SNP molecular markers associated with bovine sperm deformity rate based on HNRNPA2B1 gene and their application
By detecting the SNP site polymorphism of the bovine HNRNPA2B1 gene, a haplotype combination with low sperm malformation rate was screened out, which solved the problem of difficulty in evaluating bovine sperm malformation rate in the prior art, and achieved efficient and low-cost breeding guidance.
CN120060502BActive Publication Date: 2025-08-12INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI +1
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Patent Information
- Application Number
- CN202510550887.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-29
- Publication Date
- 2025-08-12
- Estimated Expiration
- 2045-04-29
AI Technical Summary
Technical Problem
There is a lack of effective means in the prior art to evaluate and improve the rate of sperm abnormalities of cows, affecting the breeding effect of male livestock for dairy crops.
Method used
By detecting the polymorphisms of the SNP1 and SNP2 loci of the bovine HNRNPA2B1 gene, specific primers were designed for PCR reactions, sequencing to determine the genotype, and screening out haplotype combinations with low sperm malformation rates to evaluate bull sperm mobility.
Benefits of technology
It achieves efficient and low-cost evaluation of bull sperm abnormality rates, provides reliable breeding guidance, and improves the reliability and efficiency of breeding high-quality next generations.
✦ Generated by Eureka AI based on patent content.
Abstract
The present invention discloses a bovine sperm deformity rate-associated SNP molecular marker based on the HNRNPA2B1 gene and its application, belonging to the field of genetic molecular biology technology. There are two SNP molecular markers: SNP1 is located at the cattle genome chr4:69733358 position, with a polymorphism of C or A; SNP2 is located at the cattle genome chr4:69733387 position, with a polymorphism of T or G. The two SNP sites provided by the present invention, g.-1713A>C and g.-1684G>T, have dominant genotypes of AA and GG, respectively, and their semen collection volume and sperm deformity rate have better performance. By detecting the polymorphisms of these two SNP sites, bull sperm motility assessment can be achieved, thereby assisting breeding and providing reliable support for breeding high-quality next-generation breeding, with advantages such as high reliability, high efficiency, and low cost.
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