Thermostable subtilisin variants and uses thereof
By introducing specific amino acid mutations into B. subtilisin BPN’, peptide-stabilized ligase is formed, the stability of existing enzymes under external stress factors is solved, the thermal stability and catalytic efficiency of the enzyme are improved, and it is suitable for industrial peptide synthesis.
Patent Information
- Application Number
- CN202380087739.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2023-01-25
- Filing Date
- 2023-12-19
- Publication Date
- 2025-07-29
AI Technical Summary
Existing peptide ligases are not stable enough during external stress factors such as cosolvents, pH changes, freezing and heating, resulting in loss of activity, limiting the efficiency and yield of enzymatic synthetic peptides, especially in industrial applications, requiring higher enzyme stability and solubility.
By introducing specific amino acid mutations on the basis of subtilisin BPN', peptide-stabilized ligases are formed, including amino acid deletion and replacement, such as S221C, P225N, and a combination mutation selected from H17W, S18K, N25G, V30I, S63G, S204N, E251L, N43K and S87D, the thermal stability of the enzyme and tolerance to cosolvents are improved.
It enhances the thermal stability of the enzyme and its tolerance to cosolvents, improves the melting temperature and catalytic activity of the enzyme, reduces the loss of activity caused by unfolding and denaturation, and improves the stability and catalytic efficiency of the enzyme.