A kind of Cistanche deserticola and Polygonatum sibiricum compound concentrated liquid and preparation method thereof
By using raw materials such as Cistanche and Polygonatum in the Chinese medicine compound concentrate, combined with supercritical CO2 extraction and low temperature enzymatic decomposition technology, the existing Chinese medicine compound concentrate has solved the problem of single effect and low extraction efficiency, and achieved the effect of multi-efficacy comprehensive health care and efficient extraction.
Patent Information
- Application Number
- CN202411878514.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-19
- Publication Date
- 2025-04-04
AI Technical Summary
The existing traditional Chinese medicine compound concentrate has a single effect and cannot meet the comprehensive requirements of consumers' various health care needs. In addition, the traditional extraction methods are inefficient and have many impurities, so complex ingredients cannot be fully extracted.
Cistanche deserticola and Polygonatum are used as the main raw materials, combined with mulberry, Ganoderma lucidum spore powder and other ingredients, and through supercritical CO2 extraction and low-temperature enzymatic decomposition technology, combined with nanofiltration membrane, electrodialysis and high-voltage homogenization processes, a compound concentrate is prepared, and the multifunctionality of the product is enhanced by adding auxiliary materials such as aloe vera polysaccharide and grape seed extract.
It realizes multi-functional comprehensive health care, significantly improves the extraction rate and purity of the product, ensures the full extraction and stability of all active ingredients, extends the shelf life of the product, and improves the safety and bioavailability of the product.
Smart Images
Figure CN119745995A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a Cistanche deserticola and Polygonatum sibiricum compound concentrated liquid and a preparation method thereof. Background Art
[0002] With the accelerated pace of modern life and the improvement of people's health awareness, Chinese herbal compound concentrates are favored by more and more consumers as a convenient health care product. Chinese herbal compound concentrates are usually extracted and concentrated from a variety of Chinese herbal raw materials with specific functions. Their advantage is that they can retain a variety of effective ingredients and are easy to carry and take.
[0003] However, the existing Chinese herbal compound concentrates still have some deficiencies in raw material processing, extraction methods, concentration technology and auxiliary material selection. The existing Chinese herbal compound concentrates are mostly designed around a single function, such as only tonifying the kidney, only tonifying qi and blood, etc., which cannot meet the comprehensive requirements of consumers for multiple health care needs. The functions are relatively single, and the existing extraction methods mostly use water extraction or alcohol extraction. These methods take a long time to extract, the extraction rate of effective ingredients is not high, and impurities are easily introduced. Traditional extraction methods have poor extraction effects on complex components and cannot ensure that all effective ingredients can be fully extracted. Summary of the invention
[0004] In view of the deficiencies in the prior art, the present invention provides a Cistanche and Polygonatum compound concentrated solution and a preparation method thereof, which solves the problem that the concentrated solution of traditional Chinese medicine has a single effect.
[0005] To achieve the above objectives, the present invention is implemented through the following technical scheme: a Cistanche and Polygonatum compound concentrated solution, comprising the following percentages of raw materials: 35% to 40% Cistanche, 35% to 40% Polygonatum, 10% to 15% mulberry, 8% to 10% Ganoderma lucidum spore powder, and 7% to 8% auxiliary materials.
[0006] Preferably, the auxiliary materials include potassium sorbate, and one or more of aloe polysaccharides, grape seed extract, xylitol, small molecule peptides, vitamin C, licorice extract, oligofructose, sodium hyaluronate, oat β-glucan, erythritol, tea polyphenols, L-carnitine, citric acid, probiotic powder, trehalose, and galacto-oligosaccharides.
[0007] A method for preparing a Cistanche deserticola and Polygonatum sibiricum compound concentrated solution comprises the following steps: S1. Raw material pretreatment: use an ultrasonic cleaner to deeply clean Cistanche deserticola, Polygonatum sibiricum, and mulberry to remove microorganisms and residues on the surface, and dry the cleaned raw materials in a low-temperature dryer to avoid damage to the active ingredients; S2 extraction; the dried Cistanche deserticola, Polygonatum and mulberry were extracted in a supercritical CO2 extraction device, and the extract was collected for later use; S3. Low temperature enzymatic hydrolysis; adding the collected extract to the hydrolase, adding the extract to the enzymatic reactor, stirring evenly after adding the enzyme, inactivating the enzyme, filtering, and retaining the enzymatic solution; S4. Filtration and purification: Use nanofiltration membrane to filter the enzymatic hydrolysate to remove fine impurities and microorganisms to ensure the purity of the extract. Use electrodialysis technology to further remove harmful ions and heavy metals in the extract to improve product safety. S5. Concentration; the purified extract is concentrated by a low-temperature membrane concentration device; S6. Adding auxiliary materials: Add the dissolved auxiliary materials to the concentrated extract and use a high-pressure homogenizer to homogenize to ensure that all auxiliary materials are fully mixed. Preferably, in step S1, the ultrasonic cleaning machine is set to have a frequency of 28 kHz-30 kHz, a power of 500 W-550 W, and a time of 10-15 minutes, and the low-temperature dryer is set to have a temperature of 40° C.-45° C. and a time of 12 h to 18 h.
[0008] Preferably, the extraction equipment in step S2 is set to have a pressure of 30 MPa-40 MPa, a temperature of 40° C.-45° C., and an extraction time of 1-1.5 hours.
[0009] Preferably, the hydrolase in step S3 includes 0.5% to 0.7% cellulase and 0.5% to 0.7% protease, the temperature of the enzymatic hydrolysis reactor is controlled at 40°C-50°C, the pH is controlled between 6.0-6.5, and after 1-1.5 hours of enzymatic hydrolysis, the temperature is heated to 80°C-85°C.
[0010] Preferably, in step S4, the pore size of the nanofiltration membrane is 0.2-0.3 μm, the pressure difference is 0.4-0.5 MPa, the current density is controlled at 100-120 A / m², and the time is 30-45 minutes.
[0011] Preferably, in step S5, the low-temperature membrane concentration equipment is set at a temperature of 45°C-50°C, and the concentration is between one-fifth and one-sixth.
[0012] Preferably, in step S6, the pressure of the high-pressure homogenizer is 150-170 MPa, the temperature is 40°C-45°C, the time is 3-6 minutes, and the constant temperature stirring tank is used for constant stirring at 40°C-50°C for 10-15 minutes, and the stirring speed is 60-80 rpm.
[0013] Preferably, the method further includes S7. Sterilization and filling: sterilizing the concentrated liquid with pulsed strong light sterilization technology, filling it in a sterile environment, and using an automatic filling machine to fill it into sterile glass bottles.
[0014] The present invention provides a Cistanche deserticola and Polygonatum sibiricum compound concentrated solution and a preparation method thereof. It has the following beneficial effects: 1. By changing the formula, the present invention not only has significant kidney-tonifying and yang-strengthening effects, but also can enhance immunity, improve blood circulation, increase body resistance, relieve fatigue, improve vision and skin elasticity and moisture retention, and achieve multi-functional comprehensive health care. By changing the ratio, it can adapt to people with different health conditions.
[0015] 2. The present invention adopts supercritical CO2 extraction technology, which can efficiently extract multiple effective ingredients in traditional Chinese medicine in a short time, with high extraction rate and few impurities. Through low-temperature enzymatic hydrolysis technology, the purity of the extract and the content of active ingredients are further improved, ensuring that the efficacy of the product is more significant and lasting.
[0016] 3. The present invention uses nanofiltration membrane and electrodialysis technology to completely remove fine impurities and harmful ions, and combines pulsed strong light sterilization technology to ensure the sterility and safety of the product, thereby extending the shelf life of the product. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 It is a flow chart of the present invention. DETAILED DESCRIPTION
[0018] The following will be combined with the drawings in the specification of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0019] Embodiment 1: Please refer to the attached Figure 1The embodiment of the present invention provides a Cistanche and Polygonatum compound concentrated solution, comprising the following percentage raw materials: 35% Cistanche, 35% Polygonatum, 15% mulberry, 8% Ganoderma lucidum spore powder, and 7% auxiliary materials, wherein the auxiliary materials include 2% aloe polysaccharide, 2% grape seed extract, 2% xylitol, 0.5% potassium sorbate, and 0.5% small molecule peptide. Cistanche has significant kidney-tonifying and yang-strengthening effects, and is often used to treat symptoms such as impotence and premature ejaculation caused by kidney deficiency. It can also enhance physical strength, improve fatigue, and improve overall physical fitness. It also contains a variety of antioxidant ingredients, which are helpful in delaying aging. Polygonatum has the effects of invigorating qi and nourishing yin, which can enhance the body's resistance and improve immunity, and has a good alleviating effect on dry cough and chronic cough caused by lung deficiency. Mulberry can nourish yin and blood, improve complexion, and relieve anemia symptoms, and contains rich Rich in vitamin A and antioxidants, it helps to improve vision; Ganoderma lucidum spore powder is the essence of Ganoderma lucidum, rich in polysaccharides and triterpenoids, which can significantly enhance the body's immunity and have anti-inflammatory effects, which can reduce inflammatory responses, help protect the liver and improve liver function; aloe polysaccharides help promote wound healing of skin and tissues, and grape seed extract is rich in proanthocyanidins and has strong antioxidant capacity, which can protect cells from damage by free radicals, help enhance vascular elasticity and improve blood circulation; potassium sorbate can effectively inhibit the growth of bacteria and mold and extend the shelf life of the product; small molecule peptides have a smaller molecular weight and can be more easily absorbed by the human body, thereby improving the bioavailability of the product and helping to enhance the overall efficacy of the product, especially in terms of tonifying the kidney and strengthening the body.
[0020] A method for preparing a Cistanche deserticola and Polygonatum sibiricum compound concentrated solution comprises the following steps: S1. Raw material pretreatment: Use an ultrasonic cleaner to deeply clean Cistanche deserticola, Polygonatum sibiricum, and mulberry to remove surface microorganisms and residues. Dry the cleaned raw materials in a low-temperature dryer to avoid damage to the effective ingredients. The ultrasonic cleaner is set to a frequency of 28 kHz, a power of 500 W, and a time of 10 minutes. The low-temperature dryer is set to a temperature of 40°C and a time of 12 to 18 hours.
[0021] S2. Extraction; the dried Cistanche deserticola, Polygonatum sibiricum and mulberry were placed in a supercritical CO2 extraction device, the pressure was set to 30 MPa, the temperature was set to 40°C, the extraction time was 1 hour, and the extract was collected for later use.
[0022] S3. Low-temperature enzymatic hydrolysis; add 0.5% cellulase and 0.5% protease to the collected extract, add the extract to the enzymatic hydrolysis reactor, stir evenly after adding the enzyme, control the temperature at 40°C and pH 6.0, after 1 hour of enzymatic hydrolysis, heat to 80°C to inactivate the enzyme, filter, and retain the enzymatic hydrolyzate.
[0023] S4. Filtration and purification: The enzymatic hydrolysate is filtered using a nanofiltration membrane with a pore size of 0.2 μm and a pressure difference of 0.4 mPa to remove fine impurities and microorganisms to ensure the purity of the extract. The harmful ions and heavy metals in the extract are further removed through electrodialysis technology to improve the safety of the product. The current density is controlled at 100 A / m² for 30 minutes.
[0024] S5. Concentration: The purified extract is concentrated by low-temperature membrane concentration equipment, the temperature is set to 45°C, and concentrated to about one-fifth. Membrane concentration technology can be carried out at a lower temperature to maintain the activity of the effective ingredients while reducing energy consumption. The temperature is 45°C, and the purified extract is concentrated by membrane concentration equipment to the required volume.
[0025] S6. Addition of auxiliary materials; first dissolve aloe polysaccharide, grape seed extract, xylitol, potassium sorbate, and small molecule peptides in a small amount of water respectively to ensure complete dissolution, add all dissolved auxiliary materials into the concentrated extract, use a high-pressure homogenizer to homogenize to ensure that all auxiliary materials are evenly dispersed, the pressure is 150mPa, the temperature is 40℃, and the time is 3 minutes. Stir at a constant temperature of 40-50℃ for 10 minutes in a constant temperature stirring tank, and the stirring speed is 60 rpm to ensure that all ingredients are completely dissolved.
[0026] S7. Sterilization and filling: Use pulsed light sterilization technology to sterilize the concentrate. Pulsed light can kill bacteria efficiently in a short time to ensure the sterility of the product. Filling is carried out in a sterile environment, using an automated filling machine to fill into sterile glass bottles. Embodiment 2: The embodiment of the present invention provides a Cistanche and Polygonatum compound concentrated liquid, comprising the following raw materials in percentage: 35% of Cistanche, 35% of Polygonatum, 15% of mulberry, 8% of Ganoderma lucidum spore powder, and 7% of auxiliary materials, wherein the auxiliary materials include 2.5% of vitamin C, 2.0% of licorice extract, 2.0% of oligofructose, 0.5% of potassium sorbate, and 0.5% of small molecule peptides. The preparation method remains unchanged. Vitamin C has a strong antioxidant effect, can enhance the immune system, prevent colds and promote wound healing; the licorice extract has anti-inflammatory and antiviral effects, can relieve respiratory inflammation, and improve the overall immunity of the product; oligofructose is a prebiotic, can promote the growth of beneficial intestinal bacteria, and improve digestion and intestinal health.
[0027] Embodiment three: The embodiment of the present invention provides a Cistanche and Polygonatum compound concentrated solution, comprising the following raw materials in percentage: 35% of Cistanche, 35% of Polygonatum, 15% of mulberry, 8% of Ganoderma lucidum spore powder, and 7% of auxiliary materials, wherein the auxiliary materials include 3.0% of oat β-glucan, 2.0% of sodium hyaluronate, 1.5% of erythritol, 0.5% of potassium sorbate, and 0.5% of small molecule peptide. The preparation method remains unchanged. The oat β-glucan has the effects of enhancing the immune system and lowering cholesterol, and can improve the body's resistance; sodium hyaluronate has the effects of moisturizing and anti-wrinkle, and can improve the elasticity and moisture retention of the skin; erythritol is a natural sugar alcohol, has the characteristics of low calories and no tooth decay, and can provide sweetness while reducing calorie intake.
[0028] Embodiment 4: The embodiment of the present invention provides a Cistanche and Polygonatum compound concentrated solution, comprising the following percentages of raw materials: 35% Cistanche, 35% Polygonatum, 15% mulberry, 8% Ganoderma lucidum spore powder, and 7% auxiliary materials, wherein the auxiliary materials include 2.0% L-carnitine, 2.0% tea polyphenols, 1.5% citric acid, 0.5% potassium sorbate, and 1.0% small molecule peptides, and the preparation method remains unchanged. L-carnitine helps fat metabolism, can improve the efficiency of energy utilization in the body, and improve physical fitness; tea polyphenols have significant antioxidant and anti-inflammatory effects, can protect cells from damage by free radicals, and improve overall immune function; citric acid can adjust the acidity of the product, improve the taste, and also has a certain antioxidant effect Embodiment five: The embodiment of the present invention provides a Cistanche deserticola and Polygonatum sibiricum compound concentrated solution, comprising the following percentage raw materials: 35% Cistanche deserticola, 35% Polygonatum sibiricum, 15% mulberry, 8% Ganoderma lucidum spore powder, and 7% auxiliary materials, wherein the auxiliary materials include 2.5% probiotic powder, 2.0% trehalose, 1.5% galacto-oligosaccharide, 0.5% potassium sorbate, and 0.5% small molecule peptide, and the preparation method remains unchanged. Probiotic powder can improve intestinal health, enhance the body's immunity, and prevent digestive tract diseases; trehalose has the function of protecting cells and proteins, and can improve the stability of the product; galacto-oligosaccharide is a prebiotic that can promote the growth of beneficial intestinal bacteria and improve digestion and intestinal health.
[0029] Experimental data: Purpose To evaluate the effects of different excipients of Cistanche and Polygonatum sibiricum compound concentrate on immune function, blood sugar level, blood lipid level and intestinal health.
[0030] Subjects 100 middle-aged and elderly people were divided into a control group and five experimental groups (Example 2 to Example 5), with 20 people in each group.
[0031] Experimental cycle 8 weeks Experimental methods The subjects took it once in the morning and evening every day, 20 ml each time, while the control group did not take the Cistanche and Polygonatum compound concentrated solution.
[0032] Finally, after 8 weeks, the following data were obtained: Group Immunity index improvement (%) Fasting blood sugar reduction (mmol / L) Total cholesterol reduction (mmol / L) Triglyceride reduction (mmol / L) Increase in beneficial intestinal bacteria (CFU / g) Control group 10.2 0.4 0.8 0.5 1.5×10^8 Embodiment 2 18.5 0.7 1.2 0.8 3.2×10^8 Embodiment 3 15.8 0.6 1.3 0.7 3.5×10^8 Embodiment 4 17.1 0.8 1.1 0.9 3.0×10^8 Embodiment 5 16.3 0.7 1.2 0.8 3.8×10^8 In summary, the immune index of all experimental groups was significantly higher than that of the control group, among which the improvement effects of Example 2 and Example 4 were the most obvious, which were 18.5% and 17.1%, respectively. The fasting blood glucose levels of all experimental groups were reduced, and the reduction effect of Example 4 was the most significant, with fasting blood glucose reduced by 0.8 mmol / L. The total cholesterol levels of all experimental groups were reduced, and the reduction effect of Example 3 was the most significant, with total cholesterol reduced by 1.3 mmol / L. The triglyceride levels of all experimental groups were reduced, and the reduction effect of Example 4 was the most significant, with triglycerides reduced by 0.9 mmol / L. The number of beneficial intestinal bacteria in all experimental groups increased, and the increase effect of Example 5 was the most significant, with the number of beneficial intestinal bacteria increased by 3.8×10^8 CFU / g.
[0033] It can be seen that the efficacy of the Cistanche and Polygonatum compound concentrated solution has been significantly improved. Specifically, in terms of immune function enhancement, Example 2 and Example 4 have the best effects, in terms of blood sugar management, Example 4 has the best effect, in terms of blood lipid management, Example 3 has the best effect, in terms of intestinal health, Example 5 has the best effect, and in terms of skin moisturizing, Example 3 has the best effect. It can be adapted to different groups of people by replacing different auxiliary materials.
[0034] In summary, the Cistanche deserticola and Polygonatum sibiricum compound concentrated solution provided by the present invention significantly improves the immune function, blood sugar level, blood lipid level, intestinal health and skin condition of middle-aged and elderly people by adding different auxiliary material formulas.
[0035] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A Cistanche deserticola and Polygonatum sibiricum compound concentrated solution, characterized in that: The raw materials include the following percentages: 35% to 40% of Cistanche deserticola, 35% to 40% of Polygonatum sibiricum, 10% to 15% of mulberry, 8% to 10% of Ganoderma lucidum spore powder and 7% to 8% of auxiliary materials.
2. The Cistanche deserticola and Polygonatum sibiricum compound concentrated solution according to claim 1, characterized in that: The auxiliary materials include potassium sorbate, and one or more of aloe polysaccharides, grape seed extract, xylitol, small molecule peptides, vitamin C, licorice extract, oligofructose, sodium hyaluronate, oat beta-glucan, erythritol, tea polyphenols, L-carnitine, citric acid, probiotic powder, trehalose, and galacto-oligosaccharides.
3. The method for preparing a Cistanche deserticola and Polygonatum sibiricum compound concentrated solution according to claim 1 or 2, characterized in that: The following steps are involved: S1. Raw material pretreatment: use an ultrasonic cleaner to deeply clean Cistanche deserticola, Polygonatum sibiricum, and mulberry to remove microorganisms and residues on the surface, and dry the cleaned raw materials in a low-temperature dryer to avoid damage to the active ingredients; S2 extraction; the dried Cistanche deserticola, Polygonatum and mulberry were extracted in a supercritical CO2 extraction device, and the extract was collected for later use; S3. Low temperature enzymatic hydrolysis; adding the collected extract to the hydrolase, adding the extract to the enzymatic reactor, stirring evenly after adding the enzyme, inactivating the enzyme, filtering, and retaining the enzymatic solution; S4. Filtration and purification: Use nanofiltration membrane to filter the enzymatic hydrolysate to remove fine impurities and microorganisms to ensure the purity of the extract. Use electrodialysis technology to further remove harmful ions and heavy metals in the extract to improve product safety. S5. Concentration; the purified extract is concentrated by a low-temperature membrane concentration device; S6. Addition of auxiliary materials: Add dissolved auxiliary materials to the concentrated extract and use a high-pressure homogenizer to homogenize to ensure that all auxiliary materials are fully mixed.
4. The method for preparing a Cistanche deserticola and Polygonatum sibiricum compound concentrated solution according to claim 3, characterized in that: In step S1, the ultrasonic cleaning machine is set to have a frequency of 28 kHz-30 kHz, a power of 500 W-550 W, and a time of 10-15 minutes, and the low-temperature drying machine is set to have a temperature of 40° C.-45° C. and a time of 12 hours to 18 hours.
5. The method for preparing a Cistanche deserticola and Polygonatum sibiricum compound concentrated solution according to claim 3, characterized in that: In step S2, the extraction equipment is set to have a pressure of 30MPa-40MPa, a temperature of 40°C-45°C, and an extraction time of 1-1.5 hours.
6. The method for preparing a Cistanche deserticola and Polygonatum sibiricum compound concentrated solution according to claim 3, characterized in that: In step S3, the hydrolase comprises 0.5% to 0.7% cellulase and 0.5% to 0.7% protease. The temperature of the enzymatic reactor is controlled at 40° C. to 50° C., and the pH is controlled between 6.0 and 6.
5. After 1 to 1.5 hours of enzymatic hydrolysis, the reactor is heated to 80° C. to 85° C.
7. The method for preparing a Cistanche deserticola and Polygonatum sibiricum compound concentrated solution according to claim 3, characterized in that: In step S4, the pore size of the nanofiltration membrane is 0.2-0.3 μm, the pressure difference is 0.4-0.5 MPa, the current density is controlled at 100-120 A / m², and the time is 30-45 minutes.
8. The method for preparing a Cistanche deserticola and Polygonatum sibiricum compound concentrated solution according to claim 3, characterized in that: In step S5, the temperature of the low-temperature membrane concentration equipment is set at 45°C-50°C, and the concentration is between one-fifth and one-sixth.
9. The method for preparing a Cistanche deserticola and Polygonatum sibiricum compound concentrated solution according to claim 3, characterized in that: In step S6, the pressure of the high-pressure homogenizer is 150-170 MPa, the temperature is 40°C-45°C, the time is 3-6 minutes, and the constant temperature stirring tank is used to stir at 40°C-50°C for 10-15 minutes, and the stirring speed is 60-80 rpm.
10. The method for preparing a Cistanche deserticola and Polygonatum sibiricum compound concentrated solution according to claim 3, characterized in that: Also includes: S7. Sterilization and filling: Sterilize the concentrate using pulsed light sterilization technology, fill it in a sterile environment, and use an automated filling machine to fill it into sterile glass bottles.