Monoclonal antibody combination for detecting cat NT-proBNP protein and application thereof

By developing monoclonal antibodies combinations 3A6 and 5H5, a dual-antibody sandwich ELISA detection system was constructed, which solved the problems of poor antibody specificity and low sensitivity in the diagnosis of cat cardiomyopathy, and achieved efficient and specific recognition of cat NT-proBNP protein, supporting early screening and accurate diagnosis of cat cardiomyopathy.

CN120427916AActive Publication Date: 2025-08-05BEIJING SUBENYUANHE BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202510855251.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-06-25
Publication Date
2025-08-05
Estimated Expiration
2045-06-25

AI Technical Summary

Technical Problem

The lack of high specificity and high sensitivity monoclonal antibodies in the existing detection methods makes it difficult to achieve accurate diagnosis of cat cardiomyopathy. The existing detection methods have problems with strong cross-reaction and insufficient sensitivity.

Method used

A monoclonal antibody combination, including monoclonal antibodies 3A6 and 5H5, was developed to specifically recognize cat NT-proBNP protein, and to improve detection sensitivity and specificity by constructing a dual-antibody sandwich ELISA detection system.

Benefits of technology

It realizes efficient and specific recognition of cat NT-proBNP protein, with detection sensitivity of 100 pg/mL, reducing cross-reactions, and provides a stable and highly repeatable diagnostic tool to support early screening and accurate diagnosis of cat cardiomyopathy.

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Abstract

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting cat NT-proBNP protein and application of the monoclonal antibody combination. The monoclonal antibody combination provided by the invention comprises a monoclonal antibody 3A6 and a monoclonal antibody 5H5. The two antibodies respectively have unique heavy chain and light chain variable region complementarity determining regions, and the amino acid sequences of the two antibodies are defined in detail (SEQ ID NO.1-12). The antibody combination can specifically recognize and efficiently bind the cat NT-proBNP protein, and is suitable for developing a high-sensitivity and high-specificity diagnostic tool for cat cardiomyopathy. By constructing a double-antibody sandwich ELISA detection system, the lowest detection limit can reach 100 pg / mL, and good specificity and stability are shown.
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