Rapid lemon yellow detection test strip and application thereof in detection of lemon yellow in feed sample
Through the colloidal gold immunochromatography test strip combined with colloidal gold labeled mouse anti-lemon yellow monoclonal antibody, the existing problems of long-term and cumbersome operation were solved, and the high sensitivity, fast and accurate lemon yellow detection effect was achieved.
CN119757725APending Publication Date: 2025-04-04贵州省兽药饲料检测所(贵州省兽药残留监测中心) +1
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Patent Information
- Application Number
- CN202411667398.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-21
- Publication Date
- 2025-04-04
AI Technical Summary
Technical Problem
The existing methods for detecting lemon yellow are expensive, expensive, time-consuming and cumbersome to operate, making it difficult to achieve rapid large-scale inspections, which limits its application in routine analysis and testing.
Method used
Colloidal gold immunochromatography test strips were used to achieve rapid detection of lemon yellow residue in the sample through the detection line and quality control line on the nitrocellulose membrane.
Benefits of technology
This method has the advantages of high sensitivity, strong specificity, good repeatability and high stability. It can quickly and accurately detect whether there are lemon yellow residues in the sample, and is suitable for screening of large numbers of samples.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention provides a rapid lemon yellow detection test strip and application thereof in detection of lemon yellow in a feed sample, and belongs to the technical field of lemon yellow detection. The colloidal gold immunochromatography test strip for detecting lemon yellow comprises a bottom plate, a nitrocellulose membrane, a gold label pad, a water absorption pad and a sample pad, two ends of the nitrocellulose membrane are respectively lapped with the gold-labeled pad and the water absorption pad, the nitrocellulose membrane is positioned below, the sample pad is lapped with the gold-labeled pad, and the gold-labeled pad is positioned below; a detection line and a quality control line are arranged on the nitrocellulose membrane, the detection line is arranged on one side close to the gold label pad, and the quality control line is arranged on one side close to the water absorption pad; the gold mark pad is coated with a mouse anti-lemon yellow monoclonal antibody marked by 2mu g / mL colloidal gold; the detection line is coated with a lemon yellow antigen; the quality control line is coated with a goat anti-mouse second antibody. The colloidal gold immunochromatography test strip provided by the invention has the advantages of high sensitivity, strong specificity, good repeatability and high stability, and can be used for rapid detection of lemon yellow residues.
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