Apolipoprotein E determination kit and preparation method thereof
By adding EDTA2Na and mannitol to the apolipoprotein E assay kit and optimizing the amount of PEG6000, the issues of kit opening stability and blank absorbance were resolved, achieving higher stability and lower absorbance, thus ensuring the accuracy and speed of the assay results.
CN120908455APending Publication Date: 2025-11-07JIANGSU MAIYUAN BIOTECHNOLOGY CO LTD
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Patent Information
- Application Number
- CN202510937098.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-08
- Publication Date
- 2025-11-07
AI Technical Summary
Technical Problem
Existing apolipoprotein E assay kits have shortcomings in terms of opening stability and blank absorbance, resulting in inaccurate and unstable measurement results.
Method used
The stability of the reagents and the reaction rate were improved by adding stabilizers EDTA2Na and mannitol to the kit, optimizing the amount of polymeric accelerator PEG6000, adjusting the ratio of reaction promoters and surfactants, and optimizing the preparation process.
Benefits of technology
The reagent kit has improved opening stability and reduced blank absorbance, ensuring the accuracy and speed of the measurement results. All performance indicators are superior to industry standards.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention provides an apolipoprotein E determination kit and a preparation method thereof, the apolipoprotein E determination kit comprises a reagent 1, a reagent 2 and a calibrator / quality control product, the reagent 1 comprises the following components: a PIPES buffer solution, an ionic electrolyte, a polymer accelerator, a reaction accelerator, a surfactant, a stabilizer and a preservative; and the reagent 2 comprises the following components: an anti-ApoE antibody solution, a PIPES buffer solution, glycerol, an ionic electrolyte, a polymer accelerator, a reaction accelerator, a surfactant, a stabilizer and a preservative. The kit is optimized by adding EDTA2Na and mannitol, so that the opening test is more stable. And the preparation process of the reagent 2 and the PEG 6000 sample adding amount are adjusted. The kit is quick in reaction, high in accuracy and lower in blank absorbance.
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