Acid-resistant element from escherichia coli and application of acid-resistant element

By introducing the HdeB and GlsA genes of Escherichia coli into Corynebacterium glutamicum, a recombinant vector was constructed to enhance its survival ability in acidic environments, solving the growth problem of Corynebacterium glutamicum under acidic stress and achieving a significant increase in growth rate and stability of the transformation effect.

CN120624470APending Publication Date: 2025-09-12JILIN UNIVERSITY
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Patent Information

Application Number
CN202510813992.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-06-18
Publication Date
2025-09-12

AI Technical Summary

Technical Problem

In the existing technology, the growth rate and metabolic activity of Corynebacterium glutamicum are affected under acidic stress environment, resulting in a decrease in product yield and an extension of the fermentation cycle. In addition, its own acid resistance mechanism is imperfect and the transformation effect is unstable.

Method used

The HdeB and GlsA genes from Escherichia coli were introduced as exogenous acid-resistant elements, and a recombinant vector was constructed through heterologous expression and introduced into Corynebacterium glutamicum to enhance its survival ability in acidic environment.

Benefits of technology

It significantly improved the growth ability of Corynebacterium glutamicum in acidic environments, especially at pH 5.5, where the growth rate increased by more than 20 times, expanded the application range of acid-resistant elements, and simplified the operating procedures.

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Abstract

The invention discloses an acid-resistant element derived from Escherichia coli and application of the acid-resistant element. The acid-resistant elements are respectively HdeB and GlsA, and the acid-resistant elements are constructed on an expression vector and are introduced into corynebacterium glutamicum to obtain an acid-resistant engineering strain. A research result shows that the acid-resistant element has a good effect on improving the acid resistance of corynebacterium glutamicum in an acid environment such as a condition that the pH value is 5.5, and the application potential of the acid-resistant element in fermentation strain modification is proved.
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