Detection device for preparing recombinant adenovirus expressing african swine fever protein

Abstract

This invention discloses a detection device for preparing recombinant adenovirus expressing African swine fever protein, relating to the field of biodetection equipment technology. It includes an electrophoresis chamber with a gel plate mounted on it for holding gel. The gel plate has two sliding grooves and two insert plates mounted on it. A well frame is mounted on the gel plate, with its two ends sliding within adjacent sliding grooves. A piston row is slidably connected within the well frame, and a mounting frame is slidably connected to it. The mounting frame also slides within adjacent sliding grooves, and multiple inserts are fixed to the side of the mounting frame away from the well frame. This invention improves the reliability of electrophoresis results by reducing the connectivity area of ​​the wells without changing the gel sample well volume, thus inhibiting sample diffusion outside the wells.

Description

Technical Field

[0001] This invention relates to the field of biological detection equipment technology, and in particular to a detection device for preparing recombinant adenovirus expressing African swine fever protein. Background Technology

[0002] African swine fever (ASF) is an acute, hemorrhagic, and highly contagious disease caused by African swine fever virus (ASFV) infection in domestic pigs and various wild boars. To develop a vaccine against ASF, it is necessary to prepare recombinant adenoviruses that express ASF proteins. The preparation of recombinant adenoviruses mainly includes several key steps: vector construction, recombinant plasmid construction, transfection, virus amplification, purification, and titer determination. Sampling and testing are required at the end of each of these key steps to ensure the safety and effectiveness of the experiment. After constructing the recombinant plasmid, it is necessary to verify the correctness of the plasmid structure. This is achieved by digesting the recombinant plasmid with restriction endonucleases, followed by separating the digested products by agarose gel electrophoresis and comparing them with the expected fragment size, thus verifying the correctness of the plasmid structure.

[0003] Agarose gel electrophoresis is an electrophoresis technique that uses agarose gel as the supporting medium. Based on the principle of molecular sieving, it separates nucleic acid fragments according to their different migration speeds caused by differences in molecular weight or shape. By comparing the migration distance of the separated nucleic acid fragments with that of the expected fragments, it can be determined whether they are the same. After the gel is prepared, it needs to be soaked in electrophoresis buffer to maintain its wetness. Then, the sample (i.e., the separated enzyme digestion product) and the reference (i.e., the expected nucleic acid fragment) are injected into the sample wells. In order to save agarose, the sample wells are usually designed as low rectangular wells. This results in a large area of ​​the sample wells connected to the outside. At the same time, because the density of the sample and the electrophoresis buffer are similar, the sample is prone to diffusion in the electrophoresis buffer, which can cause cross-contamination between the sample and the samples in other sample wells, affecting the final electrophoresis results. Summary of the Invention

[0004] This invention provides a detection device for preparing recombinant adenovirus expressing African swine fever protein, which overcomes the disadvantage that samples are prone to diffusion in the electrophoresis buffer during gel electrophoresis, leading to cross-contamination between adjacent samples and affecting the electrophoresis results.

[0005] The technical implementation of this invention is as follows: A detection device for preparing recombinant adenovirus expressing African swine fever protein includes an electrophoresis box, on which a gel plate is mounted for holding gel. Two sliding grooves are formed on the gel plate, and two insert plates are mounted on the gel plate. An aperture frame is mounted on the gel plate, with its two ends sliding within adjacent sliding grooves. A piston row is slidably connected within the aperture frame. A mounting frame is slidably connected to the aperture frame, with its two ends sliding within adjacent sliding grooves. Multiple inserts are fixed to the side of the mounting frame away from the aperture frame. These inserts form sample loading holes on the gel surface. The inserts are slidably and sealingly connected to the piston row, forming a power chamber in cooperation with the piston row. An elastomer is fixed to the side of the insert away from the aperture frame, expanding the volume inside the sample loading holes on the gel. The inserts and adjacent elastomers form an expansion chamber, and the power chamber communicates with the adjacent expansion chamber.

[0006] Furthermore, a rubber block is fixedly connected inside the sliding groove, and the rubber block is in a limiting fit with the opening frame. The elastic coefficient of the rubber block is greater than that of the elastic body.

[0007] Furthermore, a knob is threaded onto the opening bracket, and the knob engages with the piston pack.

[0008] Furthermore, the width of the sliding groove is equal to the width of the end of the opening bracket and the mounting bracket.

[0009] Furthermore, an open arc block is fixedly connected in the sliding groove, and the open arc block is pressed and engaged with the mounting frame. The vertical distance between the open arc block and the adjacent rubber block is less than the sum of the thicknesses of the end of the open frame and the end of the mounting frame.

[0010] Furthermore, the gel plate is provided with two U-shaped grooves, the insert plate slides in the adjacent U-shaped grooves, a sealing strip is fixedly connected in the U-shaped groove, the sealing strip is pressed and engaged with the adjacent insert plate, a sealing arc block is fixedly connected in the U-shaped groove, and the insert plate is provided with a limiting groove that is limited and engaged with the adjacent sealing arc block.

[0011] Furthermore, the periphery of the sealing strip does not contact the adjacent U-shaped groove.

[0012] Furthermore, the insert plate is slidably connected with symmetrically distributed extrusion members, and an extrusion block is fixedly connected to the insert plate near the adjacent extrusion member. The extrusion block is extruded and engaged with the adjacent extrusion member, and an elastic strip is fixedly connected to the side of the insert plate and the adjacent extrusion member away from the knob.

[0013] Furthermore, the extruder is provided with a pushing part and a supporting part, the supporting part being used to keep adjacent extruders stable.

[0014] Furthermore, it also includes: a limiting strip, which is fixed to the gel plate at a position away from the sliding groove, the limiting strip being coplanar with the side of the adjacent U-shaped groove near the sliding groove, the limiting strip being used to provide support for the gel.

[0015] In summary, this application includes at least one of the following beneficial technical effects: The present invention reduces the connectivity area of ​​the sample loading wells without changing the volume of the gel loading wells, thereby inhibiting sample diffusion outwards and improving the reliability of electrophoresis results; the sliding groove guides the movement of the opening frame and mounting frame, preventing disturbance of the solution and the formation of air bubbles, while also avoiding damage to the sample loading wells, ensuring the accuracy of the final electrophoresis results; the sealing strip fills the gap between the insert plate and the U-shaped groove, thereby improving the sealing performance between the insert plate and the gel plate and preventing solution leakage; by making the width of the sealing strip smaller than the width of the U-shaped groove, water droplets are prevented from accumulating between the sealing strip and the gel plate, facilitating cleaning of the gel plate. Attached Figure Description

[0016] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0017] Figure 2 This is a three-dimensional structural diagram of the gel plate, insert plate, and perforated frame of the present invention;

[0018] Figure 3 Appendix to this invention Figure 2 Enlarged view of point A in the middle;

[0019] Figure 4 This is a three-dimensional cross-sectional view of the elastomer of the present invention during expansion;

[0020] Figure 5 This is a three-dimensional structural diagram of the elastomer of the present invention when it is not expanded;

[0021] Figure 6 This is a three-dimensional structural diagram of the extrusion block and the insert plate of the present invention being coplanar;

[0022] Figure 7 This is a three-dimensional structural diagram of the gel plate and sealing strip of the present invention;

[0023] Figure 8 This is a three-dimensional structural diagram of the extrusion block located inside the insert plate according to the present invention;

[0024] Figure 9 This is a three-dimensional structural diagram of the extruded part of the present invention;

[0025] Figure 10This is a three-dimensional structural diagram of the gel plate and the limiting strip of the present invention.

[0026] The labels in the diagram are as follows: 1-Electrophoresis box, 101-Terminal, 102-Electrode plate, 2-Gel plate, 201-Sliding groove, 202-U-shaped groove, 3-Insertion plate, 4-Opening frame, 5-Piston row, 6-Mounting frame, 7-Insertion block, 701-Power chamber, 8-Elastomer, 801-Expansion chamber, 9-Rubber block, 10-Knob, 11-Opening arc block, 12-Sealing strip, 13-Sealing arc block, 131-Limiting groove, 14-Extrusion part, 141-Pushing part, 142-Supporting part, 15-Extrusion block, 16-Elastic strip, 17-Limiting strip. Detailed Implementation

[0027] The following will be combined with the appendix Figure 1 To be continued Figure 10 This invention will be described in detail, and the technical solutions in the embodiments of this invention will be clearly and completely described. Obviously, the described embodiments are only some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0028] A detection device for preparing recombinant adenovirus expressing African swine fever protein, see [link to relevant documentation]. Figures 1-5 The system includes an electrophoresis box 1, on which a gel plate 2 is mounted for holding gel. The gel plate 2 has two sliding grooves 201 and two insert plates 3. A perforated frame 4 is mounted on the gel plate 2, with both ends sliding within adjacent sliding grooves 201. A piston row 5 is slidably connected within the perforated frame 4. A mounting frame 6 is slidably connected to the perforated frame 4, with both ends sliding within adjacent sliding grooves 201. Multiple insert blocks 7 are fixed to the side of the mounting frame 6 furthest from the perforated frame 4. The insert 7 is used to form a sample application hole on the gel surface. The insert 7 and the piston row 5 are sealed and slidably connected. The insert 7 and the piston row 5 cooperate to form a power chamber 701. An elastomer 8 is fixed to the side of the insert 7 away from the hole holder 4. The elastomer 8 is used to expand the volume inside the sample application hole on the gel. The insert 7 and the adjacent elastomer 8 cooperate to form an expansion chamber 801. The power chamber 701 is connected to the adjacent expansion chamber 801. A rubber block 9 is fixed in the sliding groove 201. The rubber block 9 is limited and cooperated with the hole holder 4. The elastic coefficient of the rubber block 9 is greater than the elastic coefficient of the elastomer 8.

[0029] In the above scheme, the aim is to suppress sample diffusion within the sample well by reducing the opening area of ​​the sample well; terminals 101 and electrodes 102 are installed on both sides of the electrophoresis box 1, and the terminals 101 are electrically connected to the adjacent electrodes 102, and the terminals 101 are used to connect to an external electrophoresis instrument; the gel plate 2 and two insert plates 3 cooperate to form a space for the agarose solution (hereinafter referred to as the solution) to solidify; the lower part of the insert 7 is immersed in the solution, used to form a sample well when the solution solidifies into a gel; the lower side of the piston row 5 consists of multiple elliptical pistons, and the elliptical pistons on the piston row 5 and the adjacent insert 7 can form a sealed fit through rubber rings, etc.; the elastomer 8... The material can be natural latex, etc., which has excellent elasticity and is easy to detach from the gel; the power chamber 701 and the expansion chamber 801 contain a medium, which can be liquid, to ensure the reliability of the transmission; the difference in elastic coefficient between the rubber block 9 and the elastomer 8 can be adjusted by means of material, density, etc. The rubber block 9 can be two sets symmetrically distributed front and back, with two rubber blocks 9 in each set, and the two rubber blocks 9 are symmetrically distributed to improve the uniformity of force when the opening frame 4 is limited by the rubber block 9; by reducing the communication area of ​​the sample well without changing the volume of the gel sample well, the diffusion of the sample to the outside of the sample well is suppressed, thus improving the reliability of the electrophoresis results.

[0030] See Figure 4 and Figure 5 A knob 10 is threadedly connected to the hole holder 4, and the knob 10 is pressed into the piston plate 5.

[0031] In the above scheme, the aim is to change the expansion volume of the elastomer 8 by adjusting the position of the knob 10. The knob 10 consists of a threaded rod and a disc. The side of the disc can be provided with annularly distributed grooves to improve the surface roughness of the disc on the knob 10. When the opening frame 4 moves down, the opening frame 4 drives the knob 10 to move down, and the knob 10 pushes the piston row 5 to move down. The piston row 5 squeezes the medium in the power chamber 701 into the adjacent expansion chamber 801, causing the elastomer 8 to expand. After the piston row 5 stops moving, the volume that the elastomer 8 can reach is referred to as the set volume.

[0032] The steps for adjusting the volume of the elastomer 8 by changing the position of knob 10 are as follows: Initially, the upper side of the piston plate 5 is in contact with the threaded rod of knob 10. At this time, when the orifice holder 4 moves, it directly drives the piston plate 5 to move. The moving distance of the piston plate 5 is equal to the moving distance of the orifice holder 4. The volume of the elastomer 8 after expansion is at its maximum. When it is necessary to reduce the volume of the sample loading hole, simply rotate knob 10 to create a gap between the threaded rod of knob 10 and the piston plate 5. During the downward movement of the orifice holder 4, knob 10 is first driven to contact the piston plate 5, and then the piston plate 5 is pushed downward, reducing the moving distance of the piston plate 5. That is, the volume of the medium entering the expansion chamber 801 is reduced, and the volume of the elastomer 8 after expansion is reduced. Thus, the volume of the sample loading hole can be changed by rotating knob 10.

[0033] See Figure 3 The width of the sliding groove 201 is equal to the width of the ends of the opening bracket 4 and the mounting bracket 6.

[0034] In the above scheme, the purpose is to limit the opening frame 4 and the mounting frame 6 by the sliding groove 201, keep the direction of movement of the opening frame 4 and the mounting frame 6 unchanged, and avoid damage to the gel; the ends of the opening frame 4 and the mounting frame 6 refer to the front and rear ends of the opening frame 4 and the mounting frame 6; by guiding the opening frame 4 and the mounting frame 6 by the sliding groove 201, the opening frame 4 and the mounting frame 6 move along the sliding groove 201, which makes it easier to smoothly insert the insert 7 into the solution, reduce the probability of generating bubbles in the solution, and make it easier to smoothly pull the insert 7 out of the gel after the gel is formed, avoiding damage to the sample well due to swinging or other reasons during the process of pulling the insert 7 out of the gel, and accelerating the cross-contamination of the sample in the sample well.

[0035] See Figure 3 An open arc block 11 is fixedly connected inside the sliding groove 201. The open arc block 11 is pressed and engaged with the mounting frame 6. The vertical distance between the open arc block 11 and the adjacent rubber block 9 is less than the sum of the thicknesses of the end of the open frame 4 and the end of the mounting frame 6.

[0036] The above solution aims to address the problem that the liquid level at the contact point with the insert 7 is lower than the liquid level at other locations due to the surface tension and viscosity of the solution. During the insertion of the insert 7 into the solution, the viscosity of the solution causes the contact point to be lower than the liquid level at other locations. Over time, the solution gradually wets other parts of the insert 7 under its own gravity until the contact point is level with the liquid level (e.g., a wooden stick inserted into cement). However, as the temperature of the solution gradually decreases, the solution gradually transitions to a gel state. The change causes the solution to gradually reduce the wetting speed of the insert 7, which easily leads to the height of the sample well edge being lower than the height of the gel after the solution forms a gel. The open arc block 11 is made of elastic metal and can undergo slight deformation when the open arc block 11 is squeezed, so that the insert 7 is inserted into the solution to a certain depth, increasing the height of the solution wetting on the surface of the insert 7. Then the insert 7 is pulled out to the above depth, eliminating the step of the solution gradually wetting the insert 7 after the insert 7 comes to rest. In this way, the concave liquid surface formed at the contact point between the solution and the insert 7 due to surface tension is eliminated, and the uniformity of the gel thickness is improved.

[0037] The operation steps of the above scheme are as follows: During gel electrophoresis, the two insert plates 3 are installed in the corresponding positions on the gel plate 2. Then, the prepared solution is poured into the space formed by the gel plate 2 and the two insert plates 3 and left to solidify. During the solidification period, the perforated frame 4, piston row 5, mounting frame 6, insert block 7, and elastomer 8 are moved towards the sliding groove 201. The mounting frame 6 and the perforated frame 4 enter the sliding groove 201 in sequence and squeeze the rubber block 9 in sequence. Then, the mounting frame 6 contacts the perforated arc block 11 and stops moving. When both the insert block 7 and the elastomer 8 stop moving, and the solution completely submerges the elastomer 8, the perforation frame 4 continues to move downward. The perforation frame 4 pushes the piston row 5 downward through the knob 10. The piston row 5 moves downward relative to the multiple insert blocks 7, squeezing the medium in the power chamber 701 into the adjacent expansion chamber 801, causing all the elastomers 8 to expand until the end of the perforation frame 4 contacts the end of the mounting frame 6. At this time, the perforation frame 4 stops relative to the mounting frame 6 (at this time, the elastomer 8 expands to the set volume, and the perforation frame 4 passes over the rubber block 9).

[0038] Continue pressing the perforation frame 4 downwards, causing it to press against the perforation arc block 11 via the mounting bracket 6. The perforation arc block 11 deforms a short distance, and simultaneously, the mounting bracket 6 drives the insertion block 7 to move downwards, increasing the height at which the solution wets the surface of the insertion block 7. At this point, release the perforation frame 4. Under the resetting action of the perforation arc block 11, the perforation frame 4 and the mounting bracket 6 move upwards until the perforation arc block 11 resets and the perforation frame 4 is stopped by the rubber block 9. At the same time, the insertion block 7 moves upwards. At this point, the height of the solution adhering to the insertion block 7 is higher than the liquid surface, eliminating the step of the solution gradually wetting the insertion block 7 and accelerating the speed at which the liquid surface returns to a flat state. After the agarose solution forms a gel, remove the two insertion plates 3 and lift the perforation frame 4. First, the medium in the expansion chamber 801 is driven by the piston plate 5 into the adjacent power chamber 701, causing the elastomer 8 to reset. Then, the opening frame 4 drives the mounting frame 6 to move upward, and the transmission plug 7 loses contact with the gel. At this time, a sample well is formed on the gel surface with a connected area equal to the area of ​​the lower side of the plug 7. Electrophoresis buffer is injected into the electrophoresis box 1, and the electrophoresis buffer covers the gel. Then, the enzyme digestion products and the control sample (i.e. the expected fragment) are injected into different sample wells using a pipette, and electrophoresis is performed on them. By utilizing the different movement speeds of enzyme digestion products of different lengths in the gel due to the influence of current, enzyme digestion products (i.e. nucleic acid fragments) of different lengths are separated. Finally, the gel is removed and the electrophoresis results are observed through a gel imaging instrument.

[0039] See Figure 2 , Figure 6 and Figure 7 The gel plate 2 is provided with two U-shaped grooves 202. The insert plate 3 slides in the adjacent U-shaped grooves 202. A sealing strip 12 is fixed in the U-shaped groove 202. The sealing strip 12 is squeezed and matched with the adjacent insert plate 3. A sealing arc block 13 is fixed in the U-shaped groove 202. The insert plate 3 is provided with a limiting groove 131 that matches the adjacent sealing arc block 13.

[0040] In the above scheme, the aim is to improve the sealing performance between the insert plate 3 and the gel plate 2; the sealing strip 12 is made of flexible rubber, and the sealing arc block 13 is made of elastic metal; when the insert plate 3 is inserted into the corresponding U-shaped groove 202, the insert plate 3 squeezes the adjacent sealing strip 12 to deform, and the sealing strip 12 fills the gap between the insert plate 3 and the U-shaped groove 202, thereby improving the sealing performance between the insert plate 3 and the gel plate 2. As the insert plate 3 moves down, the insert plate 3 squeezes the adjacent sealing arc block 13 to deform until the upper side of the insert plate 3 is coplanar with the upper side of the gel plate 2. At this time, the limiting groove 131 on the insert plate 3 corresponds to the adjacent sealing arc block 13. At this time, the sealing arc block 13 resets under its own elasticity and limits the adjacent insert plate 3.

[0041] See Figure 7 The periphery of the sealing strip 12 does not contact the adjacent U-shaped groove 202.

[0042] In the above design, the sealing strip 12 and the gel plate 2 are designed to facilitate cleaning after electrophoresis; multiple through holes communicating with the outside can be provided on the lower side of the U-shaped groove 202 to facilitate the drainage of residual water droplets after cleaning; the periphery of the sealing strip 12 does not contact the gel plate 2, that is, there are gaps between the sealing strip 12 and the gel plate 2 except for the two ends (see Appendix). Figure 7 This prevents water droplets from accumulating between the sealing strip 12 and the gel plate 2, making it easier to clean the gel plate 2.

[0043] See Figure 6 and Figure 8 The insert plate 3 is slidably connected with symmetrically distributed extrusion parts 14. An extrusion block 15 is fixedly connected to the insert plate 3 near the adjacent extrusion parts 14. The extrusion block 15 is extruded and engaged with the adjacent extrusion parts 14. An elastic strip 16 is fixedly connected to the side of the insert plate 3 and the adjacent extrusion parts 14 away from the knob 10.

[0044] In the above scheme, the aim is to reduce the friction between the insert plate 3 and the sealing strip 12 and extend the service life of the sealing strip 12; there can be multiple extrusion blocks 15 to improve the uniformity of force on the extrusion member 14; the sealing strip 12 forms two vertical parts and one horizontal part in the adjacent U-shaped groove 202; when the insert plate 3 moves down along the adjacent U-shaped groove 202, the elastic strip 16 and the extrusion member 14 first contact the horizontal part of the sealing strip 12, and as the insert plate 3 continues to move down, the extrusion member 14 moves up relative to the adjacent insert plate 3, and the extrusion member 14 is subjected to the extrusion action of the adjacent extrusion blocks 15. The insert plate 3 moves downward toward the adjacent vertical portion of the sealing strip 12 and eventually contacts the sealing strip 12, compressing and deforming the vertical portion of the sealing strip 12. The compressed sealing strip 12 deforms and fills the gap between the front and rear sides of the insert plate 3 and the adjacent U-shaped groove 202 (the side of the final extrusion member 14 away from the adjacent extrusion block 15 is coplanar with the side of the adjacent insert plate 3). By reducing the contact area between the insert plate 3 and the sealing strip 12 when the insert plate 3 moves downward, the compressive force on the vertical portion of the sealing strip 12 is reduced, thereby reducing the wear on the sealing strip 12 and extending the service life of the sealing strip 12.

[0045] See Figure 9 The extrusion member 14 is provided with a pushing part 141 and a supporting part 142. The supporting part 142 is used to keep adjacent extrusion members 14 stable.

[0046] In the above scheme, the aim is to improve the stability of the insert plate 3. During the upward movement of the extrusion member 14 relative to the extrusion block 15, the extrusion block 15 first contacts the pushing part 141 (at this time, due to the effect of the inclined surface, the extrusion block 15 is subjected to an upward force given by the extrusion member 14, which increases the force exerted by the insert plate 3 on the adjacent sealing arc block 13). When the extrusion member 14 moves away from the side of the adjacent extrusion block 15 and is coplanar with the side of the adjacent insert plate 3, the extrusion block 15 contacts the support part 142. At this time, the extrusion block 15 is no longer subjected to an upward force, thereby reducing the force exerted by the insert plate 3 on the adjacent sealing arc block 13 and improving the stability of the insert plate 3.

[0047] See Figure 10 It also includes: a limiting strip 17, which is fixed to the gel plate 2 at a position away from the sliding groove 201. The limiting strip 17 is coplanar with the side of the adjacent U-shaped groove 202 near the sliding groove 201. The limiting strip 17 is used to provide support for the gel.

[0048] The above solution aims to improve the stability of the gel when injecting electrophoresis buffer. Currently, when injecting electrophoresis buffer into electrophoresis chamber 1, it is necessary to repeatedly pour the buffer onto both sides of chamber 1 to prevent the buffer from flowing between them, thus reducing the impact of pouring on the gel position. This method is cumbersome and not conducive to improving the electrophoresis rate. This solution uses a limiting strip 17 and an insert block 7 to fix the gel together, preventing the flow of the electrophoresis buffer from affecting the gel position. Therefore, only one side of the electrophoresis buffer needs to be poured into the chamber 1, saving operational steps. The height of the limiting strip 17 and the height of the sample well should be less than the thickness of the gel to avoid affecting the movement of the sample within the gel. After the solution forms a gel, remove the two insert plates 3 (at this time, there is no need to remove the insert 7). Then pour the electrophoresis buffer to the left side of the electrophoresis box 1. The electrophoresis buffer will cover the gel and flow to the right side of the electrophoresis box 1 through the gaps between the multiple inserts 7. During this process, the multiple inserts 7 and the limiting strip 17 together provide support for the gel to prevent the gel from moving when the electrophoresis buffer flows. When the electrophoresis buffer completely covers the gel, remove the insert 7 and start electrophoresis.

[0049] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A detection device for preparing recombinant adenovirus expressing African swine fever protein, characterized in that, It includes an electrophoresis box (1), on which a gel plate (2) is installed. The gel plate (2) is used to place gel. Two sliding grooves (201) are opened on the gel plate (2), and two insert plates (3) are installed on the gel plate (2). An aperture holder (4) is installed on the gel plate (2). Both ends of the aperture holder (4) slide within adjacent sliding grooves (201). A piston row (5) is slidably connected within the aperture holder (4). A mounting bracket (6) is slidably connected to the aperture holder (4). Both ends of the mounting bracket (6) slide within adjacent sliding grooves (201). Multiple inserts (7) are fixed to the side of the mounting bracket (6) away from the aperture holder (4). The inserts (7) are used to form sample loading holes on the gel surface. The inserts (7) are connected to the gel plate (2). The piston row (5) is sealed and slidably connected. The insert (7) cooperates with the piston row (5) to form a power chamber (701). An elastomer (8) is fixed to the side of the insert (7) away from the opening frame (4). The elastomer (8) is used to expand the volume inside the sample well on the gel. The insert (7) cooperates with the adjacent elastomer (8) to form an expansion chamber (801). The power chamber (701) is connected to the adjacent expansion chamber (801). The power chamber (701) and the expansion chamber (801) contain a medium. An open arc block (11) is fixedly connected in the sliding groove (201). The open arc block (11) is pressed and engaged with the mounting frame (6). The vertical distance between the open arc block (11) and the adjacent rubber block (9) is less than the sum of the thicknesses of the end of the open frame (4) and the end of the mounting frame (6). A rubber block (9) is fixedly connected inside the sliding groove (201). The rubber block (9) is in a limiting fit with the opening frame (4). The elastic coefficient of the rubber block (9) is greater than that of the elastic body (8). A knob (10) is threaded onto the opening frame (4), and the knob (10) is press-fitted with the piston row (5).

2. The detection device for preparing recombinant adenovirus expressing African swine fever protein according to claim 1, characterized in that, The width of the sliding groove (201) is equal to the width of the ends of the opening bracket (4) and the mounting bracket (6).

3. The detection device for preparing recombinant adenovirus expressing African swine fever protein according to claim 1, characterized in that, The gel plate (2) is provided with two U-shaped grooves (202), the insert plate (3) slides in the adjacent U-shaped grooves (202), a sealing strip (12) is fixed in the U-shaped groove (202), the sealing strip (12) is squeezed and cooperated with the adjacent insert plate (3), a sealing arc block (13) is fixed in the U-shaped groove (202), and a limiting groove (131) is provided on the insert plate (3) to limit and cooperate with the adjacent sealing arc block (13).

4. The detection device for preparing recombinant adenovirus expressing African swine fever protein according to claim 3, characterized in that, The periphery of the sealing strip (12) does not contact the adjacent U-shaped groove (202).

5. The detection device for preparing recombinant adenovirus expressing African swine fever protein according to claim 3, characterized in that, The insert plate (3) is slidably connected with symmetrically distributed extrusion parts (14). An extrusion block (15) is fixedly connected to the insert plate (3) near the adjacent extrusion parts (14). The extrusion block (15) is extruded and engaged with the adjacent extrusion parts (14). An elastic strip (16) is fixedly connected to the side of the insert plate (3) and the adjacent extrusion parts (14) away from the knob (10).

6. The detection device for preparing recombinant adenovirus expressing African swine fever protein according to claim 5, characterized in that, The extrusion member (14) is provided with a pushing part (141) and a supporting part (142), the supporting part (142) being used to keep adjacent extrusion members (14) stable.

7. The detection device for preparing recombinant adenovirus expressing African swine fever protein according to claim 3, characterized in that, It also includes: A limiting strip (17) is fixed to the gel plate (2) at a position away from the sliding groove (201). The limiting strip (17) is coplanar with the side of the adjacent U-shaped groove (202) near the sliding groove (201). The limiting strip (17) is used to provide support for the gel.

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