A lipid-lowering synbiotic, its preparation method and application

Abstract

This invention relates to a lipid-lowering synbiotic, its preparation method, and its application, belonging to the field of egg-laying hen farming technology. The lipid-lowering synbiotic of this invention comprises the following components in parts by weight: 3-85 parts of traditional Chinese medicine extract, 1-25 parts of probiotics, 3-30 parts of prebiotics, 1-16 parts of enzyme preparation, and 4-55 parts of dietary therapy components. This synbiotic not only significantly improves the lipid metabolism of laying hens but also significantly prolongs the peak egg-laying period, increases the egg production rate, reduces cholesterol content in egg yolks, promotes calcium and phosphorus absorption, and reduces the paralysis rate in laying hens. It comprehensively improves the economic benefits of egg-laying hen farming.

Description

Technical Field

[0001] This invention relates to the field of egg-laying hen breeding technology, and in particular to a lipid-lowering synbiotic, its preparation method, and its application. Background Technology

[0002] Egg-laying hen farming is a crucial component of my country's livestock and poultry farming sector, with a consistently high market demand for eggs. As demand continues to rise, egg-laying hen farming is gradually shifting towards an intensive, high-density model. However, with increasing stocking density, problems related to fat metabolism in egg-laying hens are becoming increasingly prominent. Excessive fat accumulation not only leads to a decline in egg production performance but also easily induces conditions such as fatty liver syndrome, thereby increasing farming costs and reducing economic benefits.

[0003] Traditional layer hen feed primarily focuses on nutrient supply, but it has significant shortcomings in regulating the crucial aspect of fat metabolism in laying hens. For example, Meriwin adds artemisia powder to layer hen feed, reducing lipid deposition in the liver by inhibiting the expression of genes involved in liver lipid synthesis; Zhang Tao, on the other hand, reduces lipid synthesis by adding theaflavins to the feed, thereby lowering the expression of acetyl-CoA carboxylase, a key enzyme in liver lipid synthesis. While these methods have shown some effectiveness, their reliance on single components makes it difficult to comprehensively regulate layer hen fat metabolism through multiple pathways.

[0004] Chinese patent CN107823356A discloses a traditional Chinese medicine composition and its preparation method for preventing and treating fatty liver in laying hens. This method involves adding the powdered traditional Chinese medicine to the feed of laying hens, and it has achieved certain results in laying hen farming practice. Chinese patent CN105981983A discloses a selenium-enriched, lipid-lowering feed for laying hens and its preparation method. This method uses selenium-enriched raw materials and adds enzymes, amino acids, and traditional Chinese medicine to achieve the goal of lowering lipid levels in laying hens. While both methods have made some progress in improving the production performance of laying hens, they do not provide a comprehensive approach to regulating fat metabolism in laying hens. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of existing technologies and provide a lipid-lowering synbiotic, its preparation method, and its application. This synbiotic can not only effectively reduce the cholesterol content in egg yolks of laying hens, but also significantly improve the lipid metabolism of laying hens, prolong the peak laying period, and increase the egg production rate. Simultaneously, it reduces the cholesterol content in egg yolks, promotes calcium and phosphorus absorption in laying hens, and reduces the paralysis rate. This comprehensively improves the economic benefits of laying hen farming.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0007] In a first aspect, the present invention provides a lipid-lowering synbiotic, comprising the following components in parts by weight: 3-85 parts of traditional Chinese medicine extract, 1-25 parts of probiotics, 3-30 parts of prebiotics, 1-16 parts of enzyme preparation, and 4-55 parts of dietary therapy components.

[0008] This invention combines traditional Chinese medicine extracts, probiotics, prebiotics, enzyme preparations, and dietary components to obtain a synbiotic that can comprehensively regulate lipid metabolism in laying hens. This synbiotic not only significantly improves the lipid metabolism of laying hens but also significantly prolongs their peak egg production period, reduces the feed conversion ratio, increases egg production rate, and simultaneously lowers the cholesterol content in egg yolks, thus comprehensively improving the economic benefits of laying hen farming. This invention achieves its lipid-lowering effect through a comprehensive approach combining traditional Chinese medicine, probiotics, prebiotics, enzyme preparations, and dietary therapy.

[0009] As a preferred embodiment of the first aspect of the present invention, the traditional Chinese medicine extract includes the following components: Astragalus membranaceus, Salvia miltiorrhiza, Alisma plantago-aquatica, lotus leaf, red yeast rice, kapok flower, and mulberry leaf; wherein the weight ratio of lotus leaf, Alisma plantago-aquatica, and Salvia miltiorrhiza is 1:(1-3):(1-3); and the weight ratio of red yeast rice, kapok flower, and mulberry leaf is 1:(1-2):(1-2).

[0010] The Astragalus membranaceus used in this invention can regulate immunity, enhance liver metabolism, and improve the liver's efficiency in processing fat; Salvia miltiorrhiza can promote blood circulation, remove blood stasis, and help excrete fat metabolic waste, reducing lipid deposition in blood vessel walls and tissues; Alisma plantago-aquatica can promote diuresis and eliminate dampness, accelerating the excretion of excess water and lipids; lotus leaf contains lotus leaf alkaloids, which can inhibit fat absorption, promote decomposition, and increase satiety; red yeast rice contains monacolin K, which can inhibit key enzymes in cholesterol synthesis and reduce cholesterol production; kapok flower can clear heat and promote diuresis, optimizing the metabolic environment; flavonoids and other components in mulberry leaf can regulate the activity of lipid metabolism-related enzymes and assist in lowering lipids. This invention combines these traditional Chinese medicine components and found that they can produce a synergistic effect, which is beneficial for prolonging the peak egg production period of laying hens, reducing the feed conversion ratio, increasing the egg production rate of laying hens, reducing the cholesterol content in egg yolks, and improving the lipid metabolism status of laying hens. The effect is even better when lotus leaf, alisma, and salvia miltiorrhiza are combined in the following weight ratios: 1:(1-3):(1-3) and red yeast rice, kapok flower, and mulberry leaf in the following weight ratios: 1:(1-2):(1-2).

[0011] As a preferred embodiment of the first aspect of the present invention, the traditional Chinese medicine extract includes the following components: Astragalus membranaceus, Salvia miltiorrhiza, Alisma plantago-aquatica, lotus leaf, red yeast rice, kapok flower, and mulberry leaf; wherein the weight ratio of lotus leaf, Alisma plantago-aquatica, and Salvia miltiorrhiza is 1:2:2; and the weight ratio of red yeast rice, kapok flower, and mulberry leaf is 1:1:1.

[0012] The present invention has found that the effect of the synbiotic is best when the weight ratio of lotus leaf, alisma, and salvia miltiorrhiza is 1:2:2 and the weight ratio of red yeast rice, kapok flower, and mulberry leaf is 1:1:1.

[0013] As a preferred embodiment of the first aspect of the present invention, the herbal extract comprises the following components in parts by weight: 10-20 parts of Astragalus membranaceus, 5-15 parts of Salvia miltiorrhiza, 5-15 parts of Alisma plantago-aquatica, 5-10 parts of Nelumbo nucifera leaf, 3-8 parts of red yeast rice, 3-8 parts of Bombax ceiba flower, and 3-8 parts of mulberry leaf.

[0014] The herbal extract of this invention is prepared by the following method:

[0015] Powder 10-20 parts of Astragalus membranaceus, 5-15 parts of Salvia miltiorrhiza, 5-15 parts of Alisma plantago-aquatica, 5-10 parts of Nelumbo nucifera leaf, 3-8 parts of red yeast rice, 3-8 parts of Bombax ceiba flower, and 3-8 parts of mulberry leaf, mix them together, and place them in a supercritical CO2 extraction device. Extract for 2-3 hours under the conditions of extraction pressure of 20-30 MPa, extraction temperature of 40-50℃, and CO2 flow rate of 20-30 L / h. Collect the extract, and separate CO2 under reduced pressure to obtain the Chinese herbal extract.

[0016] As a preferred embodiment of the first aspect of the present invention, the probiotics comprise the following components in parts by weight: 3-7 parts of Lactobacillus acidophilus, 1-6 parts of Bifidobacterium animalis, 4-6 parts of Bacillus subtilis, and 2-5 parts of Clostridium butyricum.

[0017] This invention uses a combination of Lactobacillus acidophilus, Bifidobacterium animalis, Bacillus subtilis, and Clostridium butyricum, which has been shown to improve the intestinal microecology. On the one hand, it inhibits the growth of harmful bacteria and reduces the interference of harmful substances on lipid metabolism; on the other hand, it participates in bile acid metabolism, promotes cholesterol excretion, and regulates fat absorption and metabolism.

[0018] As a preferred embodiment of the first aspect of the present invention, the prebiotic comprises the following components in parts by weight: 4-12 parts of fructooligosaccharides, 3-8 parts of fructooligosaccharides, and 6-9 parts of inulin.

[0019] This invention selects fructooligosaccharides, lactulose, and inulin as prebiotics, which can promote the proliferation of probiotics, and the short-chain fatty acids produced by their fermentation inhibit the synthesis of fatty acids in the liver.

[0020] As a preferred embodiment of the first aspect of the present invention, the enzyme preparation comprises the following components in parts by weight: 1-4 parts of lipase, 2-7 parts of protease and 2-4 parts of cellulase.

[0021] This invention utilizes lipase to break down feed fat into fatty acids and glycerol, which facilitates absorption and utilization and reduces fat accumulation; protease to break down protein, providing the body with sufficient amino acids and ensuring normal metabolism; and cellulase to break down cellulose, increasing energy sources and preventing excess energy from being converted into fat.

[0022] As a preferred embodiment of the first aspect of the present invention, the dietary therapy components include the following components in parts by weight: 5-15 parts whey powder, 10-20 parts oat flour, 6-10 parts kelp powder and 4-8 parts sea buckthorn seed meal.

[0023] This invention utilizes whey powder, which contains high-quality protein and conjugated linoleic acid (CLA). The former maintains the body's metabolism, while the latter inhibits the proliferation and differentiation of adipocytes and promotes fatty acid oxidation. Oat flour is rich in dietary fiber, reducing cholesterol absorption and increasing satiety; sodium alginate in kelp powder binds to cholesterol and is excreted from the body; and sea buckthorn seed meal contains various bioactive components that help regulate lipid metabolism.

[0024] Secondly, the present invention provides a method for preparing the lipid-lowering synbiotic described in the first aspect, comprising the following steps:

[0025] (1) Extract the Chinese herbal composition to obtain a Chinese herbal extract;

[0026] (2) Cultivate probiotics to obtain probiotic culture;

[0027] (3) After mixing and freeze-drying the probiotic culture with prebiotics, a probiotic-prebiotic complex was prepared.

[0028] (4) Mix the Chinese herbal extract, probiotic prebiotic complex, enzyme preparation and dietary therapy components to obtain the lipid-lowering synbiotic.

[0029] As a preferred embodiment of the second aspect of the present invention, the extraction method in step (1) is as follows:

[0030] The traditional Chinese medicine composition was pulverized and placed in a supercritical extraction device for extraction. The extract was collected and CO2 was separated under reduced pressure. The extraction parameters were: extraction pressure 20-30 MPa, extraction temperature 40-50℃, CO2 flow rate 20-30 L / h, and extraction time 2-3 hours.

[0031] Thirdly, the present invention provides the application of the lipid-lowering synbiotic described in the first aspect in the preparation of laying hen feed.

[0032] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0033] (1) The invention achieves lipid-lowering effects through comprehensive conditioning using traditional Chinese medicine, probiotics, prebiotics, enzyme preparations and dietary therapy;

[0034] (2) The traditional Chinese medicine formula selected in this invention, including Salvia miltiorrhiza, Astragalus membranaceus, red yeast rice, lotus leaf, Alisma plantago-aquatica, Bombax ceiba and mulberry leaf, can effectively regulate lipid metabolism. The formula is prepared by supercritical extraction, which can effectively reduce the loss of efficacy.

[0035] (3) The present invention selects Lactobacillus acidophilus, Bifidobacterium animalis, Bacillus subtilis and Clostridium butyricum as compound probiotics, which can effectively improve the intestinal flora of laying hens, enhance the immunity of laying hens and reduce lipid synthesis.

[0036] (4) The present invention mixes Lactobacillus acidophilus, Bifidobacterium animalis, Bacillus subtilis, Clostridium butyricum with fructooligosaccharides, lactulose and inulin and freeze-drys to make a probiotic and prebiotic complex, which can effectively prolong the survival time of probiotics. After the probiotics enter the intestine, the prebiotics can also promote the proliferation of probiotics.

[0037] (5) The present invention also adds dietary therapy components to the prebiotics, which can further regulate lipid metabolism through dietary nutrition.

[0038] Therefore, this invention can comprehensively reduce lipid accumulation, prevent fatty liver syndrome in laying hens, improve immunity, effectively improve egg quality, reduce cholesterol in egg yolks, extend the peak egg-laying period of laying hens, and effectively improve the economic benefits of the breeding industry. Detailed Implementation

[0039] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0040] The *Lactobacillus acidophilus* strain of this invention was purchased from the Beina Chuanglian Biotechnology Research Institute, strain number: BNCC 185342; the *Bifidobacterium animalis* strain of this invention was purchased from the China Industrial Microbial Culture Collection Center, strain number: CICC 6174; the *Bacillus subtilis* strain of this invention was purchased from the China Industrial Microbial Culture Collection Center, strain number: CICC 10260; and the *Clostridium butyricum* strain of this invention was purchased from the China Industrial Microbial Culture Collection Center, strain number: CICC10390.

[0041] The lipase used in this invention was purchased from Shanghai Yuanye Biotechnology Co., Ltd., with an enzyme activity of 300,000 U / g; the protease used in this invention was purchased from Shanghai Yuanye Biotechnology Co., Ltd., with an enzyme activity of 150 u / mg; and the cellulase used in this invention was purchased from Shanghai Yuanye Biotechnology Co., Ltd., with an enzyme activity of 400 u / mg.

[0042] The whey powder, oat flour, kelp powder, and sea buckthorn seed meal used in this invention were all purchased from the market.

[0043] Boen 4% Laying Hen Compound Premixed Feed H043 was purchased from Boen Group Co., Ltd.

[0044] The components and their weight parts of the lipid-lowering synbiotics in Examples 1-5 are shown in the table below:

[0045] Table 1:

[0046]

[0047]

[0048] Example 1: The preparation method of the lipid-lowering synbiotic is as follows:

[0049] (1) Extracting Chinese herbal medicines to obtain Chinese herbal medicine extracts, the specific steps are as follows:

[0050] Astragalus membranaceus, Salvia miltiorrhiza, Alisma plantago-aquatica, lotus leaf, red yeast rice, kapok flower, and mulberry leaf were mixed according to the weight parts in Table 1, pulverized, and placed in a supercritical CO2 extraction device. The mixture was extracted for 3 hours under the conditions of extraction pressure of 20 MPa, extraction temperature of 40℃, and CO2 flow rate of 20 L / h. The extract was collected, and CO2 was separated under reduced pressure to obtain the Chinese herbal extract.

[0051] (2) Cultivate the probiotics to obtain a probiotic culture. The specific steps are as follows:

[0052] Lactobacillus acidophilus and Bifidobacterium animalis were inoculated into MRS medium and cultured anaerobically at 37°C for 48 h; Bacillus subtilis was inoculated into LB medium and fermented aerobically at 37°C for 24 h; and Clostridium butyricum was inoculated into Clostridium-enriched medium and cultured anaerobically at 37°C for 72 h. After centrifugation, the bacterial cells were collected and washed three times with sterile physiological saline to prepare single bacterial suspensions, thus obtaining the various probiotic cultures.

[0053] (3) Mix each probiotic culture with prebiotics according to the weight parts in Table 1, freeze dry, and prepare a probiotic-prebiotic complex.

[0054] (4) Mix the enzyme preparations thoroughly according to the weight proportions in Table 1 to prepare a compound enzyme preparation;

[0055] (5) Mix the Chinese herbal extract, probiotic prebiotic complex, enzyme preparation and dietary therapy components evenly to obtain the lipid-lowering synbiotic.

[0056] The difference between Example 6 and Example 1 is that Astragalus membranaceus is missing, and the missing part is made up with water; otherwise, it is the same as Example 1.

[0057] The difference between Example 7 and Example 1 is that Poria cocos is used instead of Alisma plantago-aquatica, otherwise the same as in Example 1.

[0058] The difference between Example 8 and Example 1 is that the lotus leaves are missing, and the missing part is made up by water; otherwise, it is the same as Example 1.

[0059] The difference between Example 9 and Example 1 is that the red yeast rice is replaced with the divine song, otherwise it is the same as Example 1.

[0060] The difference between Example 10 and Example 1 is that the kapok flowers are missing, and the missing part is made up by water; otherwise, it is the same as Example 1.

[0061] The difference between Example 11 and Example 1 is that dandelion is used instead of kapok flower, otherwise the same as Example 1.

[0062] The difference between Example 12 and Example 1 is that mulberry leaves are missing, and the missing part is made up by water; otherwise, it is the same as Example 1.

[0063] The difference between Example 13 and Example 1 is that Lactobacillus acidophilus is missing, and the missing part is made up by water; otherwise, it is the same as Example 1.

[0064] The difference between Example 14 and Example 1 is that Bifidobacterium animalis is replaced with Lactobacillus paracasei, otherwise the same as in Example 1.

[0065] The difference between Example 15 and Example 1 is that kelp powder is missing, and the missing part is made up by water; otherwise, it is the same as Example 1.

[0066] The difference between Example 16 and Example 1 is that peanut meal is used instead of sea buckthorn seed meal, otherwise the same as Example 1.

[0067] The difference between Comparative Example 1 and Example 1 is that the Chinese herbal extract is missing, and the missing part is made up by water; otherwise, it is the same as Example 1.

[0068] The difference between Comparative Example 2 and Example 1 is that Comparative Example 2 lacks probiotics, and the missing part is made up by water; otherwise, it is the same as Example 1.

[0069] The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 lacks prebiotics, and the missing part is made up by water; otherwise, it is the same as Example 1.

[0070] The difference between Comparative Example 4 and Example 1 is that the enzyme preparation is missing, and the missing part is made up by water; otherwise, it is the same as Example 1.

[0071] The difference between Comparative Example 5 and Example 1 is that Comparative Example 5 lacks the dietary therapy components, and the missing parts are made up by water; otherwise, it is the same as Example 1.

[0072] Application example: The lipid-lowering synbiotic of the present invention is used to prepare laying hen feed.

[0073] Application Examples 1-16 used the synbiotics prepared in Examples 1-16, while Application Examples 1-5 used the synbiotics prepared in Comparative Examples 1-5. The difference between the blank control group and Application Example 1 was that no lipid-lowering synbiotics were added.

[0074] Application examples and comparative application examples are shown in the table below:

[0075] Table 2:

[0076]

[0077] The laying hen feeds prepared in the application examples and control examples were used in a laying hen farm in Sihui City to conduct a late-laying laying hen breeding experiment. 77-week-old Hy-Line Brown laying hens of similar weight and good health were selected as the research subjects. The hens were randomly divided into 22 groups (21 experimental groups and 1 blank control group; the experimental groups were fed the feeds of application examples 1-16 and control examples 1-5, respectively, while the blank control group was fed a basal diet throughout the experiment), with 60 hens in each group (each group was divided into 3 subgroups of 20 hens each, for parallel testing). The experiment was conducted indoors with good ventilation for 42 days. After the experiment, various indicators were measured, and the average values ​​were taken.

[0078] Test method:

[0079] 1. Egg production rate, laying hen paralysis rate, and broken egg rate were analyzed using statistical methods;

[0080] Egg production rate % = Number of eggs laid ÷ Number of chickens × 100%;

[0081] Laying hen paralysis rate % = Number of paralyzed hens ÷ Total number of hens × 100%

[0082] Broken egg rate % = Number of broken eggs ÷ Total number of eggs × 100%

[0083] 2. Egg yolk cholesterol testing shall be performed in accordance with GB5009.128-2016;

[0084] 3. Egg yolk color was determined by colorimetry: The DSM egg yolk colorimetric fan was used to divide the color into 1 to 15 levels. The higher the level, the more orange-red the egg yolk color is. The sample was compared side by side with the colorimetric card under D65 light source to match the closest color level.

[0085] 4. TC (cholesterol), TG (triglycerides), calcium, and phosphorus were detected using reagent kits from Changchun Huili Biotechnology Co., Ltd., and the serum from laying hens was tested according to the kit instructions.

[0086] 5. The taste was evaluated using a sensory method according to GB2749-2015: Eggs were placed in water, heated to a boil for 10 minutes, cooled, and the shells were removed. Evaluators then scored the eggs, as shown in the table below:

[0087] Table 3

[0088]

[0089] The test results are shown in the table below:

[0090] Table 4

[0091]

[0092]

[0093] Table 5

[0094]

[0095] As seen in Application Examples 1-5, the weight ratios of lotus leaf, Alisma plantago-aquatica, and Salvia miltiorrhiza, as well as the weight ratios of red yeast rice, kapok flower, and mulberry leaf, affect the efficacy of the lipid-lowering synbiotic. In particular, when the weight ratio of lotus leaf, Alisma plantago-aquatica, and Salvia miltiorrhiza is 1:(1-3):(1-3), and the weight ratio of red yeast rice, kapok flower, and mulberry leaf is 1:(1-2):(1-2), the lipid-lowering effect of the synbiotic is more pronounced. Especially when the weight ratio of lotus leaf, Alisma plantago-aquatica, and Salvia miltiorrhiza is 1:2:2, and the weight ratio of red yeast rice, kapok flower, and mulberry leaf is 1:1:1 (Example 1), the lipid-lowering effect of the synbiotic is optimal. Although the weight ratios in Application Examples 2 and 3 are within the above ranges, their effects are not as good as in Example 1. Although the weight proportions of each herbal component in Application Examples 4 and 5 are within the selection range of this invention, their effects are also not as good as in Example 1 because the weight ratios of lotus leaf, Alisma plantago-aquatica, and Salvia miltiorrhiza, as well as the weight ratios of red yeast rice, kapok flower, and mulberry leaf are not within the ranges defined by this invention.

[0096] As can be seen from Application Examples 6-12, the absence or substitution of any one of the traditional Chinese medicine ingredients prevents the synergistic effect of the herbal combination in this invention from being produced, thus affecting the efficacy of the lipid-lowering synbiotic. Therefore, as shown in Application Examples 6-12, the Astragalus membranaceus, lotus leaf, Alisma plantago-aquatica, Salvia miltiorrhiza, red yeast rice, Bombax ceiba, and mulberry leaf selected in this invention produce a synergistic effect for lipid-lowering, and the absence or substitution of any one of them will destroy their synergistic lipid-lowering effect.

[0097] As can be seen from application examples 13-16, the lack of any one of the components in the probiotics and dietary therapy components prevents the combination of the present invention from producing a synergistic effect, thereby affecting the efficacy of the lipid-lowering synbiotic.

[0098] Compared with application examples 1-5, the lack of one of the following components—traditional Chinese medicine extract, probiotics, probiotics, enzyme preparations, or dietary therapy components—results in the inability of the formulation of the present invention to produce a synergistic effect, thereby affecting the efficacy of the lipid-lowering synbiotic.

[0099] The experimental results show that this lipid-lowering prebiotic can effectively prolong the peak laying period, increase the egg production rate of laying hens, improve the yolk color of eggs, reduce egg yolk cholesterol, reduce serum cholesterol and serum triglycerides, reduce the paralysis rate and broken egg rate of laying hens, and improve the economic benefits of laying hen farming. We also surprisingly found that this lipid-lowering prebiotic promotes the absorption efficiency of calcium and phosphorus in feed, and while reducing the addition of calcium and phosphorus in feed, it also has a certain protective effect on the environment. In addition, this prebiotic also improves the taste of eggs, and enhances the flavor and nutritional value of eggs.

[0100] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A lipid-lowering synbiotic, characterized in that, The lipid-lowering synbiotic is composed of the following components in parts by weight: Astragalus membranaceus 10-20 parts, Salvia miltiorrhiza 5-15 parts, Alisma plantago-aquatica 5-15 parts, Nelumbo nucifera leaf 5-10 parts, red yeast rice 3-8 parts, Bombax ceiba flower 3-8 parts, mulberry leaf 3-8 parts; Lactobacillus acidophilus 3-7 parts, Bifidobacterium animalis 1-6 parts, Bacillus subtilis 4-6 parts, Clostridium butyricum 2-5 parts; 4-12 parts fructooligosaccharides, 6-9 parts inulin; Lipase 1-4 parts, protease 2-7 parts, cellulase 2-4 parts; 5-15 parts whey powder, 10-20 parts oat flour, 6-10 parts kelp powder, and 4-8 parts sea buckthorn seed meal.

2. The lipid-lowering synbiotic as described in claim 1, characterized in that, The weight ratio of lotus leaf, Alisma plantago-aquatica, and Salvia miltiorrhiza is: lotus leaf: Alisma plantago-aquatica: Salvia miltiorrhiza = 1: (1-3): (1-3); the weight ratio of red yeast rice, kapok flower, and mulberry leaf is: red yeast rice: kapok flower: mulberry leaf = 1: (1-2): (1-2).

3. A method for preparing the lipid-lowering synbiotic as described in claim 1, characterized in that, Includes the following steps: (1) Astragalus membranaceus, Salvia miltiorrhiza, Alisma plantago-aquatica, lotus leaf, red yeast rice, kapok flower and mulberry leaf were extracted to obtain Chinese herbal extracts; (2) Lactobacillus acidophilus, Bifidobacterium animalis, Bacillus subtilis and Clostridium butyricum were cultured separately to obtain probiotic cultures; (3) The probiotic culture is mixed with fructooligosaccharides and inulin and freeze-dried to prepare a probiotic prebiotic complex; (4) Mix the Chinese herbal extract, probiotic prebiotic complex, lipase, protease, cellulase, whey powder, oat flour, kelp powder and sea buckthorn seed meal to obtain the lipid-lowering synbiotic.

4. The preparation method according to claim 3, characterized in that, The extraction method in step (1) is as follows: Astragalus membranaceus, Salvia miltiorrhiza, Alisma plantago-aquatica, lotus leaf, red yeast rice, kapok flower, and mulberry leaf are pulverized and placed in a supercritical extraction device for extraction. The extract is collected and CO2 is separated under reduced pressure. The extraction parameters are: extraction pressure 20-30 MPa, extraction temperature 40-50℃, CO2 flow rate 20-30 L / h, and extraction time 2-3 hours.

5. The application of the lipid-lowering synbiotic as described in claim 1 or 2 in the preparation of laying hen feed, characterized in that, The application is to increase the calcium and phosphorus content in the serum of laying hens.

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