. The main component of the preparation is a polysaccharide extract from radix darbe, supplemented with auxiliary materials such as starch and licorice extract, and is prepared by reasonable proportioning and modern preparation technology. The preparation can be in the form of capsules, oral liquids or powders, has the characteristics of good taste and easy to carry, and is suitable for use by different groups of people. The preparation method of the present invention includes the steps of washing, slicing, extracting, concentrating and drying fresh radix darbe, ensuring the retention and stability of the effective ingredients. By improving the accuracy of the dosage and the bioavailability of the effective ingredients, the present invention can significantly enhance the anti-hypoxia and antioxidant effects of radix darbe, provide a safer and more convenient use experience, and provide new ideas and application prospects for the modernization of traditional Chinese medicine.

Description

Technical Field

[0001] The invention relates to the technical field of radix treotii oral preparations, in particular to an radix treotii polysaccharide oral preparation and a preparation method thereof. Background Art

[0002] The root of radish, also known as turnip root, root vegetable, round root, gray radish, etc., is a common vegetable belonging to the subfamily Brassicae of the cruciferous family. At present, the origin of radish is all over the Qinghai-Tibet Plateau in my country. It is widely planted by residents in the plateau areas of my country and used to relieve altitude sickness of humans and livestock. It is one of the commonly used medicinal materials in traditional Chinese medicine. As an important Chinese medicinal material, according to the classic masterpiece "Medical Prescriptions Four Continued" recognized in the field of Tibetan medicine, radish has the effects of sweet taste, warm nature, clearing away heat and detoxification, and nourishing and increasing oxygen. Its main ingredients include a variety of minerals, such as potassium, calcium, magnesium, phosphorus, etc. These minerals play an important role in bone health, nerve conduction, and cell metabolism. It is rich in vitamins, especially vitamin C, which helps anti-oxidation. These active ingredients give radish a unique pharmacological effect, making it widely used in traditional Chinese medicine treatment.

[0003] However, despite the significant medicinal value of radix quatrainii, the main preparation forms on the market are still decoctions, wine preparations and granules. These traditional preparations have some shortcomings during use, such as poor taste, inconvenience in taking, and difficulty in controlling dosage. For many people, especially young people who are averse to traditional Chinese medicine, the acceptance of traditional preparations is low. This situation prompted us to explore new oral preparations of radix quatrainii to meet the needs of modern people for the use of Chinese medicine.

[0004] At present, there are many types of Chinese medicine preparations, but due to the limitations of preparation technology and materials, traditional preparations usually face the following problems:

[0005] Taste problem: Decoctions and granules often have a bitter taste, which is difficult for some people to accept, especially children and the elderly. The bitter taste not only affects the comfort of taking the medicine, but may also lead to a decrease in people's compliance with medication.

[0006] The dosage is difficult to control: The dosage of traditional preparations is usually not precise enough, and there may be a risk of overdose or underdose when taking, which will affect the therapeutic effect.

[0007] Poor portability: Traditional decoctions are not easy to carry when going out, which limits their use in daily life.

[0008] In recent years, with the development of modern medical technology, the pharmacological research on radix gentiana has gradually increased. Studies have shown that radix gentiana has the following pharmacological effects:

[0009] Anti-hypoxia effect: After intervention, radix quatica polysaccharides can significantly increase the number of red blood cells and hemoglobin concentration in the blood of hypoxic mice, and can significantly prolong the survival time of hypoxic mice under various environments, indicating that radix quatica polysaccharides have certain anti-hypoxia effects. Many studies have found that radix quatica extracts have significant antioxidant activity and can scavenge free radicals in the body, thereby reducing the risk of chronic diseases. Radix quatica polysaccharides also have a strong ability to scavenge DPPH free radicals, ABTS free radicals and hydroxyl free radicals, and have significant antioxidant activity in vivo and in vitro.

[0010] In view of the various shortcomings of traditional preparations in practical applications, the research and development of new oral preparations is particularly important. Through modern preparation technologies, such as micronization, spray drying, coating technology, etc., the solubility and bioavailability of genkwa can be effectively improved. In addition, the selection of appropriate excipients, such as starch, licorice, wolfberry, etc., can not only improve the taste, but also improve the stability of the preparation.

[0011] Therefore, we urgently need to design an oral preparation of Radix Perillae polysaccharides and a preparation method thereof to solve the above problems. Summary of the invention

[0012] The purpose of the present invention is to provide an oral preparation of genkwa polysaccharide and a preparation method thereof in view of the deficiencies of the prior art, so as to solve the problems raised in the background technology.

[0013] To achieve the above object, the present invention provides the following technical solution: an oral preparation of genkwa polysaccharide, the preparation comprising the following ingredients:

[0014] A polysaccharide extract of genkwa root, which is extracted from fresh genkwa root and has an active ingredient content of 10-20%;

[0015] The auxiliary materials include liquorice extract, and the weight of the liquorice extract accounts for 5%-10% of the total auxiliary materials.

[0016] As a preferred technical solution of the present invention, the preparation is in the form of capsule, oral liquid or powder, and the shell of the capsule is gelatin or plant cellulose material.

[0017] The present invention also provides a method for preparing an oral preparation of Radix Perillae polysaccharides, comprising the following steps:

[0018] a) Prepare fresh coriander roots, wash and slice them into slices with a thickness of 2-5 mm, and dry them naturally in a well-ventilated and sunny place;

[0019] i) at room temperature, the dried genus elm slices were crushed with an ultrafine grinder, and then petroleum ether was added and stirred for 30 minutes;

[0020] ii) filtering the mixture obtained in the previous step under negative pressure to obtain an undissolved residue, and leaving it to stand overnight in a fume hood (to evaporate and remove the remaining petroleum ether);

[0021] iii) Dry the treated radix dasyphyllae in an oven at 50 degrees Celsius until the weight does not change, grind and pulverize the radix dasyphyllae through a 40-mesh sieve, and then carry out the next step of polysaccharide extraction.

[0022] b) using ultrasound-assisted hot water extraction to extract polysaccharides, wherein pure water is used as a polysaccharide extractant, and the specific steps are as follows:

[0023] i) mixing the treated genus arborvitae with water at a liquid-to-solid ratio of 1:49 (g / mL);

[0024] ii) setting extraction conditions, including extraction temperature of 48° C. and extraction time of 65 minutes;

[0025] iii) extracting under the action of ultrasound to fully release the effective components of the polysaccharide;

[0026] c) filtering the extract to remove the residue to obtain a clear extract, and then concentrating the extract to form a concentrated extract to 10-20% of the original volume.

[0027] As a preferred technical solution of the present invention, the concentration step adopts reduced pressure concentration or normal pressure concentration, and the temperature is controlled at 50-70°C.

[0028] As a preferred technical solution of the present invention, the steps are as follows:

[0029] d) vacuum freeze-drying the concentrated extract to obtain a dry powder of radix quatrain polysaccharides, and spray drying the powder under the following conditions:

[0030] As a preferred technical solution of the present invention, the steps are as follows:

[0031] e) mixing the genus quatrain polysaccharide dry powder with auxiliary materials in proportion to form a uniform mixture, wherein the uniformity of the mixture should reach more than 95%.

[0032] As a preferred technical solution of the present invention, the composition ratio of the mixture is:

[0033] The mass ratio of genkwa polysaccharide dry powder: starch: licorice extract is 2:0.5:1.5.

[0034] As a preferred technical solution of the present invention, the steps are as follows:

[0035] f) filling the mixture into capsules with a filling amount of 200-500 mg. After the capsules are filled, they are automatically sealed and the integrity rate of the capsules is ≥98%.

[0036] As a preferred technical solution of the present invention, g) diluting the concentrated extract to a concentration suitable for taking, adding appropriate amounts of stabilizers and sweeteners to mix until uniform, and finally preparing an oral liquid; the oral liquid has an active ingredient content of 1-5%, and the prepared oral liquid is packaged and stored in sterile glass bottles or aluminum-plastic packaging.

[0037] As a preferred technical solution of the present invention, the preparation is packaged in the final step, using vacuum packaging or aluminum foil packaging, and the storage conditions are dry and cool place, and the temperature does not exceed 25°C.

[0038] Compared with the prior art, the present invention has the following beneficial effects:

[0039] The present invention adopts modern preparation technology to ensure that the active ingredients of each capsule or powder are strictly proportioned to achieve precise dosage control. In addition, by optimizing the extraction process and preparation form, the bioavailability of the active ingredients has been significantly improved, making the anti-hypoxia effect more significant.

[0040] Solid preparations such as capsules and oral liquids or powders are easier to carry than traditional decoctions and are convenient for patients to use in daily life. At the same time, the use of vacuum or aluminum foil packaging design ensures the stability and safety of the preparations, reduces the risk of degradation of active ingredients during storage and transportation, and provides a more convenient medication option for people entering the plateau. DETAILED DESCRIPTION

[0041] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0042] The specific implementation of the present invention is described in detail below in conjunction with multiple embodiments.

[0043] Example 1: Preparation of Radix Georgi polysaccharide capsule preparation

[0044] Material:

[0045] Fresh cilantro: 5000 g

[0046] Dried cilantro: 500g

[0047] Pure water: 24500 ml (liquid to material ratio 1:49)

[0048] Starch: 200 g

[0049] Licorice extract: 50 g

[0050] Steps: washing, slicing and drying: Wash the fresh radix garlandii thoroughly with clean water, remove dirt and impurities, cut into slices about 3 mm thick, and dry them naturally in a well-ventilated and sunny place; crush the radix garlandii slices with an ultrafine grinder, then add petroleum ether and stir for 30 minutes; filter the mixture after the previous step under negative pressure to obtain undissolved residues, and leave them in a fume hood overnight (to evaporate and remove the remaining petroleum ether); dry the treated radix garlandii in an oven at 50 degrees Celsius until the weight no longer changes. Grind and pulverize further to pass through a 40-mesh sieve for later use, and perform ultrasonic-assisted extraction to fully release the effective components of the polysaccharide.

[0051] Ultrasonic-assisted hot water extraction:

[0052] a) Mix the processed radix guajavaeformis with pure water, making sure the liquid-to-solid ratio is 1:49.

[0053] b) setting the extraction conditions in the ultrasonic processor to 48°C and 65 minutes,

[0054] c) After the extraction is completed, the extract is filtered using filter paper to remove solid residues to obtain a clear extract.

[0055] Concentration: The obtained extract was placed in a vacuum concentrator, the temperature was set at 50°C, and concentrated to 10% of the original volume, and finally about 500 ml of concentrated solution was obtained.

[0056] Spray drying: The concentrated liquid was processed by a spray dryer. The spray drying conditions were as follows: the inlet temperature was set to 85 degrees Celsius, the injection volume was 390 ml / h, the ventilation volume was 0.68 M, the compressed air pressure was 12x10 Kpa, the spray dryer was turned on, and the injection was started when the sample inlet temperature was 85 degrees Celsius. Finally, about 40-50 grams of genkwa polysaccharide dry powder was obtained, and the moisture content of the dry powder was ≤5%.

[0057] Mixing and formulation: Mix the dried powder of genkwa polysaccharide with starch and licorice extract in proportion (see Table 1), mix them thoroughly in a high-speed mixer to ensure uniformity, and then fill the capsules.

[0058] Packaging: Vacuum pack the filled capsules and store them in a dry and cool place away from direct sunlight.

[0059] Table 1: Proportions of mixture ingredients

[0060]

[0061] Example 2: Preparation of Oral Liquid Preparation of Radix Perillae Polysaccharides

[0062] Material:

[0063] Fresh cilantro: 5000 g

[0064] Dried cilantro: 500 g

[0065] Pure water: 24500 ml (liquid to material ratio 1:49)

[0066] Licorice extract: 50 g

[0067] Sweeteners (such as xylitol or erythritol): appropriate amount

[0068] Stabilizer (such as sodium citrate): appropriate amount

[0069] step:

[0070] Wash, slice and dry:

[0071] Wash the fresh coriander roots thoroughly with clean water, remove dirt and impurities, cut into slices about 3 mm thick, and dry them naturally in a well-ventilated and sunny place.

[0072] Extraction of polysaccharides:

[0073] a) crushing the dried genus lycopodii slices with an ultrafine grinder and passing through a 40-mesh sieve for later use;

[0074] b) mixing the genus quatrain powder with pure water at a liquid-to-solid ratio of 1:49 (g / mL);

[0075] c) performing hot water extraction in an ultrasonic processor at a temperature of 48° C. and an extraction time of 65 minutes;

[0076] d) The extract is filtered to remove solid residues to obtain a clear extract.

[0077] concentrate:

[0078] The extract is concentrated to 10%-20% of the original volume under reduced pressure, and the concentration temperature is controlled within 50°C.

[0079] Preparation of oral solution:

[0080] a) diluting the concentrate to a concentration suitable for administration (active ingredient content of 1%-5%);

[0081] b) adding appropriate amount of liquorice extract, sweetener and stabilizer, stirring well to prepare oral solution;

[0082] c) Test the uniformity and stability of oral liquid to ensure that the quality meets the requirements.

[0083] Repackaging and packaging: The prepared oral liquid is repacked into sterile glass bottles under aseptic conditions and sealed with aluminum plastic. The finished product should be stored in a cool and dry place at a temperature not exceeding 25°C.

[0084]

[0085] Example 3: Preparation of Polysaccharide Powder

[0086] Ingredients: 3000g fresh coriander root;

[0087] Dried cilantro: 300 g;

[0088] Pure water: 14700 ml (liquid to material ratio 1:49)

[0089] Starch: 150 g

[0090] Licorice extract: 30 g

[0091] Steps: washing, slicing and drying: Wash the fresh radix garlandii thoroughly with clean water, remove dirt and impurities, cut into slices about 3 mm thick, and dry them naturally in a well-ventilated and sunny place; crush the radix garlandii slices with an ultrafine grinder, then add petroleum ether and stir for 30 minutes; filter the mixture after the previous step under negative pressure to obtain undissolved residues, and leave them in a fume hood overnight (to evaporate and remove the remaining petroleum ether); dry the treated radix garlandii in an oven at 50 degrees Celsius until the weight no longer changes. Grind and pulverize further to pass through a 40-mesh sieve for later use, and perform ultrasonic-assisted extraction to fully release the effective components of the polysaccharide.

[0092] Ultrasonic-assisted hot water extraction:

[0093] a) Place the processed radix guajavaeformis into pure water, keeping the liquid-to-solid ratio at 1:49.

[0094] b) Set the extraction conditions in an ultrasonic processor to 48°C and 65 minutes.

[0095] c) After extraction, filter to obtain the extract.

[0096] Concentration: Concentrate the extract to 400 ml and control the temperature below 50°C.

[0097] Spray drying: Spray drying obtains about 40-50 g of dry powder with a moisture content of ≤5%.

[0098] Mixing and Formulation: Mix the dry powder with starch and licorice extract in proportion (see Table 3), then make into powder and sieve with a fine sieve to ensure uniformity.

[0099] Packaging: vacuum pack the powder and store it in a cool place to ensure moisture-proof and stability.

[0100] Table 3: Mixture composition ratio

[0101]

[0102] The above examples show in detail the preparation methods of different forms of oral preparations of Radix Euphorbiae, and provide a wealth of materials, steps and data tables to ensure the repeatability and effectiveness of each step. The preparation process of each example includes specific data of the extraction, concentration, drying and molding process of the active ingredients to support the practicality and scientificity of the present invention.

[0103] The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in the field. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An oral preparation of genkwa polysaccharide, characterized in that: The preparation comprises the following ingredients: A polysaccharide extract of genkwa root, which is extracted from fresh genkwa root and has an active ingredient content of 10-20%; The auxiliary materials include starch and liquorice extract, wherein the weight of the liquorice extract accounts for 5%-10% of the total auxiliary materials.

2. The oral preparation of Radix Gentianae polysaccharides according to claim 1, characterized in that: The preparation is in the form of capsule, oral liquid or powder, and the shell of the capsule is made of gelatin or plant cellulose material.

3. A method for preparing an oral preparation of genkwa polysaccharide, characterized in that: The following steps are involved: a) Prepare fresh coriander roots, wash and slice them into 2-5 mm thick slices, and allow them to dry naturally in a well-ventilated, sunny place. i) at room temperature, the dried genus elm slices were crushed with an ultrafine grinder, and then petroleum ether was added and stirred for 30 minutes; ii) filtering the mixture obtained in the previous step under negative pressure to obtain an undissolved residue, and leaving it to stand in a fume hood overnight to evaporate and remove the remaining petroleum ether; iii) Dry the treated radix dasyphyllae in an oven at 50 degrees Celsius until the weight does not change, grind and pulverize the radix dasyphyllae through a 40-mesh sieve, and then carry out the next step of polysaccharide extraction. b) using ultrasound-assisted hot water extraction to extract polysaccharides, wherein water is used as a polysaccharide extractant, and the specific steps are as follows: i) mixing the treated genus arborvitae with water at a liquid-to-solid ratio of 1:49 (g / mL); ii) setting extraction conditions, including extraction temperature of 48°C, extraction time of 65 minutes, and sample mesh size of 40 mesh; iii) extracting under the action of ultrasound to fully release the effective components of the polysaccharide; c) filtering the extract to remove the residue to obtain a clear extract, and then concentrating the extract to form a concentrated extract to 10-20% of the original volume.

4. The method for preparing an oral preparation of Radix Gentianae polysaccharides according to claim 3, characterized in that: The concentration step adopts reduced pressure concentration or normal pressure concentration, and the temperature is controlled at 50-70°C.

5. The method for preparing an oral preparation of Radix Gentianae polysaccharides according to claim 3, characterized in that: Includes steps: d) spray drying the concentrated extract to obtain dry powder of Polysaccharide from Radix Euphorbiae Radix.

6. The method for preparing an oral preparation of Radix Gentianae polysaccharides according to claim 3, characterized in that: Includes steps: e) mixing the genus quatrain polysaccharide dry powder with auxiliary materials in proportion to form a uniform mixture, wherein the uniformity of the mixture should reach more than 95%.

7. The method for preparing an oral preparation of Radix Gentianae polysaccharides according to claim 6, characterized in that: The composition ratio of the mixture is: The mass ratio of genkwa polysaccharide dry powder: starch: licorice extract is 2:0.5:1.

5.

8. The method for preparing an oral preparation of Radix Gentianae polysaccharides according to claim 3, characterized in that: Includes steps: f) filling the mixture into capsules, wherein the capsule filling amount is 200-500 mg; After the capsule is filled, it is automatically sealed, and the integrity rate of the capsule is ≥98%.

9. The method for preparing an oral preparation of Radix Gentianae polysaccharides according to claim 3, characterized in that: Includes steps: g) diluting the concentrated extract to a concentration suitable for administration, adding an appropriate amount of stabilizer and sweetener to mix evenly, and finally preparing an oral liquid; the oral liquid has an active ingredient content of 1-5%, and the prepared oral liquid is packaged and stored in sterile glass bottles or aluminum-plastic packaging.

10. The method for preparing an oral preparation of Radix Gentianae Polysaccharides according to claim 3, characterized in that: The preparation is packaged in the final step, using vacuum packaging or aluminum foil packaging, and the storage condition is a dry and cool place with a temperature not exceeding 25°C.

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