Application of plasmodiophora brassicae Pb035 gene in prevention and control of clubroot of cruciferae crops
By targeting the inhibition of the Pb035 gene of the root knot fungus and silencing its expression using RNA interference technology, the limitations of existing prevention and control methods were overcome, and efficient prevention and control of root knot disease in cruciferous crops was achieved, thereby enhancing crop resistance and reducing environmental pollution risks.
Patent Information
- Application Number
- CN202511121512.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-12
- Publication Date
- 2025-09-09
- Estimated Expiration
- 2045-08-12
AI Technical Summary
Existing methods for preventing and controlling clubroot in cruciferous crops, such as chemical control, soil management, biological control, and breeding of disease-resistant varieties, have limitations and are unable to effectively prevent and control clubroot. In addition, the long-term use of chemical pesticides leads to drug resistance and environmental pollution, biological control is restricted by environmental conditions, and the effectiveness of disease-resistant varieties is limited.
RNA interference technology is used to target and inhibit the Pb035 gene of root knot fungus. By constructing a recombinant vector and using Agrobacterium-mediated method or root irrigation to apply RNA interference fragments, the expression of Pb035 gene is silenced, and its expression level in cruciferous crops is reduced, thereby achieving the prevention and treatment of root knot disease.
Significantly enhance the resistance of cruciferous crops to clubroot, reduce the disease index and pathogen biomass, provide long-lasting and efficient prevention and control measures, and reduce the environmental risks of chemical pesticide use.