A thawing auxiliary insemination device for bovine frozen semen and an insemination method thereof

Abstract

The application belongs to the technical field of insemination, and particularly relates to a thawing auxiliary insemination device for bull frozen semen insemination and an insemination method thereof, which comprises the following steps: a box cover is hingedly connected to the upper portion of a freezing box, a rotating shaft is arranged on a thawing frame, an end portion of the rotating shaft is connected with a heat preservation frame, a warm water space is left between the heat preservation frame and the thawing frame, the warm water space has a closed state and an open state which are changed with the rotation of the heat preservation frame, a servo motor is installed on the heat preservation frame, a control console is electrically connected with the servo motor, a support block is connected with a support ring in the warm water space on one side of the support block, and the support ring is used for supporting a bull frozen semen fine tube. The thawing frame in the warm water is preserved by the heat preservation frame, so that the temperature of the water liquid is prevented from decreasing too fast, the control console controls the servo motor to drive the support ring to reciprocatingly swing the bull frozen semen fine tube in the warm water space, so that the step of manual shaking is omitted, the time is accurately controlled, and the swinging thawing operation of the bull frozen semen fine tube is automatically completed.

Description

Technical Field

[0001] This invention belongs to the field of breeding technology, and more specifically, relates to a thawing-assisted breeding device for bovine frozen semen and its breeding method. Background Technology

[0002] Frozen semen insemination is a widely used reproductive management method in modern animal husbandry. It involves freezing and preserving bull semen, which can then be thawed and used for artificial insemination of cows when needed, thereby improving reproductive efficiency and genetic quality control.

[0003] Freezing process: Highly motile and morphologically normal sperm are selected and then undergo a series of dilution, cooling and cryoprotectant addition steps, and finally frozen and preserved at extremely low temperatures (usually liquid nitrogen temperature, -196°C). The containers used to preserve bovine frozen semen are usually 0.25 ml frozen semen tubes (some are 0.5 ml frozen semen tubes).

[0004] Thawing process: Remove the frozen sperm tubes from the container and let them stand in the air for 3-5 seconds. Then place them in warm water at 38℃-40℃ and gently shake for 15-30 seconds to thaw (to promote even thawing and improve sperm motility). Then cut them open for insemination.

[0005] Most of the current freezing containers are liquid nitrogen tanks. The frozen bovine sperm tubes are placed in the liquid nitrogen tank to freeze, and thawing is done by placing the frozen bovine sperm tubes in a basin of warm water. During thawing, the frozen bovine sperm tubes need to be manually taken out and placed in the basin and shaken, but the water temperature drops quickly and the workers are prone to fatigue. Summary of the Invention

[0006] The purpose of this invention is to overcome the shortcomings of the prior art and provide a thawing-assisted insemination device and insemination method for bovine frozen semen.

[0007] To address the aforementioned technical problems, this invention provides a thawing-assisted insemination device for bovine frozen semen, comprising: a freezing chamber, a control console, a lid, a thawing frame, a rotating shaft, a heat-insulating frame, a servo motor, a support block, and a support ring. The upper part of the freezing chamber is equipped with the control console, the lid, and the thawing frame. The control console has a built-in controller. The lid is hinged to the upper part of the freezing chamber, and a handle is installed on the upper side of the lid. A rotating shaft is rotatably mounted on the thawing frame, and the rear end of the rotating shaft is connected to the heat-insulating frame. A space suitable for storing 38℃-40℃ warm water is left between the heat-insulating frame and the thawing frame. The warm water space for insulation has a closed and open state that changes with the rotation of the insulation frame; both the thawing frame and the insulation frame are made of heat-insulating material, or the inner wall of the thawing frame and the outer wall of the insulation frame are covered with heat-insulating stickers; an electric heater located in the thawing space is installed on the insulation frame, and a servo motor is installed on the insulation frame. The control console is electrically connected to the servo motor, and a support block is connected to the output shaft of the servo motor. Two sets of support rings, which are left and right opposite each other and located in the warm water space, are connected to one side of the support block. The support rings are used to support the frozen fine tubes.

[0008] Preferably, it further includes: a signal transmitter, a signal receiver, a gear and an arc rack. The signal transmitter is mounted on the rotating shaft, and the signal receiver located directly below the signal transmitter is mounted on the defrosting frame. The signal receiver is adapted to receive the signal emitted from the signal transmitter. The signal receiver is electrically connected to the control console. The front end of the rotating shaft is connected to the gear, and the hinge of the box cover is connected to the arc rack that meshes with the gear.

[0009] Preferably, it further includes: a support frame and a tube rack. There are two lids, which are opposite each other. The freezer is provided with two support frames that are opposite each other, which correspond to the two lids respectively. Each support frame is provided with a tube rack. The tube rack has a support rod one and a support rod two. The upper part of the support rod one of the two tube racks is respectively provided with a slot one suitable for accommodating 0.25mm frozen beef fine tubes and a slot two suitable for accommodating 0.5mm frozen beef fine tubes. The upper part of the support rod two of the two tube racks is respectively provided with a slot two suitable for accommodating 0.25mm frozen beef fine tubes and a slot two suitable for accommodating 0.5mm frozen beef fine tubes.

[0010] Preferably, it also includes: insulated windows and doors, two insulated windows and doors hinged on the freezer, each corresponding to a thin tube rack, the thin tube racks being slidably placed on the upper side of the support frame, and the upper side of the thin tube racks having slots suitable for placing other thin tube racks on the lower side.

[0011] Preferably, it further includes: a slide rod, a clamping plate, a compression spring, and a top rod. The support frame is slidably disposed inside the freezer. A slide rod is slidably disposed on one side of the tube rack. The first end of the slide rod is connected to a clamping plate that slides with the tube rack. A compression spring is connected between the clamping plate and the tube rack and surrounds the slide rod. The compression spring is used to reset the clamping plate after sliding. The second end of the slide rod is wedge-shaped. A top rod suitable for squeezing the second end of the slide rod is disposed inside the freezer.

[0012] Preferably, it further includes: a wedge-shaped limiting block, a connecting rod, a V-shaped lever, a torsion spring, and an L-shaped lever. The wedge-shaped limiting block is slidably provided on the support frame. A connecting spring located inside the support frame connects the lower side of the wedge-shaped limiting block to the support frame. A connecting rod that slides on the support frame is connected to one side of the wedge-shaped limiting block. A V-shaped lever for moving the connecting rod is rotatably provided inside the freezer. A torsion spring located inside the freezer connects the V-shaped lever to the freezer. An L-shaped lever for moving the V-shaped lever is connected to the hinge of the insulated window door.

[0013] Preferably, it also includes: a support plate, a pressure rod, and a connecting rod. The slot on the support rod is L-shaped. The upper part of the freezer is connected to the support plate, and the lower part of the support plate is connected to the pressure rod located above the support rod. The pressure rod is adapted to press down to lift the frozen beef jelly tube from the slot on the support rod. A connecting rod is rotatably provided between the lid and the support frame.

[0014] Preferably, it also includes: a second support plate and a limiting frame, two opposing second support plates are connected to the freezer, the second support plate is connected to the limiting frame, and the limiting frame has multiple through slots for the raised bovine frozen fine tube to pass through.

[0015] Preferably, it further includes: a baffle plate and a second connecting rod, a second support plate blocking the top of the second support rod, a baffle plate rotatably provided inside the freezer, a second connecting rod rotatably provided between the support frame and the baffle plate, the baffle plate having an extended state and a retracted state as the support frame moves up and down, and the baffle plate blocking the bottom of the first support rod when in the extended state.

[0016] This invention also provides a breeding method comprising: first, selecting highly motile and morphologically normal sperm, diluting them, and then filling them into 0.25mm frozen bovine sperm tubes, with only 0.22mm of the tube filled, leaving 0.03mm of air to allow for thermal expansion and contraction and prevent tube bursting; if using 0.5mm frozen bovine sperm tubes, only 0.44mm is filled, leaving 0.06mm of air. This filling method can be adjusted according to actual conditions to prevent the frozen bovine sperm tubes from bursting due to thermal expansion and contraction; then freezing the frozen bovine sperm tubes in a freezer at -196°C; when using, removing the frozen bovine sperm tubes from the freezer and automatically thawing them in a thawing frame using a servo motor to power the thawing, eliminating the need for manual thawing; after thawing, artificial insemination of the cow with the sperm from the frozen bovine sperm tubes to complete the breeding process.

[0017] The beneficial effects that this invention can achieve by overcoming the shortcomings of the prior art include:

[0018] 1. The warm water in the defrosting box is kept warm by the insulation frame to prevent the water temperature from dropping too quickly. The control console controls the servo motor to drive the support ring to make the frozen bovine fine tube swing back and forth in the warm water space, thus eliminating the need for manual shaking, accurately controlling the time, and automating the swing defrosting operation of the frozen bovine fine tube.

[0019] 2. Two thin tube racks are used to hold 0.25mm and 0.5mm beef jelly tubes respectively, which makes it easy to distinguish them and allows for multiple uses. In addition, the two lids can be opened and closed separately as needed to prevent excessive leakage of cold air from the freezer.

[0020] 3. When opening the box to take out the frozen beef fine tubes, the steps of moving the tube holder upward, loosening the clamp, and lifting the frozen beef fine tubes are interconnected and coordinated, with high synchronization, which not only ensures stability but also makes it easy to take out the frozen beef fine tubes. Attached Figure Description

[0021] Figure 1 This is an assembly diagram of the present invention.

[0022] Figure 2 This is a schematic diagram of the box cover, gear, and arc-shaped rack of the present invention, which also includes a fully sectional thawing frame.

[0023] Figure 3 This is a cross-sectional view of the insulation frame of the present invention.

[0024] Figure 4 This is a bottom view of the support block of the present invention.

[0025] Figure 5This is a cross-sectional view of the freezer of the present invention.

[0026] Figure 6 This is a schematic diagram of the existing frozen fine tube and the support frame and fine tube holder of the present invention.

[0027] Figure 7 This is an exploded view of the support frame and thin tube frame of the present invention.

[0028] Figure 8 This is a schematic diagram of the slide bar and clamping plate of the present invention.

[0029] Figure 9 This is a schematic cross-sectional view of support rod one and support rod two of the present invention.

[0030] Figure 10 This is a schematic diagram showing the state of multiple thin tube frames stacked together according to the present invention.

[0031] Figure 11 For the present invention Figure 5 Enlarged view of point A.

[0032] Figure 12 This is a schematic diagram of the freezer and insulated window door of the present invention, wherein the freezer is fully cut out.

[0033] Figure 13 For the present invention Figure 12 Enlarged view of point B.

[0034] Figure 14 This is a schematic diagram showing the positional relationship between the V-shaped lever and the L-shaped lever of the present invention.

[0035] Figure 15 This is a rear view of the lid of the box according to the present invention, wherein the lid on the left is in the open state and the lid on the right is in the closed state.

[0036] The reference numerals in the accompanying drawings provided by this invention are as follows: 01-frozen fine tube, 1-freezing box, 11-insulated window door, 12-control console, 2-box lid, 3-thawing frame, 30-heater, 31-rotating shaft, 32-insulated frame, 33-support block, 34-support ring, 35-servo motor, 36-signal transmitter, 37-signal receiver, 38-gear, 39-arc rack, 41-support frame, 42-fine tube frame, 43-support rod one, 44-support rod two, 51-slide rod, 52-clamping plate, 53-compression spring, 54-top rod, 61-wedge-shaped limiting block, 62-connecting rod, 63-V-shaped lever, 64-torsion spring, 65-L-shaped lever, 71-support plate one, 72-pressure rod, 73-connecting rod one, 74-support plate two, 75-limiting frame, 81-baffle plate, 82-connecting rod two. Detailed Implementation

[0037] In the description of this invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0038] Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance. The technical solutions of the present invention will now be clearly and completely described in conjunction with the accompanying drawings. It should be understood that the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0039] A thawing-assisted insemination device for bovine frozen semen, such as... Figures 1-4As shown, the system includes: a freezer 1, a control console 12, a lid 2, a defrosting frame 3, a rotating shaft 31, an insulation frame 32, a servo motor 35, a support block 33, and a support ring 34. The freezer 1 has a control console 12, a lid 2, and a defrosting frame 3 on its upper part. The control console 12 has a built-in controller. The lid 2 is hinged to the upper part of the freezer 1, and a handle is installed on the upper side of the lid 2. The lid 2 can be opened by rotating the handle. The defrosting frame 3 has a rotating shaft 31, and the rear end of the rotating shaft 31 is connected to the insulation frame 32. A warm water space suitable for storing and maintaining 38℃-40℃ warm water is left between the insulation frame 32 and the defrosting frame 3. The warm water space has a closed state and an open state that change with the rotation of the insulation frame 32. The warm water space enhances the heat preservation effect of the warm water and prevents contamination. Rotating the insulation frame 32 ninety degrees to make it vertical opens the warm water space, allowing warm water to be added. Both the thawing frame 3 and the insulation frame 32 are made of heat-insulating material, or the inner wall of the thawing frame 3 and the outer wall of the insulation frame 32 are covered with heat-insulating stickers, further enhancing the heat preservation of the water. Specifically, in this embodiment, both the thawing frame 3 and the insulation frame 32 are made of heat-insulating material. An electric heater 30 is installed on the insulation frame 32 within the thawing space. The electric heater 30 heats the water in the warm water space, and the control panel 12 can control the heating temperature of the electric heater 30, thereby precisely controlling the water temperature in the warm water space and preserving its warmth. A servo motor 35 is mounted on the temperature frame 32. The control console 12 is electrically connected to the servo motor 35. A support block 33 is connected to the output shaft of the servo motor 35. Two sets of support rings 34, which are opposite each other and located in the warm water space, are connected to one side of the support block 33. The support rings 34 are used to support the frozen bovine semen tube 01. After the frozen bovine semen tube 01 stored in the freezer 1 is taken out, the servo motor 35 is controlled by the control console 12 to drive the support block 33 to rotate the support rings 34 180°, so that the support rings 34 rotate out of the warm water space. Then the frozen bovine semen tube 01 is passed through the support rings 34. The servo motor 35 is then controlled by the control console 12 to drive the support block 33 to rotate 180° to reset. The support rings 34 will then drive the frozen bovine semen tube 01 to rotate out of the warm water space. The thin tube 01 is rotated into the warm water space to begin the thawing operation of the frozen bovine semen tube 01. The servo motor 35 continuously controls the support block 33 to swing back and forth. The support block 33 drives the frozen bovine semen tube 01 to swing back and forth in the warm water space through the support ring 34, thus eliminating the need for manual shaking. The swing time of the frozen bovine semen tube 01 can be precisely controlled according to the program of the control console 12. After thawing is completed, the servo motor 35 drives the support block 33 to rotate 180° again. At this time, the support ring 34 drives the frozen bovine semen tube 01 to rotate out of the warm water space, thus automatically completing the swing thawing operation of the frozen bovine semen tube 01. Then, the frozen bovine semen tube 01 is taken out and artificial insemination is performed on the cow to complete the breeding.

[0040] like Figure 3As shown, it also includes: a signal transmitter 36, a signal receiver 37, a gear 38, and an arc-shaped rack 39. The signal transmitter 36 is mounted on the rotating shaft 31, and the signal receiver 37, located directly below the signal transmitter 36, is mounted on the defrosting frame 3. The signal receiver 37 is adapted to receive signals emitted from the signal transmitter 36 and is electrically connected to the control console 12. The gear 38 is connected to the front end of the rotating shaft 31, and the arc-shaped rack 39, which meshes with the gear 38, is connected to the hinge of the lid 2. When the lid 2 is opened and the frozen fine tube 01 is taken out of the defrosting frame 3, the lid 2 will drive the arc-shaped rack 39 to rotate and mesh with the gear 38. The gear 38 will drive the insulation frame 32 and the signal transmitter 36 to rotate 180° through the rotating shaft 31. The insulation frame 32 will drive the support ring 34 on the support block 33 to rotate 180°. When the frozen beef fine tube 01 is taken out for thawing, the support ring 34 is automatically and synchronously rotated out of the warm water space, making it convenient to place the frozen beef fine tube 01 on the support ring 34. Then, when the box lid 2 is closed, it will drive the arc rack 39 to rotate in the opposite direction and mesh with the gear 38. The gear 38 will drive the insulation frame 32 and the signal transmitter 36 to rotate 180° in the opposite direction through the rotating shaft 31. At this time, the support ring 34 will drive the frozen beef fine tube 01 to rotate into the warm water space, and the signal transmitter 36 will correspond to the signal receiver 37 and transmit a signal to the signal receiver 37. The signal receiver 37 will control the servo motor 35 through the control console 12 to drive the support ring 34 to drive the frozen beef fine tube 01 to swing back and forth in the warm water space. Thus, after the frozen beef fine tube 01 is taken out and placed and the box lid 2 is closed, the swing thawing operation of the frozen beef fine tube 01 is automatically started.

[0041] like Figures 5-9 As shown, it also includes: a support frame 41 and a tube rack 42. There are two lids 2, facing each other left and right. The freezer 1 has two support frames 41 facing each other left and right, corresponding to the two lids 2 respectively. Each support frame 41 is equipped with a tube rack 42. The tube rack 42 has a first support rod 43 and a second support rod 44. The upper part of the first support rod 43 of the two tube racks 42 is respectively provided with openings suitable for accommodating 0.25mm beef jelly tubes 01 and 0.5mm beef jelly tubes 0. The upper part of the support rod 44 in the two thin tube racks 42 has slots 1 and 2 respectively, which are suitable for accommodating 0.25mm and 0.5mm beef jelly tubes 01. The 0.25mm and 0.5mm beef jelly tubes 01 are placed on the support rods 43 and 44 on the two thin tube racks 42, respectively, so as to facilitate differentiation and diversify the use. In addition, the two lids 2 can be opened and closed separately as needed to prevent excessive leakage of cold air in the freezer 1.

[0042] like Figure 10 and Figure 12As shown, it also includes: insulated windows 11, with two insulated windows 11 hinged to the freezer 1 facing each other, each insulated window 11 corresponding to two fine tube racks 42. The fine tube racks 42 are slidably placed on the upper side of the support frame 41. By opening the insulated windows 11 and moving the fine tube racks 42 forward, they can be taken out from the freezer 1, thus facilitating the placement of frozen bovine fine tubes 01. The upper side of the fine tube racks 42 has slots suitable for placing other fine tube racks 42 on the lower side, so that the fine tube racks 42 can be stacked, facilitating the initial pre-cooling and programmed freezing operations of multiple fine tube racks 42 and multiple layers of frozen bovine fine tubes 01. Specifically, when the frozen bovine fine tubes 01 are used for breeding, a programmed freezing machine or other machine is needed to perform initial pre-cooling and freezing operations on the freshly filled frozen bovine fine tubes 01, and then place them in different containers for freezing and insulation, so that the frozen bovine fine tubes 01 are made in the same work room and transferred to different places for breeding.

[0043] like Figure 5 , Figure 8 and Figure 11 As shown, it also includes: a slide rod 51, a clamping plate 52, a compression spring 53, and a top rod 54. The support frame 41 is slidably disposed inside the freezer 1. Moving the support frame 41 upwards allows for easy removal of the frozen bovine fine tubes 01 from the fine tube rack 42. A slide rod 51 is slidably disposed on one side of the fine tube rack 42. The first end of the slide rod 51 is connected to a clamping plate 52 that slidably engages with the fine tube rack 42. The clamping plate 52 is suitable for stably clamping the frozen bovine fine tubes 01 placed on the support rod 43 and the support rod 44 onto the fine tube rack 42 to improve stability during transfer and transportation and prevent damage from collisions to the frozen bovine fine tubes. A compression spring 53 is connected between the clamping plate 52 and the fine tube rack 42 and surrounds the slide rod 51. The compression spring 53 is used to reset the clamping plate 52 after sliding. The second end is wedge-shaped. The freezer 1 is equipped with a top rod 54 suitable for squeezing the second end of the slide rod 51. When the thin tube rack 42 is placed on the support frame 41 inside the freezer 1 through the open insulated window 11, the top rod 54 will squeeze the slide rod 51. The slide rod 51 will drive the clamping plate 52 to move and clamp the frozen beef fine tube 01 onto the thin tube rack 42. This can automatically align the frozen beef fine tube 01 and stabilize the frozen beef fine tube 01. When the frozen beef fine tube 01 is taken out, the upward movement of the support frame 41 will drive the thin tube rack 42 to move upward. In this way, the slide rod 51 will disengage from the top rod 54, and the clamping plate 52 will automatically reset and release the frozen beef fine tube 01 under the action of the compression spring 53. The same applies when the thin tube rack 42 is moved forward. The clamping plate 52 will reset and be in a loose state.

[0044] like Figures 12-14As shown, it also includes: a wedge-shaped limiting block 61, a connecting rod 62, a V-shaped lever 63, a torsion spring 64, and an L-shaped lever 65. A wedge-shaped limiting block 61 is slidably mounted on the support frame 41. A connecting spring (not shown in the figure, but easily understood and implemented by those skilled in the art; the connecting spring is only for the limiting block to move upwards and reset after downward movement) is connected to one side of the wedge-shaped limiting block 61 and slides on the support frame 41. A V-shaped lever 63 for actuating the connecting rod 62 is rotatably mounted inside the freezer 1. A torsion spring 64 is connected between the V-shaped lever 63 and the freezer 1, located inside the freezer 1. The insulated window door 1... The hinge of 1 is connected to an L-shaped lever 65 for actuating the V-shaped lever 63. When the insulated window door 11 is opened, the insulated window door 11 will drive the L-shaped lever 65 to rotate and actuate the V-shaped lever 63. The torsion spring 64 deforms, and the V-shaped lever 63 will rotate and actuate the connecting rod 62 to move downward. The connecting rod 62 will drive the wedge-shaped limiting block 61 to move downward. Thus, the thin tube frame 42 is automatically unlocked when the insulated window door 11 is opened. When the insulated window door 11 is closed, the L-shaped lever 65 will rotate in the opposite direction to release the V-shaped lever 63. The V-shaped lever 63 will rotate in the opposite direction to release the connecting rod 62 under the action of the torsion spring 64. The wedge-shaped limiting block 61 will move upward under the action of the connecting spring to block the thin tube frame 42 on the support frame 41, thereby achieving self-locking.

[0045] like Figure 5 and Figure 15 As shown, it also includes: a support plate 71, a pressure rod 72, and a connecting rod 73. The second slot on the second support rod 44 is L-shaped. The upper part of the freezer 1 is connected to the support plate 71, and the lower part of the support plate 71 is connected to the pressure rod 72 located above the second support rod 44. The pressure rod 72 is adapted to press down to lift the frozen beef fine tube 01 from the second slot of the second support rod 44. A connecting rod 73 is rotatably provided between the lid 2 and the support frame 41. When the lid 2 is opened, the lid 2 will drive the support frame 41 to move upward through the connecting rod 73. The upward movement of the support frame 41 will drive the fine tube rack 42 and the frozen beef fine tube 01 on the fine tube rack 42 to move upward. In this way, when it is necessary to take out the frozen beef fine tube 01, the frozen beef fine tube 01 will automatically move upward and closer. During the upward movement, the frozen beef fine tube 01 will be blocked by the pressure rod 72. At this time, the frozen beef fine tube 01 will be lifted from the second slot of the second support rod 44 (as shown). Figure 15 As shown in the diagram, the steps of opening the lid 2, moving the tube rack 42 upward, loosening the clamp 52, and raising the frozen fine tube 01 are interconnected and work together to facilitate the removal of the frozen fine tube 01. Furthermore, the highest point of the frozen fine tube 01 is lower than the opening above the freezer 1, so that the removal of the frozen fine tube 01 is only carried out inside the freezer 1, ensuring the temperature of the remaining frozen fine tubes 01.

[0046] like Figure 5 and Figure 15 As shown, it also includes: support plate 2 74 and limiting frame 75. Two support plates 2 74 are connected to the freezer 1, and the limiting frame 75 is connected to the support plate 2 74. The limiting frame 75 has multiple through slots for the frozen beef jelly tube 01 to pass through after it is tilted up. After the frozen beef jelly tube 01 is tilted up, the through slots on the limiting frame 75 can provide front-back direction limiting for the frozen beef jelly tube 01, ensuring the stable operation of taking out the frozen beef jelly tube 01.

[0047] like Figure 5 and Figure 15 As shown, it also includes: a baffle plate 81 and a connecting rod 82. A support plate 74 blocks the top of the support rod 44. The baffle plate 81 is rotatably installed inside the freezer 1. A connecting rod 82 is rotatably installed between the support frame 41 and the baffle plate 81. The baffle plate 81 has an unfolded state and a retracted state as the support frame 41 moves up and down. In the unfolded state, the baffle plate 81 blocks the bottom of the support rod 43. When the lid 2 is opened and the frozen beef jelly tube 01 needs to be taken out, the support frame 41 moves up and will drive the baffle plate 81 to swing to the unfolded state through the connecting rod 82. At this time, the baffle plate 81, together with the support plate 74, blocks the two sides of the frozen beef jelly tube 01 that are raised on the support rod 44 (e.g., Figure 15 (As shown) and a curved flow channel is formed along the frozen fine tube 01, which can reduce the loss of cold air in the freezer 1 and allow the cold air to be discharged close to the raised frozen fine tube 01, thereby using the cold air to maintain the freezing temperature of the frozen fine tube 01.

[0048] In a preferred embodiment, during the mating process, once the frozen bovine sperm tube 01 has thawed, the end of the frozen bovine sperm tube 01 is usually cut open manually using tools such as scissors. However, carrying and handling other tools such as scissors would cause additional trouble for the mating operation. Therefore, this embodiment also installs a special opening device for the frozen bovine sperm tube 01 on both the left and right sides of the upper rear part of the freezing box 1 to facilitate the opening operation of the frozen bovine sperm tube 01 without the need for additional tools.

[0049] A method for inseminating bovine frozen semen based on the above embodiments includes: first, selecting highly motile and morphologically normal sperm, diluting them, and then filling them into 0.25mm bovine frozen semen tubes 01. During filling, only 0.22mm is filled, leaving 0.03mm of air in the 0.25mm bovine frozen semen tube 01 to ensure space for thermal expansion and contraction and prevent tube bursting. If 0.5mm bovine frozen semen tubes 01 are used, only 0.44mm is filled, leaving 0.06mm of air in the 0.5mm bovine frozen semen tube 01. The filling method can be adjusted according to the actual situation to avoid the bursting of the frozen semen tube 01 due to thermal expansion and contraction. Then, the frozen semen tube 01 is placed in a freezer box 1 at a temperature of -196°C for freezing. When in use, the frozen semen tube 01 is taken out of the freezer box 1 and automatically thawed in the thawing box 3 by swinging. The servo motor 35 is used to provide power for the swinging of the frozen semen tube 01, so as to eliminate the need for manual swinging. After thawing, the sperm in the frozen semen tube 01 is used to artificially inseminate the cow to complete the breeding.

[0050] Obviously, the embodiments described above are only some embodiments of the present invention, and not all embodiments. They only express the preferred implementation of the present invention and are described in a relatively specific and detailed manner, but should not be construed as limiting the scope of the present invention.

[0051] It should be noted that, for those skilled in the art, various modifications, additions or subtractions, improvements and substitutions can be made without departing from the concept of the present invention. Therefore, based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

Claims

1. A thawing-assisted insemination device for bovine frozen semen, characterized in that, include: The freezer (1) is equipped with a control console (12), a lid (2) and a defrosting frame (3). The control console (12) has a built-in controller. The lid (2) is hinged to the upper part of the freezer (1). The defrosting frame (3) is equipped with a rotating shaft (31). The end of the rotating shaft (31) is connected to a heat preservation frame (32). A warm water space is left between the heat preservation frame (32) and the defrosting frame (3). The warm water space has a closed state and an open state that change with the rotation of the heat preservation frame (32). A servo motor (35) is installed on the heat preservation frame (32). The control console (12) is electrically connected to the servo motor (35). A support block (33) is connected to the output shaft of the servo motor (35). A support ring (34) located in the warm water space is connected to one side of the support block (33). The support ring (34) is used to support the frozen bovine fine tube (01). It also includes: a signal transmitter (36) installed on the rotating shaft (31), a signal receiver (37) installed on the defrosting frame (3), the signal receiver (37) being adapted to receive the signal emitted from the signal transmitter (36), the signal receiver (37) being electrically connected to the control console (12), a gear (38) being connected to the front end of the rotating shaft (31), and an arc-shaped rack (39) meshing with the gear (38) being connected to the hinge of the cover (2). It also includes: a support frame (41) and a tube rack (42). There are two lids (2). The freezer (1) is provided with two support frames (41). The two support frames (41) correspond to the two lids (2) respectively. Each of the two support frames (41) is provided with a tube rack (42). The tube rack (42) has a support rod one (43) and a support rod two (44). The support rod one (43) of the two tube racks (42) has a slot one suitable for accommodating a 0.25mm frozen beef fine tube (01) and a 0.5mm frozen beef fine tube (01) respectively. The support rod two (44) of the two tube racks (42) has a slot two suitable for accommodating a 0.25mm frozen beef fine tube (01) and a 0.5mm frozen beef fine tube (01) respectively. It also includes: two insulated windows (11) are hinged on the freezer (1), the two insulated windows (11) correspond to two thin tube racks (42) respectively, the thin tube racks (42) are slidably placed on the upper side of the support frame (41), and the upper side of the thin tube racks (42) has a slot suitable for placing the lower side of the other thin tube racks (42); It also includes: a support frame (41) slidably disposed inside the freezer (1), a slide rod (51) slidably disposed on one side of the thin tube rack (42), the first end of the slide rod (51) is connected to a clamping plate (52) that slidably engages with the thin tube rack (42), a compression spring (53) is connected between the clamping plate (52) and the thin tube rack (42), the second end of the slide rod (51) is wedge-shaped, and a top rod (54) suitable for squeezing the second end of the slide rod (51) is provided inside the freezer (1); It also includes: a wedge-shaped limiting block (61) is slidably provided on the support frame (41), a connecting spring is connected between the wedge-shaped limiting block (61) and the support frame (41), a connecting rod (62) is connected to one side of the wedge-shaped limiting block (61), a V-shaped lever (63) for moving the connecting rod (62) is rotatably provided inside the freezer (1), a torsion spring (64) is connected between the V-shaped lever (63) and the freezer (1), and an L-shaped lever (65) for moving the V-shaped lever (63) is connected to the hinge of the heat-insulating window door (11). It also includes: the second slot on the second support rod (44) is L-shaped, the first support plate (71) is connected to the freezer (1), the lower part of the first support plate (71) is connected to the pressure rod (72) located above the second support rod (44), the pressure rod (72) is suitable for pressing down to lift the frozen fine tube (01) from the second slot on the second support rod (44), and a connecting rod (73) is rotatably provided between the lid (2) and the support frame (41). It also includes: two support plates (74) connected to the freezer (1), a limit frame (75) connected to the support plate (74), and the limit frame (75) having multiple through slots for the raised bovine frozen fine tube (01) to pass through.

2. The thawing-assisted insemination device for bovine frozen semen as described in claim 1, characterized in that, Also includes: The second support plate (74) blocks the top of the second support rod (44). A baffle plate (81) is rotatably provided inside the freezer (1). A connecting rod (82) is rotatably provided between the support frame (41) and the baffle plate (81). The baffle plate (81) has an unfolded state and a retracted state as the support frame (41) moves up and down. When the baffle plate (81) is unfolded, it blocks the bottom of the first support rod (43).

3. The insemination method using the thawing-assisted insemination device for bovine frozen semen insemination according to any one of claims 1-2, characterized in that, The process includes: S1) Selecting high-motility and normal-morphological sperm, diluting them, and filling them with 0.25mm frozen bovine sperm tubes (01). When filling, only 0.22mm and 0.03mm of air are left in the 0.25mm frozen bovine sperm tubes (01) to ensure space for thermal expansion and contraction and to avoid tube bursting; S2) The frozen bovine sperm tubes (01) are then placed in a freezer (1) at a temperature of -196°C for freezing; S3) When using the frozen bovine sperm tubes (01), they are taken out of the freezer (1) and automatically thawed in a swinging motion in the thawing frame (3). A servo motor (35) is used to provide power for the swinging motion of the frozen bovine sperm tubes (01); S4) After thawing, the sperm in the frozen bovine sperm tubes (01) is used to artificially inseminate the cow to complete the mating.

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