Method for inducing triploidy in abalone haliotis discus hannai by salinity

By employing salinity gradient treatment and re-osmosis technology, the problem of low triploid induction efficiency in wrinkled abalone has been solved, achieving efficient, stable, and environmentally friendly triploid induction, and promoting the sustainable development of the abalone farming industry.

CN120092755BActive Publication Date: 2025-12-26XIAMEN UNIV
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Patent Information

Application Number
CN202510520291.5
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-04-24
Publication Date
2025-12-26
Estimated Expiration
2045-04-24

AI Technical Summary

Technical Problem

In existing technologies, the induction efficiency of triploid abalone is low and unstable. Chemical drug residues and expensive equipment limit its application in large-scale production, and salinity induction technology is not yet mature.

Method used

By simultaneously fertilizing and then treating the fertilized eggs of *Abalone fasciatus* with high or low salinity, and combining this with salinity gradient sand filtration and seawater re-infiltration treatment, the osmotic pressure is gradually adjusted to reduce damage to the fertilized eggs, thus achieving efficient induction of triploidy in *Abalone fasciatus*.

Benefits of technology

It achieved a 100% triploid induction rate, avoiding chemical drug residues and the use of expensive equipment, reducing production costs, meeting the requirements of green aquaculture, and improving induction efficiency and stability.

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Abstract

The application discloses a method for inducing triploid of Haliotis discus hannai Ino by salinity, comprising the following steps: (1) synchronously fertilizing sperm and ovum of Haliotis discus hannai Ino to obtain fertilized eggs; (2) when the proportion of the first polar body appearing in the fertilized eggs obtained in step (1) is 60-70%, the fertilized eggs are subjected to high-salt treatment or low-salt treatment; and (3) the fertilized eggs treated in step (2) are subjected to reperfusion treatment of salinity gradient sand-filtered seawater, so as to gradually adjust the osmotic pressure by the salinity gradient sand-filtered seawater, slow down the damage to the fertilized eggs, and then resuspend in fresh sand-filtered seawater with normal salinity, and perform conventional incubation and cultivation. The application can realize efficient induction of triploid by optimizing the salinity and reperfusion steps, avoids the problems of drug residues and equipment requirements in traditional chemical and physical methods, and has the advantages of safety, simplicity and high efficiency.
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