Gold@platinum nanozyme-mediated CRISPR-Cas12a sensing system and its application in biological detection
Through the CRISPR-Cas12a detection visual biosensing system mediated by gold@platinum nanoenzyme, combined with RPA and CRISPR/Cas12a technology, the existing M. tuberculosis detection methods have solved the problems of low positive rate and long detection time, and achieved immediate and highly sensitive detection effects, suitable for early screening and diagnosis.
CN119753179APending Publication Date: 2025-04-04ZUNYI NO 1 PEOPLES HOSPITAL
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Patent Information
- Application Number
- CN202411850986.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-16
- Publication Date
- 2025-04-04
AI Technical Summary
Technical Problem
The existing Mycobacterium tuberculosis detection methods have problems such as low positive rate, long detection time, expensive equipment and high cost, which are difficult to meet the needs of early screening, diagnosis and prognosis monitoring.
Method used
The CRISPR-Cas12a detection visual biosensing system is used to combine RPA with CRISPR/Cas12a technology, and connect gold@platinum nanoenzyme with ssDNA to magnetic beads to achieve instant and highly sensitive detection results output.
Benefits of technology
It realizes device-free naked eye recognition detection, with good selectivity, repeatability and anti-interference ability, high detection sensitivity and 100% accuracy, and is suitable for early screening and diagnosis of Mycobacterium tuberculosis.
✦ Generated by Eureka AI based on patent content.
Abstract
This scheme discloses a CRISPR-Cas12a sensing system mediated by gold@platinum nanozymes in the field of medical inspection technology and its application in biological detection, wherein the CRISPR-Cas12a sensing system mediated by gold@platinum nanozymes is obtained by reacting a gold@platinum-ssDNA-magnetic bead complex with component A and component B, wherein the gold@platinum-ssDNA-magnetic bead complex is a gold@platinum nanozyme and magnetic beads connected by an ssDNA linker, and the sequence of the ssDNA is shown in SEQ ID NO.1. The gold@platinum nanozyme-mediated CRISPR-Cas12a system is used to detect Mycobacterium tuberculosis, and the gold@platinum nanozyme is connected to the magnetic beads through single-stranded DNA (ssDNA). As an oxidase-like nanozyme, it induces the color change of 3,3',5,5'-tetramethylbenzidine (TMB), which can be identified by the naked eye, has good selectivity, repeatability, good anti-interference ability and accuracy, and has immediate and highly sensitive beneficial technical effects.
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