Developmental-period optical intervention system for inducing shortsightedness susceptibility

Abstract

The invention discloses a developmental-period optical intervention system for inducing myopia susceptibility. A myopia susceptibility induction device of the system is used for inducing a myopia susceptibility state under purple light exposure, and a myopia susceptibility evaluation device is used for evaluating the myopia susceptibility state. The system can develop a myopia susceptibility model capable of accurately simulating myopia pathogenesis of children and adolescents, has high scientificity and rationality, can provide powerful support for an environment intervention mechanism in the early stage of myopia pathogenesis, and provides a theoretical basis for developing a new myopia prevention and treatment scheme.

Description

Technical Field

[0001] The present invention relates to an optical intervention system, in particular to a developmental optical intervention system for inducing myopia susceptibility. Background Art

[0002] Myopia in adolescents has become a global public health issue, with its prevalence increasing, particularly in East Asia. Myopia not only affects adolescents' visual health but can also lead to a range of complications, such as macular degeneration and glaucoma. Despite recent progress in the prevention and treatment of myopia, its pathogenesis remains largely unresolved. Current research focuses on the development of animal models, the etiology, pathogenesis, pathophysiological changes, and treatment approaches for myopia.

[0003] In animal model research, commonly used myopia models include form deprivation models, defocus models, and drug-induced models. Most of these models directly induce the occurrence of myopia. Although they can simulate the formation process of myopia, they have limitations in studying the early susceptibility factors of myopia. For example, the form deprivation model induces myopia through means such as eyelid suturing or covering, but this method is prone to infection, corneal deformation and other problems, and it is difficult to simulate the susceptible state before myopia occurs. In addition, existing myopia models focus more on the direct causes of myopia, while ignoring the changes in susceptibility under the interaction of genes and the environment. Therefore, there is an urgent need to construct a myopia susceptibility model to fill the gaps in existing research. Summary of the Invention

[0004] In order to solve the problems existing in the background technology, the present invention provides a developmental optical intervention system for inducing myopia susceptibility. The present invention can accurately simulate the animal model of the early susceptibility mechanism of adolescent myopia, which can not only provide a reliable tool for the intervention and prevention and control evaluation of myopia, but also provide an experimental basis for studying the early pathogenesis of myopia. The myopia susceptibility model of the present invention forms myopia susceptibility by simulating functional abnormalities caused by genetic abnormalities (such as abnormal light environment intervention), and then combines form deprivation to induce myopia. Compared with the traditional model of directly inducing myopia, the present invention can observe the risk factors and susceptibility changes before the occurrence of myopia at an earlier stage, provide a more powerful tool for studying the early pathogenesis of myopia, and can observe the risk factors and susceptibility changes before the occurrence of myopia at an earlier stage. In addition, the present invention has more advantages in pathophysiological theory and social status simulation, and can provide a theoretical basis for early intervention and prevention and control strategies for clinical myopia, and provide new ideas and experimental basis.

[0005] The technical solution adopted in the present invention is:

[0006] 1. A developmental optical intervention system for inducing myopia susceptibility, comprising:

[0007] A myopia susceptibility inducing device is used to induce a myopia susceptibility state under ultraviolet light exposure.

[0008] A myopia susceptibility assessment device is used to assess the myopia susceptibility state.

[0009] The myopia susceptibility inducing device comprises an ultraviolet light exposure box, in which a short-wavelength visible ultraviolet light source is provided for exposure for 12 hours a day and for 14 days.

[0010] The wavelength range of the short-wavelength visible violet light is 390-410 nm, and the illumination intensity is 20 lux.

[0011] The myopia susceptibility assessment device includes a full-spectrum white light exposure box, an opaque eye mask for form deprivation, a refractive index test device, and a computer, wherein the refractive index test device and the computer are electrically connected; the full-spectrum white light exposure box is provided with full-spectrum white light for 12 hours per day for 14 days; the opaque eye mask is worn on one of the eyes after exposure to violet light or full-spectrum white light; the refractive index test device performs refractive index tests on the eye after and without form deprivation exposure, and obtains the refractive index difference between the two eyes after exposure to violet light and full-spectrum white light respectively through a computer, and finally compares the refractive index difference between the two eyes after exposure to violet light and full-spectrum white light to assess myopia susceptibility.

[0012] During the refractive test, the eye wearing the opaque eyepatch serves as the deprivation eye, and the eye not wearing the opaque eyepatch serves as the non-deprivation eye. The opaque eyepatch is worn for 10 days. The refractive power difference between the deprivation eye and the non-deprivation eye after ultraviolet light exposure is 5.0 to 10.0 D. The refractive power difference between the deprivation eye and the non-deprivation eye after full-spectrum white light exposure is 0.0 to 5.0 D. If the refractive power difference is greater than 0.0 to 5.0 D, the patient is assessed as being susceptible to myopia.

[0013] 2. A method for constructing a mouse myopia susceptibility model, comprising:

[0014] The construction method constructs a mouse myopia susceptibility model by exposing mice to ultraviolet light; the specific steps of the construction method include:

[0015] The mice were placed in a violet light exposure box and continuously exposed to a short-wavelength visible violet light source with a wavelength range of 390-410 nm and a light intensity of 20 lux for 12 hours a day for 14 days, thereby constructing a mouse myopia susceptibility model.

[0016] The mice had just opened their eyes before the violet light exposure.

[0017] 3. A mouse myopia susceptibility model:

[0018] The mouse myopia susceptibility model was obtained by constructing a developmental optical intervention system that induces myopia susceptibility.

[0019] The present invention aims to overcome the shortcomings of existing animal myopia models and develop an animal model that can accurately simulate the pathogenesis of myopia in children and adolescents. The model is based on the pathogenesis of myopia in children and adolescents: that is, myopia susceptibility is formed under the influence of genes, and then the occurrence of myopia is eventually caused by environmental factors (such as long-term close-range eye use). The present invention proposes a method of inducing visual development abnormalities through abnormal light environment intervention (visible ultraviolet light exposure), thereby simulating functional abnormalities caused by genetic abnormalities and forming a myopia susceptibility model. On this basis, the occurrence of myopia is induced by form deprivation.

[0020] The violet light exposure myopia susceptibility model constructed by this invention involves exposure to short-wavelength visible violet light, starting after the animals open their eyes. This leads to abnormal neural development during the visual development stage, thereby establishing a myopia susceptibility model. This model is consistent with the currently accepted etiological view that the extensive use of artificial light sources increases the risk of myopia and better simulates the pathogenesis of myopia in humans.

[0021] From a pathophysiological perspective, the present invention's visible violet light-induced myopia susceptibility model in mice is highly scientific and rational. From the perspectives of social realities and disease onset prediction, this model can provide strong support for environmental intervention mechanisms in the early stages of myopia and offer a theoretical basis for developing new myopia prevention and treatment strategies. It's important to emphasize that the present invention constructs a myopia susceptibility model, not a direct model of myopia disease.

[0022] The beneficial effects of the present invention are:

[0023] The mouse myopia susceptibility model of the present invention induces visual developmental abnormalities through ultraviolet light exposure, mimicking functional abnormalities caused by genetic abnormalities to form myopia susceptibility. This model then combines form deprivation to induce myopia. This model can better simulate the pathogenesis of juvenile myopia, particularly the changes in susceptibility caused by the interaction between genes and the environment, providing a powerful tool for studying the early pathogenesis of myopia.

[0024] 2. The model presented in this paper can observe risk factors and susceptibility changes before the onset of myopia at an earlier stage, filling a gap in existing research. Compared with traditional models that directly induce myopia, the model presented in this paper can more accurately simulate the early stages of myopia, providing a theoretical basis for early intervention and prevention of myopia.

[0025] 3. The ultraviolet light exposure method used in this invention is simple to operate, low-cost, non-invasive, and has a stable model with high reproducibility. Ultraviolet light exposure can effectively induce visual development abnormalities and form myopia susceptibility, providing a good foundation for subsequent form deprivation to induce myopia.

[0026] 4. This model can be used not only to study the early pathogenesis of myopia but also to evaluate the effectiveness of myopia interventions. By combining ultraviolet light exposure with form deprivation, it can simulate the effects of various environmental factors on myopia susceptibility, providing an important theoretical basis for developing new myopia prevention and treatment strategies.

[0027] 5. The model construction method of the present invention has the advantages of low cost, simple operation, and non-invasiveness, making it suitable for application under various laboratory conditions. The successful construction of this model provides a good theoretical support and model reference for in-depth analysis of the early pathogenesis of myopia and the evaluation of new methods for intervention and prevention of myopia. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The following are the results of behavioral and refractive development tests of the control group (full spectrum white light) and experimental group (purple light) of mice on day 14 (P14). Figure 1 (A) is the weight statistics of the control group and experimental group of mice. Figure 1 (B) is a statistical graph of the immobility time in the forced swimming test of the control group and the experimental group of mice. Figure 1 (C) is a statistical diagram of the total movement distance of the control group and the experimental group of mice in the open field test. Figure 1 (D) is a statistical graph of the movement time in the middle area of the control group and the experimental group of mice in the open field test. Figure 1 (E) is the refractive power statistics of the control group and the experimental group of mice. Figure 1 (F) is the statistical diagram of the axial length of the control group and the experimental group of mice. Figure 1 (G) is a statistical diagram of the difference in binocular refractive power between the control group and the experimental group of mice after 10 days of right eye form deprivation. Figure 1 (H) is a statistical diagram of the difference in axial length growth between the control group and the experimental group of mice after 10 days of right eye form deprivation;

[0029] Figure 2 The following is a statistical chart of the refractive power and eye axis growth of mice at different times after exposure to full-spectrum white light and violet light and form deprivation on the 14th day (P14). Figure 2 (A) is a statistical diagram of the binocular refractive power difference of mice at day 14 (P14) after 1 week, 2 weeks and 3 weeks of exposure to full-spectrum white light and violet light. Figure 2 (B) is a statistical diagram of the binocular refractive power difference of mice on day 14 (P14) after being exposed to full-spectrum white light and purple light for 3 weeks and then undergoing 10 days of form deprivation. Figure 2 (C) is a statistical diagram of the difference in axial length growth of both eyes of mice after 3 weeks of exposure to full-spectrum white light and violet light and 10 days of form deprivation on day 14 (P14);

[0030] Figure 3 The following is the result of the evaluation of visual function development, refractive development and myopia susceptibility of mice exposed to full-spectrum white light and violet light for 2 weeks on day 21 (P21). Figure 3 (A) is light-adapted 3.0 cd / cm 2 Electroretinogram responses under the following conditions: Figure 3 (B) is dark-adapted 0.01 cd / cm 2 Electroretinogram responses under the following conditions: Figure 3 (C) is dark adaptation 3.0cd / cm 2 Electroretinogram responses under the following conditions: Figure 3 (D) is dark adaptation 3.0cd / cm 2 Electroretinogram oscillatory wave response diagram under conditions, Figure 3 (E) is a statistical diagram of the difference in binocular refractive power after form deprivation in mice. Figure 3 (F) is the statistical diagram of the difference in eye axis growth between the two eyes after form deprivation of mice. Figure 3 (G) is the statistical diagram of the light response amplitude of mouse cone cells. Figure 3 (H) is the statistical diagram of the light response amplitude of mouse cone bipolar cells. Figure 3 (I) is a statistical diagram of the light response amplitude of mouse rod cells, Figure 3 (J) is the statistical diagram of the light response amplitude of mouse rod bipolar cells. Figure 3 (K) is a statistical graph of the light response amplitude of the inner nuclear layer cells of mice. DETAILED DESCRIPTION

[0031] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0032] The developmental optical intervention system for inducing myopia susceptibility of the present invention includes a myopia susceptibility inducing device and a myopia susceptibility assessment device. The myopia susceptibility inducing device induces myopia susceptibility through ultraviolet light exposure. The myopia susceptibility inducing device includes an ultraviolet light exposure chamber equipped with a short-wavelength visible ultraviolet light source for 12 hours per day for 14 days. The short-wavelength visible ultraviolet light has a wavelength range of 390 to 410 nm and an illumination intensity of 20 lux. The myopia susceptibility assessment device assesses myopia susceptibility. The myopia susceptibility assessment device includes a full-spectrum white light exposure box, an opaque eye mask for form deprivation, and a refractive index examination device and a computer, and the refractive index examination device and the computer are electrically connected; the full-spectrum white light exposure box is provided with full-spectrum white light for 12 hours per day and for 14 days; the opaque eye mask is worn on one of the eyes after exposure to violet light or full-spectrum white light; the refractive index examination device performs refractive index examinations on the eye after and without form deprivation after exposure to violet light and full-spectrum white light, and obtains the refractive index difference between the two eyes after exposure to violet light and full-spectrum white light respectively through a computer, and finally compares the refractive index difference between the two eyes after exposure to violet light and full-spectrum white light to assess myopia susceptibility. The refractive index examination device specifically adopts a Photorefractor small animal infrared eccentric photographic ophthalmometer. During the refractive examination, the eye wearing the opaque eyepatch was used as the deprivation eye, and the eye not wearing the opaque eyepatch was used as the non-deprivation eye. The opaque eyepatch was worn for 10 days. The refractive power difference between the deprived eye and the non-deprivation eye after ultraviolet light exposure was 5.0 to 10.0 D. The refractive power difference between the deprived eye and the non-deprivation eye after full-spectrum white light exposure was 0.0 to 5.0 D. If the refractive power difference was greater than 0.0 to 5.0 D, the patient was assessed as susceptible to myopia.

[0033] The specific embodiments of the present invention are as follows:

[0034] First, adaptive feeding was performed: six clean-grade C57BL / 6 inbred mice weighing 5-7g were selected, including the mothers of each mouse. The physical health of all mice was checked before the experiment, and no special eye diseases were found. The breeding environment was quiet, with a constant room temperature of approximately 25°C. The mice had free access to food and water, and the mothers were given fresh green vegetables twice a day to supplement vitamin C. The experimental animals were given humane care according to the 3R principle (Replacement, Reduction, Refinement). The animals were adaptively fed for 4 days until their eyes opened on the 14th day (P14).

[0035] Then, ultraviolet light exposure was induced: After 4 days of adaptive feeding, the mice and their mothers were placed in a ultraviolet light exposure cage. The light source of the ultraviolet light exposure box was short-wavelength visible ultraviolet light with a wavelength range of 390-410nm and a light intensity of 20 lux. The exposure time was 12 hours per day, specifically from 7:00 to 19:00 every day, for 2 weeks, to obtain a mouse myopia susceptibility model as the experimental group. At the same time, full-spectrum white light was used as the control group. Ultraviolet light exposure can induce abnormal visual development in mice, simulate functional abnormalities caused by genetic abnormalities, and form myopia susceptibility.

[0036] Then, form deprivation was induced: After two weeks of violet light exposure, the mice were removed from the violet light exposure chamber and subjected to monocular form deprivation of the right eye using an opaque eye mask for 10 consecutive days. Form deprivation further induced myopia on top of the susceptibility induced by violet light exposure.

[0037] After modeling, the model was evaluated: After the violet light exposure and form deprivation, the mice were tested for refraction and anterior segment optical coherence tomography (OCT) measurements to assess the effectiveness of the myopia susceptibility model. The test results are as follows:

[0038] The experimental subjects were divided into two groups, the experimental group and the control group. The mice in the experimental group were exposed to visible violet light, while the mice in the control group were exposed to full-spectrum white light. After two weeks of exposure to the light environment, the mice were subjected to form deprivation of the right eye (10 days). The difference in binocular refractive power between the two groups was compared. The group with the larger difference was more susceptible to form deprivation myopia. Figure 1 As shown in (E), it can be seen that exposure to visible violet light for 2 weeks delayed the development of refractive power of mice in the experimental group, showing myopia. Figure 1 As shown in (F), it can be seen that 2 weeks of exposure to visible violet light delayed the development of the eye axis of the mice in the experimental group, which was manifested as a shorter eye axis. Figure 1 As shown in (G), it can be seen that the form deprivation of the experimental group induced more myopia after exposure to visible violet light, as shown in Figure 1 As shown in (H), it can be seen that after exposure to visible violet light, the form deprivation of the experimental group induced more axial length growth. It can be seen that the development of refractive power of the mice in the experimental group exposed to visible violet light was significantly delayed, showing more myopia. After form deprivation, the myopia induction in the experimental group exposed to visible violet light was significantly greater, showing a more obvious susceptibility to myopia; at the same time, the axial length of the mice in the experimental group exposed to visible violet light was significantly shorter, and after form deprivation, the axial length growth in the experimental group exposed to visible violet light was significantly greater, showing a more obvious susceptibility to myopia.

[0039] Further fundus examinations, retinal morphology testing, and related biomarker testing can be performed to more accurately evaluate the effectiveness of the myopia susceptibility model. Other testing methods include observing mouse behavior and weight changes, retinoscopy, ocular A-type ultrasound measurement, fundus photography and fluorescence angiography, hematoxylin-eosin staining (HE) and light microscopy to detect retinal morphology changes, serum inflammatory factor levels, and immunohistochemistry to detect the expression of related proteins in the retina.

[0040] The test results of mouse behavior and body weight changes are as follows:

[0041] 1) Weight changes: such as Figure 1 As shown in (A), 2 weeks after modeling, there was no significant difference in the body weight of the mice in the experimental group compared with the control group, indicating that 2 weeks of exposure to visible violet light did not affect the body weight of the mice.

[0042] 2) Forced swimming test: Figure 1 As shown in (B), 2 weeks after modeling, in the forced swimming test, there was no significant difference in the immobility time of the mice in the experimental group compared with the control group, indicating that 2 weeks of exposure to visible violet light did not affect the depressive-like behavior of mice.

[0043] 3) Open field test: Figure 1 (C) and Figure 1 As shown in (D), 2 weeks after modeling, in the open field test, there was no significant difference in the total distance moved and the time spent in the center of the open field in the experimental group compared with the control group. This shows that 2 weeks of exposure to visible violet light does not affect the anxiety-like behavior of mice.

[0044] All statistical data are expressed as mean ± standard error (mean ± SEM). Student's t-test or Mann-Whitney U test was used, and statistically significant differences were indicated when the probability value (p) was less than or equal to 0.05. In the figures, "*" indicates p value ≤ 0.05, "**" indicates p value less than 0.01, and "ns" indicates p value greater than 0.05, indicating no statistically significant difference.

[0045] Finally, the researchers conducted a mechanistic examination: After exposure to ultraviolet light, they examined the mice's visual function and retinal structure, revealing delayed visual development. The results indicate that the mice successfully exhibited typical myopia susceptibility characteristics, confirming the reliability and validity of the animal model.

[0046] After repeated experiments, the experimental groups all showed the same results. This shows that the mice in the experimental group successfully demonstrated typical myopia susceptibility characteristics, which meets the reliability and validity of the myopia susceptibility animal model. According to the construction method of the present invention, a mouse myopia susceptibility model can be successfully obtained.

[0047] The present invention also conducted comparative experiments to evaluate the effects of different exposure times on the construction of myopia susceptibility models in mice, such as Figure 2 As shown in (A), in the comparative experiment, mice on day 14 (P14) were exposed to full-spectrum white light and purple light for 1 week, 2 weeks, and 3 weeks respectively. It can be seen that the refractive power of mice exposed for 2 weeks was most significantly delayed; Figure 2 As shown in (B), after 3 weeks of exposure to full-spectrum white light and violet light and 10 days of form deprivation on day 14 (P14), the difference in binocular refractive power was not obvious; Figure 2 As shown in (C), after 3 weeks of exposure to full-spectrum white light and violet light and 10 days of form deprivation on day 14 (P14), there was no significant difference in the axial length growth of both eyes.

[0048] The present invention also conducted a comparative experiment to evaluate the visual function development, refractive development and myopia susceptibility of mice exposed to full-spectrum white light and violet light for 2 weeks starting from day 21 after birth (P21). Figure 3 As shown in (A), the light adaptation is 3.0cd / cm 2 Typical electroretinogram response diagram under the following conditions: N represents the baseline value, b represents the peak of the positive wave, and a represents the trough of the negative wave; Figure 3 (B) shows dark adaptation to 0.01 cd / cm 2 Typical electroretinogram responses under conditions such as Figure 3 (C) shows dark adaptation at 3.0 cd / cm 2 Typical electroretinogram responses under conditions such as Figure 3 (D) shows dark adaptation at 3.0 cd / cm 2 Typical graph of electroretinogram oscillatory wave response under the condition, N2 and N4 represent the second and fourth negative troughs, respectively, and P2 and P4 represent the second and fourth positive peaks, respectively; Figure 3 (E) shows the difference in binocular refractive power after mice were exposed to full-spectrum white light and violet light for 2 weeks starting from day 21 (P21) and then subjected to form deprivation. It can be seen that the contrast is not obvious; Figure 3 (F) shows the difference in eye axis growth between the two eyes after exposure to full-spectrum white light and violet light for 2 weeks starting from day 21 (P21) and then form deprivation. It can be seen that the contrast is not obvious; Figure 3(G) shows the light response amplitude of cone cells in mice exposed to full-spectrum white light and violet light for 2 weeks starting from day 21 (P21). It can be seen that the contrast is not obvious; Figure 3 (H) shows the light response amplitude of cone bipolar cells in mice exposed to full-spectrum white light and violet light for 2 weeks starting from day 21 (P21). It can be seen that the contrast is not obvious; Figure 3 As shown in (I), the light response amplitude of rod cells in mice exposed to full-spectrum white light and violet light for 2 weeks starting from day 21 (P21) is not obvious; Figure 3 (J) shows the light response amplitude of rod bipolar cells in mice exposed to full-spectrum white light and violet light for 2 weeks starting from day 21 (P21). It can be seen that the contrast is not obvious; Figure 3 (K) shows the light response amplitude of the inner nuclear layer cells of mice exposed to full-spectrum white light and violet light for 2 weeks starting from day 21 (P21). It can be seen that the contrast is not obvious.

[0049] The test results showed that the best model construction method was to expose mice to purple light for 2 weeks starting from day 14 (P14).

[0050] The above description is only an illustration of some preferred embodiments of the present disclosure and the technical principles used. Those skilled in the art should understand that the scope of the invention involved in the embodiments of the present disclosure is not limited to the technical solutions formed by the specific combination of the above-mentioned technical features, but should also cover other technical solutions formed by any combination of the above-mentioned technical features or their equivalent features without departing from the above-mentioned inventive concept. For example, the above-mentioned features are replaced with (but not limited to) technical features with similar functions disclosed in the embodiments of the present disclosure.

Claims

1. A developmental optical intervention system for inducing myopia susceptibility, characterized in that: include: A myopia susceptibility inducing device for inducing a myopia susceptibility state under ultraviolet light exposure; A myopia susceptibility assessment device is used to assess the myopia susceptibility state.

2. The developmental optical intervention system for inducing myopia susceptibility according to claim 1, characterized in that: The myopia susceptibility inducing device comprises an ultraviolet light exposure box, in which a short-wavelength visible ultraviolet light source is provided for exposure for 12 hours a day and for 14 days.

3. The developmental optical intervention system for inducing myopia susceptibility according to claim 2, characterized in that: The wavelength range of the short-wavelength visible violet light is 390-410 nm, and the illumination intensity is 20 lux.

4. The developmental optical intervention system for inducing myopia susceptibility according to claim 1, characterized in that: The myopia susceptibility assessment device includes a full-spectrum white light exposure box, an opaque eye mask for form deprivation, a refractive index test device, and a computer, wherein the refractive index test device and the computer are electrically connected; the full-spectrum white light exposure box is provided with full-spectrum white light for 12 hours per day for 14 days; the opaque eye mask is worn on one of the eyes after exposure to violet light or full-spectrum white light; the refractive index test device performs refractive index tests on the eye after and without form deprivation exposure, and obtains the refractive index difference between the two eyes after exposure to violet light and full-spectrum white light respectively through a computer, and finally compares the refractive index difference between the two eyes after exposure to violet light and full-spectrum white light to assess myopia susceptibility.

5. The developmental optical intervention system for inducing myopia susceptibility according to claim 4, characterized in that: During the refractive test, the eye wearing the opaque eyepatch serves as the deprivation eye, and the eye not wearing the opaque eyepatch serves as the non-deprivation eye. The opaque eyepatch is worn for 10 days. The refractive power difference between the deprivation eye and the non-deprivation eye after ultraviolet light exposure is 5.0 to 10.0 D. The refractive power difference between the deprivation eye and the non-deprivation eye after full-spectrum white light exposure is 0.0 to 5.0 D. If the refractive power difference is greater than 0.0 to 5.0 D, the patient is assessed as being susceptible to myopia.

6. A method for constructing a mouse myopia susceptibility model according to any one of claims 1 to 5, characterized in that: include: The construction method constructs a mouse myopia susceptibility model by exposing mice to ultraviolet light; the specific steps of the construction method include: The mice were placed in a violet light exposure box and continuously exposed to a short-wavelength visible violet light source with a wavelength range of 390-410 nm and a light intensity of 20 lux for 12 hours a day for 14 days, thereby constructing a mouse myopia susceptibility model.

7. The method for constructing a mouse myopia susceptibility model according to claim 6, wherein: include: The mice had just opened their eyes before the violet light exposure.

8. A mouse myopia susceptibility model, characterized by: The mouse myopia susceptibility model is obtained by constructing the system according to any one of claims 1 to 5.

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