Small heat shock protein gene RcHsp20-5 of Chinese rose and application of small heat shock protein gene RcHsp20-5 in regulation and control of number of floral organs

By cloning the rose RcHsp20-5 gene and constructing a recombinant expression vector to transform Arabidopsis thaliana, the number of floral organs was regulated, revealing the potential role of heat shock proteins in floral diversity.

CN120944914APending Publication Date: 2025-11-14NORTHEAST FORESTRY UNIV
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Patent Information

Application Number
CN202511445896.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-10-11
Publication Date
2025-11-14

AI Technical Summary

Technical Problem

Current research on the molecular mechanisms of rose flower shape lacks a deep understanding of the regulation of floral organ numbers by heat shock proteins, and there is insufficient research on gene regulation that affects flower shape diversity.

Method used

The RcHsp20-5 gene of rose was cloned, a recombinant expression vector was constructed and transformed into the model plant Arabidopsis thaliana. Transgenic plants with high expression were obtained through stable genetic transformation, and the changes in the number of floral organs were observed.

Benefits of technology

The discovery of an increase or decrease in the number of petals and a change or decrease in the number of stamens in Arabidopsis thaliana demonstrates that the RcHsp20-5 gene has the function of regulating the number of floral organs and may affect the number of floral organs in roses.

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Abstract

The invention discloses a Chinese rose small heat shock protein family gene RcHsp20-5 and application thereof in regulation and control of the number of floral organs, and belongs to the field of plant genetic engineering breeding. The RcHsp20-5 gene is separated and cloned from a Chinese rose variety YuLinglong on the basis of a plant gene cloning technology, the nucleotide sequence of the RcHsp20-5 gene is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2. The expression protein of the RcHsp20-5 gene belongs to a CI subfamily. An overexpression vector is constructed, the gene is introduced into arabidopsis thaliana by using a floral immersion method, a high-expression transgenic plant is obtained, and a result shows that the RcHsp20-5 can influence petal symmetry and the number of floral organs of the transgenic arabidopsis thaliana. The Chinese rose RcHsp20-5 provided by the invention may be a potential gene influencing the number of Chinese rose organs, and provides a reference for researching the molecular regulation mechanism of the diversity of ornamental plant flower types.
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