Molecular marker closely linked with major QTL-qSHL-1B of coleoptile length under wheat alkali stress and application of molecular marker

By developing the molecular marker KNchr1B-691, which is closely linked to the long main effect QTL-qSHL-1B of the coleotid under wheat alkali stress, the complexity of genetic research on long-term wheat coleotids was solved, and rapid and accurate gene selection was achieved, wheat breeding efficiency and resource utilization were improved, and the breeding of high-yield new varieties was promoted.

CN120442854AActive Publication Date: 2025-08-08LUDONG UNIVERSITY
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Patent Information

Application Number
CN202510941756.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-09
Publication Date
2025-08-08
Estimated Expiration
2045-07-09

AI Technical Summary

Technical Problem

In alkaline soil environment, genetic research on wheat cosmolite length is complex, and the existing technology is difficult to accurately locate the gene location that controls the length of the cosmolite length, resulting in slow progress in wheat breeding and few studies on the 1B chromosome of cosmolite length QTL.

Method used

The molecular marker KNchr1B-691, which is closely linked to the long-main effect QTL-qSHL-1B of the coleotid under wheat-base stress, was developed to quickly and accurately detect whether wheat varieties have long-main effect QTL-qSHL-1B of the coleotid under wheat-base stress.

Benefits of technology

It provides fast and accurate gene selection tools, reduces the workload of phenotype identification, improves wheat breeding efficiency, saves costs, and provides excellent genetic resources for the selection and breeding of high-yield new varieties of wheat.

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Abstract

The invention discloses a molecular marker closely linked with wheat coleoptile length major QTL-qSHL-1B under alkali stress and application of the molecular marker, and belongs to the technical field of wheat molecular biotechnology and breeding. The molecular marker is KNchr1B-691 and is closely linked with the major QTL-qSHL-1B of the coleoptile length under alkali stress on a wheat 1B chromosome, the nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 4, and the molecular marker can be obtained by amplifying an upstream primer as shown in SEQ ID NO: 1 and a downstream primer as shown in SEQ ID NO: 2. The molecular marker KNchr1B-691 has the beneficial effects that the molecular marker KNchr1B-691 fully reflects the coleoptile length main effect QTL of the wheat variety (line) under alkali stress, whether the wheat variety (line) has the coleoptile length main effect or not under alkali stress can be quickly and accurately judged, and an excellent gene resource and a selection tool are provided for wheat yield trait molecular breeding.
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