Oligonucleotide and functionalized AuNPs for detecting helicobacter pylori as well as preparation method and application of oligonucleotide and functionalized AuNPs

By screening and functionalizing the binding of oligonucleotide sequences to gold nanoparticles, the existing cumbersome detection methods of Helicobacter pylori are solved, and fast and easy-to-operate detection is achieved, and the specificity and sensitivity of the detection are improved.

CN119859630APending Publication Date: 2025-04-22INDICATE BIOTECHNOLOGY (SHANGHAI) CO LTD
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Patent Information

Application Number
CN202510071029.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-16
Publication Date
2025-04-22

AI Technical Summary

Technical Problem

The existing Helicobacter pylori detection methods are cumbersome and cannot achieve fast and easy-to-operate detection. In selecting oligonucleotide sequences, biosensing methods require hundreds of experiments to verify specificity and sensitivity.

Method used

By screening the 23SrRNA gene sequence of Helicobacter pylori using the Gene bank database, combining Oligarch software and scikit-learn for energy comparison and prediction, the oligonucleotide sequence that is most suitable for detection of Helicobacter pylori was screened out, and bound to the surface of gold nanoparticles to form functionalized AuNPs.

Benefits of technology

It realizes rapid detection of naked-eye observation results in less than half an hour, reduces the number of experiments, improves the specificity and sensitivity of the detection, and avoids the cumbersome operations of traditional methods.

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Abstract

The invention discloses oligonucleotide for detecting helicobacter pylori, functionalized AuNPs and a preparation method and application of the oligonucleotide. The sequence of the oligonucleotide is as shown in SEQ ID NO: 1-42. According to the invention, the specific oligonucleotide sequence with high specificity and sensitivity for detecting the helicobacter pylori is found by utilizing Oligarch software, and multiple experiments are carried out, so that the sequence can be theoretically used for detecting the helicobacter pylori through verification, and the necessity of verification by multiple experiments is solved. Specific oligonucleotides are combined with the surfaces of the gold nanoparticles, so that the gold nanoparticles are endowed with recognition and pairing capabilities for target helicobacter pylori nucleic acid, and a result can be directly observed by naked eyes within less than half an hour.
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