A method for isolation and culture of mouse midpalatal suture stem cells

By isolating and culturing mouse palatal raphe tissue under a stereomicroscope and using a specific culture medium, the problems of difficult extraction and low purity of palatal raphe stem cells have been solved, achieving efficient isolation and culture of palatal raphe stem cells, and providing a foundation for the repair of congenital developmental malformations such as cleft palate.

CN120485111BActive Publication Date: 2026-04-14HOSPITAL OF STOMATOLOGY SUN YAT SEN UNIV
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Patent Information

Application Number
CN202510562283.7
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-04-30
Publication Date
2026-04-14
Estimated Expiration
2045-04-30

AI Technical Summary

Technical Problem

Existing technologies cannot effectively isolate and culture palatal raphe stem cells, and existing methods for extracting cranial suture stem cells are not suitable for palatal raphe stem cells, resulting in difficulties in extracting palatal raphe stem cells and low purity.

Method used

Maxillary tissue from 3-5 day old mice was isolated, preserving the palatal suture and the tissues on both sides. The tissues were cultured in a medium containing serum, antibiotics and growth factors. Palatal suture tissue was isolated under a stereomicroscope and passaged to ensure high purity and high stemness of palatal suture stem cells.

Benefits of technology

This study achieved efficient isolation and culture of palatal raphe stem cells, significantly improved the purity and stemness of palatal raphe stem cells, shortened experimental time, and provided a basis for the repair of congenital developmental malformations such as cleft palate.

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Abstract

The present application relates to a kind of mouse palatine midline stem cell separation and culture method, belong to stem cell separation culture technical field.The method of the present application includes the following steps: S1: separation 3-5 days old mouse maxillary tissue, retain palatine midline and its two sides tissue, tear two sides tissue along palatine midline, obtain unilateral palatine midline tissue;S2: with the medium containing serum, antibiotic and growth factor is cultured within 7 days in step S1 unilateral palatine midline tissue, obtain primary mouse palatine midline stem cell;The growth factor includes at least one of epidermal cell growth factor, fibroblast growth factor and transforming growth factor;S3: step S2 primary mouse palatine midline stem cell is subcultured, and obtain subculture mouse palatine midline stem cell.The method of the present application greatly shortens experimental time, and the palatine midline stem cell extracted is high, and it is convenient to operate, and it provides basis for the repair of cleft palate and other congenital developmental deformity.
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