Mutant Trichoderma fungus deficient in erythritol utilization ability and method for producing target substance using the same
By introducing erythritol-induced promoter into Trichoderma and reducing related gene expression, a mutant strain with a defective erythritol utilization ability was formed, which solved the problem of reducing protein productivity of Trichoderma under high concentration carbon source conditions, and achieved the effect of improving the endogenous accumulation of erythritol and improving the productivity of target substances.
CN119768501APending Publication Date: 2025-04-04KAO CORP
2 Cites 0 Cited by
Patent Information
- Application Number
- CN202380050450.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2022-06-27
- Filing Date
- 2023-06-27
- Publication Date
- 2025-04-04
AI Technical Summary
Technical Problem
In the prior art, Trichoderma bacteria reduce protein productivity due to osmotic pressure stimulation under high concentration carbon source conditions, and the erythritol utilization pathway has not been elucidated.
Method used
By introducing an erythritol-induced promoter in Trichoderma and by reducing or disappearing the expression of relevant genes, mutant strains with erythritol utilization are formed to reduce osmotic pressure stimulation caused by carbon sources in the culture medium.
Benefits of technology
The endogenous accumulation of erythritol in Trichoderma bacteria was achieved, the osmotic pressure stimulation was reduced, the productivity of the target substance was improved, and the target substance could be produced with higher purity and yield.
✦ Generated by Eureka AI based on patent content.
Abstract
The present invention relates to a mutant Trichoderma having a defective ability to utilize erythritol, and the production of a target substance using the mutant Trichoderma. A mutant Trichoderma having a reduced or absent ability to utilize erythritol, wherein in the mutant Trichoderma, the expression of at least one polypeptide selected from the following (A) to (D) is reduced or absent, and a gene encoding a target substance or an enzyme related to its synthesis and an erythritol-inducible promoter connected to the upstream of the gene are introduced: (A) a polypeptide consisting of the amino acid sequence of sequence number 2 or an amino acid sequence having at least 90% identity thereto; (B) a polypeptide consisting of the amino acid sequence of sequence number 4 or an amino acid sequence having at least 90% identity thereto; (C) a polypeptide consisting of the amino acid sequence of sequence number 6 or an amino acid sequence having at least 90% identity thereto; and (D) a polypeptide consisting of the amino acid sequence of sequence number 8 or an amino acid sequence having at least 90% identity thereto.
Need to check novelty before this filing date? Find Prior Art