Collagen peptide as well as preparation method and application thereof

Through the dual-system collaborative expression and low-temperature enzyme cutting methods, the problem of high-yield and high-stability collagen peptides in cosmetics and health foods is solved, and the efficient production and application of high-purity collagen peptides are achieved to meet market demand.

CN120399094APending Publication Date: 2025-08-01JIANGSU RUNJIE BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202510627535.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-15
Publication Date
2025-08-01

AI Technical Summary

Technical Problem

The prior art is difficult to meet the needs of recombinant collagen peptides with high yield, high stability and non-sensitivity in cosmetics and health foods at the same time. Traditional processes lead to loss of activity or degradation, and a single expression system cannot balance expression efficiency and product integrity.

Method used

Using the method of dual-system collaborative expression and cryogenic enzyme cleavage, the collagen peptide was expressed in E. coli and Pichia cerevisiae, combined with the N-GST tag, HRV-3C enzyme cleavage site and a-factor signal peptide, and the efficient production of collagen peptide was achieved in stages.

Benefits of technology

The expression and purity of collagen peptides have been significantly improved, the degradation rate is reduced by 80%, the moisturizing property is improved by 35%, and the cell proliferation rate is increased by 55%, meeting the high standards of cosmetics and health foods.

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Abstract

The invention discloses a novel collagen peptide as well as a preparation method and application thereof. The collagen peptide contains a specific amino acid sequence and has an N-GST tag, an HRV-3C protease cleavage site and a collagen III structural domain, the molecular weight of the collagen peptide is 15.3 kDa to 41.7 kDa, and the isoelectric point (pI) of the collagen peptide is 6.13 kDa to 9.13 kDa. According to the preparation method, by optimizing an escherichia coli and pichia pastoris double-expression system and combining staged induction, fusion tag synergistic purification and low-temperature enzyme digestion processes, the expression efficiency is remarkably improved, and protein degradation is reduced. The collagen peptide has excellent moisture retention in cosmetics and can promote cell proliferation in health food, the preparation method is creative, and the technical problems that in the prior art, the heterologous expression efficiency is low, and products are easy to degrade are solved.
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