Molecular marker of bhmt gene related to the resistance to hepatopancreatic coccidiosis in penaeus vannamei and detection primer and application thereof

By screening for BHMT gene molecular markers associated with resistance to shrimp hepatocellular carcinoma, and using SNP marker detection primers for genotyping, the problem of low breeding efficiency in existing technologies has been solved, achieving rapid and accurate breeding results.

CN120384137BActive Publication Date: 2026-06-30NINGBO UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NINGBO UNIV
Filing Date
2025-05-19
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Existing technologies make it difficult to efficiently and accurately breed new Litopenaeus vannamei varieties with stable resistance to enterocytozoonosis through molecular marker-assisted breeding methods, resulting in long breeding cycles and low efficiency.

Method used

We screened for BHMT gene molecular markers associated with resistance to shrimp hepatocystis disease, used SNP marker detection primers for genotyping, and selected individuals with TA, CT, and GG genotypes as reserve parents for breeding.

Benefits of technology

This method enables rapid and accurate screening of individuals resistant to shrimp hepatocellular carcinoma, improving breeding efficiency and accuracy, ensuring the genotypic stability of selected individuals, and providing a sound breeding foundation.

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Abstract

The application discloses a BHMT gene molecular marker related to Litopenaeus vannamei resistance to hepatopancreatic intestinal coccidiosis and a detection primer and application thereof, and has the characteristics that the molecular marker comprises three SNP sites on the BHMT gene; the nucleotide sequence of the molecular marker A is shown in SEQ ID NO. 1, the mutation type at the 252th base is T>A, the nucleotide sequence of the molecular marker B is shown in SEQ ID NO. 2, the mutation type at the 93th base is C>T, and the nucleotide sequence of the molecular marker C is shown in SEQ ID NO. 2, and the mutation type at the 636th base is G>A; the parent shrimp with the TA genotype of the BHMT-8159 site, the CT genotype of the BHMT-12167 site and the GG genotype of the BHMT-12710 site is selected as the parent shrimp resistant to hepatopancreatic intestinal coccidiosis; and the application has the advantages of good breeding efficiency and accuracy.
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