A weathered marble waste stone powder resource utilization composition and application thereof

Abstract

The application discloses a kind of weathered marble waste stone powder resource utilization composition and application thereof, belong to environmental protection technical field.The weathered marble waste stone powder resource utilization composition includes marble waste stone powder, soil and microalgae biological fertilizer SynMiCom.The application mixes soil in marble waste stone powder and then adds SynMiCom, which can significantly reduce the pH of marble waste stone powder, significantly increase the content of reduced nitrogen in marble waste stone powder, significantly increase the content of available phosphorus in marble waste stone powder, significantly increase the enzyme activity in marble waste stone powder, and significantly promote the growth of peppers in marble waste stone powder.The application utilizes the long-term weathered marble waste stone powder, which has low cost and high application value.

Description

Technical Field

[0001] This invention relates to the field of stone waste resource utilization, specifically to a resource utilization composition for weathered marble waste powder and its application. Background Technology

[0002] Marble waste powder is a powdery waste generated during the processing of natural stone, accounting for approximately 10.00% to 35.00% of the total stone volume.

[0003] Currently, research on the utilization of marble waste powder mainly focuses on the recovery of valuable elements, building materials, ceramic materials, and soil conditioners. In soil conditioners, waste powder is primarily used to improve acidic and saline soils; however, this method cannot solve the problem of large-scale waste powder stockpiling, and different types of waste powder have different compositions, making them potentially unsuitable as soil conditioners.

[0004] For example, Chinese patent application number 202211496972.5 discloses a method for preparing an acidic soil conditioner based on mine waste stone powder, its product, and its application. This method involves uniformly mixing waste stone powder, biochar, waste seashells, livestock manure, potassium humate, and growth hormones to obtain the acidic soil conditioner. The acidic soil conditioner provided by this technical solution has a relatively complex composition and processing procedure, making it unsuitable for large-scale stockpiling of weathered waste stone powder.

[0005] Chinese patent application number 201811072245.X discloses a saline soil conditioner, the components of which include ionic rare earth slag, activated sludge particles, kaolin, modified waste rock powder, cement, and quicklime. This technology involves not only complex raw materials but may also cause secondary damage to the soil environment.

[0006] Chinese patent application number 202311611213.3 discloses a soil conditioner and its preparation method. This technology involves the resource utilization of palygorskite powder. However, the utilization process requires grinding the palygorskite powder into granules, which increases costs. This production process is not suitable for waste stone powder that has been weathered for many years. Summary of the Invention

[0007] The purpose of this invention is to address the shortcomings of existing waste stone powder utilization technologies by proposing a resource utilization composition for weathered marble waste stone powder and providing specific applications of this composition.

[0008] A composition for the resource utilization of weathered marble waste powder, comprising marble waste powder, soil, and synthetic microalgae community (SynMiCom).

[0009] Furthermore, the microalgae biofertilizer SynMiCom comprises a mixture of three microalgae: Chroococcus sp., Graesiella emersonii, and Chlamydomonas sp.; the concentration of each of the three microalgae is 5 × 10⁻⁶. 6 The cells / mL are mixed in a volume ratio of 1 to 5:1 to 5:1 to 5, preferably 1:1:1.

[0010] Furthermore, the mass ratio of the marble waste powder to the soil is 5:4-6.

[0011] Furthermore, the amount of microalgae bio-fertilizer added is: 0.1-0.8 mL / g of microalgae bio-fertilizer / marble waste stone powder and soil mixture.

[0012] Furthermore, the soil selected is the topsoil of the marble waste powder dump site or its surrounding area.

[0013] Application of a resource utilization composition for weathered marble waste powder in the resource utilization of marble waste powder.

[0014] Based on the above composition, a method for the resource utilization of weathered marble waste powder is to mix soil and microalgae bio-fertilizer SynMiCom into the marble waste powder to improve the physicochemical properties of the weathered marble waste powder and then use it for crop cultivation.

[0015] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows:

[0016] This invention involves mixing soil with marble waste powder and then adding SynMiCom, which significantly reduces the pH of the marble waste powder, significantly increases the content of degraded nitrogen, significantly increases the content of available phosphorus, significantly increases the enzyme activity, and significantly promotes the growth of chili peppers in the marble waste powder. This invention utilizes long-term weathered marble waste powder as a resource, with low cost and high application value. Attached Figure Description

[0017] Figure 1 The pH of marble waste powder after 40 days of improvement was determined for each treatment in Case 1.

[0018] Figure 2 To implement Case 1, the organic matter content of marble waste powder was measured 40 days after improvement.

[0019] Figure 3 To implement the reduction of nitrogen content in marble waste powder after 40 days of improvement for each treatment in Case 1.

[0020] Figure 4 The available phosphorus content in marble waste powder was measured 40 days after the treatments in Case 1 were improved.

[0021] Figure 5 To implement Case 1, the urease activity of marble waste powder was measured 40 days after modification.

[0022] Figure 6 To implement Case 1, the sucrase activity of marble waste powder was measured 40 days after modification.

[0023] Figure 7 To measure the peroxidase activity of marble waste powder after 40 days of improvement in each treatment of Case 1.

[0024] Figure 8 To implement Case 1, the catalase activity of marble waste powder was measured 40 days after modification.

[0025] Figure 9 To implement Case 2, process the chili pepper photos.

[0026] Figure 10 The chili plant height was measured 40 days after the treatments in Case 2 were implemented.

[0027] Figure 11 The chili stem diameter was measured 40 days after the improvement of each treatment in Case 2.

[0028] Figure 12 SPAD was applied to chili leaves 40 days after the treatments in Case 2 were modified.

[0029] Figure 13 The actual photosynthetic efficiency of chili leaves after 40 days of improvement was measured for each treatment in Case 2.

[0030] Figure 14 To implement the root vigor of chili peppers in each treatment of Case 2 40 days after improvement.

[0031] Figure 15 The root length of chili peppers after 40 days of improvement was measured for each treatment in Case 2.

[0032] Figure 16 The number of lateral roots of chili peppers after 40 days of improvement for each treatment in Case 2.

[0033] Figure 17 The fresh weight of the chili peppers on the field 40 days after the treatments in Case 2 were measured.

[0034] Figure 18 The underground fresh weight of chili peppers after 40 days of improvement was used to implement the treatments in Case 2.

[0035] Among them, WSP is the control group, which is the marble waste powder treatment group after weathering; WSP+S is the marble waste powder treatment group after soil mixing; WSP+SynMiCom is the marble waste powder treatment group after weathering with added microalgae bio-fertilizer; and WSP+S+SynMiCom is the marble waste powder treatment group after soil mixing with added microalgae bio-fertilizer. Detailed Implementation

[0036] The present invention will be further described below with reference to specific embodiments and accompanying drawings.

[0037] Example 1: Mix soil into flowerpots containing marble waste powder and apply SynMiCom periodically.

[0038] (1) Marble waste powder packaging: 250g±10g of soil is mixed into the weathered marble waste powder, and then packaged into flower pots, 500g±10g per pot; the physical and chemical properties of the soil are pH=6.5, organic matter content, alkaline nitrogen content and available phosphorus content are 12g / kg, 21mg / kg and 31mg / kg respectively; the mass of the soil added is 80wt% to 120wt% of the mass of the marble waste powder, and the mass of the soil used in this embodiment is 50wt% of the mixture;

[0039] (2) SynMiCom preparation: *Chroococcus* sp., *Graesiella emersonii*, and *Chlamydomonas* sp. were purchased from the Freshwater Algae Culture Collection of the Chinese Academy of Sciences. The three microalgae were cultured to the logarithmic growth phase using BG11 medium. The culture solutions of the three microalgae in the logarithmic growth phase were centrifuged at 5000 r / min for 3 min, and then brought to the original volume with sterile distilled water. The mixture was then centrifuged again at 5000 r / min for 3 min. After washing twice, the concentration of each microalgae was adjusted to 5 × 10⁻⁶ with sterile distilled water. 6 SynMiCom was prepared by mixing cells / mL at a volume ratio of 1:1:1.

[0040] (4) Use SynMiCom once on the first day after the marble waste stone powder is packaged, and use 20mL per pot. Then use it once every 7 days for a total of 4 times. The amount of microalgae bio-fertilizer added is 0.1-0.8mL / g of the marble waste stone powder and soil mixture. In this case, the amount of microalgae bio-fertilizer used is 0.16mL / g.

[0041] (5) 40 days after fertilization, soil physicochemical indicators were measured, including pH, organic matter content, alkaline nitrogen content, available phosphorus content, urease activity, sucrase activity, peroxidase activity and catalase activity of marble waste powder.

[0042] Results analysis:

[0043] like Figure 1 As shown, compared to the WSP group, neither mixing with soil nor using SynMiCom alone significantly altered the pH of marble waste powder, but the combined use of mixing with soil and SynMiCom significantly reduced the pH of marble waste powder by 2.56%. Figure 2-8 As shown, compared with the WSP group, the mixed soil significantly increased the organic matter content, available nitrogen content, available phosphorus content, urease activity, sucrase activity, peroxidase activity, and catalase activity in marble waste powder by 212.51%, 65.16%, 194.06%, 57.60%, 44.05%, 73.49%, and 95.31%, respectively. Compared with the WSP group, the use of SynMiCom alone significantly increased the organic matter content, available nitrogen content, available phosphorus content, urease activity, sucrase activity, peroxidase activity, and catalase activity in marble waste powder by 114.56%, 104.46%, 122.02%, 29.48%, 30.95%, 51.59%, and 62.82%, respectively. The combined use of mixed soil and SynMiCom significantly increased the organic matter content, alkali-hydrolyzable nitrogen content, available phosphorus content, urease activity, sucrase activity, peroxidase activity, and catalase activity of marble waste powder by 184.41%, 216.58%, 239.33%, 66.55%, 61.90%, 98.56%, and 100.29%, respectively, compared to the WSP group.

[0044] In this implementation case 2, chili seedlings were planted in modified marble waste powder and SynMiCom was applied.

[0045] (1) The packaging of marble waste stone powder and the preparation of SynMiCom are the same as (1) and (2) in this implementation case 1.

[0046] (2) Preparation of base fertilizer: Urea: 7.43 g / L; Dipotassium hydrogen phosphate: 2.16 g / L; Potassium sulfate: 3.87 g / L. Apply once every 7 days, with a dosage of 20 mL each time.

[0047] (3) Seedling raising: Chili seeds were stirred in deionized water at 55℃ for 15 minutes and soaked at 25℃ for 8 hours. Subsequently, they were surface-sterilized by gently shaking in a 5% v / v bleach solution for 2 minutes, and then rinsed 10 times with deionized water to remove all bleach. The treated seeds were placed on gauze soaked in deionized water and placed in an artificial climate chamber at 28℃ in darkness for 3 days. Once the seeds showed signs of sprouting, they were sown in 12-cell seedling trays. The seedling trays were placed in an incubator (GCL–350, Jiangsu) with a light cycle of 16h / 8h (day / night) and a temperature of 28±2℃ / 18±2℃ (day / night). Seedlings were transplanted when they reached the three-leaf stage.

[0048] (4) 20 ml of lymMiCom was added to the chili seedlings on days 0, 7, 14, 21 and 28 after transplanting. The control group was given the same amount of sterilized water.

[0049] (5) 40 days after fertilization, measure the relevant indicators of the plant, including plant height, stem diameter, SPAD of leaves, actual photosynthetic efficiency, root length, root activity, number of lateral roots, above-ground fresh weight and underground fresh weight.

[0050] Results analysis:

[0051] like Figure 9 As shown, the chili pepper plants grown in marble waste powder were the shortest, and both soil mixing and the use of SynMiCom alone significantly promoted chili pepper growth. Figure 10-11 As shown, compared with the WSP group, soil mixing significantly increased the plant height and stem diameter of peppers by 19.64% and 11.64%, respectively. Further addition of SynMiCom significantly increased the plant height and stem diameter by 48.73% and 19.13%, respectively. Figure 12-18 As shown, soil mixing significantly increased the SPAD, actual photosynthetic efficiency, root length, root activity, number of lateral roots, aboveground fresh weight, and underground fresh weight of pepper leaves by 1.43%, 88.82%, 39.29%, 18.92%, 27.42%, 83.67%, and 54.05%, respectively, compared to the WSP group. Compared to the WSP group, using SynMiCom alone significantly increased the SPAD, actual photosynthetic efficiency, root length, root activity, number of lateral roots, aboveground fresh weight, and underground fresh weight of pepper leaves by 16.83%, 73.95%, 53.57%, 13.29%, 51.61%, 104.04%, and 160.35%, respectively, compared to the WSP group. The combined use of soil mixing and SynMiCom significantly increased the SPAD, actual photosynthetic efficiency, root length, root activity, number of lateral roots, aboveground fresh weight, and underground fresh weight of pepper leaves by 29.00%, 161.58%, 71.43%, 30.63%, 103.23%, 301.98%, and 344.03%, respectively, compared to the WSP group.

[0052] Conclusion: Both soil mixing and SynMiCom can promote the growth of chili peppers in marble waste powder, but the combination of the two has the best effect on the physicochemical properties of marble waste powder and the growth-promoting effect on chili peppers. Therefore, the marble waste powder, soil, and SynMiCom provided by this invention have a good soil improvement effect, promote the growth of economic crops such as chili peppers in marble waste powder, and ultimately realize the resource utilization of marble waste powder.

[0053] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of the present invention. It should be understood that the above descriptions are merely specific embodiments of the present invention and are not intended to limit the scope of the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A composition for the resource utilization of weathered marble waste powder, characterized in that, The composition comprises marble waste powder, soil, and SynMiCom microalgae biofertilizer; the SynMiCom microalgae biofertilizer comprises Chlorella vulgaris. Chroococcus sp., Emerson Lipoglucosium Graesiella emersonii wa-yakini Chlamydomonas sp. a mixture of the three; Chromosphereum Chroococcus sp., Emerson Lipoglucosium Graesiella emersonii wa-yakini Chlamydomonas The concentrations of the three microalgae were all 5 × 10⁻⁶. 6 The cells / mL and the three microalgae are mixed in a volume ratio of 1~5 : 1~5 : 1~5; the amount of the microalgae bio-fertilizer SynMiCom added is: microalgae bio-fertilizer SynMiCom : marble waste stone powder and soil mixture is 0.1-0.8ml : 1g.

2. The composition according to claim 1, characterized in that, The mass ratio of the marble waste powder to the soil is 5:4-6.

3. The composition according to claim 1, characterized in that, The soil selected is the topsoil from or around a marble waste powder dump site.

4. The application of the composition for resource utilization of weathered marble waste powder as described in claim 1 in the resource utilization of marble waste powder.

5. The application as described in claim 4, characterized in that, One method for the resource utilization of weathered marble waste powder is to mix soil and microalgae bio-fertilizer SynMiCom into the marble waste powder to improve its physical and chemical properties and then use it for crop cultivation.

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